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NuMA (NUMA1) (NM_001286561) Human Tagged ORF Clone
CAT＃: RG240233
- TrueORF®
NUMA1 (tGFP-tagged) - Human nuclear mitotic apparatus protein 1 (NUMA1), transcript variant 2
ORF Plasmid: DDK
"NM_001286561" in other vectors (2)
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CNY 19,370.00
货期*
2周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | NMP-22; NUMA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG240233 representing NM_001286561.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACACTCCACGCCACCCGGGGGGCTGCACTCCTCTCTTGGGTGAACAGTCTACACGTGGCTGACCCT GTGGAGGCTGTGCTGCAGCTCCAGGACTGCAGCATCTTCATCAAGATCATTGACAGAATCCATGGCACT GAAGAGGGACAGCAAATCTTGAAGCAGCCGGTGTCAGAGAGACTGGACTTTGTGTGCAGTTTTCTGCAG AAAAATCGAAAACATCCCTCTTCCCCAGAATGCCTGGTATCTGCACAGAAGGTGCTAGAGGGATCAGAG CTGGAACTGGCGAAGATGACCATGCTGCTCTTATACCACTCTACCATGAGCTCCAAAAGTCCCAGGGAC TGGGAACAGTTTGAATATAAAATTCAGGCTGAGTTGGCTGTCATTCTTAAATTTGTGCTGGACCATGAG GACGGGCTAAACCTTAATGAGGACCTAGAGAACTTCCTACAGAAAGCTCCTGTGCCTTCTACCTGTTCT AGCACATTCCCTGAAGAGCTCTCCCCACCTAGCCACCAGGCCAAGAGGGAGATTCGCTTCCTAGAGCTA CAGAAGGTTGCCTCCTCTTCCAGTGGGAACAACTTTCTCTCAGGTTCTCCAGCTTCTCCCATGGGTGAT ATCCTGCAGACCCCACAGTTCCAGATGAGACGGCTGAAGAAGCAGCTTGCTGATGAGAGAAGTAATAGG GATGAGCTGGAGCTGGAGCTAGCTGAGAACCGCAAGCTCCTCACCGAGAAGGATGCACAGATAGCCATG ATGCAGCAGCGCATTGACCGCCTAGCCCTGCTGAATGAGAAGCAGGCGGCCAGCCCACTGGAGCCCAAG GAGCTTGAGGAGCTGCGTGACAAGAATGAGAGCCTTACCATGCGGCTGCATGAAACCCTGAAGCAGTGC CAGGACCTGAAGACAGAGAAGAGCCAGATGGATCGCAAAATCAACCAGCTTTCGGAGGAGAATGGAGAC CTTTCCTTTAAGCTGCGGGAGTTTGCCAGTCATCTGCAGCAGCTACAGGATGCCCTCAATGAGCTGACG GAGGAGCACAGCAAGGCCACTCAGGAGTGGCTAGAGAAGCAGGCCCAGCTGGAGAAGGAGCTCAGCGCA GCCCTGCAGGACAAGAAATGCCTTGAAGAGAAGAACGAAATCCTTCAGGGAAAACTTTCACAGCTGGAA GAACACTTGTCCCAGCTGCAGGATAACCCACCCCAGGAGAAGGGCGAGGTGCTGGGTGATGTCTTGCAG CTGGAAACCTTGAAGCAAGAGGCAGCCACTCTTGCTGCAAACAACACACAGCTCCAAGCCAGGGTAGAG ATGCTGGAGACTGAGCGAGGCCAGCAGGAAGCCAAGCTGCTTGCTGAGCGGGGCCACTTCGAAGAAGAA AAGCAGCAGCTGTCTAGCCTGATCACTGACCTGCAGAGCTCCATCTCCAACCTCAGCCAGGCCAAGGAA GAGCTGGAGCAGGCCTCCCAGGCTCATGGGGCCCGGTTGACTGCCCAGGTGGCCTCTCTGACCTCTGAG CTCACCACACTCAATGCCACCATCCAGCAACAGGATCAAGAACTGGCTGGCCTGAAGCAGCAGGCCAAA GAGAAGCAGGCCCAGCTAGCACAGACCCTCCAACAGCAAGAACAGGCCTCCCAGGGCCTCCGCCACCAG GTGGAGCAGCTAAGCAGTAGCCTGAAGCAGAAGGAGCAGCAGTTGAAGGAGGTAGCGGAGAAGCAGGAG GCAACTAGGCAGGACCATGCCCAGCAACTGGCCACTGCTGCAGAGGAGCGAGAGGCCTCCTTAAGGGAG CGGGATGCGGCTCTCAAGCAGCTGGAGGCACTGGAGAAGGAGAAGGCTGCCAAGCTGGAGATTCTGCAG CAGCAACTTCAGGTGGCTAATGAAGCCCGGGACAGTGCCCAGACCTCAGTGACACAGGCCCAGCGGGAG AAGGCAGAGCTGAGCCGGAAGGTGGAGGAACTCCAGGCCTGTGTTGAGACAGCCCGCCAGGAACAGCAT GAGGCCCAGGCCCAGGTTGCAGAGCTAGAGTTGCAGCTGCGGTCTGAGCAGCAAAAAGCAACTGAGAAA GAAAGGGTGGCCCAGGAGAAGGACCAGCTCCAGGAGCAGCTCCAGGCCCTCAAAGAGTCCTTGAAGGTC ACCAAGGGCAGCCTTGAAGAGGAGAAGCGCAGGGCTGCAGATGCCCTGGAAGAGCAGCAGCGTTGTATC TCTGAGCTGAAGGCAGAGACCCGAAGCCTGGTGGAGCAGCATAAGCGGGAACGAAAGGAGCTGGAAGAA GAGAGGGCTGGGCGCAAGGGGCTGGAGGCTCGATTACAGCAGCTTGGGGAGGCCCATCAGGCTGAGACT GAAGTCCTGCGGCGGGAGCTGGCAGAGGCCATGGCTGCCCAGCACACAGCTGAGAGTGAGTGTGAGCAG CTCGTCAAAGAAGTAGCTGCCTGGCGTGAGCGGTATGAGGATAGCCAGCAAGAGGAGGCACAGTATGGC GCCATGTTCCAGGAACAGCTGATGACTTTGAAGGAGGAATGTGAGAAGGCCCGCCAGGAGCTGCAGGAG GCAAAGGAGAAGGTGGCAGGCATAGAATCCCACAGCGAGCTCCAGATAAGCCGGCAGCAGAACGAACTA GCTGAGCTCCATGCCAACCTGGCCAGAGCACTCCAGCAGGTCCAAGAGAAGGAAGTCAGGGCCCAGAAG CTTGCAGATGACCTCTCCACTCTGCAGGAAAAGATGGCTGCCACCAGCAAAGAGGTGGCCCGCTTGGAG ACCTTGGTGCGCAAGGCAGGTGAGCAGCAGGAAACAGCCTCCCGGGAGTTAGTCAAGGAGCCTGCGAGG GCAGGAGACAGACAGCCCGAGTGGCTGGAAGAGCAACAGGGACGCCAGTTCTGCAGCACACAGGCAGCG CTGCAGGCTATGGAGCGGGAGGCAGAGCAGATGGGCAATGAGCTGGAACGGCTGCGGGCCGCGCTGATG GAGAGCCAGGGGCAGCAGCAGGAGGAGCGTGGGCAGCAGGAAAGGGAGGTGGCGCGGCTGACCCAGGAG CGGGGCCGTGCCCAGGCTGACCTTGCCCTGGAGAAGGCGGCCAGAGCAGAGCTTGAGATGCGGCTGCAG AACGCCCTCAACGAGCAGCGTGTGGAGTTCGCTACCCTGCAAGAGGCACTGGCTCATGCCCTGACGGAA AAGGAAGGCAAGGACCAGGAGTTGGCCAAGCTTCGTGGTCTGGAGGCAGCCCAGATAAAAGAGCTGGAG GAACTTCGGCAAACCGTGAAGCAACTGAAGGAACAGCTGGCTAAGAAAGAAAAGGAGCACGCATCTGGC TCAGGAGCCCAATCTGAGGCTGCTGGCAGGACAGAGCCAACAGGCCCCAAGCTGGAGGCACTGCGGGCA GAGGTGAGCAAGCTGGAACAGCAATGCCAGAAGCAGCAGGAGCAGGCTGACAGCCTGGAACGCAGCCTC GAGGCTGAGCGGGCCTCCCGGGCTGAGCGGGACAGTGCTCTGGAGACTCTGCAGGGCCAGTTAGAGGAG AAGGCCCAGGAGCTAGGGCACAGTCAGAGTGCCTTAGCCTCGGCCCAACGGGAGTTGGCTGCCTTCCGC ACCAAGGTACAAGACCACAGCAAGGCTGAAGATGAGTGGAAGGCCCAGGTGGCCCGGGGCCGGCAAGAG GCTGAGAGGAAAAATAGCCTCATCAGCAGCTTGGAGGAGGAGGTGTCCATCCTGAATCGCCAGGTCCTG GAGAAGGAGGGGGAGAGCAAGGAGTTGAAGCGGCTGGTGATGGCCGAGTCAGAGAAGAGCCAGAAGCTG GAGGAGAGGCTGCGCCTGCTGCAGGCAGAGACAGCCAGCAACAGTGCCAGAGCTGCAGAACGCAGCTCT GCTCTGCGGGAGGAGGTGCAGAGCCTCCGGGAGGAGGCTGAGAAACAGCGGGTGGCTTCAGAGAACCTG CGGCAGGAGCTGACCTCACAGGCTGAGCGTGCGGAGGAGCTGGGCCAAGAATTGAAGGCGTGGCAGGAG AAGTTCTTCCAGAAAGAGCAGGCCCTCTCCACCCTGCAGCTCGAGCACACCAGCACACAGGCCCTGGTG AGTGAGCTGCTGCCAGCTAAGCACCTCTGCCAGCAGCTGCAGGCCGAGCAGGCCGCTGCCGAGAAACGC CACCGTGAGGAGCTGGAGCAGAGCAAGCAGGCCGCTGGGGGACTGCGGGCAGAGCTGCTGCGGGCCCAG CGGGAGCTTGGGGAGCTGATTCCTCTGCGGCAGAAGGTGGCAGAGCAGGAGCGAACAGCTCAGCAGCTG CGGGCAGAGAAGGCCAGCTATGCAGAGCAGCTGAGCATGCTGAAGAAGGCGCATGGCCTGCTGGCAGAG GAGAACCGGGGGCTGGGTGAGCGGGCCAACCTTGGCCGGCAGTTTCTGGAAGTGGAGTTGGACCAGGCC CGGGAGAAGTATGTCCAAGAGTTGGCAGCCGTACGTGCTGATGCTGAGACCCGTCTGGCTGAGGTGCAG CGAGAAGCACAGAGCACTGCCCGGGAGCTGGAGGTGATGACTGCCAAGTATGAGGGTGCCAAGGTCAAG GTCCTGGAGGAGAGGCAGCGGTTCCAGGAAGAGAGGCAGAAACTCACTGCCCAGGTGGAAGAACTGAGT AAGAAACTGGCTGACTCTGACCAAGCCAGCAAGGTGCAGCAGCAGAAGCTGAAGGCTGTCCAGGCTCAG GGAGGCGAGAGCCAGCAGGAGGCCCAGCGCCTCCAGGCCCAGCTGAATGAACTGCAAGCCCAGTTGAGC CAGAAGGAGCAGGCAGCTGAGCACTATAAGCTGCAGATGGAGAAAGCCAAAACACATTATGATGCCAAG AAGCAGCAGAACCAAGAGCTGCAGGAGCAGCTGCGGAGCCTGGAGCAGCTGCAGAAGGAAAACAAAGAG CTGCGAGCTGAAGCTGAACGGCTGGGCCATGAGCTACAGCAGGCTGGGCTGAAGACCAAGGAGGCTGAA CAGACCTGCCGCCACCTTACTGCCCAGGTGCGCAGCCTGGAGGCACAGGTTGCCCATGCAGACCAGCAG CTTCGAGACCTGGGCAAATTCCAGGTGGCAACTGATGCTTTAAAGAGCCGTGAGCCCCAGGCTAAGCCC CAGCTGGACTTGAGTATTGACAGCCTGGATCTGAGCTGCGAGGAGGGGACCCCACTCAGTATCACCAGC AAGCTGCCTCGTACCCAGCCAGACGGCACCAGCGTCCCTGGAGAACCAGCCTCACCTATCTCCCAGCGC CTGCCCCCCAAGGTAGAATCCCTGGAGAGTCTCTACTTCACTCCCATCCCTGCTCGGAGTCAGGCCCCC CTGGAGAGCAGCCTGGACTCCCTGGGAGACGTCTTCCTGGACTCGGGTCGTAAGACCCGCTCCGCTCGT CGGCGCACCACGCAGATCATCAACATCACCATGACCAAGAAGCTAGATGTGGAAGAGCCAGACAGCGCC AACTCATCGTTCTACAGCACGCGGTCTGCTCCTGCTTCCCAGGCTAGCCTGCGAGCCACCTCCTCTACT CAGTCTCTAGCTCGCCTGGGTTCTCCCGATTATGGCAACTCAGCCCTGCTCAGCTTGCCTGGCTACCGC CCCACCACTCGCAGTTCTGCTCGTCGTTCCCAGGCCGGGGTGTCCAGTGGGGCCCCTCCAGGAAGGAAC AGCTTCTACATGGGCACTTGCCAGGATGAGCCTGAGCAGCTGGATGACTGGAACCGCATTGCAGAGCTG CAGCAGCGCAATCGAGTGTGCCCCCCACATCTGAAGACCTGCTATCCCCTGGAGTCCAGGCCTTCCCTG AGCCTGGGCACCATCACAGATGAGGAGATGAAAACTGGAGACCCCCAAGAGACCCTGCGCCGAGCCAGC ATGCAGCCAATCCAGATAGCCGAGGGCACTGGCATCACCACCCGGCAGCAGCGCAAACGGGTCTCCCTA GAGCCCCACCAGGGCCCTGGAACTCCTGAGTCTAAGAAGGCCACCAGCTGTTTCCCACGCCCCATGACT CCCCGAGACCGACATGAAGGGCGCAAACAGAGCACTACTGAGGCCCAGAAGAAAGCAGCTCCAGCTTCT ACTAAACAGGCTGACCGGCGCCAGTCGATGGCCTTCAGCATCCTCAACACACCCAAGAAGCTAGGGAAC AGCCTTCTGCGGCGGGGAGCCTCAAAGAAGGCCCTGTCCAAGGCTTCCCCCAACACTCGCAGTGGAACC CGCCGTTCTCCGCGCATTGCCACCACCACAGCCAGCGCCGCCACTGCTGCCGCCATTGGTGCCACCCCT CGAGCCAAGGGCAAGGCAAAGCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG240233
Blue=ORF Red=Cloning site Green=Tag(s) MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQ KNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHE DGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGD ILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPK ELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELT EEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQ LETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKE ELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQ VEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQ QQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEK ERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEE ERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYG AMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQK LADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAA LQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQ NALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASG SGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEE KAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVL EKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENL RQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKR HREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAE ENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVK VLEERQRFQEERQKLTAQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLS QKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAE QTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITS KLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSAR RRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYR PTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSL SLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMT PRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGT RRSPRIATTTASAATAAAIGATPRAKGKAKH TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001286561 |
| ORF Size | 6303 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001286561.1, NP_001273490.1 |
| RefSeq Size | 7431 bp |
| RefSeq ORF | 6306 bp |
| Locus ID | 4926 |
| UniProt ID | Q14980 |
| MW | 237 kDa |
| Gene Summary | This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013] |
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