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MDFI (NM_001300806) Human Tagged ORF Clone
CAT＃: RG236901
- TrueORF®
MDFI (tGFP-tagged) - Human MyoD family inhibitor (MDFI), transcript variant 4
ORF Plasmid: DDK
"NM_001300806" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | I-MF; I-mfa |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG236901 representing NM_001300806.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTACCAGGTGAGCGGCCAGCGCCCCTCTGGCTGCGACGCGCCCTATGGAGCCCCCAGCGCAGCCCCG GGCCCAGCCCAGACCCTATCCCTCCTTCCTGGGCTGGAGGTAGTAACAGGATCCACTCACCCTGCGGAG GCAGCACCAGAGGAGGGCTCCCTGGAGGAGGCGGCAACCCCCATGCCCCAAGGCAATGGCCCTGGCATC CCCCAGGGCCTGGACAGCACTGACCTCGACGTCCCCACAGAAGCTGTGACATGCCAGCCTCAGGGGAAC CCCTTGGGCTGCACCCCACTTCTGCCGAATGACTCTGGCCACCCCTCAGAGCTGGGCGGCACCAGACGG GCGGGGAATGGTGCCCTGGGTGGCCCCAAGGCCCACCGGAAGTTGCAGACACACCCATCTCTCGCCAGC CAGGGCAGCAAGAAGAGTAAGAGCAGCAGCAAATCCACCACCTCCCAGATCCCCCTCCAGGCACAGGAA GACTGCTGTGTCCACTGCATCCTGTCCTGCCTGTTCTGCGAGTTCCTGACGCTGTGCAACATCGTCCTG GACTGCGCCACCTGTGGCTCCTGCAGCTCGGAGGACTCGTGCCTCTGCTGCTGCTGCTGTGGCTCTGGC GAGTGTGCCGACTGCGACCTGCCCTGCGACCTGGACTGCGGCATCCTGGATGCCTGCTGCGAGTCCGCG GACTGCCTGGAGATCTGCATGGAGTGCTGTGGGCTCTGCTTCTCCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG236901
Blue=ORF Red=Cloning site Green=Tag(s) MYQVSGQRPSGCDAPYGAPSAAPGPAQTLSLLPGLEVVTGSTHPAEAAPEEGSLEEAATPMPQGNGPGI PQGLDSTDLDVPTEAVTCQPQGNPLGCTPLLPNDSGHPSELGGTRRAGNGALGGPKAHRKLQTHPSLAS QGSKKSKSSSKSTTSQIPLQAQEDCCVHCILSCLFCEFLTLCNIVLDCATCGSCSSEDSCLCCCCCGSG ECADCDLPCDLDCGILDACCESADCLEICMECCGLCFSS TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001300806 |
| ORF Size | 738 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001300806.2 |
| RefSeq Size | 1720 bp |
| RefSeq ORF | 741 bp |
| Locus ID | 4188 |
| UniProt ID | Q99750 |
| Protein Families | Transcription Factors |
| MW | 25.5 kDa |
| Gene Summary | This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq, Jul 2008] |
Documents
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