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Cdc14a (NM_001134856) Rat Untagged Clone
CAT＃: RN200086
Cdc14a (untagged ORF) - Rat CDC14 cell division cycle 14 homolog A (S. cerevisiae) (Cdc14a), transcript variant 1, (10 ug)
CNY 7,700.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200086 representing NM_001134856
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGGAGTCAGGGGAACTAATCGGGGCTTGCGAGTTCATGAAAGATCGATTATATTTTGCTACTT TAAGGAATAGACCAAAAAGCACAATAAATATCCACTATTTCTCCATCGACGAGGAGCTGGTCTATGAAAA TTTCTATGCAGATTTTGGACCTCTGAACTTGGCAATGGTGTACAGATACTGCTGTAAGCTAAACAAGAAA CTAAAATCATACAGTTTATCAAGAAAGAAAATAGTGCACTACACCTCTTTCGACCAGCGGAAAAGAGCAA ACGCAGCATTCTTGATAGGTGCTTATGCCGTCATCTACTTAAAGAAGACACCAGAAGAAGCGTACAGAGC TCTCCTGTCTGGCTCAAACCCTCCTTATCTTCCATTCAGGGATGCGTCCTTTGGAAATTGCACTTACAAC CTCACCGTCCTTGACTGTTTACAAGGAATCAGAAAGGGATTACAGCATGGATTTTTTGACTTTGAGACAT TTGATGCGGATGAATATGAACACTATGAGCGAGTTGAAAATGGGGACTTCAACTGGATCGTTCCAGGAAA ATTTTTAGCATTTAGTGGACCACATCCTAAAAGCAAAATTGAAAATGGTTACCCTCTCCACGCTCCTGAA GCCTACTTTCCGTATTTCAAAAAGAACAACGTGACAACAATCGTGAGATTGAATAAAAAGATTTATGAGG CGAAGCGCTTCACAGACGCTGGCTTCGAGCACTACGACCTGTTCTTCATAGATGGCAGCACCCCCAGCGA CAACATCGTGCGAAGATTCCTGAACATCTGTGAGAACACGGAGGGGGCCATTGCGGTCCACTGCAAAGCT GGTCTGGGGAGAACTGGGACTTTGATAGCCTGTTACGTCATGAAACACTACAGGTTCACACACGCTGAAA TCATCGCTTGGATCAGAATTTGTCGACCAGGCTCCATCATTGGACCCCAGCAGCATTTCCTGGAAGAGAA ACAAGCATCATTGTGGGTCCAAGGAGACATTTTTCGATCCAAACTGAAAAATAGACCGTCCAGTGAAGGA AGTATTACTAAAATTATTTCTAGCTTGGATGATATGTCTATTGCTGGGAACTTATCTAAATTACAAAGCA CAGAAAGGATTGGAGAGAATAATTTTGAAGACGAAGATATGGAAATTAAAAACAACGTAACGCAGGGAGA CAAACTACGTGCCTTAAAAAGCCAGAGGCAGCCCCGCTCCTCGCCATCCTGTGCATTTAGGTCAGATGAT ATGAAAGGACACCAAAGGGCAGTGGCCCAGACTTTCAGATTAAGTTCTTCACCACAACCAACAGTGCCGA CTGTGAAGACCCCCAAAGTGTGTTTGTCCCCTTCAGTGACAGCCAAAAGGATAAGCAGAGGTTCTCTGTC TTCAGGAGCAAACATAAGAAGCTTCTCCATAAATTCCCGGCTAGCCAGTTCCCTGGGGAACTTGAATTCT GGGACAGAAGAAACTGAGAACAAAAAGACCACATCACCCATCAAGGCTGCTTTCATAGCCAGCCCGTTCA CCAGCTTCTTGAATGGCAGCACCCAGACACCTGGCAGAAACTACCCTGAGCTCAACAACAATCAGTACAC CAGAAGCAGCAACAGCAGCAGCGGCGGCAGTCTTGGGGGCAACCTGAACAGCTCTCCAAGCGCCAAGCCC GAGGAGCATAACACCATCCTTAGACCTTCCTTCTCGGGGGCGCTCTCTTCCTCCTCAGTGAGATTCCTGA GCCGTTCTATACCTTCCCTTCAGTCTGAATATGTTCATTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001134856 |
| Insert Size | 1794 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001134856.1, NP_001128328.1 |
| RefSeq Size | 4476 bp |
| RefSeq ORF | 1794 bp |
| Locus ID | 310806 |
| Gene Summary | The protein encoded by this gene is a dual-specificity phosphatase that preferentially dephosphorylates cyclin dependent kinase substrates to regulate the cell cycle. In human cell lines, this protein localizes to interphase chromosomes, and depletion of the transcript results in centrosome separation and cytokinesis defects. In mouse, the protein localizes to the nucleus of prophase I arrested oocytes and then becomes dispersed in meiotically competent oocytes. Knockdown of the protein delays exit from metaphase I and results in eggs with chromosomal abnormalities and elevated aneuploidy, demonstrating a function in regulation of meiosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015] Transcript Variant: This variant (1) represents the longer transcript. It encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200086 | Cdc14a (Myc-DDK-tagged ORF) - Rat CDC14 cell division cycle 14 homolog A (S. cerevisiae) (Cdc14a), transcript variant 1, (10 ug) |
CNY 7,700.00 |
|
| RR200086L3 | Lenti ORF clone of Cdc14a (Myc-DDK-tagged ORF) - Rat CDC14 cell division cycle 14 homolog A (S. cerevisiae) (Cdc14a), transcript variant 1, (10 ug) |
CNY 9,500.00 |
|
| RR200086L4 | Lenti ORF clone of Cdc14a (mGFP-tagged ORF) - Rat CDC14 cell division cycle 14 homolog A (S. cerevisiae) (Cdc14a), transcript variant 1, (10 ug) |
CNY 10,450.00 |
