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PHF19 (NM_001286842) Human Tagged ORF Clone
CAT＃: RG237787
- TrueORF®
PHF19 (tGFP-tagged) - Human PHD finger protein 19 (PHF19), transcript variant 4
ORF Plasmid: DDK
"NM_001286842" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MTF2L1; PCL3; TDRD19B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG237787 representing NM_001286842.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGCAATGTTACCGGTGCAGGCAGTGGTTCCACGAGGCCTGCACCCAGTGCCTCAATGAGCCCATG ATGTTTGGAGACCGGTTTTACCTGTTCTTCTGCTCCGTGTGTAACCAGGGCCCAGAGTACATCGAGAGG CTGCCCCTGCGATGGGTGGATGTGGTTCACCTGGCCCTCTATAATCTGGGGGTACAGAGCAAGAAGAAG TACTTTGACTTTGAGGAGATTCTGGCCTTTGTCAACCACCACTGGGAGCTCCTGCAGCTTGGCAAGCTC ACCAGCACCCCAGTGACAGATCGAGGACCACATCTCCTCAACGCTCTGAACAGTTATAAAAGCCGGTTC CTCTGCGGCAAGGAGATCAAGAAGAAGAAGTGCATCTTCCGCCTGCGCATCCGCGTCCCACCCAACCCG CCAGGGAAGCTGCTGCCTGACAAAGGACTGCTGCCAAATGAGAACAGCGCCTCCTCTGAGCTGCGTAAG AGAGGAAAGAGCAAGCCTGGTTTGTTGCCTCACGAATTCCAGCAGCAGAAAAGGCGAGTTTATAGAAGA AAAAGATCAAAGTTTTTGCTGGAAGATGCTATTCCCAGTAGTGACTTCACCTCAGCCTGGAGCACCAAC CACCACCTGGCTAGCATATTTGACTTCACGCTGGATGAAATTCAAAGTTTAAAAAGTGCCAGCTCAGGC CAGACCTTCTTCTCAGATGTCGACTCCACCGACGCTGCCAGCACCTCTGGCTCTGCCTCCACCAGCCTC TCCTATGACTCCAGATGGACAGTGGGCAGCCGAAAGAGGAAGCTGGCAGCCAAGGCATACATGCCCCTG CGGGCAAAGCGGTGGGCAGCTGAGCTGGATGGACGCTGCCCCTCGGACAGCAGTGCAGAGGGGGCTTCA GTCCCCGAGCGGCCAGACGAAGGCATTGACAGCCACACATTTGAGAGCATCAGTGAAGATGACTCATCC CTGTCCCACCTCAAGTCATCTATCACCAACTACTTTGGTGCAGCTGGGCGGTTGGCCTGTGGGGAGAAG TACCAGGTGTTGGCTCGGAGGGTCACACCTGAGGGCAAGGTTCAGTACCTGGTGGAGTGGGAAGGGACC ACCCCTTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG237787
Blue=ORF Red=Cloning site Green=Tag(s) MLQCYRCRQWFHEACTQCLNEPMMFGDRFYLFFCSVCNQGPEYIERLPLRWVDVVHLALYNLGVQSKKK YFDFEEILAFVNHHWELLQLGKLTSTPVTDRGPHLLNALNSYKSRFLCGKEIKKKKCIFRLRIRVPPNP PGKLLPDKGLLPNENSASSELRKRGKSKPGLLPHEFQQQKRRVYRRKRSKFLLEDAIPSSDFTSAWSTN HHLASIFDFTLDEIQSLKSASSGQTFFSDVDSTDAASTSGSASTSLSYDSRWTVGSRKRKLAAKAYMPL RAKRWAAELDGRCPSDSSAEGASVPERPDEGIDSHTFESISEDDSSLSHLKSSITNYFGAAGRLACGEK YQVLARRVTPEGKVQYLVEWEGTTPY TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001286842 |
| ORF Size | 1113 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001286842.1, NP_001273771.1 |
| RefSeq Size | 3547 bp |
| RefSeq ORF | 1116 bp |
| Locus ID | 26147 |
| Protein Families | Druggable Genome |
| MW | 42.6 kDa |
| Gene Summary | Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds dimethylated at 'Lys-36' (H3K36me2). Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter.[UniProtKB/Swiss-Prot Function] |
Documents
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