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RPB2 (POLR2B) (NM_001303269) Human Tagged ORF Clone
CAT＃: RG240015
- TrueORF®
POLR2B (tGFP-tagged) - Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B), transcript variant 3
ORF Plasmid: DDK
"NM_001303269" in other vectors (2)
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CNY 12,070.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | hRPB140; POL2RB; RPB2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG240015 representing NM_001303269.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAATATGATGAGGATGATGATGAAATCACCCCGGATTTGTGGCAAGAAGCATGCTGGATTGTAATC AGTTCCTATTTTGACGAGAAAGGCTTGGTTAGACAACAGCTGGATTCTTTTGATGAGTTTATTCAGATG TCTGTTCAAAGAATTGTGGAAGACGCTCCTCCTATAGACCTACAGGCTGAAGCTCAGCATGCTAGTGGA GAAGTTGAAGAACCGCCACGATATTTGCTGAAGTTTGAACAAATTTATCTTTCCAAGCCTACCCATTGG GAAAGAGATGGTGCTCCTTCACCAATGATGCCCAATGAAGCTAGATTAAGGAATCTCACGTATTCTGCT CCGCTTTATGTTGATATAACAAAAACAGTCATTAAAGAAGGTGAAGAACAACTTCAGACTCAGCATCAG AAAACTTTTATAGGAAAAATTCCAATTATGTTGCGGTCAACTTACTGCCTTTTGAATGGCTTGACAGAT CGTGATCTTTGTGAGTTAAATGAATGCCCTTTGGATCCTGGTGGCTATTTCATTATTAATGGATCAGAA AAGGTTCTGATTGCCCAAGAGAAAATGGCAACAAACACAGTTTATGTGTTTGCCAAAAAGGATTCTAAA TATGCCTACACAGGAGAGTGTAGATCATGTCTTGAGAATTCTTCCCGACCCACCAGTACTATATGGGTT AGCATGCTGGCAAGAGGAGGACAGGGTGCCAAGAAGAGTGCTATTGGTCAGCGCATTGTGGCAACTCTA CCATATATCAAGCAAGAAGTTCCCATCATTATTGTGTTCAGAGCATTAGGTTTTGTGTCCGACAGAGAT ATTTTAGAACATATTATTTATGATTTTGAAGATCCAGAGATGATGGAAATGGTTAAACCTTCTCTCGAT GAAGCTTTTGTCATCCAAGAACAGAATGTTGCACTAAATTTCATTGGTTCAAGAGGAGCAAAGCCTGGT GTTACTAAAGAGAAAAGAATTAAATATGCAAAGGAAGTTTTACAAAAAGAAATGCTCCCTCATGTTGGT GTCAGTGATTTTTGTGAGACCAAAAAAGCCTATTTCTTGGGATACATGGTTCATAGGTTACTTCTGGCA GCTTTGGGTAGAAGAGAACTAGATGACAGAGATCACTATGGAAACAAGAGATTGGATCTTGCTGGGCCG CTGCTTGCATTCTTATTTAGAGGTATGTTTAAGAATTTGCTTAAAGAAGTGCGGATCTATGCACAGAAA TTTATTGATCGAGGAAAGGATTTTAACTTGGAGTTGGCAATTAAAACACGGATCATATCTGATGGCCTA AAATACTCTTTAGCTACTGGAAACTGGGGTGATCAAAAGAAAGCTCATCAAGCCAGAGCTGGAGTATCT CAGGTGTTAAACCGCCTGACTTTTGCGTCTACTCTTTCTCACCTGCGTCGTTTAAATTCTCCTATTGGT AGAGACGGCAAGCTAGCAAAACCAAGACAGTTGCATAATACGTTGTGGGGAATGGTGTGTCCTGCCGAG ACCCCAGAGGGCCATGCTGTAGGACTTGTGAAGAATTTAGCCTTGATGGCGTATATTTCAGTTGGATCT CAACCATCTCCAATTCTGGAATTTTTAGAAGAATGGAGTATGGAAAATTTAGAAGAAATTTCTCCTGCA GCTATTGCTGATGCAACCAAGATTTTTGTTAATGGCTGCTGGGTTGGAATACATAAAGATCCCGAACAA CTTATGAACACCCTAAGGAAATTGAGACGTCAGATGGACATCATTGTGTCTGAAGTTTCTATGATCAGA GATATTCGAGAGAGGGAGATTCGGATCTATACGGATGCAGGCCGTATTTGTAGACCACTTCTGATTGTG GAAAAACAAAAGCTACTTTTGAAGAAGAGGCATATTGACCAATTGAAAGAGAGAGAATATAACAACTAT AGTTGGCAGGATCTTGTGGCCAGTGGGGTAGTGGAGTATATTGATACCCTGGAAGAAGAAACAGTGATG CTTGCAATGACTCCAGATGATTTACAGGAGAAAGAAGTAGCTTATTGTTCCACATATACACACTGTGAG ATTCATCCCTCAATGATCCTTGGTGTCTGTGCATCTATTATTCCCTTTCCTGATCATAACCAGTCCCCT AGAAACACATACCAGTCTGCTATGGGTAAGCAGGCTATGGGAGTTTACATCACCAACTTCCATGTTCGC ATGGACACATTGGCCCATGTTCTCTATTATCCTCAAAAGCCACTTGTGACTACACGGTCTATGGAATAT CTACGATTTAGAGAGCTGCCAGCAGGCATCAACTCAATTGTGGCCATTGCATCATACACTGGATATAAT CAGGAAGACTCTGTTATCATGAATCGTTCAGCTGTAGACCGCGGCTTCTTCAGGTCTGTTTTCTATCGC TCATACAAAGAACAGGAGTCTAAAAAAGGATTTGATCAAGAAGAAGTTTTTGAGAAGCCTACACGTGAA ACATGCCAGGGCATGAGGCATGCCATTTACGACAAGCTGGATGATGATGGTTTGATAGCTCCAGGGGTT CGTGTATCAGGAGATGATGTTATTATAGGCAAAACAGTCACCTTGCCTGAAAATGAAGATGAATTGGAG AGCACCAATAGACGCTATACCAAGAGAGACTGTAGCACTTTTCTCAGAACTAGCGAGACGGGCATTGTG GATCAGGTTATGGTAACTCTCAATCAGGAAGGATATAAATTTTGTAAAATAAGGGTACGCTCTGTTAGG ATTCCACAGATTGGAGACAAATTTGCTAGTCGACATGGTCAAAAGGGTACTTGTGGTATTCAGTATAGA CAAGAGGATATGCCTTTCACCTGTGAAGGTATCACCCCTGATATCATCATCAATCCCCATGCCATCCCC TCTCGTATGACTATTGGTCACTTAATTGAATGCCTTCAAGGGAAGGTATCGGCTAACAAGGGTGAAATT GGTGATGCCACTCCATTTAATGATGCTGTTAACGTGCAGAAGATTTCTAATCTTTTATCTGATTATGGC TATCATCTCAGAGGAAATGAGGTCCTGTACAATGGGTTCACTGGTCGAAAAATCACATCACAAATATTT ATTGGCCCCACTTATTACCAGCGTTTGAAGCATATGGTGGATGATAAGATTCACTCTCGTGCTAGGGGA CCTATTCAGATCCTCAATAGACAGCCCATGGAGGGTAGATCTCGTGATGGTGGCCTGCGTTTTGGAGAA ATGGAACGAGATTGTCAGATTGCCCATGGAGCAGCCCAGTTTTTAAGGGAAAGATTGTTTGAGGCATCA GATCCATATCAGGTTCATGTTTGCAATCTTTGTGGAATAATGGCGATTGCCAACACCAGGACCCATACA TATGAATGCAGGGGCTGCCGCAATAAAACCCAGATTTCTTTGGTGCGAATGCCTTACGCATGCAAACTA TTGTTTCAGGAACTTATGTCTATGAGTATTGCACCGCGAATGATGAGTGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG240015
Blue=ORF Red=Cloning site Green=Tag(s) MQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASG EVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQ KTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSK YAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRD ILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVG VSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQK FIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIG RDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPA AIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIV EKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCE IHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEY LRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRE TCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIV DQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIP SRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIF IGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEAS DPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001303269 |
| ORF Size | 3501 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001303269.1, NP_001290198.1 |
| RefSeq Size | 4240 bp |
| RefSeq ORF | 3504 bp |
| Locus ID | 5431 |
| UniProt ID | P30876 |
| Protein Families | Transcription Factors |
| Protein Pathways | Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
| MW | 133.5 kDa |
| Gene Summary | This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC240015 | POLR2B (myc-DDK-tagged) - Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B), transcript variant 3 |
CNY 11,690.00 |
|
| SC337909 | POLR2B (untagged) - Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B), transcript variant 3 |
CNY 11,210.00 |
