Itsn1 (NM_019227) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	EHSH1; Itsn
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200381 representing NM_019227 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTTTCCGACACCTTTTGGTGGGAGCCTGGACATCTGGGCCATAACCGTGGAGGAAAGAGCCA AGCATGACCAGCAGTTCCAGAGTTTGAAGCCGATATCTGGATTCATCACTGGTGATCAAGCAAGGAACTT CTTTTTCCAATCTGGGTTACCTCAGCCCGTCTTAGCACAAATATGGGCGCTGGCTGACATGAATAAGGAC GGAAGGATGGATCAGGTGGAGTTTTCCATAGCCATGAAGCTCATCAAACTGAAGCTGCAAGGATATCAGC TCCCCCCCGCACTTCCCCCTGTCATGAAGCAGCAGCCAGCGGCCATCTCTAGTGCACCAGCGTTTGGTAT AGGAGGGATGGCTGGAATGCCACCACTGACAGCTGTTGCTCCCGTGCCAATGGGCTCCATCCCAGTTGTT GGAATGTCTCCGCCCTTAGTATCTTCTGTCCCTCAAGCAGCAGTGCCTCCCCTGGCTAACGGGGCCCCTC CTGTCATACAGCCTCTGCCTGCATTTGCTCATCCTGCAGCCACATTGCCAAAGAGTTCTTCCTTCAGCAG ATCTGGTCCAGGGTCACAATTAAACACTAAACTACAGAAGGCACAATCATTTGATGTAGCCAGCGCCCCT GCAGCGGCAGAATGGGCTGTGCCTCAGTCGTCAAGACTGAAATACAGGCAGTTATTCAACAGTCACGACA AGACCATGAGTGGACACTTAACAGGTCCCCAGGCAAGAACTATTCTCATGCAATCAAGTTTACCCCAGGC TCAGCTGGCTTCAATCTGGAATCTTTCCGACATTGATCAAGATGGAAAGCTCACCGCAGAAGAATTCATC CTAGCGATGCACCTGATTGATGTTGCTATGTCTGGTCAGCCACTGCCGCCTGTCCTGCCTCCAGAATACA TCCCTCCTTCCTTTAGAAGAGTTCGCTCTGGCAGTGGGATGTCCGTCATAAGCTCTTCGTCTGCAGACCA GCGGCTGCCCGAGGAGCCATCGTCAGAGGATGAGCAGCAGGTGGAAAAGAAGCTGCCTGTGACATTTGAA GATAAGAAGCGGGAGAACTTCGAGCGAGGCAACCTGGAGCTGGAGAAGCGCAGGCAGGCCCTCCTGGAGC AGCAGCGCAAGGAGCAGGAGCGGCTGGCTCAGCTGGAGCGCGCCGAGCAAGAGAGGAAAGAGCGGGAGCG CCAGGAACAGGAGCGCAAGAGACAGCTAGAGCTGGAGAAGCAGCTAGAAAAGCAGCGGGAGCTGGAGAGG CAGAGAGAGGAGGAGAGAAGGAAGGAGATCGAGAGGCGGGAGGCAGCAAAACGGGAGCTCGAACGGCAGC GACAGCTTGAATGGGAACGGAACCGGAGACAGGAACTCCTGACTCAGAGGAACAAGGACCAGGAGGGCAT CGTGGTTCTGAAGGCGAGGAGGAAGACTCTGGAGTTTGAATTAGAAGCTCTGAATGACAAAAAGCATCAG CTGGAAGGAAAACTTCAGGATATCAGGTGTCGACTGGCAACCCAGAGGCAAGAAATTGAGAGCACAAACA AGTCTAGAGAGCTGAGAATTGCGGAAATCACCCACTTACAGCAACAGTTGCAGGAATCTCAGCAGATGCT TGGAAGACTTATTCCAGAGAAGCAGATACTCAGTGACCAGTTAAAACAAGTCCAGCAGAACAGCTTGCAT AGAGATTCGCTTCTTACCCTCAAAAGAGCCTTGGAAGCAAAGGAACTGGCCCGGCAGCAGCTTCGAGAGC AGCTGGACGAGGTGGAGAAAGAGACCAGGTCAAAGCTGCAGGAGATTGATGTTTTCAACAACCAGCTGAA GGAACTGAGAGAGATACACAGTAAACAGCAGCTCCAGAAGCAGAGGTCCATCGAGGCGGAGAGGCTAAAG CAGAAAGAGCAGGAGAGGAAGAGCCTGGAGTTGGAGAAGCAAAAGGAAGAAGGTCAGAGACGAGTTCAGG AAAGGGACAAGCAATGGCAGGAGCATGTCCAGCAGGAGGAGCAGCAGCGCCCTCGGAAACCCCACGAGGA GGACAAACTGAAAAGGGAAGACAGTGTCAAGAAGAAGGAGGCGGAAGAGAGAGCCAAGCCGGAAGTGCAA GACAAGCAGAGTCGGCTTTTCCATCCACATCAAGAGCCAGCTAAGCCGGCGCAGGCACCCTGGCCCACGA CAGAGAAAGGTCCGCTTACAATCTCTGCACAGGAGAGTGCCAAAGTGGTGTATTACCGAGCGCTGTACCC CTTTGAGTCCAGAAGTCATGACGAGATCACCATCCAGCCAGGAGATATAGTCATGGTTAAAGGGGAATGG GTGGATGAAAGCCAGACCGGAGAGCCAGGATGGCTTGGAGGAGAACCGAAGGGGAAGACAGGATGGTTCC CTGCAAACTATGCAGAGAAGATTCCAGAAAATGAGATTCCCACTCCAGCCAAACCAGTGACCGATCTGAC ATCTGCCCCTGCCCCCAAACTGGCTCTGCGTGAGACCCCTGCTCCTTTGCCAGTGACCTCTTCTGAGCCC TCCACAACCCCCAACAACTGGGCAGACTTCAGTTCCACGTGGCCCAGCAGCACAAATGAGAAACCAGAAA CGGACAACTGGGACACGTGGGCGGCTCAGCCTTCTCTGACTGTACCCAGTGCTGGCCAGTTAAGGCAGAG GTCGGCCTTCACACCAGCCACAGCCACTGGCTCCTCCCCTTCTCCCGTCCTGGGCCAGGGTGAAAAGGTG GAAGGGCTCCAAGCACAAGCCCTGTATCCCTGGAGAGCCAAAAAAGACAACCACTTAAATTTTAACAAAA GTGATGTCATCACCGTTCTGGAACAGCAAGACATGTGGTGGTTTGGAGAAGTTCAAGGTCAGAAGGGTTG GTTCCCCAAGTCTTACGTGAAACTCATTTCAGGGCCCGTAAGGAAATCCACAAGCATCGATACTGGCCCT ACGGAAGCTCCCTCTAGTCTAAAGAGAGTGGCTTCCCCAGCCGCCAAGCCAGCCATTCCTGGAGAAGAAT TTGTTGCTATGTACACTTACGAGAGTTCTGAGCATGGAGATTTAACCTTTCAGCAAGGGCATGTGATTGT GGTTACCAAGAAGGATGGTGACTGGTGGACGGGCACAGTGGGCGAGACGTCCGGAGTCTTCCCTTCTAAC TATGTGAGGCTTAAAGACTCAGAGGGCTCTGGAACTGCGGGGAAAACAGGGAGTTTAGAAAAAAAACCTG AAATCGCCCAGGTCATTGCTTCCTACACTGCTACGGGTCCTGAACAGCTCACCCTGGCTCCTGGTCAGCT GATTTTGATCCGAAAAAAGAACCCCGGTGGATGGTGGGAAGGAGAACTACAAGCTCGAGGGAAAAAGCGC CAGATAGGGTGGTTCCCAGCAAATTATGTCAAACTTCTAAGCCCTGGAACAAGCAAAATCACCCCAACTG AGCTACCCAAGACCGCAGTGCAGCCAGCAGTGTGCCAGGTGATCGGGATGTACGATTACACCGCGCAGAA TGACGATGAGCTAGCCTTCAGCAAAGGCCAGATCATCAACGTCCTCAGCAAGGAGGACCCGGACTGGTGG AAAGGAGAAGTCAGTGGGCAGGTTGGGCTCTTCCCGTCCAATTATGTGAAGCTGACCACAGACATGGACC CCAGCCAGCAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019227
Insert Size	3654 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019227.1, NP_062100.1
RefSeq Size	4025 bp
RefSeq ORF	3654 bp
Locus ID	29491
UniProt ID	Q9WVE9
Gene Summary	binds to dynamin and SNAP-25; may be involved in exocytosis [RGD, Feb 2006] Transcript Variant: This variant (2) lacks alternate exons in the 3' coding region and 3' UTR, compared to variant 1, resulting in a novel 3' coding region and 3' UTR. It encodes the shorter isoform (b), compared to isoform a.

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