Dmd (NM_001005244) Rat Untagged Clone

CAT#: RN200462

Dmd (untagged ORF) - Rat dystrophin, muscular dystrophy (Dmd), transcript variant Dp71ab, (10 ug)



  "NM_001005244" in other vectors (3)

CNY 7,980.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms DNADMD1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN200462 representing NM_001005244
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGAACACCTCAAAGGCCACGAGACCCAAACAACTTGTTGGGACCATCCCAAAATGACAGAGCTCT
ACCAGTCTTTAGCTGACCTGAATAATGTCAGGTTCTCCGCTTATAGGACTGCCATGAAGCTCAGAAGACT
CCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGACGCCTTGGACCAGCACAACCTC
AAGCAAAATGATCAGCCCATGGATATCCTGCAGATAATTAACTGTTTGACTACTATTTATGACCGTCTGG
AGCAAGAGCACAACAATTTGGTCAATGTCCCTCTCTGTGTGGATATGTGTCTCAACTGGCTTCTCAATGT
TTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCTCTGTGTAAA
GCACACTTGGAAGACAAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACCGGCTTTTGTGACCAGC
GTAGACTGGGTCTTCTTCTGCATGATTCTATTCAAATCCCAAGACAGTTGGGTGAAGTTGCCTCCTTTGG
GGGCAGTAACATTGAGCCGAGTGTCAGGAGCTGTTTCCAGTTTGCCAATAATAAACCTGAGATTGAAGCC
GCTCTCTTCCTTGACTGGATGCGTCTGGAACCCCAGTCTATGGTGTGGCTGCCGGTCTTGCACAGAGTGG
CTGCTGCTGAAACAGCCAAGCATCAGGCCAAGTGTAACATCTGTAAGGAGTGTCCAATCATTGGATTCAG
GTACAGAAGCCTGAAACACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGGCCGAGTTGCTAAG
GGCCATAAAATGCACTACCCCATGGTAGAGTACTGCACTCCGACTACATCTGGAGAAGATGTTCGTGACT
TTGCCAAGGTACTGAAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCT
TCCAGTGCAGACTGTGTTAGAGGGGGACAACATGGAAACGCCTGCCTCGTCCCCCCAGCTTTCACACGAT
GATACTCATTCACGCATTGAGCATTATGCTAGCAGGCTAGCAGAAATGGAAAACAGCAATGGATCTTATC
TAAATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAG
TTTGAACCAGGACTCCCCCCTGAGCCAGCCTCGTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAG
GAAAGAGGGGAGCTAGAGAGAATCCTAGCAGATCTTGAGGAAGAAAACAGAAATCTGCAAGCAGAATATG
ATCGTCTGAAGCAGCAGCATGAGCATAAAGGCCTGTCCCCACTGCCGTCTCCTCCTGAGATGATGCCCAC
CTCTCCCCAGAGTCCCAGGGATGCTGAACTCATTGCTGAGGCCAAGCTACTGCGCCAACACAAAGGACGT
CTGGAAGCCAGGATGCAAATCCTGGAAGACCACAATAAACAACTGGAGTCTCAGTTACATAGACTGAGGC
AGCTCCTGGAGCAACCCCAGGCTGAGGCCAAGGTGAATGGCACCACGGTGTCCTCTCCTTCTACCTCGCT
GCAGAGGTCAGATAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCAGAATCTATGGGT
GAGGAAGATCTTCTGAGTCCTCCCCAGGACACAAGCACAGGGTTAGAAGAAGTGATGGAGCAACTCAACA
ACTCCTTCCCTAGTTCAAGAGGACACAATGTAGGAAGCCTTTTCCACATGGCAGATGATTTGGGCAGAGC
GATGGAGTCCTTAGTTTCAGTCATGATAGATGAAGAAGGAGCAGAATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001005244
Insert Size 1869 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001005244.2, NP_001005244.1
RefSeq Size 1990 bp
RefSeq ORF 1869 bp
Locus ID 24907
Gene Summary a vital component of a muscle sarcolemma membrane-spanning complex that connects cytoskeleton to basal lamina; plays a role in retinal neurotransmission; mutations cause Duchenne muscular dystrophy [RGD, Feb 2006]
Transcript Variant: This variant (Dp71ab) lacks an exon in the 3' coding region, resulting in a frameshift, as compared to variant Dp71a. The encoded isoform Dp71ab has a longer and distinct C-terminus, as compared to isoform Dp71a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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