Epb41l1 (NM_021681) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	4.1N; Epb4.1l1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200334 representing NM_021681 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACAGAGACAGGCCCAGATTCTGAGGTGAAGAAGGCTCAGGAGGAGACTCCACAGCAGCCAGAGG CTGCTGCAGCTGTGACCACCCCGGTGACCCCCGCAGGCCACAGCCACCCAGAGACCAATTCCAATGAGAA GCATCTGACCCAGCAGGACACAAGGCCTGCTGAACAGAGCCTAGATATGGAGGAAAAGGATTACTGTGAG GCTGATGGCCTGTCGGAGAGGACTACGCCCAGTAAGGCCCAGAAGTCACCCCAGAAGATTGCCAAGAAGT TCAAGAGTGCCACCTGCCGCGTCACCCTGCTTGATGCCTCTGAGTACGAGTGTGAGGTGGAGAAGCACGG CCGGGGCCAGGTGCTGTTTGACCTGGTCTGTGAGCACCTTAACCTCCTCGAGAAGGACTACTTCGGTCTG ACCTTCTGCGATGCCGACAGCCAGAAGAATTGGCTGGACCCCTCCAAGGAAATCAAGAAACAGATCCGGA GTAGCCCCTGGAATTTTGCCTTCACAGTGAAGTTCTACCCTCCTGACCCAGCCCAGCTGACAGAAGACAT CACAAGGTACTACCTGTGCCTGCAGCTGAGGGCAGACATCATCACGGGCCGCCTGCCTTGCTCCTTCGTC ACGCACGCCCTGCTGGGCTCCTACGCTGTGCAGGCTGAACTGGGTGACCATGATACCGAGGAGCACGTGG GCAACTATGTCAGCGAGCTCCGCTTTGCCCCAAACCAGACCCGGGAGCTGGAGGAGAGGATCATGGAGCT GCACAAGACATACAGGGGCATGACCCCAGGCGAGGCAGAGATCCACTTCCTGGAGAATGCCAAGAAGCTC TCCATGTATGGAGTTGATCTGCACCATGCTAAGGACTCTGAGGGCATCGATATCATGCTAGGTGTCTGTG CCAATGGCCTGCTCATCTACCGGGACCGCCTGAGAATCAACCGTTTTGCCTGGCCCAAGATTCTCAAGAT CTCCTACAAGAGGAGTAACTTCTACATCAAGATCCGTCCTGGGGAGTATGAGCAGTTCGAGAGCACCATC GGCTTCAAGCTCCCTAACCACCGCTCAGCCAAGCGGCTGTGGAAGGTCTGCATTGAGCACCATACATTCT TCCGGCTGGTGTCTCCAGAGCCCCCACCCAAGGGCTTTCTGGTGATGGGCTCCAAGTTCCGGTACAGTGG GAGGACCCAGGCACAGACTCGCCAAGCCAGTGCCCTTATCGACAGGCCCGCACCCTTCTTTGAGCGTTCT TCCAGCAAACGATACACCATGTCCCGGAGCCTTGATGGAGCTGAGTTCTCCCGCCCTGCCTCAGTCAGTG AGAATCACGATGCAGGGCCTGACGGCGACAAGCGGGAAGATGATGCCGAGTCAGGGGGACGTCGATCAGA GGCCGAAGAAGGAGAGGTCAGGACCCCCACTAAGATCAAGGAGCTGAAGCCGGAGCAGGAAACCACGCCA AGACACAAGCAGGAGTTCCTGGACAAGCCAGAGGACGTCTTGTTGAAGCACCAGGCCAGCATCAATGAAC TCAAGAGGACCCTGAAGGAGCCCAATAGCAAACTGATCCACCGGGATCGAGACTGGGAGCGGGAGCGCAG GCTGCCCTCATCACCCGCCTCCCCCTCTCCCAAGGGCACCCCTGAGAAAGCCAGTGAGGCCCAGAGGATC CAGGACACTTCTCGGCAGGACTTGGTACCTGGAACAGCAACAGGCTTGGAGATGTTCACTCAGAAAAGCC TCGCAGCATCCCCTGAGGGCTCAGAGCATTGGGTATTTATAGAAAGAGTGTACACTAGGCCAGAAGACCT CGGCCTCCTAACAGTGGCCGCCACACAACGGAAAGAAAGTGGATCAAGCCTCAGTGGGATCCTTGCTGAC GGCAGACTCTCCAAGGTAGACATTCTGGTGGACAAGTTCAAAGTCGAAGTGGCCACGGAAGAAACAGTGA GAACCAGAAGAACAAGTACTCAACAGCAGGGCAAGATGCTTGCAAGTCCAGAAGACTTTGAGACCGTGAG AGAGGAAGACCGGGGAGGTCCCGGAGAGGCCACATCAACTGACAAGCTTCCCGAGGGCTCCAGGCTCAAG CTCAGGAACCACGGAGTCTCAAATGGTCAACTAGAGAGCCAAGTAGAATGGGGACTGGAGAGGCCCCAGA CCTGGGGAAGACCAACAGCTGCAGGGCTTGGCCCAGTGCAGGGAGAGGTTCTTTCTCCTACATCAGACAA GGGAGGACTCCAGTCCTTCCTGTTGGATCCAGCCCAAGCCGAAGCCAGAGCTGACTCAAGCGATGAGACT GACACCTCCTTCGCAGAAAGGAGCTTCTGTCTGCACTATGGAGAGAAAGACTCTGAAGACCAGCTTCTCG CCCCACCCTCAGAGCACAGAGAAGAGCACCCAGATGCCCTTCCTGGAGATGGGACCTGGTTGGAGCTGGC AGGAGTGCACACAGAGAACTGGGAGCTGAAGTCCTCAGACCCAAGAGCTTCTGCCCCAGGTTCCAGCCAG CATAAAGACCAAGCCCATAAGGCTCCCTCAGCAGAAGAAGCCTGGACTCCCAGGGACCATGGAAGACCTG ATGACCCTCAGGGAGCTGCTGTCGGTCAGACTTTTGAAGAGGTCTGGGAAAACACACAGCAGAGGCTAGA AGGAGAGTTGGTCCAGCCACTGGCCAGTGTAGCTGAAGATGAGGTCCCCACAAATATGGACTGGATGGGA AAGACTGAGAAAAGTCCCCCAGCAAGAAGGAAGAAGAGCCCCCCTGGTAGAGGAGGAGGGGTGCACCTAG ACGCCCAGGCTTGTGCCCTTCTGAGGACCATCCCTCCTTGTGTCAGGAAGCCAGCCAGGCCAGACCAAGG CAGCTTCCTGCCCAAAGAGAAGGGGACAGTTTCCCCTCCTGCAGTAGAACCTGAGACAGAGGACAGGGAG ACAGTCAGCCCCCTGCCAGTCTCCTCTGGTCACACTGAGGTTCTGGCAGCTATGGAGGGCACCTCTCTAA GCCCACTACCTCCTGGGTCAAGGGGGCCTTCAGAGTCCAGGGAATTCTTTAGAGACCAGTTCCCTGTAGT TCTCAAATATATCCATCTTCCTGAAGAGAGCCCAGTACCCAAGGACCCACACGGTGACCGCAAAGCACCT CCAGTTGCCAGCAAGAAGCCCAGGTTTGTCCCTGAAGGAAGTGAGGAACCTGTACTCCTGGGTGAATTGA TGTTCCCTTCAGGAAAGCAGGAGACTTCACTCCAGGACTGGGACCAAGGGGATTCCCAGGAAGATATTAG CAAGACCTCAGTGGCTAACAAAATTCGGATATTTGAGACCCATGGAGCTGAGGCTTGCCGAGCCAGTCAG GGTGAAATGAGGAGCCTTCCACATGAGCTGCCTTCAGGAGCTTCCCCAGGACAGGTAGAACAGCAAGATA ATCTTCTAGACTTGGGCTTTGTCCAACTCCAGCCCCCTGGGGACTTGGCCACCCCAACTACCTACCACCA GGAAAGATGCACAGACCCAGAGCTCGTGTCCCCTGATTCAGGCTGTGAAACCACGCTGGAGGAAGCTACT GGGAGCAAATCCGGAGACGCGGGCAGGGAAGACAAGAGCTCCTTCCGCCGCTTAGCGCCAAACACCCCTG GAAAGGGGGGGCGTCTGAGATTCGCCAGCCCTCCAGGCCCTCAGAGAGCAGGGCTGAGGGAGGGCTCGGA GGAGAAAGTCAAACCGCCACGCCCACGTGCCCCAGAGAGTGACACGGGAGATGAGGACCAGGACCAGGAG AGGGATGCGGTATTCTTGAAGGACAACCACCTGGCCATTGAACGCAAATGCTCCAGCATAACGGTCAGCT CCACGTCCAGCCTGGAAGCTGAGGTGGACTTCACGGTTATTGGTGACTACCACGGGGGCGCCTTTGAAGA CTTCTCCCGAAGCCTCCCTGAGCTCGATCGGGATAAAAGTGACTCTGAGACAGAGGGCCTGGTGTTCGCC AGGGACCTCAAGGGGCCCTCCAGCCAAGAGGATGAATCTGGGGGCATCGAGGACAGCCCGGATCGAGGGG CCTGTTCTACCCCAGAATTGCCCCAGTTTGAGTCTGTGAAAGCAGAAACCATGACCGTCAGCAGTCTGGC AATCAGGAAGAAGATCGAGCCAGAGGCCATGCTGCAAAGCAGAGTCTCCACTGCAGACAGCACCCAGGTT GATGGAGGTGCTCCAGCGGCGAAAGACTTCATGACAACTCCCCCCTGCATCACCACAGAGACCATCTCGA CCACTATGGAGAACAGTCTCAAGTCCGGGAAGGGGGCAGCTGCCATGATCCCAGGCCCACAGACGGTGGC CACGGAAATCCGTTCTCTTTCACCGATCATCGGGAAAGATGTCCTCACCAGCACGTACGGCGCCACCGCG GAAACCCTCTCAACCTCCACCACCACCCATGTCACCAAAACTGTGAAGGGAGGGTTTTCTGAGACAAGGA TCGAGAAGCGAATCATCATTACTGGGGATGAAGATGTCGATCAAGACCAGGCTCTGGCTTTGGCCATCAA GGAAGCCAAACTTCAGCATCCAGACATGCTGGTAACCAAAGCTGTTGTCTATAGAGAAACAGACCCATCC CCGGAAGAGAGGGACAAGAAGCCACAGGAATCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_021681
Insert Size	4656 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_021681.2, NP_067713.2
RefSeq Size	5813 bp
RefSeq ORF	4656 bp
Locus ID	59317
Gene Summary	human homolog interacts with D2 and D3 dopamine receptors; may play a role in dopamine receptor localization or stability [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (L). Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

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