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Phospholipase C epsilon 1 (PLCE1) (NM_001288989) Human Tagged ORF Clone
CAT＃: RG240241
- TrueORF®
PLCE1 (tGFP-tagged) - Human phospholipase C, epsilon 1 (PLCE1), transcript variant 3
ORF Plasmid: DDK
"NM_001288989" in other vectors (2)
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CNY 19,370.00
货期*
2周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | NPHS3; PLCE; PPLC |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG240241 representing NM_001288989.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACTTCTGAAGAAATGACAGCTTCTGTTCTCATACCTGTGACTCAGAGAAAAGTGGTTTCTGCCCAG TCGGCTGCAGATGAAAGTAGTGAAAAGGTCTCAGACATCAATATTTCAAAAGCACATACTGTCAGACGA AGTGGGGAGACTTCTCATACCATCTCACAACTGAACAAACTTAAAGAAGAACCTTCTGGAAGCAACTTG CCAAAGATTCTCTCAATAGCGAGGGAGAAAATAGTGAGTGATGAGAACAGTAATGAAAAATGTTGGGAG AAAATCATGCCAGATTCTGCGAAAAACCTTAACATTAACTGCAACAACATATTGAGAAACCATCAGCAT GGCCTTCCTCAGAGACAATTTTATGAAATGTACAACTCTGTTGCTGAGGAAGACTTGTGTTTAGAAACT GGAATTCCTTCTCCACTGGAAAGAAAGGTGTTCCCTGGAATTCAACTGGAACTAGACAGACCTTCCATG GGCATTAGTCCTTTAGGAAATCAGTCAGTGATCATAGAGACAGGCAGAGCACACCCTGACAGCAGAAGG GCAGTATTTCATTTTCATTATGAAGTTGACAGAAGAATGTCAGACACTTTCTGTACCCTATCAGAAAAC TTAATTTTAGACGATTGTGGAAATTGTGTACCACTACCTGGGGGTGAGGAGAAGCAAAAGAAAAACTAT GTGGCATATACCTGTAAACTGATGGAATTGGCCAAAAATTGTGATAATAAGAATGAGCAGCTGCAGTGT GATCATTGTGACACCTTGAATGATAAATACTTTTGCTTTGAAGGCTCTTGTGAGAAGGTTGACATGGTA TATTCAGGTGATAGCTTTTGTAGGAAAGACTTTACTGACAGTCAAGCTGCCAAGACCTTTTTGAGCCAT TTTGAGGACTTCCCTGATAATTGTGATGATGTAGAAGAAGACGCTTTTAAAAGCAAAAAGGAGCGATCC ACTTTGTTAGTCAGGAGATTCTGTAAAAATGACAGAGAAGTTAAGAAATCTGTGTATACTGGAACAAGA GCAATTGTGAGAACTCTGCCTTCTGGCCACATTGGGCTGACTGCATGGAGTTACATAGATCAGAAGAGA AATGGTCCCTTACTGCCTTGTGGGAGAGTAATGGAACCCCCGTCAACAGTGGAGATAAGGCAAGATGGG AGCCAACGTCTGTCAGAAGCCCAGTGGTATCCTATCTACAATGCAGTGAGAAGAGAAGAAACAGAAAAT ACAGTTGGATCTCTACTCCATTTCCTCACCAAGCTCCCAGCCTCCGAGACAGCCCATGGAAGGATAAGC GTTGGTCCATGCTTAAAGCAATGTGTCCGAGACACTGTATGTGAGTATCGCGCCACCCTCCAAAGGACT TCAATATCGCAGTACATCACCGGTTCTCTCCTAGAAGCAACCACGTCTTTGGGAGCAAGAAGTGGCCTT CTCAGTACTTTTGGAGGATCCACTGGACGAATGATGCTGAAAGAACGCCAGCCAGGCCCCTCTGTGGCC AATTCCAATGCCCTCCCTTCAAGTTCAGCTGGGATCAGCAAGGAGCTGATCGATCTGCAGCCTCTCATC CAGTTCCCAGAGGAAGTCGCCAGCATCCTGATGGAGCAAGAGCAGACTATTTACCGCAGGGTCTTGCCA GTCGACTACCTTTGCTTCTTAACACGGGACTTGGGCACTCCTGAATGCCAGAGCTCCTTGCCCTGCCTC AAAGCATCCATCTCAGCGTCGATTCTTACCACTCAGAATGGAGAGCACAATGCCCTTGAAGATCTGGTG ATGAGGTTTAATGAGGTGAGCTCCTGGGTGACATGGCTGATCCTCACGGCAGGCTCCATGGAGGAGAAG CGAGAAGTCTTTTCATATTTGGTGCATGTGGCCAAATGCTGCTGGAACATGGGCAACTACAACGCTGTC ATGGAGTTCTTGGCTGGCCTCAGGTCAAGAAAAGTTTTAAAAATGTGGCAGTTCATGGACCAGTCTGAT ATTGAGACCATGAGGAGCCTGAAGGATGCTATGGCCCAGCATGAGTCCTCTTGTGAGTACAGAAAGGTG GTGACACGTGCCCTGCACATCCCTGGCTGTAAGGTGGTTCCATTCTGTGGGGTGTTTCTGAAGGAGCTC TGTGAAGTGCTTGACGGCGCCTCCGGTCTCATGAAGCTTTGCCCGCGGTACAATTCCCAAGAAGAAACT TTAGAGTTTGTAGCAGATTACAGTGGACAAGATAATTTCTTACAACGAGTGGGACAAAATGGCTTAAAG AATTCGGAGAAGGAGTCCACTGTCAACAGCATCTTTCAGGTCATCCGGAGCTGCAATCGAAGTCTGGAG ACAGACGAGGAGGACAGCCCCAGTGAAGGAAACAGCTCCAGGAAAAGCTCCTTGAAGGATAAAAGCCGA TGGCAGTTTATAATTGGAGATTTGTTGGATTCAGACAATGACATCTTTGAGCAATCCAAAGAATACGAC TCTCATGGTTCAGAGGACTCACAGAAGGCCTTCGACCATGGGACGGAGCTCATCCCTTGGTACGTGCTG TCCATCCAAGCCGATGTGCACCAGTTCCTGCTGCAGGGGGCCACGGTCATCCACTACGACCAGGACACA CACCTCTCTGCCCGCTGCTTCCTCCAGCTTCAGCCCGACAATAGCACCTTGACCTGGGTAAAGCCCACA ACTGCCTCCCCAGCCAGCAGTAAAGCAAAACTTGGTGTACTTAATAACACAGCTGAGCCTGGAAAATTC CCACTACTGGGTAATGCTGGATTAAGTAGCCTGACGGAAGGGGTCTTGGATCTTTTTGCAGTGAAGGCT GTATACATGGGCCACCCTGGCATTGATATACACACTGTGTGTGTTCAGAACAAACTGGGTAGCATGTTC CTGTCAGAGACTGGTGTGACATTGCTCTATGGGCTTCAGACCACAGACAACAGATTATTGCACTTCGTG GCACCAAAGCACACAGCTAAAATGCTCTTCAGCGGATTATTGGAACTCACTAGAGCTGTGAGAAAGATG AGGAAATTCCCTGACCAAAGACAGCAGTGGCTGCGGAAACAGTACGTCAGCCTTTATCAGGAGGATGGA CGGTATGAAGGCCCAACTTTGGCTCACGCTGTGGAGTTGTTTGGTGGCAGACGGTGGAGTGCTCGAAAC CCCAGCCCCGGAACATCAGCAAAGAATGCTGAGAAGCCCAATATGCAGAGAAACAATACCCTGGGCATA AGCACTACCAAGAAAAAGAAGAAAATCCTCATGAGGGGTGAGAGTGGAGAGGTAACTGACGATGAGATG GCAACCCGAAAGGCCAAGATGCACAAAGAGTGTCGAAGCCGGAGTGGTTCTGATCCTCAAGACATTAAT GAACAAGAAGAATCAGAGGTGAATGCCATCGCTAACCCTCCAAACCCCCTCCCTTCCAGAAGAGCCCAC TCTTTGACCACAGCTGGGTCCCCCAACTTGGCTGCCGGGACGTCATCTCCCATCAGTGCTTGGAGCAGT AGTAGCTGGCACGGGCGGATCAAAGGCGGCATGAAGGGATTTCAGAGCTTCATGGTTTCAGATAGCAAC ATGAGTTTTGTTGAATTTGTTGAGCTGTTCAAATCATTCAGTGTCAGGAGCCGCAAGGACCTGAAGGAT CTGTTTGATGTCTATGCAGTGCCCTGCAACCGATCTGGCTCCGAGTCAGCCCCACTCTACACCAACCTG ACAATTGATGAAAACACCAGCGATCTTCAGCCTGACCTAGATCTGTTGACCAGAAATGTCTCGGATTTG GGGTTGTTCATTAAGAGTAAACAGCAGCTATCGGACAACCAGAGGCAGATATCTGATGCCATTGCTGCT GCAAGCATTGTGACAAATGGCACTGGGATTGAGAGCACATCTCTGGGCATTTTTGGGGTGGGCATACTT CAGCTCAACGATTTCCTCGTGAATTGCCAAGGAGAACACTGCACTTATGATGAAATCCTCAGCATCATC CAGAAGTTCGAGCCTAGCATCAGTATGTGTCATCAGGGACTAATGTCATTTGAAGGGTTTGCCAGGTTT CTGATGGATAAAGAAAATTTTGCCTCAAAAAATGATGAGTCACAGGAGAACATTAAAGAACTGCAGCTA CCCCTCTCATACTATTACATCGAATCTTCGCACAATACCTACCTCACGGGCCATCAGCTCAAAGGAGAA TCCTCGGTAGAACTCTACAGCCAGGTCCTTTTGCAAGGCTGTCGAAGTGTAGAATTGGACTGCTGGGAC GGAGACGATGGGATGCCCATCATTTATCATGGACATACGCTGACAACCAAGATCCCCTTCAAGGAAGTG GTTGAAGCCATTGATCGCAGTGCCTTCATCAACTCTGACCTGCCAATCATCATATCGATTGAGAACCAC TGTTCATTGCCTCAGCAACGAAAAATGGCAGAAATTTTCAAGACTGTGTTTGGAGAAAAGCTGGTGACT AAATTCTTATTTGAGACTGATTTCTCAGATGATCCAATGCTTCCTTCACCTGACCAACTCAGAAAGAAA GTTCTTCTTAAAAACAAGAAGCTAAAAGCCCATCAGACGCCAGTGGATATCTTAAAGCAAAAGGCTCAT CAGTTAGCATCTATGCAAGTGCAGGCTTATAATGGTGGGAATGCCAACCCCCGACCTGCCAATAATGAG GAAGAGGAAGATGAGGAGGACGAATATGATTATGACTATGAATCCCTTTCTGATGACAACATTCTGGAA GACAGACCTGAAAATAAATCATGTAATGACAAGCTTCAGTTTGAATATAATGAAGAAATCCCAAAGAGG ATAAAGAAAGCAGATAACTCTGCTTGCAACAAAGGAAAGGTTTATGATATGGAACTGGGAGAAGAATTT TATCTTGATCAGAATAAAAAGGAAAGCAGACAGATTGCACCAGAGCTTTCTGACCTTGTAATCTATTGT CAAGCAGTAAAATTTCCAGGACTGTCAACTCTAAATGCATCTGGCTCTAGCAGAGGAAAAGAAAGGAAA AGCAGGAAGTCCATTTTTGGCAACAATCCGGGCAGAATGAGCCCAGGGGAGACAGCATCATTTAACAAA ACATCTGGAAAAAGTTCCTGTGAAGGCATTCGACAGACCTGGGAGGAATCTTCTTCCCCTCTCAACCCA ACCACGTCCCTCAGTGCTATCATTAGAACTCCCAAATGTTATCATATCTCGTCGCTGAATGAAAATGCC GCCAAACGTCTGTGTCGCAGGTATTCTCAGAAACTGACCCAGCACACCGCCTGTCAGCTGCTGAGAACT TACCCTGCTGCCACCCGCATCGACTCTTCCAACCCGAACCCCCTCATGTTCTGGCTCCATGGGATACAG CTTGTGGCACTCAACTACCAGACTGATGATCTCCCTTTACATTTAAATGCTGCAATGTTTGAGGCAAAT GGTGGTTGTGGTTATGTATTGAAACCTCCAGTTCTGTGGGACAAGAACTGCCCCATGTATCAGAAGTTT TCTCCACTAGAAAGAGATCTGGACAGCATGGATCCTGCAGTCTATTCTTTAACTATTGTCTCTGGTCAG AATGTGTGCCCCAGTAATAGCATGGGAAGCCCGTGCATTGAAGTCGACGTCCTGGGCATGCCTCTGGAC AGCTGCCATTTCCGCACAAAGCCCATCCATCGAAACACCCTGAACCCCATGTGGAACGAGCAGTTTCTG TTCCACGTTCACTTCGAAGATCTTGTATTTCTTCGTTTTGCAGTTGTGGAAAACAATAGTTCAGCGGTA ACTGCTCAGAGAATCATTCCACTGAAAGCTTTAAAACGAGGATATCGACATCTTCAGCTGCGAAACCTT CACAATGAAGTCTTGGAGATTTCTAGTTTATTCATTAACAGCAGAAGGATGGAAGAAAATTCCTCTGGC AATACCATGTCAGCCTCTTCGATGTTTAATACAGAAGAAAGAAAATGTTTGCAGACTCACAGAGTCACG GTGCATGGGGTCCCAGGGCCAGAGCCCTTTACCGTTTTCACTATTAATGGAGGCACCAAGGCAAAGCAG CTTCTGCAGCAAATTCTGACAAATGAACAAGACATCAAACCTGTTACCACAGACTATTTTTTGATGGAA GAAAAATATTTTATATCTAAAGAAAAGAATGAATGTAGGAAACAACCATTCCAGAGAGCCATTGGTCCA GAAGAGGAGATCATGCAAATTTTAAGCAGCTGGTTTCCAGAAGAGGGATACATGGGCAGGATTGTCTTA AAAACCCAGCAGGAAAACCTAGAAGAGAAAAACATTGTTCAAGATGACAAAGAGGTGATCTTGAGCTCA GAGGAGGAGAGTTTCTTTGTCCAAGTGCATGATGTTTCTCCAGAGCAACCTCGAACAGTCATCAAAGCA CCCCGCGTCAGCACTGCACAGGATGTCATTCAGCAGACCTTATGCAAAGCCAAATATTCCTACAGCATC CTGAGCAACCCCAATCCAAGCGACTATGTGCTTTTGGAAGAGGTGGTGAAAGACACTACCAACAAGAAG ACTACCACACCAAAGTCCTCTCAGCGGGTCCTTCTGGATCAGGAGTGTGTGTTTCAAGCCCAAAGCAAG TGGAAAGGTGCAGGAAAATTCATCCTTAAGCTAAAGGAGCAGGTGCAGGCATCTCGAGAAGATAAAAAG AAAGGCATTTCTTTCGCAAGTGAACTCAAGAAGCTCACCAAGTCAACTAAACAGCCCCGAGGACTTACA TCACCTTCTCAGCTCTTGACCTCAGAAAGTATCCAAACCAAGGAGGAGAAACCTGTGGGTGGCTTGTCC TCCAGTGACACAATGGATTACCGACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG240241
Blue=ORF Red=Cloning site Green=Tag(s) MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNL PKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLET GIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSEN LILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMV YSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTR AIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETEN TVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGL LSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLP VDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEK REVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKV VTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLK NSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYD SHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPT TASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMF LSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDG RYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEM ATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPISAWSS SSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNL TIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGIL QLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQL PLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEV VEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKK VLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDNILE DRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYC QAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNP TTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQ LVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQ NVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAV TAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVT VHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGP EEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKA PRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSK WKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLS SSDTMDYRQ TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001288989 |
| ORF Size | 6858 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001288989.2 |
| RefSeq Size | 7957 bp |
| RefSeq ORF | 6861 bp |
| Locus ID | 51196 |
| UniProt ID | Q9P212 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| MW | 257.6 kDa |
| Gene Summary | This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009] |
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