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Gga3 (NM_001108304) Rat Untagged Clone
CAT＃: RN200582
Gga3 (untagged ORF) - Rat golgi associated, gamma adaptin ear containing, ARF binding protein 3 (Gga3), (10 ug)
CNY 8,460.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200582 representing NM_001108304
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGCGGAAGGGGAGAGCCTGGAATCCTGGCTGAATAAAGCTACCAATCCTTCCAACCGCCAGG AAGACTGGGAATACATCATTGGCTTCTGTGATCAGATCAACAAAGAGCTTGAAGGGCCCCAGATTGCTGT GCGACTCCTGGCTCACAAGATCCAGTCTCCACAGGAATGGGAGGCAGTGCAGGCCCTCACGGTGCTGGAG GCGTGTATGAAGAATTGCGGCAGGAGACTTCATAATGAAGTGGGAAAGTTCCGATTTTTGAATGAATTAA TCAAAGTCGTCTCTCCAAAGTACTTGGGAGACAGGGTGTCTGAAAAGGTGAAGGCTAAGGTCATCGAGTT GCTTTTTAGCTGGACACTGGCCTTGCCGGAAGAAGCAAAGATCAAAGACGCCTACCATATGTTGAAGAGA CAAGGCATAGTGCAGTCGGACCCCCCTATCCCCATGGACAGAACACTGATCCCCTCTCCTCCACCTCGTC CCAAAAACCCTGTTTTTGATGATGAGGAAAAATCCAAGCTCTTAGCCAAGCTGCTGAGAAGCAAGAACCC AGATGACTTACAAGAAGCCAACCAACTCATCAAGTCCATGGTGAAGGAAGATGAGGCGCGCATCCAGAAA GTGACCAAGCGCCTACACACGTTGGAGGAAGTTAACAACAATGTCAAGCTGCTCCATGAGATGCTTCTTC ACTACAGCCAAGAGTTCTCATCAGAGGCCGACAAAGAGCTCATGAAGGAGCTCTTTGATCGTTGTGAGAA TAAGAGACGGACATTATTTAAACTAGCCAGTGAGACAGAAGACAATGACAATAGTTTGGGGGACATCCTG CAGGCCAGTGACAACCTGTCCAGGGTCATCAACTCTTACAAAACAATTATTGAAGGGCAGATCATCAATG GTGAGGTGACCACCTCAACCGTGCCTGACTCTGAAGGAAATAGTCACTGCGGTAACCAAGGCGCCCTCAT CGACCTTGCCGAGTTGGACACCCCCAGCAGCTCATCCCCAGTGTTGGCCCCAGCACCTGCCCCACCCACC TCAGGCATCCCTATCCTTCCCCCACCTCCCCAAACCTCCGGGCCTCCACGAAGCCGTTCATCCAGCCAGG CTGAGGCTCCCTCAGGGCCTGACAGCACGAACAATGCCCTCTCCTTGCTGGATGAGGAGCTCCTCTGTCT AGCTCCTGTGGTCCCAGCGAGCGCAGTGACCCACAGCACTGGTTCCTTTACGTTCTCTTCTGGACCTGCC CCAGCCTTGGTTCCAAAGGCTGAGCCCGAAGGCCCAGAGTACCCCAGCTCCAGCATCTCACACCGCCTGG ATGCCCTTGATCAGCTTCTGGAAGAGGCCAAAGTGACTTCAGGCCTGGTGAAACCTGTTTCTTGCTTCTC TCCTGGGCCCACCGCCTCCCCACTGCTTCCTGCCTCTACCCCAGCCAGGCCTCTCCTGCCCTTCTCAACT GGGCCCGGAAGTCCTCTCTTCCAGTCGCCAGCCTTCCAGTCCCAAGGCAGCCCTCAGAAGGGCCCAGAGC TCTCCCTGGCTAGTGTCCATGTGCCCCTGGAATCGATCAAGCCTAGCAGTGCCCTTCCTGTGACAGCCTA TGATAAAAATGGCTTCCGCATCCTTTTCCACTTTGCTAAGGAGTGTCCGCCAGGACGGCCTGATGTGCTG GTGGTGGTGGTTTCCATGCTGAATACAGCTCCCTTGCCAGTCAAGAGTATAGTGCTACAGGCCGCAGTGC CCAAGTCAATGAAAGTGAAGTTGCAGCCACCCTCTGGGACAGAACTTTCTCCATTTAGTCCCATCCAGCC ACCTGCAGCCATCACCCAGGTCATGCTGCTGGCCAATCCAATGAAGGAAAAGGTACGGCTTCGGTATAAA TTGACCTTTGCTCTGGGGGAGCAGCTGAGCACAGAGCTGGGTGAAGTGGACCAGTTTCCTCCTGTGGAGC AGTGGGGGAACCTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001108304 |
| Insert Size | 1977 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001108304.1, NP_001101774.1 |
| RefSeq Size | 4565 bp |
| RefSeq ORF | 1977 bp |
| Locus ID | 360658 |
| Gene Summary | Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Involved in BACE1 transport and sorting as well as regulation of BACE1 protein levels. Regulates retrograde transport of BACE1 from endosomes to the trans-Golgi network via interaction through the VHS motif and dependent of BACE1 phosphorylation. Modulates BACE1 protein levels intedepently of the interaction between VHS domain and DXXLL motif through recognition of ubiquitination (By similarity). Key player in a novel DXXLL-mediated endosomal sorting machinery to the recycling pathway that targets NTRK1 to the plasma membrane (PubMed:26446845).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200582 | Gga3 (Myc-DDK-tagged ORF) - Rat golgi associated, gamma adaptin ear containing, ARF binding protein 3 (Gga3), (10 ug) |
CNY 8,460.00 |
|
| RR200582L3 | Lenti ORF clone of Gga3 (Myc-DDK-tagged ORF) - Rat golgi associated, gamma adaptin ear containing, ARF binding protein 3 (Gga3), (10 ug) |
CNY 10,260.00 |
|
| RR200582L4 | Lenti ORF clone of Gga3 (mGFP-tagged ORF) - Rat golgi associated, gamma adaptin ear containing, ARF binding protein 3 (Gga3), (10 ug) |
CNY 11,310.00 |
