Grm1 (NM_017011) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Gprc1a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200513 representing NM_017011 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCGGCTCCTCTTGATTTTCTTCCCAATGATCTTTTTGGAGATGTCCATTTTGCCCAGGATGCCTG ACAGAAAAGTATTGCTGGCAGGTGCCTCGTCCCAGCGCTCCGTGGCGAGAATGGACGGAGATGTCATCAT CGGAGCCCTCTTCTCAGTCCATCACCAGCCTCCAGCCGAGAAGGTACCCGAAAGGAAGTGTGGGGAGATC AGGGAACAGTATGGTATCCAGAGGGTGGAGGCCATGTTCCACACGTTGGATAAGATTAACGCGGACCCGG TGCTCCTGCCCAACATCACTCTGGGCAGTGAGATCCGGGACTCCTGCTGGCACTCTTCAGTGGCTCTCGA ACAGAGCATCGAATTCATCAGAGACTCCCTGATTTCCATCCGAGATGAGAAGGATGGGCTGAACCGATGC CTGCCTGATGGCCAGACCCTGCCCCCTGGCAGGACTAAGAAGCCTATTGCTGGAGTGATCGGCCCTGGCT CCAGCTCTGTGGCCATTCAAGTCCAGAATCTTCTCCAGCTGTTCGACATCCCACAGATCGCCTATTCTGC CACAAGCATAGACCTGAGTGACAAAACTTTGTACAAATACTTCCTGAGGGTGGTCCCTTCTGACACTTTG CAGGCAAGGGCGATGCTCGACATAGTCAAGCGTTACAACTGGACCTATGTCTCAGCAGTCCACACAGAAG GGAATTACGGCGAGAGTGGAATGGATGCTTTCAAAGAACTGGCTGCCCAGGAAGGCCTCTGCATCGCACA CTCGGACAAAATCTACAGCAATGCTGGCGAGAAGAGCTTTGACCGGCTCCTGCGTAAACTCCGGGAGCGG CTTCCCAAGGCCAGGGTTGTGGTCTGCTTCTGCGAGGGCATGACAGTGCGGGGCTTACTGAGTGCCATGC GCCGCCTGGGCGTCGTGGGCGAGTTCTCACTCATTGGAAGTGATGGATGGGCAGACAGAGATGAAGTCAT CGAAGGCTATGAGGTGGAAGCCAACGGAGGGATCACAATAAAGCTTCAGTCTCCAGAGGTCAGGTCATTT GATGACTACTTCCTGAAGCTGAGGCTGGACACCAACACAAGGAATCCTTGGTTCCCTGAGTTCTGGCAAC ATCGCTTCCAGTGTCGCCTACCTGGACACCTCTTGGAAAACCCCAACTTTAAGAAAGTGTGCACAGGAAA TGAAAGCTTGGAAGAAAACTATGTCCAGGACAGCAAAATGGGATTTGTCATCAATGCCATCTATGCCATG GCACATGGGCTGCAGAACATGCACCATGCTCTGTGTCCCGGCCATGTGGGCCTGTGTGATGCTATGAAAC CCATTGATGGCAGGAAGCTCCTGGATTTCCTCATCAAATCCTCTTTTGTCGGAGTGTCTGGAGAGGAGGT GTGGTTCGATGAGAAGGGGGATGCTCCCGGAAGGTATGACATTATGAATCTGCAGTACACAGAAGCTAAT CGCTATGACTATGTCCACGTGGGGACCTGGCATGAAGGAGTGCTGAATATTGATGATTACAAAATCCAGA TGAACAAAAGCGGAATGGTACGATCTGTGTGCAGTGAGCCTTGCTTAAAGGGTCAGATTAAGGTCATACG GAAAGGAGAAGTGAGCTGCTGCTGGATCTGCACGGCCTGCAAAGAGAATGAGTTTGTGCAGGACGAGTTC ACCTGCAGAGCCTGTGACCTGGGGTGGTGGCCCAACGCAGAGCTCACAGGCTGTGAGCCCATTCCTGTCC GTTATCTTGAGTGGAGTGACATAGAATCTATCATAGCCATCGCCTTTTCTTGCCTGGGCATCCTCGTGAC GCTGTTTGTCACCCTCATCTTCGTTCTGTACCGGGACACACCCGTGGTCAAATCCTCCAGTAGGGAGCTC TGCTATATCATTCTGGCTGGTATTTTCCTCGGCTATGTGTGCCCTTTCACCCTCATCGCCAAACCTACTA CCACATCCTGCTACCTCCAGCGCCTCCTAGTTGGCCTCTCTTCTGCCATGTGCTACTCTGCTTTAGTGAC CAAAACCAATCGTATTGCACGCATCCTGGCTGGCAGCAAGAAGAAGATCTGCACCCGGAAGCCCAGATTC ATGAGCGCTTGGGCCCAAGTGATCATAGCCTCCATTCTGATTAGTGTACAGCTAACACTAGTGGTGACCT TGATCATCATGGAGCCTCCCATGCCCATTTTGTCCTACCCGAGTATCAAGGAAGTCTACCTTATCTGCAA TACCAGCAACCTGGGTGTAGTGGCCCCTGTGGGTTACAATGGACTCCTCATCATGAGCTGTACCTACTAT GCCTTCAAGACCCGCAACGTGCCGGCCAACTTCAATGAGGCTAAATACATCGCCTTCACCATGTACACTA CCTGCATCATCTGGCTGGCTTTCGTTCCCATTTACTTTGGGAGCAACTACAAGATCATCACTACCTGCTT CGCGGTGAGCCTCAGTGTGACGGTGGCCCTGGGGTGCATGTTTACTCCGAAGATGTACATCATCATTGCC AAACCTGAGAGGAACGTCCGCAGTGCCTTCACGACCTCTGATGTTGTCCGCATGCACGTCGGTGATGGCA AACTGCCGTGCCGCTCCAACACCTTCCTCAACATTTTCCGGAGAAAGAAGCCCGGGGCAGGGAATGCCAA TTCTAACGGCAAGTCTGTGTCATGGTCTGAACCAGGTGGAAGACAGGCGCCCAAGGGACAGCACGTGTGG CAGCGCCTCTCTGTGCACGTGAAGACCAACGAGACGGCCTGTAACCAAACAGCCGTAATCAAACCCCTCA CTAAAAGTTACCAAGGCTCTGGCAAGAGCCTGACCTTTTCAGATGCCAGCACCAAGACCCTTTACAATGT GGAAGAAGAGGACAATACCCCTTCTGCTCACTTCAGCCCTCCCAGCAGCCCTTCTATGGTGGTGCACCGA CGCGGGCCACCCGTGGCCACCACACCACCTCTGCCACCCCATCTGACCGCAGAAGAGACCCCCCTGTTCC TGGCTGATTCCGTCATCCCCAAGGGCTTGCCTCCTCCTCTCCCGCAGCAGCAGCCACAGCAGCCGCCCCC TCAGCAGCCCCCGCAGCAGCCCAAGTCCCTGATGGACCAGCTGCAAGGCGTAGTCACCAACTTCGGTTCG GGGATTCCAGATTTCCATGCGGTGCTGGCAGGCCCGGGGACACCAGGAAACAGCCTGCGCTCTCTGTACC CGCCCCCGCCTCCGCCGCAACACCTGCAGATGCTGCCCCTGCACCTGAGCACCTTCCAGGAGGAGTCCAT CTCCCCTCCTGGGGAGGACATCGATGATGACAGTGAGAGATTCAAGCTCCTGCAGGAGTTCGTGTACGAG CGCGAAGGGAACACCGAAGAAGATGAATTGGAAGAGGAGGAGGACCTGCCCACAGCCAGCAAGCTGACCC CTGAGGATTCTCCTGCCCTGACGCCTCCTTCTCCTTTCCGAGATTCCGTGGCCTCTGGCAGCTCAGTGCC CAGTTCCCCCGTATCTGAGTCGGTCCTCTGCACCCCTCCAAATGTAACCTACGCCTCTGTCATTCTGAGG GACTACAAGCAAAGCTCTTCCACCCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_017011
Insert Size	3600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017011.1, NP_058707.1
RefSeq Size	6820 bp
RefSeq ORF	3600 bp
Locus ID	24414
UniProt ID	P23385
Gene Summary	may play a role in synaptic transmission; may modulate perception of pain in arthritis [RGD, Feb 2006] Transcript Variant: This variant (1) encodes the longer isoform (alpha).

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