Pds5b (NM_001101805) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Aprin; AS3; CG008
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200111 representing NM_001101805 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCATTCAAAGACAAGAACCAACGATGGCAAAATTACTTACCCTCCTGGTGTCAAGGAGATCTCAG ATAAAATCTCTAAAGAGGAGATGGTGAGGCGGCTAAAGATGGTTGTAAAAACATTCATGGACATGGACCA GGACTCTGAAGAGGAAAAGGAACTGTATCTAAACCTAGCTTTACACCTTGCTTCTGACTTCTTCCTCAAG CATCCTGATAAAGATGTCCGTTTACTGGTGGCTTGCTGCCTTGCTGATATTTTCAGGATTTACGCTCCTG AGGCTCCTTACACATCTCCCGATAAACTAAAGGATATATTTATGTTTATAACAAGGCAATTGAAGGGACT GGAGGACACAAAGAGCCCTCAATTCAATAGGTATTTTTATTTACTTGAGAACATTGCATGGGTTAAATCA TATAACATATGCTTTGAGTTAGAAGATAGCAATGAAATCTTTACTCAACTATACAGAACATTATTCTCAG TTATAAACAATGGCCACAATCAGAAAGTTCACATGCACATGGTGGACCTTATGAGCTCTATCATCTGTGA AGGTGACACAGTATCGCAGGAGCTCCTAGATACAGTCTTAGTGAATCTGGTGCCTGCTCATAAGAACTTA AACAAGCAAGCGTATGATTTGGCAAAGGCGTTGCTGAAGAGGACCGCTCAAGCTATTGAACCCTATATTA CCAATTTTTTTAATCAGGTTCTGATGCTTGGGAAAACATCTATCAGTGATTTGTCTGAGCATGTCTTTGA TTTGATCTTGGAACTCTACAACATTGACAGCCATTTGCTGCTTTCTGTTCTGCCCCAGCTTGAGTTTAAA TTAAAGAGCAATGACAATGAAGAACGCCTCCAGGTTGTTAAACTCCTGGCAAAAATGTTTGGGGCAAAGG ACTCAGAATTGGCCTCTCAAAACAAACCACTCTGGCAGTGCTACTTGGGCAGGTTCAATGACATCCATGT GCCAATACGCCTGGAATGTGTGAAGTTTGCCAGCCACTGCCTCATGAACCATCCCGATTTAGCAAAGGAT TTAACAGAGTATCTTAAAGTGAGGTCACACGACCCTGAAGAAGCTATTAGACATGATGTCATTGTGTCTA TAGTAACAGCTGCTAAAAAGGACATTCTTCTTGTCAACGATCACCTACTCAACTTTGTGAGAGAGCGAAC GTTGGACAAACGGTGGAGAGTACGCAAAGAAGCCATGATGGGACTTGCCCAGATTTACAAGAAATACGCT TTACAGTCAGCGGCTGGCAAGGACGCTGCCAAGCAGATATGCTGGGTCAAGGACAAGCTGCTGCATATAT ACTATCAGAACAGCATTGATGACCGGTTGCTTGTTGAGCGGATCTTTGCTCAGTACATGGTTCCTCACAA CTTGGAAACTACGGAGCGGATGAAGTGCTTGTACTACTTGTATGCTACCCTGGACCTGAATGCCGTGAAG GCATTGAATGAAATGTGGAAATGTCAAAATCTACTCCGACATCAAGTAAAGGATTTGCTTGATTTAATTA AACAACCAAAAACAGATGCCAGTGTCAAGGCCATATTTTCTAAAGTGATGGTTATTACAAGAAATTTGCC AGATCCTGGTAAGGCCCAGGACTTCATGAAGAAATTCACACAAGTGTTGGAAGATGATGAGAAAATACGG AAACAGCTGGAGGCCCTTGTTAGCCCTACATGCTCCTGCAAGCAGGCTGAAGGCTGTGTGCGTGAAATAA CTAAGAAGCTGGGAAACCCTAAACAGCCTACGAACCCCTTCTTGGAGATGATCAAGTTCCTCCTGGAGAG GATAGCCCCTGTGCACATTGACACTGAGTCTATCAGCGCTCTTATCAAGCAGGTGAATAAGTCGATAGAT GGGACAGCAGATGATGAGGACGAGGGCGTCCCCACAGATCAGGCTATCCGCGCGGGCCTCGAGCTGCTGA AGGTGCTGTCTTTTACACACCCTATCTCATTCCACTCTGCCGAGACGTTTGAATCCCTGCTGGCTTGTCT GAAGATGGATGATGAAAAGGTGGCGGAAGCTGCCCTACAAATTTTCAAAAACACAGGAAGCAAAATCGAA GAGGATTTCCCTCACATCAGATCAGCCTTGCTTCCTGTCCTACATCACAAATCCAAAAAAGGACCACCTC GTCAGGCCAAGTACGCCATCCACTGCATTCATGCCATATTTTCCAGTAAAGAGACCCAGTTTGCACAGAT ATTTGAGCCTCTGCATAAAAGCCTGGACCCCAGCAACCTGGAGCACCTGATCACCCCGCTGGTCACCATT GGTCACATCGCTCTGCTCGCGCCTGATCAGTTCGCTGCCCCACTCAAGTCCTTGGTAGCAACTTTCATTG TGAAGGACCTTCTCATGAACGATCGGCTTCCAGGAAAAAAGACAACTAAGCTTTGGGTTCCTGATGAAGA AGTCTCCCCTGAGACAATGGTCAAAATTCAGGCTATTAAGATGATGGTTCGCTGGCTGCTCGGAATGAAA AATAATCACAGTAAATCAGGAACTTCCACCCTCAGATTACTAACTACGATACTGCATAGTGATGGGGACT TGACAGAACAAGGGAAAATTAGTAAACCGGACATGTCCCGCCTGAGACTTGCTGCTGGGAGTGCTATTGT GAAGCTGGCGCAAGAGCCCTGTTACCACGAGATCATCACACTGGAGCAGTACCAGCTGTGCGCACTGGCC ATCAATGACGAGTGCTATCAAGTCAGGCAGGTGTTTGCTCAGAAACTTCACAAAGGCCTCTCCCGCTTAC GGCTCCCACTAGAGTACATGGCCATCTGTGCTCTCTGTGCCAAAGACCCGGTGAAAGAGCGGAGAGCGCA TGCCAGGCAGTGCCTGGTGAAGAACATCACTGTGAGGAGGGAGTACCTGAAGCAGCACGCGGCTGTTAGT GAAAAATTATTGTCTCTTCTCCCAGAGTATGTTGTTCCGTATACAATTCACCTTTTGGCACATGACCCCG ACTATGTCAAAGTGCAGGATATTGAACAACTTAAAGATGTGAAAGAATGCCTTTGGTTTGTTCTGGAGAT ATTGATGGCTAAAAATGAAAACAACAGCCATGCATTTATCAGAAAGATGGTAGAAAATATTAAACAGACA AAAGATGCTCAAGGACCAGATGATACAAAAATGAATGAAAAATTGTACACCGTGTGTGATGTAGCCATGA ACATCATCATGTCAAAGAGCACCACATACAGTCTGGAGTCCCCAAAAGACCCAGTGTTGCCTGCTCGGTT TTTCACCCAGCCTGACAAGAATTTTAGTAACACCAAAAATTACCTGCCTCCAGAAATGAAATCATTTTTC ACTCCTGGAAAACCTAAAACAGCCAATGTCCTCGGAGCAGTTAATAAGCCGCTTTCATCGGCAGGCAAAC AGTCTCAGACCAAATCCTCACGGATGGAAACCGTGAGCAACGCGAGCAGCAGCTCAAACCCAAGCTCTCC TGGAAGAATAAAGGGGAGGCAATCTGAGTTGGAGAAGCCTAGAAGTCGGAAAAAAGCATCTGTCACAGAC CCAGAAGAGAAATTAGGTATGGATGACCTTAGTAAGCTGGTCCAGGAACAGAAACCTAAAGGCAGTCAGC GGGGCCGGAAAAGAGGCCATGCGGCTTCAGAATCAGAGGAGCAGCAGTGGCCTGAGGAGAAGAGGCACAA AGAGGAGCTCCTGGGAAATGAGGACGAGCAGAACAGCCCCCCGAAGAAGGGCAAGAGAGGCAGGCCACCA AAGCCTCTTGGGGGGACATCCAAGGAAGAGCCAGTGGTGAAAACATCCAAGAAGGGAAACAAGAAGAAGC CTGCACCTCCTGTAGTAGACGAAGATGAAGAAGAAGAAAGACAAATGGGAAACACAGAACAGAAGTCAAA GAGCAAACAGCAGCGCACGTCCAAGAGGGCTCAGCAGAGAGCAGAATCTCCTGAGACTAGTGCAGTTGAA TCCACACAGTCCACACCACAGAAAGGACGGGGAAGACCATCAAAAACGCCATCACCATCACAACCCAAAA AAAATATCCGTGTAGGACGCTCCAAACAGGTAGCTACTAAAGAAAACGATTCAAGTGAAGAAATGGATGT GCTTCAGGCCAGCTCTCCTGTCAGCGACGACACTACGCAGGAAGGAGCAGAAGAGGAGGATATTTCTGCA GGAAATGTACGGCGGCGAAGCTCCAAACGAGAGAGACGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001101805
Insert Size	4242 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001101805.1, NP_001095275.1
RefSeq Size	7071 bp
RefSeq ORF	4242 bp
Locus ID	304218

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