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Gga3 (NM_001108304) Rat Tagged ORF Clone
CAT＃: RR200582
- TrueORF®
Gga3 (Myc-DDK-tagged ORF) - Rat golgi associated, gamma adaptin ear containing, ARF binding protein 3 (Gga3), (10 ug)
"NM_001108304" in other vectors (3)
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CNY 8,460.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200582 representing NM_001108304
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGCGGAAGGGGAGAGCCTGGAATCCTGGCTGAATAAAGCTACCAATCCTTCCAACCGCCAGG AAGACTGGGAATACATCATTGGCTTCTGTGATCAGATCAACAAAGAGCTTGAAGGGCCCCAGATTGCTGT GCGACTCCTGGCTCACAAGATCCAGTCTCCACAGGAATGGGAGGCAGTGCAGGCCCTCACGGTGCTGGAG GCGTGTATGAAGAATTGCGGCAGGAGACTTCATAATGAAGTGGGAAAGTTCCGATTTTTGAATGAATTAA TCAAAGTCGTCTCTCCAAAGTACTTGGGAGACAGGGTGTCTGAAAAGGTGAAGGCTAAGGTCATCGAGTT GCTTTTTAGCTGGACACTGGCCTTGCCGGAAGAAGCAAAGATCAAAGACGCCTACCATATGTTGAAGAGA CAAGGCATAGTGCAGTCGGACCCCCCTATCCCCATGGACAGAACACTGATCCCCTCTCCTCCACCTCGTC CCAAAAACCCTGTTTTTGATGATGAGGAAAAATCCAAGCTCTTAGCCAAGCTGCTGAGAAGCAAGAACCC AGATGACTTACAAGAAGCCAACCAACTCATCAAGTCCATGGTGAAGGAAGATGAGGCGCGCATCCAGAAA GTGACCAAGCGCCTACACACGTTGGAGGAAGTTAACAACAATGTCAAGCTGCTCCATGAGATGCTTCTTC ACTACAGCCAAGAGTTCTCATCAGAGGCCGACAAAGAGCTCATGAAGGAGCTCTTTGATCGTTGTGAGAA TAAGAGACGGACATTATTTAAACTAGCCAGTGAGACAGAAGACAATGACAATAGTTTGGGGGACATCCTG CAGGCCAGTGACAACCTGTCCAGGGTCATCAACTCTTACAAAACAATTATTGAAGGGCAGATCATCAATG GTGAGGTGACCACCTCAACCGTGCCTGACTCTGAAGGAAATAGTCACTGCGGTAACCAAGGCGCCCTCAT CGACCTTGCCGAGTTGGACACCCCCAGCAGCTCATCCCCAGTGTTGGCCCCAGCACCTGCCCCACCCACC TCAGGCATCCCTATCCTTCCCCCACCTCCCCAAACCTCCGGGCCTCCACGAAGCCGTTCATCCAGCCAGG CTGAGGCTCCCTCAGGGCCTGACAGCACGAACAATGCCCTCTCCTTGCTGGATGAGGAGCTCCTCTGTCT AGCTCCTGTGGTCCCAGCGAGCGCAGTGACCCACAGCACTGGTTCCTTTACGTTCTCTTCTGGACCTGCC CCAGCCTTGGTTCCAAAGGCTGAGCCCGAAGGCCCAGAGTACCCCAGCTCCAGCATCTCACACCGCCTGG ATGCCCTTGATCAGCTTCTGGAAGAGGCCAAAGTGACTTCAGGCCTGGTGAAACCTGTTTCTTGCTTCTC TCCTGGGCCCACCGCCTCCCCACTGCTTCCTGCCTCTACCCCAGCCAGGCCTCTCCTGCCCTTCTCAACT GGGCCCGGAAGTCCTCTCTTCCAGTCGCCAGCCTTCCAGTCCCAAGGCAGCCCTCAGAAGGGCCCAGAGC TCTCCCTGGCTAGTGTCCATGTGCCCCTGGAATCGATCAAGCCTAGCAGTGCCCTTCCTGTGACAGCCTA TGATAAAAATGGCTTCCGCATCCTTTTCCACTTTGCTAAGGAGTGTCCGCCAGGACGGCCTGATGTGCTG GTGGTGGTGGTTTCCATGCTGAATACAGCTCCCTTGCCAGTCAAGAGTATAGTGCTACAGGCCGCAGTGC CCAAGTCAATGAAAGTGAAGTTGCAGCCACCCTCTGGGACAGAACTTTCTCCATTTAGTCCCATCCAGCC ACCTGCAGCCATCACCCAGGTCATGCTGCTGGCCAATCCAATGAAGGAAAAGGTACGGCTTCGGTATAAA TTGACCTTTGCTCTGGGGGAGCAGCTGAGCACAGAGCTGGGTGAAGTGGACCAGTTTCCTCCTGTGGAGC AGTGGGGGAACCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200582 representing NM_001108304
Red=Cloning site Green=Tags(s) MAEAEGESLESWLNKATNPSNRQEDWEYIIGFCDQINKELEGPQIAVRLLAHKIQSPQEWEAVQALTVLE ACMKNCGRRLHNEVGKFRFLNELIKVVSPKYLGDRVSEKVKAKVIELLFSWTLALPEEAKIKDAYHMLKR QGIVQSDPPIPMDRTLIPSPPPRPKNPVFDDEEKSKLLAKLLRSKNPDDLQEANQLIKSMVKEDEARIQK VTKRLHTLEEVNNNVKLLHEMLLHYSQEFSSEADKELMKELFDRCENKRRTLFKLASETEDNDNSLGDIL QASDNLSRVINSYKTIIEGQIINGEVTTSTVPDSEGNSHCGNQGALIDLAELDTPSSSSPVLAPAPAPPT SGIPILPPPPQTSGPPRSRSSSQAEAPSGPDSTNNALSLLDEELLCLAPVVPASAVTHSTGSFTFSSGPA PALVPKAEPEGPEYPSSSISHRLDALDQLLEEAKVTSGLVKPVSCFSPGPTASPLLPASTPARPLLPFST GPGSPLFQSPAFQSQGSPQKGPELSLASVHVPLESIKPSSALPVTAYDKNGFRILFHFAKECPPGRPDVL VVVVSMLNTAPLPVKSIVLQAAVPKSMKVKLQPPSGTELSPFSPIQPPAAITQVMLLANPMKEKVRLRYK LTFALGEQLSTELGEVDQFPPVEQWGNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001108304 |
| ORF Size | 1974 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001108304.1, NP_001101774.1 |
| RefSeq Size | 4565 bp |
| RefSeq ORF | 1977 bp |
| Locus ID | 360658 |
| MW | 71.7 kDa |
| Gene Summary | Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Involved in BACE1 transport and sorting as well as regulation of BACE1 protein levels. Regulates retrograde transport of BACE1 from endosomes to the trans-Golgi network via interaction through the VHS motif and dependent of BACE1 phosphorylation. Modulates BACE1 protein levels intedepently of the interaction between VHS domain and DXXLL motif through recognition of ubiquitination (By similarity). Key player in a novel DXXLL-mediated endosomal sorting machinery to the recycling pathway that targets NTRK1 to the plasma membrane (PubMed:26446845).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200582 | Gga3 (untagged ORF) - Rat golgi associated, gamma adaptin ear containing, ARF binding protein 3 (Gga3), (10 ug) |
CNY 8,460.00 |
|
| RR200582L3 | Lenti ORF clone of Gga3 (Myc-DDK-tagged ORF) - Rat golgi associated, gamma adaptin ear containing, ARF binding protein 3 (Gga3), (10 ug) |
CNY 10,260.00 |
|
| RR200582L4 | Lenti ORF clone of Gga3 (mGFP-tagged ORF) - Rat golgi associated, gamma adaptin ear containing, ARF binding protein 3 (Gga3), (10 ug) |
CNY 11,310.00 |
