Irag1 (NM_001105210) Rat Untagged Clone
CAT#: RN200094
Mrvi1 (untagged ORF) - Rat MRV integration site 1 homolog (mouse) (Mrvi1), transcript variant 2, (10 ug)
CNY 10,930.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | Mrvi1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN200094 representing NM_001105210
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCACATTCCTGAGGATGAGGAGCCCCCCGGAGAGCCACAGGCAGCCCAGACCCAAGACTCTCCCT CTGCAGGACCGTTTTGCAGCCCTCCCACAATCATCCTGACGGGGGATGCCAGTTCACCTGGAGGAGAGAC TGACACAAATCTGGTCAAGAGGGCTCCCAGCCCCCACCGCAGGCTTTCCCACCGACACCTGAAGGTCTCC ACTGCCTCGCTGACTTCTGTGGACCCCTTGGGGCACGTCATTGACCTGGTAAATGACCAGCTGCCGGACA TCAGCATCTCAGAAGAGGACAAGAAGAAAAACCTGGCACTTCTAGAAGAAGCCAAGTTGGTTAGCGAGCG GTTCCTGACCCGCCGCGGGAGGAGATCAAGGAGCAGCCTCGGAGACGCCCCATCAGCTGTTTCTCCAAAC CTCAGCTCTGGAGCTTCTCCTGCATCCTCTAGGAGTTGCTCGTTCACCATCTCCACACCACCAGGTTTTG ACACATGCAGTGGCCCACAGTCCCCTCTGCCGGGATCACCAGCACAGCAAAAGGGACATGAGGATGATGT CTTCTCGTCTCACCTCGGAGAGCCTAATGCCTCCAAAGGGCTAGCTGACCGGAAGCAGAATGACCAGAGG AAAGTGTCTCAAGGCAGACTGGCTCCTCGCTCTCCCACAGTGGAGAAATCCAAAGAACTTTCAGTAGAAC AAAAGGAAAACTTCGATCCCCTTCAGCATCTGGAGGCCACACCCATGGCTCAGACCTCTGGAGCAAGCGT CAGTGGGAAAATGGCGCTGAACAGTCCCCAGCCTGGCCCTGCGGAGATGGAGCTGGGGAGGCAGCTTCTG AAGACAGCCAGGGAAGACAACCCTCTGCCCAGAACCACAGTCCAGGGCTCAGGAGGAACGGCCTCCCCAC ATTCTCAGGGACAGGGCTCTGCTGGAGAGCTTATGGGGCCCAAGGCTGGCTCCAAGGCTGAGCCGAGGCC TCCTGTGTCCCGGCCCCCTCTGATTCGGGGGGTCTCCTGGGACAGCAGCCCTGAAGAACCTGGTCCCCTG CTGCAAAAGGTGCTCGCTAAGCTGCCTCTGGCAGAGGAAGAAAAGCGGTTTCCAGGCAAAGCCAAGCCAG CCAAGCCACCGGGGCTCAAAGACTTCCAGATACAAGTACAGCCGGTGCGCATGCAGAAGCTGACCAAGCT TCGGGAGGAACACATCCTGATGAGAAACCAGAACCTGGTGGGGTTCAAGCTCCCTGAACTGAGTGAAGCT GCAGAGCAAGACAAAGGAGTCTCATCTGAGCTTGCCCCAGCAGCTGAGGAAGAAGAATCAAAGAGTGGTC TGGATGTGATGCCCAACATTTCTGACATATTGCTACGTAAACTTAGGGTTCACAAGTCACTCACTGGAAG CGCCCCTCCGCTCACAGAAAAGGAAGTCGAGAACGTGTTTGTACAACTGTCCTTGGCCTTTAGAAATGAC AGCTACACCTTGGAGTCTAGGATTAACCAGGCTGAAAGGGAACGCAACTTGACGGAAGAGAACACCGAGA AGGAACTGGAAAACTTCAAAGCCTCTATTACGTCCTCAGCATCCATCTGGTACCACTGCGAGCACCGGGA GACCTACCAGAAGCTTCTGGAAGACATCGCAGTCTTGCATCGTCTGGCTGCACGCCTCTCCAGCCGGGCT GAAGTGGTGGGTGCTGTGCGCCAGGAAAAGCGCATGTCAAAGGCCACGGAAGTGATGATGCAGTATGTGG AGAATCTGAAGAGAACGTACGAGAAGGACCATGCTGAGCTCATGGAATTTAAGAAACTCGCAAATCAGAA TTCTAGTCGCAGCTGTGGGCCCTCTGAAGACGGGGTTCCTCGCACGGCGCGATCTATGTCCCTCACGATG GGAAAGAACATGCCCCGTCGGAGGGTCAGCGTTGCTGTGGTTCCCAAGTTTAATGCCCTGAACCTGCCTG GCCAAGCCCCCAGCTCATCACCCATGCCCTCTCTCCCAGCTCTGTCTGAATCGACCAACGGGAGAAGCAG CATCTCCACCTCTCCAGTGCTGCCTGCACTTTTGGAAAATGGAAAGACAAATGCAGAGGCTAACTGTGAA GTGTGCGCCCCTGTGCCACTCCCAAGCTACCCGGAGGAGACGAGCCAAGAGGCCAAGGCCAGAGCAGAGG AAGAAGCCTACAACAAAGGATACCAGGAAGGGGTAAAGAAGACCGAAGAACTTCAAGACCTGAAGGAGGA GGAAGAGGAACAGAAGGCAGAGAGTCCTCAGGAACCAGAAGAGGTCGAAGAAACCCAGGAAGACGAGAAG GACCAGAGAAGCAGCAAACTTGAAGAACTGGTCCACTTCCTACAAGTCATGTATCCCAAATTGTGTCAAC ACTGGCAAGTGATCTGGATGATGGCCGCTGTGATGCTGGTCTTGAGTGTTGTGCTTGGGCTCTACAGTTC CTATAACTCCTGCACGGAGCAGGCCGACGGGCCCCCTGGGAGATCCACCTGCTCTGCAGCCCAGCGGGAC TCCTGGTGGAGCTCGGGACTCCAGCAAGAGCTGCCAGCAGAGCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001105210 |
Insert Size | 2568 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001105210.1, NP_001098680.1 |
RefSeq Size | 6182 bp |
RefSeq ORF | 2568 bp |
Locus ID | 308899 |
Gene Summary | This gene is similar to a putative mouse tumor suppressor gene that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein, which is found in the membrane of the endoplasmic reticulum, is similar to Jaw1, a lymphoid-restricted protein whose expression is down-regulated during lymphoid differentiation. Studies in mouse suggest that MRV integration at the homologous mouse gene induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation, and thus this gene may function as a myeloid leukemia tumor suppressor gene. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013] Transcript Variant: This variant (2) contains an additional 5' exon, is differently spliced at the initial coding exon, and initiates translation from an in-frame, downstream AUG compared to variant 1. The resulting isoform (b) has a shorter N-terminus compared to isoform a. Sequence Note: This RefSeq transcript and protein were derived from genomic sequence because no rat transcript was available. The extent of this transcript and the encoded protein are supported by orthologous mouse data. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR200094 | Mrvi1 (Myc-DDK-tagged ORF) - Rat MRV integration site 1 homolog (mouse) (Mrvi1), transcript variant 2, (10 ug) |
CNY 10,930.00 |
|
RR200094L3 | Lenti ORF clone of Mrvi1 (Myc-DDK-tagged ORF) - Rat MRV integration site 1 homolog (mouse) (Mrvi1), transcript variant 2, (10 ug) |
CNY 12,730.00 |
|
RR200094L4 | Lenti ORF clone of Mrvi1 (mGFP-tagged ORF) - Rat MRV integration site 1 homolog (mouse) (Mrvi1), transcript variant 2, (10 ug) |
CNY 13,970.00 |