ZSWIM8 (NM_001242487) Human Tagged ORF Clone
CAT#: RG235338
- TrueORF®
ZSWIM8 (tGFP-tagged) - Homo sapiens zinc finger, SWIM-type containing 8 (ZSWIM8), transcript variant 2
ORF Plasmid: DDK
"NM_001242487" in other vectors (2)
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CNY 20,140.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | KIAA0913 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG235338 representing NM_001242487
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGATGTTTGCAGAGTGGGAGGACGGAGAGCGCTTCTCATTCGAGGATTCGGACCGTTTTGAGG AGGATTCACTCTGTTCCTTCATCTCCGAGGCCGAGAGCCTCTGCCAGAACTGGCGGGGATGGCGCAAACA GTCAGCGGGGCCCAATTCCCCCACTGGCGGCGGTGGCGGAGGTGGCAGTGGCGGTACCAGAATGCGAGAT GGACTGGTGATCCCATTGGTGGAGCTGTCAGCAAAGCAGGTGGCATTTCATATCCCATTTGAAGTGGTGG AGAAAGTTTACCCACCAGTGCCTGAGCAGCTACAGCTCCGAATTGCTTTTTGGAGCTTCCCTGAGAATGA AGAGGACATTCGGCTGTATTCGTGCCTGGCCAATGGCAGTGCGGATGAGTTTCAGCGAGGGGATCAGCTC TTCCGCATGCGGGCTGTGAAGGACCCATTGCAGATAGGGTTCCACCTGAGTGCTACAGTGGTGCCACCTC AGATGGTCCCTCCTAAAGGGGCCTACAACGTGGCTGTGATGTTTGACCGCTGCCGGGTCACTTCCTGCAG CTGTACCTGTGGGGCTGGGGCCAAATGGTGCACCCACGTCGTGGCACTCTGTCTCTTCCGCATCCACAAC GCTTCTGCAGTCTGCCTGCGAGCCCCAGTCTCAGAGTCCCTGTCCCGGCTACAGAGGGACCAGCTGCAAA AGTTTGCTCAGTACCTCATCAGTGAGCTCCCTCAGCAGATCCTCCCCACAGCTCAGCGTCTCCTGGACGA ACTCCTGTCTTCCCAGTCAACAGCCATCAATACAGTGTGTGGAGCTCCGGACCCCACAGCAGGGCCCTCA GCATCGGACCAGAGTACTTGGTATCTGGATGAATCGACACTCACTGACAACATCAAAAAGACACTGCACA AGTTCTGTGGCCCCTCCCCTGTGGTCTTCAGTGATGTGAACTCCATGTATCTGTCTTCCACGGAGCCGCC AGCCGCTGCTGAATGGGCATGTCTGCTGCGCCCTCTGAGGGGCCGTGAGCCAGAGGGCGTCTGGAACCTG CTAAGCATTGTGCGGGAGATGTTCAAGCGGAGGGACAGCAATGCTGCCCCCTTGTTGGAAATCCTCACTG ACCAGTGCCTCACCTATGAACAGATAACAGGTTGGTGGTATAGCGTACGTACCTCAGCCTCACACAGCAG TGCCAGTGGGCACACGGGCCGTAGCAACGGGCAGTCAGAGGTGGCAGCCCATGCCTGTGCCAGCATGTGT GACGAGATGGTCACACTGTGGAGGCTGGCCGTGCTGGACCCTGCACTCAGCCCCCAGCGGCGCCGGGAAC TGTGTACGCAGCTGCGGCAGTGGCAACTGAAGGTGATTGAGAACGTCAAGCGGGGCCAACACAAGAAGAC GCTGGAGCGGCTCTTCCCCGGCTTCCGGCCAGCGGTGGAGGCCTGCTACTTCAACTGGGAAGAGGCCTAC CCACTTCCTGGTGTCACCTACAGCGGCACTGACAGGAAGCTGGCACTGTGCTGGGCCCGGGCCCTGCCCT CTCGGCCAGGTGCCTCCCGCTCTGGGGGCCTGGAGGAATCCCGGGACCGGCCCCGACCCCTTCCTACTGA GCCAGCTGTGCGGCCCAAGGAGCCTGGGACCAAGCGAAAGGGCTTGGGTGAGGGGGTCCCCTCATCACAG CGGGGTCCCCGCCGCCTCTCAGCTGAAGGGGGAGATAAAGCTCTACATAAGATGGGTCCAGGTGGGGGCA AAGCCAAGGCACTGGGTGGGGCTGGCAGTGGGAGCAAGGGCTCAGCAGGTGGCGGAAGCAAGCGACGGCT GAGCAGCGAAGACAGCTCCCTGGAGCCAGACCTGGCCGAGATGAGCCTGGATGACAGCAGCCTGGCCCTG GGCGCAGAGGCCAGCACCTTCGGGGGATTCCCTGAGAGCCCTCCACCCTGTCCTCTCCACGGTGGCTCCC GAGGCCCTTCCACTTTCCTTCCTGAGCCCCCAGATACTTATGAAGAAGATGGTGGTGTGTACTTCTCGGA AGGGCCTGAGCCTCCCACAGCCTCTGTTGGCCCCCCTGGCCTACTGCCTGGGGATGTCTGTACCCAGGAC GACCTCCCTTCTACAGATGAGAGTGGCAATGGGCTTCCCAAAACCAAAGAGGCAGCCCCTGCAGTTGGAG AGGAGGATGATGACTACCAGGCGTACTATCTGAATGCCCAGGATGGGGCTGGGGGCGAGGAAGAGAAGGC CGAGGGCGGGGCTGGGGAGGAGCACGACCTGTTTGCTGGGCTGAAGCCACTGGAACAGGAGAGTCGCATG GAGGTACTGTTTGCCTGTGCTGAGGCCCTGCATGCGCATGGCTATAGCAGTGAGGCCTCCCGTCTCACTG TGGAGCTTGCCCAGGATCTGCTAGCCAACCCACCCGACCTCAAGGTAGAGCCGCCCCCTGCCAAGGGCAA GAAGAACAAGGTATCCACGAGCCGTCAGACCTGGGTGGCTACCAACACCCTGAGCAAGGCGGCCTTCCTG TTGACAGTGCTAAGTGAGCGTCCAGAGCACCACAACCTGGCCTTCCGAGTTGGCATGTTTGCCTTGGAGC TACAGAGGCCTCCAGCTTCTACCAAGGCCTTGGAGGTGAAGCTGGCATACCAGGAGTCTGAGGTGGCTGC CCTGCTCAAGAAGATCCCTCTGGGTCCAAGTGAGATGAGTACCATGCGGTGCCGGGCAGAGGAACTTCGG GAGGGGACACTCTGTGACTATCGGCCTGTGTTGCCTCTCATGCTGGCCAGTTTCATCTTTGACGTTCTCT GTGCTCCAGGTTCCCGGCCCCCAAGTCGCAACTGGAACAGCGAGACACCTGGGGATGAGGAGCTTGGATT TGAAGCAGCAGTTGCTGCCTTGGGCATGAAGACAACAGTGAGCGAGGCAGAACATCCCCTCTTATGTGAA GGCACACGTCGGGAGAAGGGTGACCTGGCATTAGCACTAATGATCACTTACAAGGACGACCAGGCCAAGC TTAAGAAGATCTTAGACAAACTCTTGGACCGAGAGAGCCAGACACATAAGCCACAGACGCTGAGTTCTTT CTACTCATCTAGCCGCCCAACCACAGCCAGCCAGAGGTCTCCTTCAAAGCACGGGGGCCCATCTGCCCCA GGGGCCCTGCAACCACTGACCTCAGGCTCTGCAGGGCCTGCTCAACCAGGGAGTGTGGCAGGGGCTGGGC CAGGCCCCACTGAGGGCTTCACAGAGAAGAATGTGCCTGAGAGTTCCCCACATTCCCCCTGTGAGGGTCT TCCATCTGAGGCAGCTTTGACCCCAAGGCCAGAAGGGAAGGTTCCTAGCCGCTTGGCACTTGGCAGTCGT GGAGGCTATAATGGACGGGGATGGGGGTCCCCAGGACGGCCTAAGAAGAAGCACACAGGCATGGCCAGCA TTGACAGCAGTGCCCCTGAAACAACATCGGATAGTTCCCCCACCTTAAGCCGGAGACCACTTCGAGGGGG CTGGGCCCCCACCTCCTGGGGTCGAGGTCAGGACAGTGACAGCATTAGCAGCTCTTCTTCGGACTCCCTG GGCTCCTCATCCTCCAGTGGAAGTCGCCGGGCCAGTGCCAGTGGAGGAGCCCGGGCGAAGACTGTTGAAG TTGGCAGGTACAAGGGCCGCCGCCCCGAGAGTCATGCCCCTCATGTACCCAATCAGCCATCAGAGGCAGC TGCACACTTCTACTTCGAGCTGGCGAAGACAGTGCTGATCAAGGCAGGGGGCAACAGCAGCACTTCCATT TTCACACATCCATCTTCCTCAGGGGGCCACCAGGGTCCTCACCGCAACCTGCACCTTTGCGCCTTCGAGA TTGGGCTTTATGCCCTTGGCCTGCACAACTTTGTTTCTCCCAACTGGCTCTCACGTACTTATTCTTCCCA CGTTTCCTGGATTACAGGCCAGGCCATGGAGATAGGCAGCGCAGCCCTGACTATACTGGTAGAATGCTGG GATGGGCACCTGACACCCCCTGAGGTTGCATCCCTGGCTGACAGGGCATCACGGGCAAGAGACTCCAATA TGGTGAGGGCGGCAGCAGAGCTGGCCCTGAGCTGCCTGCCTCACGCCCATGCATTGAACCCTAATGAGAT CCAGCGGGCCCTGGTGCAGTGCAAGGAACAGGACAACCTGATGTTGGAGAAGGCCTGCATGGCAGTGGAA GAGGCAGCTAAGGGTGGGGGCGTGTACCCTGAAGTGTTGTTTGAGGTTGCTCACCAGTGGTTCTGGCTGT ATGAGCAAACTGCAGGTGGCTCATCCACAGCCCGTGAAGGGGCTACAAGCTGTAGTGCCAGTGGGATCAG GGCAGGTGGGGAAGCTGGGCGGGGTATGCCTGAGGGTAGAGGGGGCCCAGGGACTGAGCCGGTTACAGTG GCAGCGGCAGCAGTGACAGCAGCAGCCACAGTGGTGCCCGTCATATCGGTGGGGTCTAGTTTATACCCGG GTCCAGGACTGGGGCATGGCCACTCCCCTGGCCTGCACCCCTACACTGCTCTACAGCCCCACCTGCCCTG TAGCCCTCAGTATCTCACTCACCCAGCTCACCCTGCCCACCCCATGCCTCACATGCCCCGGCCTGCCGTC TTCCCTGTGCCCAGCTCTGCATACCCACAGGGTGTGCATCCTGCATTCCTAGGGGCTCAGTACCCTTATT CAGTGACTCCTCCCTCACTTGCTGCCACTGCTGTGTCTTTCCCCGTTCCTTCCATGGCACCCATCACAGT ACATCCCTACCACACAGAGCCAGGGCTTCCACTGCCCACCAGTGTGGCCTGTGAGTTGTGGGGCCAGGGA ACAGTGAGCAGTGTCCATCCAGCATCCACGTTTCCAGCCATCCAAGGTGCCTCACTGCCTGCCCTGACCA CACAGCCCAGCCCTCTGGTGAGCGGAGGTTTTCCACCGCCCGAGGAGGAGACACACAGTCAGCCAGTCAA TCCCCACAGCCTGCACCACCTGCATGCTGCCTACCGTGTCGGAATGCTGGCACTGGAGATGCTGGGTCGC CGGGCACACAACGATCACCCCAACAACTTCTCCCGCTCCCCCCCCTACACTGATGATGTCAAATGGTTGC TGGGGCTGGCAGCAAAGCTGGGAGTGAACTACGTGCACCAGTTCTGTGTGGGGGCAGCCAAGGGGGTGCT GAGCCCGTTTGTGCTGCAGGAGATCGTCATGGAGACGCTGCAGCGGCTGAGTCCCGCTCATGCCCACAAC CACCTGCGTGCCCCGGCCTTCCACCAACTGGTGCAGCGCTGCCAGCAGGCATACATGCAGTACATCCACC ACCGCTTGATTCACCTGACTCCTGCGGACTACGACGACTTTGTGAATGCGATCCGGAGTGCCCGCAGCGC CTTCTGCCTGACGCCCATGGGCATGATGCAGTTCAACGACATCCTACAGAACCTCAAGCGCAGCAAACAG ACCAAGGAGCTGTGGCAGCGGGTCTCACTCGAGATGGCCACCTTCTCCCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235338 representing NM_001242487
Red=Cloning site Green=Tags(s) MELMFAEWEDGERFSFEDSDRFEEDSLCSFISEAESLCQNWRGWRKQSAGPNSPTGGGGGGGSGGTRMRD GLVIPLVELSAKQVAFHIPFEVVEKVYPPVPEQLQLRIAFWSFPENEEDIRLYSCLANGSADEFQRGDQL FRMRAVKDPLQIGFHLSATVVPPQMVPPKGAYNVAVMFDRCRVTSCSCTCGAGAKWCTHVVALCLFRIHN ASAVCLRAPVSESLSRLQRDQLQKFAQYLISELPQQILPTAQRLLDELLSSQSTAINTVCGAPDPTAGPS ASDQSTWYLDESTLTDNIKKTLHKFCGPSPVVFSDVNSMYLSSTEPPAAAEWACLLRPLRGREPEGVWNL LSIVREMFKRRDSNAAPLLEILTDQCLTYEQITGWWYSVRTSASHSSASGHTGRSNGQSEVAAHACASMC DEMVTLWRLAVLDPALSPQRRRELCTQLRQWQLKVIENVKRGQHKKTLERLFPGFRPAVEACYFNWEEAY PLPGVTYSGTDRKLALCWARALPSRPGASRSGGLEESRDRPRPLPTEPAVRPKEPGTKRKGLGEGVPSSQ RGPRRLSAEGGDKALHKMGPGGGKAKALGGAGSGSKGSAGGGSKRRLSSEDSSLEPDLAEMSLDDSSLAL GAEASTFGGFPESPPPCPLHGGSRGPSTFLPEPPDTYEEDGGVYFSEGPEPPTASVGPPGLLPGDVCTQD DLPSTDESGNGLPKTKEAAPAVGEEDDDYQAYYLNAQDGAGGEEEKAEGGAGEEHDLFAGLKPLEQESRM EVLFACAEALHAHGYSSEASRLTVELAQDLLANPPDLKVEPPPAKGKKNKVSTSRQTWVATNTLSKAAFL LTVLSERPEHHNLAFRVGMFALELQRPPASTKALEVKLAYQESEVAALLKKIPLGPSEMSTMRCRAEELR EGTLCDYRPVLPLMLASFIFDVLCAPGSRPPSRNWNSETPGDEELGFEAAVAALGMKTTVSEAEHPLLCE GTRREKGDLALALMITYKDDQAKLKKILDKLLDRESQTHKPQTLSSFYSSSRPTTASQRSPSKHGGPSAP GALQPLTSGSAGPAQPGSVAGAGPGPTEGFTEKNVPESSPHSPCEGLPSEAALTPRPEGKVPSRLALGSR GGYNGRGWGSPGRPKKKHTGMASIDSSAPETTSDSSPTLSRRPLRGGWAPTSWGRGQDSDSISSSSSDSL GSSSSSGSRRASASGGARAKTVEVGRYKGRRPESHAPHVPNQPSEAAAHFYFELAKTVLIKAGGNSSTSI FTHPSSSGGHQGPHRNLHLCAFEIGLYALGLHNFVSPNWLSRTYSSHVSWITGQAMEIGSAALTILVECW DGHLTPPEVASLADRASRARDSNMVRAAAELALSCLPHAHALNPNEIQRALVQCKEQDNLMLEKACMAVE EAAKGGGVYPEVLFEVAHQWFWLYEQTAGGSSTAREGATSCSASGIRAGGEAGRGMPEGRGGPGTEPVTV AAAAVTAAATVVPVISVGSSLYPGPGLGHGHSPGLHPYTALQPHLPCSPQYLTHPAHPAHPMPHMPRPAV FPVPSSAYPQGVHPAFLGAQYPYSVTPPSLAATAVSFPVPSMAPITVHPYHTEPGLPLPTSVACELWGQG TVSSVHPASTFPAIQGASLPALTTQPSPLVSGGFPPPEEETHSQPVNPHSLHHLHAAYRVGMLALEMLGR RAHNDHPNNFSRSPPYTDDVKWLLGLAAKLGVNYVHQFCVGAAKGVLSPFVLQEIVMETLQRLSPAHAHN HLRAPAFHQLVQRCQQAYMQYIHHRLIHLTPADYDDFVNAIRSARSAFCLTPMGMMQFNDILQNLKRSKQ TKELWQRVSLEMATFSP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001242487 |
ORF Size | 5511 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001242487.2 |
RefSeq Size | 6062 bp |
RefSeq ORF | 5514 bp |
Locus ID | 23053 |
UniProt ID | A7E2V4 |
Gene Summary | Substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex that promotes target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs) (PubMed:33184234, PubMed:33184237). The SCF-like E3 ubiquitin-protein ligase complex acts by catalyzing ubiquitination and subsequent degradation of AGO proteins (AGO1, AGO2, AGO3 and/or AGO4), thereby exposing miRNAs for degradation (PubMed:33184234, PubMed:33184237). Specifically recognizes and binds AGO proteins when they are engaged with a TDMD target (PubMed:33184234). May also acts as a regulator of axon guidance: specifically recognizes misfolded ROBO3 and promotes its ubiquitination and subsequent degradation (PubMed:24012004).[UniProtKB/Swiss-Prot Function] |
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