Trpm1 (NM_001037733) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200070 representing NM_001037733 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCCATGAGGAAAATGAGCAGCTCCTTCAAGCGTGGTTCAATCAAGAGCTCCACATCAGGGTCCC AGAAGGGTCAGAAAGCATGGATAGAGAAGACGTTTTGCAAAAGGGAATGCATCTTCGTGATTCCTAGTAC AAAAGACCCGAACAGATGTTGCTGTGGTCAGCTCACTAACCAGCACATCCCCCCGCTGCCGAGTGTGACT CCCAGCTCAACAGCAGAGGACACCAAGCAAGGGGACGCACAGTCCGGGAAATGGTCTGTCAGCAAACACA CCCAGAGCTACCCAACAGACTCCTATGGGATTCTTGAATTCCAGGGTGGGGGCTACTCCAATAAAGCCAT GTATATCCGAGTCTCCTACGACACCAAGCCAGATTCTCTGCTCCATCTCATGGTGAAGGACTGGCAGCTG GAGCTCCCCAAGCTCTTGATATCTGTGCATGGAGGCCTCCAGAGCTTTGAGATGCAGCCCAAGCTGAAGC AGGTCTTTGGGAAAGGTCTGATCAAGGCTGCCATGACCACAGGGGCATGGATCTTCACCGGGGGAGTGAG CACTGGTGTCGTCAGCCATGTGGGGGATGCCTTGAAGGACCACTCCTCCAAGTCCAGAGGCCGGCTCTGT GCTATCGGAATCGCTCCCTGGGGCATGGTGGAGAACAAGGAAGATCTGGTTGGGAAAGATGTAACGAGAG TCTACCAGACCATGTCCAACCCTCTGAGCAAGCTCTCCGTGCTCAACAATTCTCACACTCACTTCATCTT GGCTGACAATGGCACCCTTGGCAAGTATGGTGCCGAGGTGAAGCTTCGAAGACAACTGGAAAAACACATC TCCCTGCAGAAGATCAACACGAGGCTGGGCCAGGGTGTACCTGTCGTGGGTCTCGTGGTGGAAGGTGGAC CTAATGTGGTTTCTATCGTCCTGGAGTATCTCAGAGAAGACCCTCCTGTCCCTGTGGTGGTTTGTGACGG CAGTGGACGCGCCTCCGACATTTTGTCTTTTGCACACAAATACTGCGACGAAGGAGGGGTCATAAACGAG TCCCTGCGGGACCAGCTTCTAGTTACCATTCAGAAAACATTTAATTACAGCAAGTCCCAGTCGCATCAGC TGTTTGCAATTATCATGGAGTGCATGAAGAAGAAAGAACTCGTCACTGTGTTTCGGATGGGCTCTGAGGG TCAGCAAGATGTTGAGATGGCAATTTTAACTGCCTTGCTCAAAGGAACCAACGTGTCAGCTCCAGATCAG CTGAGCTTGGCACTGGCTTGGAACCGCGTGGACATAGCACGAAGCCAGATCTTCGTCTTTGGCCCACACT GGCCGCCACTGGGAAGCCTGGCCCCTCCTGTGGACACCAAAGTCGCAGAGAAGGAGAAGAAGCCACCCAC AGCCACCACCAAGGGAAGAGGAAAAGGGAAAGGCAAGAAGAAAGGCAAAGTGAAAGAGGAGGTGGAGGAA GAAACAGACCCCCGGAAGATCGAGCTGCTGAACTGGGTGAACGCCCTGGAGCAAGCCATGCTGGACGCTC TTGTCTTAGACCGGGTTGACTTTGTGAAGCTCCTGATTGAAAACGGAGTGAACATGCAGCATTTCCTCAC TATCCCGAGGCTGGAGGAGCTTTACAACACTAGACTGGGCCCACCAAACACCCTTCATCTGCTGGTGCGG GATGTGAAGAAGAGCAACCTTCCACCTGACTACCACATCAGCCTCATCGATATAGGACTGGTGTTGGAGT ACCTCATGGGAGGTGCCTACCGCTGCAACTACACTCGGAAAAGCTTCCGGACTCTCTACAACAACTTGTT TGGCCCTAAGAGGCCAAAAGCACTTAAACTCCTGGGAATGGAAGATGATGAGCCCCCAGCCAAGGGGAAG AAGAAAAAGAAGAAGAAGAAAGAGGAGGAGATTGATATAGATGTGGACGACCCCGCTGTGAGTCGGTTCC AGTATCCGTTCCACGAGCTCATGGTATGGGCCGTGCTCATGAAGCGCCAGAAGATGGCAGTCTTCCTGTG GCAGCGTGGGGAGGAGTGCATGGCAAAGGCCTTGGTGGCCTGTAAGCTCTACAAGGCCATGGCCCACGAG TCCTCGGAGAGCGAGCTGGTGGATGACATCTCCCAGGATCTGGACAACAATTCCAAGGATTTTGGCCAGC TCGCTGTGGAGCTGTTAGACCAGTCTTACAAGCACGATGAGCAAGTGGCCATGAAACTTCTGACCTATGA GCTCAAGAACTGGAGCAACTCGACCTGCCTTAAGCTGGCCGTGGCGGCCAAGCACAGGGACTTTATCGCT CACACCTGCAGCCAGATGCTGCTGACAGACATGTGGATGGGAAGGCTTCGCATGAGGAAGAACCCTGGCC TGAAGGTCATCATGGGGATCCTTATCCCGCCCACCATCTTGTTTTTGGAATTCCGTTCATATGATGACTT CTCCTATCAAACATCCAAGGAGAATGAAGATGGCAAAGAAAAGGAAGAAGAGAATGTGGATGCAAATGCA GATGCTGGCTCAAGAAAGGGGGATGAGGAGAATGAGCACAAAAAACAGAGGAGCATTCCCATTGGAACAA AGATCTGTGAATTCTATAATGCGCCCATTGTCAAGTTCTGGTTTTACACAATCTCCTACTTGGGCTACTT GCTGCTGTTCAATTATGTCATCCTGGTGCGGATGGACGGCTGGCCTTCACCCCAAGAATGGATCGTTATC TCCTACATTGTGAGCCTGGCACTAGAGAAAATACGAGAGATCCTCATGTCGGAACCAGGCAAACTCAGCC AGAAAATCAAAGTGTGGCTCCAAGAGTACTGGAACATCACAGACCTTGTGGCCATTTCCATGTTCATGGT TGGGGCCATCCTTCGCCTCCAGAACCAGCCATACATGGGCTATGGCCGGGTCATCTACTGTGTGGATATC ATCCTCTGGTACATCCGAGTGTTAGACATCTTTGGTGTCAACAAGTATCTTGGCCCCTATGTAATGATGA TTGGAAAGATGATGATTGACATGCTGTACTTTGTGGTCATTATGCTGGTTGTGCTGATGAGTTTCGGCGT AGCTCGGCAAGCCATCTTGCACCCAGAGGAGAAGCCTTCTTGGAAACTGGCCCGAAACATCTTCTACATG CCCTACTGGATGATCTACGGAGAAGTGTTTGCAGACCAGATAGACCTCTATGCCATGGAAATCAACCCTC CTTGTGGTGAAAATCTCTATGATGAGGAAGGCAAGAGGCTCCCTCCCTGCATCCCTGGTGCCTGGCTCAC ACCTGCCCTCATGGCCTGTTATCTCCTGGTGGCCAATATCCTGCTGGTCAACCTGCTGATTGCTGTTTTC AACAATACCTTCTTTGAAGTCAAGTCAATATCCAATCAAGTGTGGAAGTTCCAGCGGTACCAACTGATCA TGACATTTCACGACAGGCCGGTCCTGCCCCCGCCAATGATCATCTTAAGCCACATCTACATCATCGTTAT GCGTCTCAGTGGTCGCTGCAGAAAAAAGAGAGAAGGGGACCAGGAGGAGCGGGATCGTGGGTTGAAGCTG TTCCTCAGCGACGAGGAACTGAAAAAACTGCATGAGTTTGAGGAGCAGTGCGTGCAGGAGCACTTCCGAG AAAAGGAGGATGAGCAGCAGTCGTCCAGCGATGAGCGCATCCGGGTTACAAGCGAGAGAGTTGAAAATAT GTCAATGAGGCTGGAGGAAATCAACGAAAGAGAAAATTTCATGAAGGCGTCTCTGCAGACTGTCGACCTT CGGCTCTCTCAGCTGGAGGAGCTATCTGGCAGGATGGTGGGCGCTCTGGAGAATCTGGCAGGCATCGACA GGTCTGATCTGATCCAGGCTCGATCAAGAGCATCCTCAGAATGTGAGGCCACCTACCTGCTGCGGCAGAG CAGTATCAACAGCGCCGATGGCTACAGCATGTACCGCTACCATTTCAATGGCGAGGAGCTTCTGTTTGAG GAGCCCGCCCTCTCCACTTCGCCAGGGACAGTGTTCCGGAAAAAAACCTGCTCCTTCCGCGTGAAGGAAG AGGATGTAAAACCACACCTGGACCAGCCAAGTAGCCTGCACCACACCCCAGGCCCCAGCCCGCCTGCCAC GCCAGGTCGCAGCCGGCTTGCCCTTGATGGTCCCTTGAGCACAGAGTTGAGACCTGGACTGGATCCTGGT ATCTCTGCGGGTGAGCTTGATCCGCGGGCAGATTTCAAGAGCGCCGAAGTTGCACCAAGTCTGAACACGG CGAATGTTGCAAGCACTCAGCTGACCGTCGAAAGCACTGTTTCCCACCCACTGCGGGAGAGCAAACTCGC GCGCTACTACCCCGGAGACCTCAACACCTACAAAACAATGAAGTCCAGAAGCTTCGTCTATTCTGAAGGA AGAAAACTTGTCCGAGGTCTTAGCAACTGGGGTGCCGAGTACAGTTCAATCATGGACCAAACATGGAACT CGGCAGAATGGAGATGTCAAGTTCAAAGGATCACACGCTCCCGCAGCACAGACATCCCGTACATTGTGTC TGAAGCAGCCTCCCAAGATGAGTTTGAGGATGAGCACAGAGAATCTCTTCTGGCTCCTCAGATCTCCCGT TCAGCCCTCACGGTCTCTGACAGGCCCGAAAAGGAAAACTTGCTGTCTGTGAAGCCACACCAGACTTTAG GATTCCCCTGCCTACGGTCAAGAAGTTTACATGGTCATCCTAGGAGTGCCAAACCCTCTCCTAGCAAATT AGACAGGGCGGGACATGCCAGCAGCACCAGCAACTTAGCAGTTATGTCAGACGCTCCAGAGGGACAAAAC ACCCAGCAGGAGAAAGGAAACCCGGAAACTGAATGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001037733
Insert Size	4869 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001037733.1, NP_001032822.1
RefSeq Size	5012 bp
RefSeq ORF	4869 bp
Locus ID	361586
UniProt ID	Q2WEA5
Gene Summary	Calcium channel which may play a role in metastasis suppression. May act as a spontaneously active, calcium-permeable plasma membrane channel (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1.

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