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Collagen V (COL5A1) (NM_001278074) Human Tagged ORF Clone
CAT＃: RG240216
- TrueORF®
COL5A1 (tGFP-tagged) - Human collagen, type V, alpha 1 (COL5A1), transcript variant 2
ORF Plasmid: DDK
"NM_001278074" in other vectors (2)
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CNY 18,720.00
货期*
2周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EDSC; EDSCL1; FMDMF |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG240216 representing NM_001278074.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACGTCCATACCCGCTGGAAAGCGCGCAGCGCGCTCCGCCCGGGCGCCCCGCTGCTGCCCCCGCTG CTGCTGCTGCTGCTGTGGGCGCCGCCTCCGAGCCGCGCAGCTCAGCCAGCAGATCTCCTGAAGGTTCTA GATTTTCACAACTTGCCTGATGGAATAACAAAGACAACAGGCTTTTGCGCCACGCGGCGATCTTCCAAA GGCCCGGATGTCGCTTACAGAGTCACCAAAGACGCGCAGCTCAGCGCACCCACCAAGCAGCTGTACCCT GCGTCTGCATTTCCCGAGGACTTCTCCATCCTAACAACTGTGAAAGCCAAGAAAGGCAGCCAGGCCTTC CTGGTCTCCATCTACAACGAGCAGGGTATCCAGCAGATTGGGCTGGAGCTGGGCCGCTCTCCCGTCTTC CTCTACGAGGACCACACGGGGAAGCCTGGCCCGGAAGACTACCCCCTCTTCCGGGGCATCAACCTGTCA GATGGCAAGTGGCACAGAATTGCTCTCAGCGTCCACAAGAAAAATGTCACCTTGATCCTCGACTGTAAA AAGAAGACCACCAAATTCCTCGACCGCAGCGACCACCCCATGATCGACATCAATGGCATCATCGTGTTT GGCACCCGGATCCTGGATGAGGAGGTGTTTGAGGGTGACATCCAGCAGCTGCTCTTTGTCTCGGACCAC CGGGCAGCTTATGATTACTGTGAGCACTACAGCCCTGACTGTGACACCGCAGTACCTGACACCCCACAG TCGCAGGACCCCAATCCAGATGAATATTACACGGAAGGAGACGGCGAGGGTGAGACCTATTACTACGAA TACCCCTACTACGAAGACCCCGAAGACCTAGGGAAGGAGCCCACCCCCAGCAAGAAGCCCGTGGAAGCT GCCAAAGAAACCACAGAGGTCCCCGAGGAGCTGACCCCGACCCCCACGGAAGCTGCTCCCATGCCTGAA ACCAGTGAAGGGGCTGGGAAGGAAGAGGACGTCGGCATCGGGGACTATGACTACGTGCCCAGTGAGGAC TACTACACGCCCTCACCGTATGATGACCTCACCTATGGCGAGGGGGAGGAGAACCCCGACCAGCCCACA GACCCAGGCGCTGGGGCCGAAATTCCCACCAGCACCGCCGACACCTCCAACTCCTCCAATCCAGCTCCG CCTCCAGGGGAAGGTGCGGATGACTTGGAGGGGGAGTTCACTGAGGAAACGATCCGGAACCTTGACGAG AACTACTACGACCCCTACTACGACCCCACCAGCTCCCCGTCGGAGATCGGGCCGGGAATGCCGGCGAAC CAGGATACCATCTATGAAGGGATTGGAGGACCTCGGGGCGAGAAAGGCCAAAAGGGAGAACCAGCGATT ATCGAGCCGGGCATGCTCATCGAGGGCCCGCCTGGCCCAGAAGGCCCCGCGGGTCTTCCCGGACCTCCA GGAACCATGGGTCCCACTGGCCAAGTCGGGGACCCTGGAGAAAGGGGCCCCCCTGGACGCCCAGGCCTT CCTGGGGCCGATGGCCTGCCCGGTCCTCCAGGAACCATGCTCATGCTGCCCTTCCGGTTTGGAGGTGGC GGCGATGCGGGCTCCAAAGGCCCCATGGTCTCAGCCCAGGAGTCCCAGGCGCAAGCCATTCTCCAGCAG GCCAGGTTGGCACTGAGGGGACCAGCTGGCCCGATGGGTCTCACAGGGAGACCTGGCCCTGTGGGTCCC CCTGGGAGCGGAGGTTTGAAGGGCGAGCCGGGAGACGTGGGGCCTCAGGGTCCTCGAGGTGTGCAAGGC CCGCCTGGTCCGGCCGGGAAGCCCGGAAGACGGGGTCGGGCTGGGAGTGATGGAGCCAGAGGAATGCCT GGACAAACTGGCCCCAAGGGTGACCGGGGTTTCGACGGCCTGGCTGGGTTGCCAGGCGAGAAGGGCCAC AGGGGTGACCCTGGTCCTTCCGGCCCACCAGGACCTCCGGGAGACGATGGAGAAAGGGGTGACGACGGA GAAGTTGGGCCCAGGGGGCTGCCTGGGGAGCCCGGGCCACGTGGTCTGCTTGGGCCGAAGGGGCCCCCA GGTCCTCCCGGACCTCCCGGTGTCACGGGTATGGACGGCCAGCCGGGGCCAAAAGGAAATGTGGGTCCC CAGGGAGAGCCTGGCCCCCCAGGACAGCAGGGTAATCCAGGCGCCCAGGGTCTTCCAGGCCCCCAGGGT GCAATTGGTCCTCCAGGAGAAAAGGGTCCCTTGGGGAAACCAGGCCTTCCAGGAATGCCCGGTGCTGAC GGACCCCCGGGACACCCTGGCAAAGAAGGCCCTCCAGGAGAGAAAGGAGGTCAGGGTCCACCTGGCCCC CAGGGTCCGATTGGCTACCCAGGTCCTCGAGGAGTCAAGGGGGCCGATGGCATCCGTGGTCTGAAGGGC ACAAAGGGCGAGAAGGGTGAAGACGGCTTTCCTGGGTTTAAAGGAGACATGGGCATCAAGGGTGATCGG GGGGAGATCGGCCCACCCGGTCCCAGGGGAGAAGATGGCCCTGAAGGCCCAAAGGGTCGCGGAGGTCCC AATGGTGACCCCGGTCCTCTGGGACCCCCTGGGGAGAAGGGAAAACTCGGAGTCCCAGGGTTACCAGGG TATCCAGGAAGACAAGGACCAAAGGGCTCTATTGGATTCCCTGGATTTCCTGGCGCCAATGGAGAGAAG GGCGGCAGGGGGACCCCTGGAAAGCCAGGACCGCGGGGGCAGCGAGGCCCAACGGGTCCGAGGGGTGAA AGAGGCCCCCGGGGCATCACTGGGAAGCCTGGCCCCAAGGGCAACTCCGGAGGTGACGGCCCAGCTGGC CCTCCTGGTGAACGGGGACCCAATGGACCCCAAGGACCCACAGGATTTCCTGGACCAAAGGGCCCCCCT GGCCCTCCAGGCAAGGATGGACTCCCAGGACACCCTGGACAGAGAGGCGAGACTGGTTTCCAAGGCAAG ACCGGCCCTCCAGGCCCCCCCGGCGTGGTCGGCCCTCAGGGTCCCACGGGAGAAACGGGCCCAATGGGT GAGCGTGGCCACCCTGGGCCCCCTGGACCCCCCGGTGAACAGGGGCTTCCGGGCCTTGCTGGAAAAGAA GGGACGAAGGGTGACCCAGGCCCTGCAGGCCTCCCTGGGAAAGATGGCCCTCCAGGATTACGTGGTTTC CCTGGGGACCGAGGGCTTCCTGGTCCAGTGGGAGCTCTTGGACTGAAAGGCAATGAAGGGCCCCCTGGC CCACCAGGCCCTGCGGGATCTCCAGGGGAGAGAGGTCCAGCTGGAGCCGCTGGGCCCATCGGAATTCCA GGGAGACCTGGGCCCCAGGGACCCCCAGGGCCGGCAGGAGAGAAAGGGGCTCCTGGCGAGAAAGGCCCA CAAGGCCCAGCTGGCCGAGACGGTCTCCAGGGGCCTGTGGGGCTCCCGGGTCCAGCTGGCCCTGTGGGT CCCCCTGGAGAAGACGGAGATAAGGGAGAGATCGGGGAGCCGGGGCAGAAAGGAAGCAAGGGGGACAAA GGAGAACAGGGTCCTCCTGGGCCTACAGGTCCTCAAGGCCCCATCGGACAGCCAGGCCCCTCTGGAGCT GACGGCGAGCCGGGGCCTCGGGGCCAGCAGGGCCTTTTCGGGCAGAAAGGTGATGAAGGTCCCAGAGGC TTTCCTGGACCCCCTGGGCCAGTGGGGCTGCAGGGTTTGCCAGGACCTCCAGGCGAGAAGGGTGAGACA GGAGACGTGGGCCAGATGGGCCCCCCGGGTCCCCCTGGCCCCCGAGGACCCTCCGGAGCTCCAGGTGCT GATGGCCCACAAGGTCCCCCAGGTGGAATAGGAAACCCTGGTGCAGTGGGAGAGAAGGGCGAGCCTGGC GAAGCAGGTGAGCCTGGCCTTCCGGGAGAAGGCGGCCCCCCGGGACCCAAAGGAGAAAGGGGAGAGAAG GGCGAGTCAGGCCCTTCAGGTGCTGCCGGACCCCCTGGACCCAAAGGCCCTCCCGGAGATGATGGTCCC AAAGGCAGCCCTGGCCCAGTGGGTTTTCCTGGAGATCCTGGCCCCCCCGGAGAGCCTGGCCCCGCGGGT CAAGATGGTCCCCCTGGTGACAAAGGAGATGATGGTGAACCCGGGCAGACGGGATCCCCCGGCCCTACT GGTGAACCAGGTCCATCGGGGCCTCCAGGAAAAAGGGGTCCCCCAGGCCCCGCAGGCCCCGAAGGCAGA CAGGGAGAGAAAGGGGCCAAGGGAGAAGCCGGCTTGGAAGGCCCTCCTGGGAAGACTGGCCCCATCGGC CCCCAGGGGGCCCCTGGGAAGCCCGGACCGGATGGCCTTCGAGGGATCCCTGGCCCTGTGGGAGAACAA GGTCTCCCAGGATCCCCAGGCCCGGACGGTCCCCCCGGCCCCATGGGTCCCCCAGGACTTCCCGGCCTC AAAGGAGATTCTGGTCCCAAAGGTGAAAAGGGTCATCCAGGCCTGATCGGGCTCATCGGTCCTCCGGGT GAACAGGGTGAGAAGGGCGACCGTGGTCTCCCTGGCCCCCAGGGCTCCTCCGGTCCTAAGGGAGAACAG GGTATCACTGGTCCTTCTGGCCCGATTGGGCCTCCTGGGCCCCCTGGCCTGCCGGGTCCGCCTGGTCCA AAAGGTGCTAAGGGCTCCTCGGGTCCAACTGGCCCGAAGGGTGAGGCAGGCCACCCAGGACCCCCAGGC CCCCCGGGCCCCCCGGGAGAGGTCATCCAGCCCCTGCCAATCCAGGCATCCAGGACGCGGCGGAACATC GACGCCAGCCAGCTGCTGGACGACGGGAATGGCGAGAACTACGTGGACTACGCGGACGGCATGGAAGAG ATCTTCGGCTCTCTCAACTCTCTGAAGCTGGAGATTGAGCAGATGAAACGGCCCCTGGGCACGCAGCAG AACCCCGCCCGCACCTGCAAGGACCTGCAGCTCTGCCACCCCGACTTCCCAGATGGTGAATACTGGGTC GATCCTAACCAAGGATGCTCCAGGGATTCCTTCAAGGTTTACTGCAACTTCACAGCCGGGGGGTCGACA TGCGTCTTCCCTGACAAGAAGTCCGAAGGGAGTAAAATGGCCCGCTGGCCCAAAGAGCAGCCTTCCACC TGGTATAGTCAGTACAAGCGGGGGTCCCTGCTCTCCTATGTGGACGCCGAGGGCAACCCTGTGGGTGTG GTACAGATGACCTTCCTGCGGCTGCTGAGCGCCTCTGCCCACCAGAACGTCACCTACCACTGCTACCAG TCAGTGGCCTGGCAGGACGCAGCCACGGGCAGCTACGACAAGGCCCTCCGCTTCCTGGGCTCCAACGAC GAGGAGATGTCCTATGACAACAACCCCTACATCCGCGCCCTGGTGGACGGCTGTGCTACCAAGAAAGGC TACCAGAAGACGGTTCTGGAGATCGACACCCCCAAAGTGGAGCAGGTGCCCATCGTGGACATCATGTTC AATGACTTCGGTGAAGCGTCACAGAAATTTGGATTTGAAGTGGGGCCGGCTTGCTTCATGGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG240216
Blue=ORF Red=Cloning site Green=Tag(s) MDVHTRWKARSALRPGAPLLPPLLLLLLWAPPPSRAAQPADLLKVLDFHNLPDGITKTTGFCATRRSSK GPDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQIGLELGRSPVF LYEDHTGKPGPEDYPLFRGINLSDGKWHRIALSVHKKNVTLILDCKKKTTKFLDRSDHPMIDINGIIVF GTRILDEEVFEGDIQQLLFVSDHRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYTEGDGEGETYYYE YPYYEDPEDLGKEPTPSKKPVEAAKETTEVPEELTPTPTEAAPMPETSEGAGKEEDVGIGDYDYVPSED YYTPSPYDDLTYGEGEENPDQPTDPGAGAEIPTSTADTSNSSNPAPPPGEGADDLEGEFTEETIRNLDE NYYDPYYDPTSSPSEIGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPP GTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQ ARLALRGPAGPMGLTGRPGPVGPPGSGGLKGEPGDVGPQGPRGVQGPPGPAGKPGRRGRAGSDGARGMP GQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGPPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPP GPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGAD GPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDR GEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEK GGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPP GPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGLAGKE GTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGNEGPPGPPGPAGSPGERGPAGAAGPIGIP GRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDK GEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGET GDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEK GESGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPT GEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQ GLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGEQ GITGPSGPIGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHPGPPGPPGPPGEVIQPLPIQASRTRRNI DASQLLDDGNGENYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWV DPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGSKMARWPKEQPSTWYSQYKRGSLLSYVDAEGNPVGV VQMTFLRLLSASAHQNVTYHCYQSVAWQDAATGSYDKALRFLGSNDEEMSYDNNPYIRALVDGCATKKG YQKTVLEIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFMG TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001278074 |
| ORF Size | 5514 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001278074.1, NP_001265003.1 |
| RefSeq Size | 8455 bp |
| RefSeq ORF | 5517 bp |
| Locus ID | 1289 |
| Protein Families | Druggable Genome |
| Protein Pathways | ECM-receptor interaction, Focal adhesion |
| MW | 184.1 kDa |
| Gene Summary | This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] |
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