Tacc2 (NM_001004415) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200092 representing NM_001004415 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACGAGAACAGCACCTCGGACCACCAGAGGACTTCTTCAGTTCAGAGTCTAAGATCACTGCAGC CACCCGGGAACAGTCAAACTCCACAGAAGCAAGGAGACTCACCTGGATCCGGCGCTGCAAGCACTGGGCT TGGAGCCTCCTGCTCTGCTTCTGAAAGTGTTGCCAGCCTGGATCCGAGCACTGTGTCCCCAGAGGTGACT AAGCCAGGGGAGCACCCACAGGAAGCCAGGGACCCAGAAGATTCTCCACTGCCCAGATCATCTCTGCCCT GGGAACAGCCGTACCCCTCAGCCTCCATGCCTTTCACGGAGGGCCTCTCAGAAGGTTGCTTGGCAAACTC AGAAGCGGAAGTACCTGAAGATGGTCCCCTCACAAATCACCTTAGAGTGGAACCCTCCCCCAACGGGCCA GGGGATATCTTACTGACATTTACTCCTGAAGTGGATGGCCCCGCACAGCGCACAGTGGCTGCCAGAACCC CCAGTGCTGAAGGAGACCAAGAGCTGAAGGAAGAGGGACAGAGCTCATCCTCCAGTTGCACTGGCCAATC GCCAGGAATTTCCTTCGTTCCTCTCCAGGAGCCAAAGACAGAGCTGCTCTATGGAAAGGGTGACTGGCCT CGTGACTTTGAGTTCCAAAGGAAAGATGATATCGACAATTGTTCCTCCCCAGAGTCAGTCACTAAAGCCC CTGCTGCCACTCAGCCGGGGATGCAAAATTCAGCTGTCCTTGAGTGCCCCCCAAAACTTGAGACCATGGT CCCACAAGATTCAGTCCTAAGATCCTCAGACAGTAAAAAAGGCAGATCGGAAGTGCTACCTATGGATTTG GCCAAACACGTGGAATTTCTCGGAGGCTGCCATCCCTCTAAAGGCAACTCTGGAGCTAACCCTGGAGCAG GAACAAGCCCTGCCTCCCAGGAAAGCTGTCAGCTGAGAGTGGGAATGCCTCTGCCATCTGCAGAACTACC TTCAGGCCCACAAGGCCTTACCACAGCACGAGCAGACGGTGGCTCCTGGGAAGAGACTCTGGACACCAGC AGCGCTCAGAGCCAACCACAGACAGGGACATCAGGAGCTGAGCCCCAGCAAGTTGTCTGTGTGCCAGCAG CCAGCCAGCCGGGTGGGAGCTTGCTTCTGAGCGCCAAGGCCCCTGGATTCACTGGAACAGAGGAAAGACA TACGGCTTCAAGTCCCACTGCGCGCCTGACAGCTTGGAGTTCTACTGCCTCAGAAGAACCCACGGAGGCT GTTTCTGAGACGAAGATGTCAGTTTCTGCAGATCTGGCTGCCACAGGACAGATGGGTCCAGGGGTGGTGG AACTGAAGCCAACATCAGATCTTAGAAGAACACAAGAACAACCTCCTGAAGGTAGCTGCCTCCAGTGGGT TCCTGCGCCTTTCTTGCAGGGGCGAAATGATACAATCCAAGAAGGGCTGTTGCTGCCACCTTTCTCCCAT CAGGTTTCAAATGAAAGTTCTAGAAGGTCAATGGGACCAACTGATGATCCCAAGGCCTCCAAGAATACTG AAAATAGAGTTGTGGCTAGAGAGGAAAGCATACCGCATGGAGACAACCGTAAGGGACAGCAAGAAGCCAC CAGAGCCCTTGCTGGTACAGAGCTTAGGAACCATGACAAAGAGAAGTCTCATGCCTTTCGTGCCAAGCCC CGTGGGGACACTGAGAAAGATGACATTTCAGAGCTACACAGTAATAAAGAGAACAAGATTGTCCCCAGCT CGGACTCAACACAGCCACCTAGGCAGGAGGTTGCTGAATCCCATATGACCGACACAGCTACCTCACTGCA CGAAGTCCACGAGGAGGACCCCATCCTGTCCTCCGCATCGGATGGAACTGGTGAGCACTTTCTTCCCCAA GATGCCATCTGGGAAAGTTCAAGATTGCAGACTGAAAGCTCTGTCATGCTTCAGGCCAGGGGAGGATTGG AAGCAACAGAGTCACTTCCTGTTTTAGAATCTGAGAAAGCGGATGCCCTGCCTGCTACAGCTATGGAGGA GGAGGCACCCAGAGCTGCGGAGATGGAGGATGTACTGGAAGTAAAGAAGGACCACTCACCCTTGCATCAG CCCTACAGCTGTGCCGGGCAAGGCTTGCCGATGTCCCCAGGCCAACCTTGTGGGCTGGAGAAGGTTGAAC CTGGACAGGAAGATCATGCTGATACGTCATCTCCCCCCAATGGGAAAAATTCAACAATAGGCGACGGACT TACCGTGCCCCGTCTGGAACAAGACGGTCAGAGAAAACTGTCCTGCCCAGAGGCCAGTCTCCCACCACCT TTACAGAACCCTCTCCTGTCATCTCAACTAGACCCAGATGCAGCCATCTCTGCTGTTCTCTGTGACAAGG ACCAGTCACCTACAAATGGGCAAAGGGCTTGTCTGTGTGGCCCAGGTCTGAAGGAGCAGAGTACAGATCC TTCTCCAATCCCAGTGTCTGAGGAAGGGAAGCCTTGGGCAGACGTGAGCTTGTCTACTCAAGGAGGAAAG GAACAGGGGTCAGAACTTCCCTCCAAAGGCAGCCTGTGCGATACTCCAAGTTCATCACCCAAGGGTACAG TTCTGGGAGGGGCACCGTCAGAGATGTCAGAACTGTCAACCCCACTGGGACAAGAGCTGCCTGCACTAGG GGGAAATGAGCAAGAGAACACGGGCAGAAACAGTCAACCCTCGGAGATCCAGCCTCCTTCAGCCTCTCTT ACAGGACACTCGGGTGGAAAGGATAGTTTCTGTGTTGGGCAGGGGCTGAGCAAGTCCCAACAGGAGCTGG CTGATGCTTTGCAAACAGGAAGCCAGCGTGAAGAAGCACGTTGTGGGGACTCGGGCATTTCTGAAGCCAC TGGCGTCCTGCCTCCGCCTCGGGGCTTGGAGAAGATAGAGACAGCAAGTAGAAACATAGCGGAGGCCCCA CCTGGTCCACCTGACTCAGTAGCTCTCTTGGATACAGCATACTGCTCTCCAGACCCAGTGCCTACCAATC CCGGAGTCGCACCCGCCCACGATGCCCTAGTGAGCGAGGCCTGTGATGAGAGCCAGAAAGAGTCGGTCCC ACAGCTGGATATGGAGCAGCGGGCCACCTTAGGTCCAGAAGAACAGAGTCCTCTTGGAAGTTTCCGGAGA GCAGAGGAACAAGATAGTAAAGAAGGATCAGCAGAGGTGAGTGTCGATGCCAGCGACGGAGACCCCAAGA TGCAGCAGGCGTCAGAGGCACCAGAAGCTGCTCTGAGTAGTGGCTTCTTCCAGGCTGACCAGAGCCTCCC CTCCACCCTGAAGGAGCCACGCCAGCCTGCGAAGCTTGAGCCCAGCTGTGGGGATGCTTTGCTGCCAGCT GGAGAATCGAATGGGATTCCCAGAAGTACTGTGGGTGTTCTGACACACCGCGCTGTTTCAGGCCCACAGA GCCTTCTGCCGGCTGAACCACCTGAAGAGACTGTGCCTGACACCCCTTACCTGCATATCAGTGGTGCTGC CAAGAAGGATGCCGAAGATGCTAGCATGAGAGCTGTTTTCTCTGAGGACCCCGGAGCACCTCATGAAAGT CCTATAAAGGAGCCTCCACCTGTGGAAAACGACACCCCTGGAAAGAGCCCTGCTGTCTCCTTCACCACAG ACTTGCAACCAGGAACTGCACATGGGGAAATCTCCGTAGCAGGGGATGGCGATGGTATCCCCCAAGCTGG AACCACTGAGGGACATGTGAGTCTCACGCCCTACCTGGACAGGATGCCTCTCCTGGTGAGAGATGAGCAG GTGGCAAGAGAGATGCATGTGGCTGCCACCCCAGAAACAAATGCTAGGCCCTCTGAGACTGCAGTGTGCC CTGCCAGCGAGGAAGCAGCAGGAGAGACAGGGGGCAGCAGAGAAAGGGCAGTAGAACTTACCCCAGACCT GATGGTTGTTACATCAGGTAGTGAGGGTGCAAGCTCCAAGCAGCCCAGCGTAATCAAGGGGCTTCCTGAC TTCAGGGAGCACATCACCAAGATCTTTGAGCAGTCTGTGTTTGGAGCCCTGGCTGCTGATCGGACCCATA GTGTGCCTAGTAAAAAGTCAGGAGTTCCAAGCAGTATGCTGGTTGAGGGCCCCGTGATCTCACTGGACTC AGGGAAGCTTCCAGATGGGGTGCAAGGAATGGCTGCTGCCCCTCTCCCTGTACCTCCTGTAGGACTCCAG GTGGAGAAGAAGCAAGAGTCAGGTGTCAAGGCTGAGGTTTCTCATCCAGTTCCCCAGGATCCAGCACCAG AGAAGCTGATGGGTCTGGCGGAGGCAGCCTTGGAGGAGAGCCTGCCAGGTGCCATTGTTGAAGGAGAGGT GACCCGTGTCCCACTGACAGTGACAAGTGAGAGGCTAGAGGGGACTGGGGAGCCTGGCCAGGGAGCTCAG GCTCACACACAGCAGGCAAGAAGCAGGCAGGAATTAGTTGTGGGTCTTCCCTCTTCTAAAGCTGTTCAGG GGGTACCTGCAGAACCAGCTCCTGGCTTCCCTGTGGATCCCCAGAGTCATGCAGATGCAGATGAGGCTTC TTCTCAAGGTGACAAAAGCCATTCTGTAAAAGAGTACCTGGAAACGCTGCCCAGCAACAGTCCACACGGA ATAAATGGTGCCCAGGACTCTGTCCACACCAAGGGTCCAAGTAATCTATTAGGATCCACCTGTGCCCTGG ATGGCTCCTCTCCTCATGGAAGTGTTTTGAATATGCCCGAATCCAACAGTGAGCCCTGGATCCCTTCCAC ACTTGGGGGTGAGAGACAGCTGGAAGCTGCTGTCTGTACCTCAGATAAGCAAAACGTGCTGGAAAACCAG GATGGCCCCAAAATGTTGGCTAGAGAAGTGTTGGCGATTCCCCTGGGGCCTAGCAACATGGCAGGAGCTG CTGAGGAAGCAGAAGGTGATGTCACCCCAAGCAGGCCTGAGACGTGGGCAGAGGTGTCTGGTGACCTCCT TGAAACAGGTACTACAAGGATGCTTCCCCATGAGGCAGGTGACTCAGCACCGCCTGGGAGCTATCGGGAT TCAGGATGCTCCAACAGGACTCAGGTGATGGAGGACACAGCCACCCTTCCAGGGGACAGCCAGGTTGGAT GCCAGCAGGCCAAGGAACCGCTGGGCCCTCAACTCCCAGCTCCTTACGGGAAGACGAGTGTCTCTTCCCC TCCAGAGCCTGATGAAAGCAGTGACGAGAAGCTGCACCTCCTGGCTCCAGAAGTCCTCACTGACAGCATC CCTGGAACCAATGATGTCATCCAGTCAGCTGCTCCCGAAGACCTGGGACACCCACCCTTAGCTGATTCTT CCCGCTATGGTGTTACTGTCAGCTCAGTCTCTACACATCTGACAGTCCAGAGCACCTCCCCATCTGCTGC CCATGCAAGTCTTGCTCCTGTGGCCTCAGAACATGCAGCCTTGGCTTCCGCCGCAGCTGGTCCTGGAGTA GAAACCCCCACTGCCTCCTGCCAACACCTGGCCAAGAACATAAACAGGAGTTCCGACTCTGAGGAAGCCT TTGAGACCCCCGAGTCAACAACCCCTGTCAAAGCTCCACCAGCTCCTCCCCCTCCACCCCCTGAAGTCAC CCCGGAGCCTGAGGTCATCGAACCACCAGCCCCAGAAGAACCAGGATGCATTTCTGAGCCGGTCGTGGTT CCCGATGGCCCTCGCAGCGAGTCCGTGGAAGGAAGCCCTTTCCGTCCTTCGCACTCCTCCTCGGCGGTGT TCGATGAAGACAAGCCGATAGCCAGCAGCGGGACATACAACTTAGACTTTGACAGCATCGAGCTGGTGGA TAACTTTCAGAGTTTGGAGCCATGCTCTGCCGACTATAAGGGTCAGGAGTGTAAGGTGAGCACGCGGAGG AAATCCACCGAGTCCGTGCCACCCTCCAAGACCACGCTGTCCCGCTCGCTCAGCCTGCAAGCCAGCGACT TCGACGGTGCTTCGTGCCCCGGCAGCCCGGAAGCTGGGACCCTTGCCACAGATGCGTGTGGCACAGGATC CAACAGTGCTTCTAGCACCCTTAAGCGAACTAAAAAAACACGGCCACCTTCCTTAAAAAAGAAACAAGCT ACCAAGAAACCCACAGAAACCCCCCCAGTGAAGGAGACCCAACAGGAGCCAGGTGAAGAGAGTCCGGTGC CCAGTGAGGAACACTTAGCACCAGAGACAAAGACAGAATCGGCCACACCTGAGGGCACTGGTTGCACCTT GTCAGAAGAGACATCTCTGGAGTCTGCTGCTGTGCCCACAGCTACCTGTCCTCTGACTTTGGAGAGTGTG GAAGACGTTAGCCCTCTGGTTTCTGGAGGTGGCAGAGTGCAGAACTCACCCCCTGTGGGGAGGAAATCAG TGCCTCTTACCACAGCCTCTGAGGCAGTGGAGGTGACGCTGCCGGACGGTGGGGGGCAAGAGGACTTGCC TGCCAAAGGGCTGTCAGTGAGGCTGGAGTTTGACTACTCTGAGGACAAGGGAAGTTGGGAGAGTCAGCAG GAGAACGCCCCTCCCACCAAAAAGATAGGCAAGAAGCCAGTTGCCAAAATGCCCCTAAGGAGGCCAAAGC TGAAAAAGACCCCAGAGAAACTGGACAACACTCCTGCCTCACCTCCAAGGTCCCCTGCTGAACCCAGTGA CATCCCTATTGCTAAAGGTACCTACACCTTTGATATAGACAAGTGGGATGACCCCAATTTTAACCCTTTT TCCTCCACCTCGAAAATGCAAGAGTCTCCCACACTGTCCCAACAATCGTACAACTTTGACCCCGACGCCT GTGAAGAGTCCCTTGACCCCTTTAAGGCATCCTCTAAGACCCCCAGTTCACCTTCTAAATCCCCAGCCTC TTTCGAGATCCCAGCCAGCACCATCGAAGCGGACGGGGATGGGTTGAATAAACCCGCCAAGAAGAAGAAG ACTCCATTGAAGACTGACACATTTAGGGTGAAGAAGTCTCCAAAGCGGTCTCCTCTGTCTGATCCGCCTT CTCAGGACCCCACTCCAGCTGCCACACCGGAAGCACCTCCAGTCTCTACCGTGGTCCACGCCACAGATGA GGAAAAGTTGGCCGTCACTAGCCAGAAGTGGACATGTATGACAGTGGACTTGGATGCTGACAAACAGGAT TTCCCGCAGCCCTCAGACCTATCTAACTTTGTAAATGAGACCAAATTCAATTCACCCTCGGAGGAGCTGG ACTACAGAAACTCCTATGAAATTGAATACATGGAAAAGCTTGGCTCCTCCTTACCTCAGGACGATGACAC TCCGAAGAAGCAGGCCTTGTACCTTATGTTTGACACCCCTCAGGAGAGCCCCGTCAAGTCTCCTCCGGTC CGGATGTCGGATTCCCCAACTCCATGTTCAGGGTCGAGTTTTGAGGACACCGAAGCCCTGGTGAATGCAA CAGCAAAGCTCCAGCATCCAGTCCCTAGAGGGCTGGCCTCCAATCAGGAGCCTCTCTTGCAGCTGCCGGA AAAATCCTCCCAGAAGGAGCTGGAGGCCATGGCCTTGGGCACCCCCGCAGAAGCAATTGAAATCACAGCT CCAGAGGGTGCCTTTGCCTCTGCAGATGCCCTCCTCAGCAGGCTGGCCCATCCTGCTTCTCTCTGTGGTG CACTTGGCTATCTGGAGCCTGATTTAGCAGAAAAGAACCCCCCAGTATTTGCCCAGAAACTTCAGGAGGA ATTAGAGTTTGCTGTCATGCGGATAGAAGCCTTGAAGCTGGCCAGGCAGATCGCCCTGGCTTCTCGCAGC CGCCAGGACACCAAGAGGGAAGCCACTCACCCACCAGATGTCTCCATCTCCAAAACTACCTTGTACTCCC GCATCGGGCCCACCGAGGTGGAGAAACCCCCAGGCCTTCTGTTCCAGCAGCCAGACCTGGACTCTGCACT CCAAGTTGCCAGAGCAGAGGTCATCGCCAAGGAGAGAGAAGTCTCAGAGTGGAGGGATAAATATGAAGAA AGCCGGCGGGAAGTGGTAGAAATGAGGAAAATCGTGGCTGAATACGAGAAGACCATCGCACAGATGATCG AGGATGAACAGAGAGAGAAGTCCATCTCCCACCAAACCGTGCAGCAGCTGGTGCTGGAGAAGGAGCAAGC CCTGGCTGACCTGAACTCTGTGGAGAAGTCTCTGGCTGACCTCTTCAGGCGATACGAGAAGATGAAGGAG GTGCTGGAAGGCTTTCGAAAGAATGAGGAGGTGCTGAAGAAGTGTGCACAGGAGTACCTGTCCCGCGTGA AGAAAGAGGAGCAGAGGTACCAGGCCCTGAAGGTGCATGCAGAAGAGAAACTGGACAGAGCCAATGCAGA GATTGCCCAGGTTCGAGGCAAGGCCCAGCAGGAGCAGGCGGCCTACCAGGCTAGCCTGAGGAAGGAGCAG CTTCGAGTGGATGCTCTAGAAAGAACGCTGGAGCAGAAGAATAAAGAGATAGAAGAACTCACCAAGATTT GTGACGAACTGATTGCCAAAATGGGGAAAAGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001004415
Insert Size	8505 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001004415.2, NP_001004415.1
RefSeq Size	9192 bp
RefSeq ORF	8505 bp
Locus ID	309025
Gene Summary	This gene encodes a member of the transforming, acidic coiled-coil (TACC) family of proteins. Members of this family are centrosomal proteins that interact with microtubules and tubulin. TACC proteins are thought to be involved in centrosome/mitotic spindle dynamics and gene regulation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1), also called the long form, represents the longer transcript. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were predicted based on rat transcript alignments and on homologous protein alignment with mouse NP_001004468.1.

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