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Becn1 (NM_053739) Rat Untagged Clone
CAT＃: RN200235
Becn1 (untagged ORF) - Rat beclin 1, autophagy related (Becn1), transcript variant 1, (10 ug)
CNY 5,800.00
| Cited in 9 publications. |
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Beclin1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200235 representing NM_053739
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGTCTAAGGCGTCCAGCAGCACCATGCAGGTGAGCTTCGTGTGCCAGCGCTGTAGCCAGCCTC TGAAACTGGACACGAGCTTCAAGATCCTGGACCGAGTGACCATTCAGGAACTCACAGCTCCATTACTTAC CACAGCCCAGGCGAAACCAGGAGAGAGCCAGGAGGAAGAGGCTAACTCAGGAGAGGAGCCATTTATTGAA ACTCGCCAGGATGGTGTCTCTCGAAGATTCATCCCCCCAGCCAGGATGATGTCTACAGAAAGTGCTAATA GCTTCACTCTGATCGGGGAGGCATCTGATGGTGGCACCATGGAGAACCTCAGCCGGAGACTCAAGGTCAC TGGAGACCTTTTTGACATCATGTCTGGCCAGACAGATGTGGATCACCCACTGTGTGAGGAATGCACAGAT ACTCTTTTAGACCAGCTGGACACTCAGCTCAATGTTACTGAAAACGAGTGTCAGAACTACAAACGCTGTT TGGAGATGTTGGAGCAAATGAATGAGGGCGACAGTGAACAGCTACAGAGGGAGCTGAAGGAGTTGGCCTT GGAGGAGGAGAGGCTGATCCAGGAGCTGGAAGATGTGGAAAAAAACCGAAAGGTGGTGGCAGAAAACCTG GAGAAGGTCCAGGCTGAGGCGGAGAGACTGGACCAGGAGGAAGCTCAGTACCAGCGAGAATATAGTGAAT TTAAAAGGCAGCAGCTGGAGCTGGATGATGAGCTCAAGAGTGTAGAGAACCAGATGCGCTATGCCCAGAT GCAGCTGGACAAGCTCAAGAAAACCAATGTCTTCAATGCGACCTTCCATATCTGGCACAGCGGACAATTT GGCACGATCAATAATTTCAGACTGGGTCGCTTGCCCAGTGCTCCTGTGGAATGGAATGAAATCAATGCTG CCTGGGGCCAGACAGTGTTGTTGCTCCATGCTTTGGCCAATAAGATGGGTCTGAAATTTCAGAGGTACCG ACTTGTTCCCTATGGAAATCACTCGTATCTGGAGTCCCTGACAGACAAATCTAAGGAGTTGCCGTTGTAC TGTTCTGGGGGTTTGCGGTTTTTCTGGGACAACAAGTTTGACCATGCAATGGTAGCTTTTCTGGACTGTG TGCAGCAGTTCAAAGAAGAGGTGGAAAAAGGAGAGACTCGATTTTGTCTTCCGTACAGGATGGACGTGGA GAAAGGCAAGATTGAAGACACTGGAGGCAGTGGCGGCTCCTATTCCATCAAAACCCAGTTTAACTCTGAG GAGCAGTGGACAAAGGCGCTCAAGTTCATGCTGACGAATCTCAAGTGGGGTCTTGCTTGGGTGTCCTCAC AGTTCTATAACAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_053739 |
| Insert Size | 1347 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_053739.2, NP_446191.1 |
| RefSeq Size | 2140 bp |
| RefSeq ORF | 1347 bp |
| Locus ID | 114558 |
| UniProt ID | Q91XJ1 |
| Gene Summary | mouse homolog is a haploinsufficient tumor suppressor gene; plays a role in early embryonic development [RGD, Feb 2006] |
Citations (9)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Cyclic GMP-AMP synthase contributes to epithelial homeostasis in intestinal inflammation via Beclin-1-mediated autophagy.
,null,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
,PubMed ID 35344224
[Becn1]
|
|
DEAD Box Protein 5 Inhibits Liver Tumorigenesis by Stimulating Autophagy via Interaction with p62/SQSTM1
,null,
Hepatology (Baltimore, Md.)
,PubMed ID 30281815
[Becn1]
|
|
HGK-sestrin 2 signaling-mediated autophagy contributes to antitumor efficacy of Tanshinone IIA in human osteosarcoma cells
,null,
Cell Death & Disease
,PubMed ID 30258193
[Becn1]
|
|
SPHK1 (sphingosine kinase 1) induces epithelial-mesenchymal transition by promoting the autophagy-linked lysosomal degradation of CDH1/E-cadherin in hepatoma cells
,null,
Autophagy
,PubMed ID 28521610
[Becn1]
|
|
Interferon Regulatory Factor-1 signaling regulates the switch between autophagy and apoptosis to determine breast cancer cell fate
,null,
Cancer research
,PubMed ID 25576084
[Becn1]
|
|
The epithelial polarity regulator LGALS9/galectin-9 induces fatal frustrated autophagy in KRAS mutant colon carcinoma that depends on elevated basal autophagic flux
,null,
Autophagy
,PubMed ID 26086204
[Becn1]
|
|
Cytosolic HMGB1 controls the cellular autophagy/apoptosis checkpoint during inflammation
,null,
The Journal of Clinical Investigation
,PubMed ID 25642769
[Becn1]
|
|
Autophagic program is regulated by miR-325
,null,
Cell Death and Differentiation
,PubMed ID 24531537
[Becn1]
|
|
Autophagy inhibition radiosensitizes in vitro , yet reduces radioresponses in vivo due to deficient immunogenic signalling
,null,
Cell Death and Differentiation
,PubMed ID 24037090
[Becn1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200235 | Becn1 (Myc-DDK-tagged ORF) - Rat beclin 1, autophagy related (Becn1), transcript variant 1, (10 ug) |
CNY 5,800.00 |
|
| RR200235L3 | Lenti ORF clone of Becn1 (Myc-DDK-tagged ORF) - Rat beclin 1, autophagy related (Becn1), transcript variant 1, (10 ug) |
CNY 7,600.00 |
|
| RR200235L4 | Lenti ORF clone of Becn1 (mGFP-tagged ORF) - Rat beclin 1, autophagy related (Becn1), transcript variant 1, (10 ug) |
CNY 8,460.00 |
