Irag1 (NM_001105210) Rat Untagged Clone

CAT#: RN200094

Mrvi1 (untagged ORF) - Rat MRV integration site 1 homolog (mouse) (Mrvi1), transcript variant 2, (10 ug)



  "NM_001105210" in other vectors (3)

CNY 10,930.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Mrvi1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN200094 representing NM_001105210
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCACATTCCTGAGGATGAGGAGCCCCCCGGAGAGCCACAGGCAGCCCAGACCCAAGACTCTCCCT
CTGCAGGACCGTTTTGCAGCCCTCCCACAATCATCCTGACGGGGGATGCCAGTTCACCTGGAGGAGAGAC
TGACACAAATCTGGTCAAGAGGGCTCCCAGCCCCCACCGCAGGCTTTCCCACCGACACCTGAAGGTCTCC
ACTGCCTCGCTGACTTCTGTGGACCCCTTGGGGCACGTCATTGACCTGGTAAATGACCAGCTGCCGGACA
TCAGCATCTCAGAAGAGGACAAGAAGAAAAACCTGGCACTTCTAGAAGAAGCCAAGTTGGTTAGCGAGCG
GTTCCTGACCCGCCGCGGGAGGAGATCAAGGAGCAGCCTCGGAGACGCCCCATCAGCTGTTTCTCCAAAC
CTCAGCTCTGGAGCTTCTCCTGCATCCTCTAGGAGTTGCTCGTTCACCATCTCCACACCACCAGGTTTTG
ACACATGCAGTGGCCCACAGTCCCCTCTGCCGGGATCACCAGCACAGCAAAAGGGACATGAGGATGATGT
CTTCTCGTCTCACCTCGGAGAGCCTAATGCCTCCAAAGGGCTAGCTGACCGGAAGCAGAATGACCAGAGG
AAAGTGTCTCAAGGCAGACTGGCTCCTCGCTCTCCCACAGTGGAGAAATCCAAAGAACTTTCAGTAGAAC
AAAAGGAAAACTTCGATCCCCTTCAGCATCTGGAGGCCACACCCATGGCTCAGACCTCTGGAGCAAGCGT
CAGTGGGAAAATGGCGCTGAACAGTCCCCAGCCTGGCCCTGCGGAGATGGAGCTGGGGAGGCAGCTTCTG
AAGACAGCCAGGGAAGACAACCCTCTGCCCAGAACCACAGTCCAGGGCTCAGGAGGAACGGCCTCCCCAC
ATTCTCAGGGACAGGGCTCTGCTGGAGAGCTTATGGGGCCCAAGGCTGGCTCCAAGGCTGAGCCGAGGCC
TCCTGTGTCCCGGCCCCCTCTGATTCGGGGGGTCTCCTGGGACAGCAGCCCTGAAGAACCTGGTCCCCTG
CTGCAAAAGGTGCTCGCTAAGCTGCCTCTGGCAGAGGAAGAAAAGCGGTTTCCAGGCAAAGCCAAGCCAG
CCAAGCCACCGGGGCTCAAAGACTTCCAGATACAAGTACAGCCGGTGCGCATGCAGAAGCTGACCAAGCT
TCGGGAGGAACACATCCTGATGAGAAACCAGAACCTGGTGGGGTTCAAGCTCCCTGAACTGAGTGAAGCT
GCAGAGCAAGACAAAGGAGTCTCATCTGAGCTTGCCCCAGCAGCTGAGGAAGAAGAATCAAAGAGTGGTC
TGGATGTGATGCCCAACATTTCTGACATATTGCTACGTAAACTTAGGGTTCACAAGTCACTCACTGGAAG
CGCCCCTCCGCTCACAGAAAAGGAAGTCGAGAACGTGTTTGTACAACTGTCCTTGGCCTTTAGAAATGAC
AGCTACACCTTGGAGTCTAGGATTAACCAGGCTGAAAGGGAACGCAACTTGACGGAAGAGAACACCGAGA
AGGAACTGGAAAACTTCAAAGCCTCTATTACGTCCTCAGCATCCATCTGGTACCACTGCGAGCACCGGGA
GACCTACCAGAAGCTTCTGGAAGACATCGCAGTCTTGCATCGTCTGGCTGCACGCCTCTCCAGCCGGGCT
GAAGTGGTGGGTGCTGTGCGCCAGGAAAAGCGCATGTCAAAGGCCACGGAAGTGATGATGCAGTATGTGG
AGAATCTGAAGAGAACGTACGAGAAGGACCATGCTGAGCTCATGGAATTTAAGAAACTCGCAAATCAGAA
TTCTAGTCGCAGCTGTGGGCCCTCTGAAGACGGGGTTCCTCGCACGGCGCGATCTATGTCCCTCACGATG
GGAAAGAACATGCCCCGTCGGAGGGTCAGCGTTGCTGTGGTTCCCAAGTTTAATGCCCTGAACCTGCCTG
GCCAAGCCCCCAGCTCATCACCCATGCCCTCTCTCCCAGCTCTGTCTGAATCGACCAACGGGAGAAGCAG
CATCTCCACCTCTCCAGTGCTGCCTGCACTTTTGGAAAATGGAAAGACAAATGCAGAGGCTAACTGTGAA
GTGTGCGCCCCTGTGCCACTCCCAAGCTACCCGGAGGAGACGAGCCAAGAGGCCAAGGCCAGAGCAGAGG
AAGAAGCCTACAACAAAGGATACCAGGAAGGGGTAAAGAAGACCGAAGAACTTCAAGACCTGAAGGAGGA
GGAAGAGGAACAGAAGGCAGAGAGTCCTCAGGAACCAGAAGAGGTCGAAGAAACCCAGGAAGACGAGAAG
GACCAGAGAAGCAGCAAACTTGAAGAACTGGTCCACTTCCTACAAGTCATGTATCCCAAATTGTGTCAAC
ACTGGCAAGTGATCTGGATGATGGCCGCTGTGATGCTGGTCTTGAGTGTTGTGCTTGGGCTCTACAGTTC
CTATAACTCCTGCACGGAGCAGGCCGACGGGCCCCCTGGGAGATCCACCTGCTCTGCAGCCCAGCGGGAC
TCCTGGTGGAGCTCGGGACTCCAGCAAGAGCTGCCAGCAGAGCAGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001105210
Insert Size 2568 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001105210.1, NP_001098680.1
RefSeq Size 6182 bp
RefSeq ORF 2568 bp
Locus ID 308899
Gene Summary This gene is similar to a putative mouse tumor suppressor gene that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein, which is found in the membrane of the endoplasmic reticulum, is similar to Jaw1, a lymphoid-restricted protein whose expression is down-regulated during lymphoid differentiation. Studies in mouse suggest that MRV integration at the homologous mouse gene induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation, and thus this gene may function as a myeloid leukemia tumor suppressor gene. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013]
Transcript Variant: This variant (2) contains an additional 5' exon, is differently spliced at the initial coding exon, and initiates translation from an in-frame, downstream AUG compared to variant 1. The resulting isoform (b) has a shorter N-terminus compared to isoform a. Sequence Note: This RefSeq transcript and protein were derived from genomic sequence because no rat transcript was available. The extent of this transcript and the encoded protein are supported by orthologous mouse data.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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