Raf1 (NM_012639) Rat Untagged Clone

CAT#: RN200564

Raf1 (untagged ORF) - Rat v-raf-leukemia viral oncogene 1 (Raf1), (10 ug)



  "NM_012639" in other vectors (3)

CNY 8,360.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Anti-Raf-1 (Ser301) Antibody (Phospho-Specific)
    • 100 ul

CNY 5,643.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN200564 representing NM_012639
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCACATACAGGGAGCTTGGAAGACGATCAGCAATGGTTTCGGACTCAAAGATGCTGTGTTTGATG
GCTCCAGTTGCATCTCCCCTACAATTGTTCAGCAGTTTGGCTATCAGCGTCGGGCCTCTGATGATGGCAA
ACTCACGGATTCTTCTAAGACAAGCAATACTATCCGGGTTTTCTTGCCGAATAAGCAAAGGACTGTGGTC
AATGTGCGGAATGGGATGAGCTTGCACGACTGCCTTATGAAAGCTCTGAAGGTTAGAGGCCTGCAGCCAG
AGTGCTGTGCAGTGTTCAGACTTCTTCAGGAGCACAAAGGTAAGAAAGCACGCTTAGATTGGAACACCGA
CGCCGCCTCTCTGATTGGAGAAGAACTGCAAGTGGATTTTTTGGATCACGTTCCACTCACAACTCACAAC
TTTGCTCGGAAAACGTTCCTGAAGCTTGCATTCTGTGACATCTGTCAAAAGTTCCTGCTAAATGGATTTC
GATGTCAGACTTGTGGCTACAAGTTTCATGAGCACTGTAGCACCAAAGTACCTACTATGTGTGTGGACTG
GAGTAATATCAGACAGCTCTTGCTGTTTCCAAATTCCACTGCAAGTGACAGTGGAGTCCCAGCACCACCC
TCTTTCACAATGCGTCGGATGCGAGAATCTGTTTCCCGGATGCCTGCTAGTTCCCAGCACAGATACTCCA
CACCCCATGCCTTCACTTTCAACACCTCCAGCCCTTCCTCTGAAGGTTCCCTGTCCCAGAGGCAGAGGTC
AACGTCCACTCCCAATGTCCACATGGTCAGCACCACCCTGCCTGTGGACAGCAGGATGATTGAGGATGCA
ATTCGAAGTCACAGTGAATCAGCCTCACCTTCAGCCCTGTCCAGCAGCCCCAACAACCTGAGCCCAACAG
GCTGGTCACAGCCCAAAACCCCTGTGCCAGCACAAAGAGAGAGGGCGCCAGGATCTGGGACCCAGGAAAA
AAACAAAATTAGGCCTCGTGGGCAGAGAGATTCAAGTTATTACTGGGAAATAGAAGCCAGTGAGGTGATG
CTGTCTACTCGGATTGGCTCGGGCTCCTTTGGCACTGTGTACAAGGGCAAGTGGCATGGAGATGTTGCAG
TAAAGATCCTAAAGGTGGTTGACCCAACTCCAGAGCAACTTCAGGCCTTCAGGAACGAGGTGGCTGTTTT
GCGCAAAACACGGCATGTTAATATCCTGCTGTTCATGGGGTACATGACAAAGGACAACCTGGCGATTGTG
ACCCAGTGGTGTGAAGGCAGCAGTCTCTACAAACACCTGCATGTCCAGGAGACCAAATTCCAGATGTTCC
AGCTAATTGACATTGCCCGGCAGACAGCTCAGGGAATGGACTATTTACATGCAAAGAACATCATCCACAG
AGACATGAAATCCAACAATATATTTCTCCATGAAGGCCTCACGGTGAAAATCGGAGATTTTGGTTTGGCA
ACAGTGAAGTCGCGCTGGAGTGGTTCTCAGCAGGTTGAACAGCCCACTGGCTCTGTGCTGTGGATGGCCC
CAGAAGTAATCCGAATGCAGGATAACAACCCGTTCAGCTTCCAGTCCGATGTCTACTCCTATGGCATTGT
GCTGTATGAGCTGATGACTGGGGAGCTTCCCTACTCCCACATCAACAACCGAGACCAGATCATCTTCATG
GTGGGCCGTGGGTACGCCTCCCCAGATCTTAGCAGGCTCTACAAGAACTGCCCCAAGGCAATGAAGAGGT
TGGTGGCTGACTGTGTGAAGAAAGTCAAAGAAGAAAGGCCTTTGTTTCCTCAGATCCTGTCTTCCATTGA
GCTGCTTCAGCACTCTCTGCCGAAAATCAACAGGAGCGCCTCTGAGCCTTCCCTGCATCGGGCAGCTCAC
ACTGAGGACATCAATGCTTGTACGCTGACCACATCCCCAAGGCTACCAGTCTTCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_012639
Insert Size 1947 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_012639.2, NP_036771.1
RefSeq Size 2970 bp
RefSeq ORF 1947 bp
Locus ID 24703
UniProt ID P11345
Gene Summary acts as a mitogenic protein kinase; mutant forms may play a role in transformation [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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