NuMA (NUMA1) (NM_001286561) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NMP-22; NUMA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240233 representing NM_001286561 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACTCCACGCCACCCGGGGGGCTGCACTCCTCTCTTGGGTGAACAGTCTACACGTGGCTGACCCTG TGGAGGCTGTGCTGCAGCTCCAGGACTGCAGCATCTTCATCAAGATCATTGACAGAATCCATGGCACTGA AGAGGGACAGCAAATCTTGAAGCAGCCGGTGTCAGAGAGACTGGACTTTGTGTGCAGTTTTCTGCAGAAA AATCGAAAACATCCCTCTTCCCCAGAATGCCTGGTATCTGCACAGAAGGTGCTAGAGGGATCAGAGCTGG AACTGGCGAAGATGACCATGCTGCTCTTATACCACTCTACCATGAGCTCCAAAAGTCCCAGGGACTGGGA ACAGTTTGAATATAAAATTCAGGCTGAGTTGGCTGTCATTCTTAAATTTGTGCTGGACCATGAGGACGGG CTAAACCTTAATGAGGACCTAGAGAACTTCCTACAGAAAGCTCCTGTGCCTTCTACCTGTTCTAGCACAT TCCCTGAAGAGCTCTCCCCACCTAGCCACCAGGCCAAGAGGGAGATTCGCTTCCTAGAGCTACAGAAGGT TGCCTCCTCTTCCAGTGGGAACAACTTTCTCTCAGGTTCTCCAGCTTCTCCCATGGGTGATATCCTGCAG ACCCCACAGTTCCAGATGAGACGGCTGAAGAAGCAGCTTGCTGATGAGAGAAGTAATAGGGATGAGCTGG AGCTGGAGCTAGCTGAGAACCGCAAGCTCCTCACCGAGAAGGATGCACAGATAGCCATGATGCAGCAGCG CATTGACCGCCTAGCCCTGCTGAATGAGAAGCAGGCGGCCAGCCCACTGGAGCCCAAGGAGCTTGAGGAG CTGCGTGACAAGAATGAGAGCCTTACCATGCGGCTGCATGAAACCCTGAAGCAGTGCCAGGACCTGAAGA CAGAGAAGAGCCAGATGGATCGCAAAATCAACCAGCTTTCGGAGGAGAATGGAGACCTTTCCTTTAAGCT GCGGGAGTTTGCCAGTCATCTGCAGCAGCTACAGGATGCCCTCAATGAGCTGACGGAGGAGCACAGCAAG GCCACTCAGGAGTGGCTAGAGAAGCAGGCCCAGCTGGAGAAGGAGCTCAGCGCAGCCCTGCAGGACAAGA AATGCCTTGAAGAGAAGAACGAAATCCTTCAGGGAAAACTTTCACAGCTGGAAGAACACTTGTCCCAGCT GCAGGATAACCCACCCCAGGAGAAGGGCGAGGTGCTGGGTGATGTCTTGCAGCTGGAAACCTTGAAGCAA GAGGCAGCCACTCTTGCTGCAAACAACACACAGCTCCAAGCCAGGGTAGAGATGCTGGAGACTGAGCGAG GCCAGCAGGAAGCCAAGCTGCTTGCTGAGCGGGGCCACTTCGAAGAAGAAAAGCAGCAGCTGTCTAGCCT GATCACTGACCTGCAGAGCTCCATCTCCAACCTCAGCCAGGCCAAGGAAGAGCTGGAGCAGGCCTCCCAG GCTCATGGGGCCCGGTTGACTGCCCAGGTGGCCTCTCTGACCTCTGAGCTCACCACACTCAATGCCACCA TCCAGCAACAGGATCAAGAACTGGCTGGCCTGAAGCAGCAGGCCAAAGAGAAGCAGGCCCAGCTAGCACA GACCCTCCAACAGCAAGAACAGGCCTCCCAGGGCCTCCGCCACCAGGTGGAGCAGCTAAGCAGTAGCCTG AAGCAGAAGGAGCAGCAGTTGAAGGAGGTAGCGGAGAAGCAGGAGGCAACTAGGCAGGACCATGCCCAGC AACTGGCCACTGCTGCAGAGGAGCGAGAGGCCTCCTTAAGGGAGCGGGATGCGGCTCTCAAGCAGCTGGA GGCACTGGAGAAGGAGAAGGCTGCCAAGCTGGAGATTCTGCAGCAGCAACTTCAGGTGGCTAATGAAGCC CGGGACAGTGCCCAGACCTCAGTGACACAGGCCCAGCGGGAGAAGGCAGAGCTGAGCCGGAAGGTGGAGG AACTCCAGGCCTGTGTTGAGACAGCCCGCCAGGAACAGCATGAGGCCCAGGCCCAGGTTGCAGAGCTAGA GTTGCAGCTGCGGTCTGAGCAGCAAAAAGCAACTGAGAAAGAAAGGGTGGCCCAGGAGAAGGACCAGCTC CAGGAGCAGCTCCAGGCCCTCAAAGAGTCCTTGAAGGTCACCAAGGGCAGCCTTGAAGAGGAGAAGCGCA GGGCTGCAGATGCCCTGGAAGAGCAGCAGCGTTGTATCTCTGAGCTGAAGGCAGAGACCCGAAGCCTGGT GGAGCAGCATAAGCGGGAACGAAAGGAGCTGGAAGAAGAGAGGGCTGGGCGCAAGGGGCTGGAGGCTCGA TTACAGCAGCTTGGGGAGGCCCATCAGGCTGAGACTGAAGTCCTGCGGCGGGAGCTGGCAGAGGCCATGG CTGCCCAGCACACAGCTGAGAGTGAGTGTGAGCAGCTCGTCAAAGAAGTAGCTGCCTGGCGTGAGCGGTA TGAGGATAGCCAGCAAGAGGAGGCACAGTATGGCGCCATGTTCCAGGAACAGCTGATGACTTTGAAGGAG GAATGTGAGAAGGCCCGCCAGGAGCTGCAGGAGGCAAAGGAGAAGGTGGCAGGCATAGAATCCCACAGCG AGCTCCAGATAAGCCGGCAGCAGAACGAACTAGCTGAGCTCCATGCCAACCTGGCCAGAGCACTCCAGCA GGTCCAAGAGAAGGAAGTCAGGGCCCAGAAGCTTGCAGATGACCTCTCCACTCTGCAGGAAAAGATGGCT GCCACCAGCAAAGAGGTGGCCCGCTTGGAGACCTTGGTGCGCAAGGCAGGTGAGCAGCAGGAAACAGCCT CCCGGGAGTTAGTCAAGGAGCCTGCGAGGGCAGGAGACAGACAGCCCGAGTGGCTGGAAGAGCAACAGGG ACGCCAGTTCTGCAGCACACAGGCAGCGCTGCAGGCTATGGAGCGGGAGGCAGAGCAGATGGGCAATGAG CTGGAACGGCTGCGGGCCGCGCTGATGGAGAGCCAGGGGCAGCAGCAGGAGGAGCGTGGGCAGCAGGAAA GGGAGGTGGCGCGGCTGACCCAGGAGCGGGGCCGTGCCCAGGCTGACCTTGCCCTGGAGAAGGCGGCCAG AGCAGAGCTTGAGATGCGGCTGCAGAACGCCCTCAACGAGCAGCGTGTGGAGTTCGCTACCCTGCAAGAG GCACTGGCTCATGCCCTGACGGAAAAGGAAGGCAAGGACCAGGAGTTGGCCAAGCTTCGTGGTCTGGAGG CAGCCCAGATAAAAGAGCTGGAGGAACTTCGGCAAACCGTGAAGCAACTGAAGGAACAGCTGGCTAAGAA AGAAAAGGAGCACGCATCTGGCTCAGGAGCCCAATCTGAGGCTGCTGGCAGGACAGAGCCAACAGGCCCC AAGCTGGAGGCACTGCGGGCAGAGGTGAGCAAGCTGGAACAGCAATGCCAGAAGCAGCAGGAGCAGGCTG ACAGCCTGGAACGCAGCCTCGAGGCTGAGCGGGCCTCCCGGGCTGAGCGGGACAGTGCTCTGGAGACTCT GCAGGGCCAGTTAGAGGAGAAGGCCCAGGAGCTAGGGCACAGTCAGAGTGCCTTAGCCTCGGCCCAACGG GAGTTGGCTGCCTTCCGCACCAAGGTACAAGACCACAGCAAGGCTGAAGATGAGTGGAAGGCCCAGGTGG CCCGGGGCCGGCAAGAGGCTGAGAGGAAAAATAGCCTCATCAGCAGCTTGGAGGAGGAGGTGTCCATCCT GAATCGCCAGGTCCTGGAGAAGGAGGGGGAGAGCAAGGAGTTGAAGCGGCTGGTGATGGCCGAGTCAGAG AAGAGCCAGAAGCTGGAGGAGAGGCTGCGCCTGCTGCAGGCAGAGACAGCCAGCAACAGTGCCAGAGCTG CAGAACGCAGCTCTGCTCTGCGGGAGGAGGTGCAGAGCCTCCGGGAGGAGGCTGAGAAACAGCGGGTGGC TTCAGAGAACCTGCGGCAGGAGCTGACCTCACAGGCTGAGCGTGCGGAGGAGCTGGGCCAAGAATTGAAG GCGTGGCAGGAGAAGTTCTTCCAGAAAGAGCAGGCCCTCTCCACCCTGCAGCTCGAGCACACCAGCACAC AGGCCCTGGTGAGTGAGCTGCTGCCAGCTAAGCACCTCTGCCAGCAGCTGCAGGCCGAGCAGGCCGCTGC CGAGAAACGCCACCGTGAGGAGCTGGAGCAGAGCAAGCAGGCCGCTGGGGGACTGCGGGCAGAGCTGCTG CGGGCCCAGCGGGAGCTTGGGGAGCTGATTCCTCTGCGGCAGAAGGTGGCAGAGCAGGAGCGAACAGCTC AGCAGCTGCGGGCAGAGAAGGCCAGCTATGCAGAGCAGCTGAGCATGCTGAAGAAGGCGCATGGCCTGCT GGCAGAGGAGAACCGGGGGCTGGGTGAGCGGGCCAACCTTGGCCGGCAGTTTCTGGAAGTGGAGTTGGAC CAGGCCCGGGAGAAGTATGTCCAAGAGTTGGCAGCCGTACGTGCTGATGCTGAGACCCGTCTGGCTGAGG TGCAGCGAGAAGCACAGAGCACTGCCCGGGAGCTGGAGGTGATGACTGCCAAGTATGAGGGTGCCAAGGT CAAGGTCCTGGAGGAGAGGCAGCGGTTCCAGGAAGAGAGGCAGAAACTCACTGCCCAGGTGGAAGAACTG AGTAAGAAACTGGCTGACTCTGACCAAGCCAGCAAGGTGCAGCAGCAGAAGCTGAAGGCTGTCCAGGCTC AGGGAGGCGAGAGCCAGCAGGAGGCCCAGCGCCTCCAGGCCCAGCTGAATGAACTGCAAGCCCAGTTGAG CCAGAAGGAGCAGGCAGCTGAGCACTATAAGCTGCAGATGGAGAAAGCCAAAACACATTATGATGCCAAG AAGCAGCAGAACCAAGAGCTGCAGGAGCAGCTGCGGAGCCTGGAGCAGCTGCAGAAGGAAAACAAAGAGC TGCGAGCTGAAGCTGAACGGCTGGGCCATGAGCTACAGCAGGCTGGGCTGAAGACCAAGGAGGCTGAACA GACCTGCCGCCACCTTACTGCCCAGGTGCGCAGCCTGGAGGCACAGGTTGCCCATGCAGACCAGCAGCTT CGAGACCTGGGCAAATTCCAGGTGGCAACTGATGCTTTAAAGAGCCGTGAGCCCCAGGCTAAGCCCCAGC TGGACTTGAGTATTGACAGCCTGGATCTGAGCTGCGAGGAGGGGACCCCACTCAGTATCACCAGCAAGCT GCCTCGTACCCAGCCAGACGGCACCAGCGTCCCTGGAGAACCAGCCTCACCTATCTCCCAGCGCCTGCCC CCCAAGGTAGAATCCCTGGAGAGTCTCTACTTCACTCCCATCCCTGCTCGGAGTCAGGCCCCCCTGGAGA GCAGCCTGGACTCCCTGGGAGACGTCTTCCTGGACTCGGGTCGTAAGACCCGCTCCGCTCGTCGGCGCAC CACGCAGATCATCAACATCACCATGACCAAGAAGCTAGATGTGGAAGAGCCAGACAGCGCCAACTCATCG TTCTACAGCACGCGGTCTGCTCCTGCTTCCCAGGCTAGCCTGCGAGCCACCTCCTCTACTCAGTCTCTAG CTCGCCTGGGTTCTCCCGATTATGGCAACTCAGCCCTGCTCAGCTTGCCTGGCTACCGCCCCACCACTCG CAGTTCTGCTCGTCGTTCCCAGGCCGGGGTGTCCAGTGGGGCCCCTCCAGGAAGGAACAGCTTCTACATG GGCACTTGCCAGGATGAGCCTGAGCAGCTGGATGACTGGAACCGCATTGCAGAGCTGCAGCAGCGCAATC GAGTGTGCCCCCCACATCTGAAGACCTGCTATCCCCTGGAGTCCAGGCCTTCCCTGAGCCTGGGCACCAT CACAGATGAGGAGATGAAAACTGGAGACCCCCAAGAGACCCTGCGCCGAGCCAGCATGCAGCCAATCCAG ATAGCCGAGGGCACTGGCATCACCACCCGGCAGCAGCGCAAACGGGTCTCCCTAGAGCCCCACCAGGGCC CTGGAACTCCTGAGTCTAAGAAGGCCACCAGCTGTTTCCCACGCCCCATGACTCCCCGAGACCGACATGA AGGGCGCAAACAGAGCACTACTGAGGCCCAGAAGAAAGCAGCTCCAGCTTCTACTAAACAGGCTGACCGG CGCCAGTCGATGGCCTTCAGCATCCTCAACACACCCAAGAAGCTAGGGAACAGCCTTCTGCGGCGGGGAG CCTCAAAGAAGGCCCTGTCCAAGGCTTCCCCCAACACTCGCAGTGGAACCCGCCGTTCTCCGCGCATTGC CACCACCACAGCCAGCGCCGCCACTGCTGCCGCCATTGGTGCCACCCCTCGAGCCAAGGGCAAGGCAAAG CAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240233 representing NM_001286561 Red=Cloning site Green=Tags(s) MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQK NRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDG LNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQ TPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEE LRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSK ATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQ EAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQ AHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSL KQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEA RDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQL QEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEAR LQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKE ECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMA ATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNE LERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQE ALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGP KLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQR ELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESE KSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELK AWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELL RAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELD QAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEEL SKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAK KQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQL RDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLP PKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSS FYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYM GTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQ IAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADR RQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAK H TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286561
ORF Size	6303 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286561.1, NP_001273490.1
RefSeq Size	7431 bp
RefSeq ORF	6306 bp
Locus ID	4926
UniProt ID	Q14980
MW	237 kDa
Gene Summary	This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

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