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RTN3 (NM_001265590) Human Tagged ORF Clone
CAT＃: RG235038
- TrueORF®
RTN3 (tGFP-tagged) - Homo sapiens reticulon 3 (RTN3), transcript variant 6
ORF Plasmid: DDK
"NM_001265590" in other vectors (2)
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CNY 7,890.00
货期*
3周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ASYIP; HAP; NSPL2; NSPLII; RTN3-A1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235038 representing NM_001265590
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCCGTCGGCGGCCACTCAGTCCCATTCCATCTCCTCGTCGTCCTTCGGAGCCGAGCCGTCCG CGCCCGGCGGCGGCGGGAGCCCAGGAGCCTGCCCCGCCCTGGGGACGAAGAGCTGCAGCTCCTCCTGTGC GGCCAGTTTCCCAGAGCATCCTGCTTTTCTCTCAAAGAAAATTGGTCAAGTGGAAGAGCAAATAGATAAA GAGACCAAGAACCCAAATGGGGTATCAAGTAGGGAGGCTAAAACTGCATTGGATGCTGATGACAGATTCA CTTTGCTGACAGCCCAGAAACCACCTACTGAGTACTCTAAGGTAGAAGGCATTTATACATATTCTTTGTC TCCATCCAAAGTTTCAGGAGATGATGTTATTGAAAAGGATTCCCCTGAATCACCATTTGAAGTAATTATT GACAAAGCAGCATTTGACAAAGAATTTAAAGACTCATATAAGGAGAGCACAGATGATTTTGGTAGCTGGT CTGTGCACACTGATAAAGAATCATCCGAAGACATTTCAGAGACTAATGACAAGCTTTTTCCACTGAGAAA TAAAGAGGCAGGACGTTACCCAATGTCTGCATTGCTCAGTAGGCAGTTTTCACACACAAATGCAGCACTG GAAGAGGTGTCCAGATGCGTGAATGATATGCATAACTTTACTAACGAAATACTGACTTGGGATCTGGTTC CCCAAGTGAAACAACAGACCGATAAATCTTCTGACTGCATCACAAAAACTACAGGACTTGACATGAGTGA ATATAATTCAGAAATTCCAGTTGTAAATCTTAAAACTAGCACTCATCAGAAAACTCCTGTATGTTCTATT GATGGGAGCACTCCCATCACTAAATCAACAGGTGATTGGGCAGAAGCATCTCTCCAGCAAGAAAATGCTA TTACTGGAAAACCTGTACCTGACTCTTTGAATTCCACAAAAGAATTCAGTATCAAAGGTGTGCAAGGCAA TATGCAGAAACAGGATGACACACTTGCAGAATTACCTGGATCTCCACCTGAGAAATGTGACTCTTTGGGT TCTGGAGTGGCCACAGTGAAAGTGGTTTTACCTGATGACCACCTGAAAGATGAAATGGACTGGCAGAGCT CTGCATTGGGAGAAATCACAGAAGCTGATAGTTCTGGTGAGTCTGATGACACAGTAATAGAGGACATCAC AGCAGATACATCATTTGAAAATAACAAAATTCAGGCTGAAAAACCTGTTTCCATTCCAAGTGCTGTTGTA AAAACAGGTGAAAGAGAAATCAAAGAGATTCCCAGTTGTGAGAGAGAAGAAAAAACATCTAAAAACTTTG AAGAATTGGTCAGTGACTCTGAGCTGCATCAAGATCAGCCTGATATTCTTGGAAGGAGTCCAGCTAGTGA GGCAGCATGTTCAAAAGTACCCGATACGAATGTCTCCTTAGAAGATGTGAGTGAAGTTGCTCCTGAAAAG CCTATTACTACTGAGAACCCCAAACTTCCTTCAACAGTGTCTCCAAATGTTTTTAATGAGACAGAATTCT CATTAAATGTGACAACATCTGCCTATTTGGAGTCATTACATGGGAAAAATGTTAAACATATAGATGATTC CTCCCCAGAGGACCTGATAGCAGCCTTTACAGAAACCAGAGATAAAGGAATAGTAGATAGTGAAAGAAAT GCTTTTAAAGCAATATCAGAGAAGATGACAGACTTTAAAACAACTCCTCCTGTAGAAGTCTTACATGAAA ATGAGTCCGGTGGTTCTGAAATTAAAGACATTGGAAGCAAATACAGTGAACAAAGCAAAGAAACAAATGG AAGTGAGCCTCTAGGTGTTTTCCCTACCCAAGGTACTCCAGTAGCATCTCTTGACTTAGAACAAGAACAG CTCACAATTAAGGCTCTTAAAGAATTAGGTGAAAGACAGGTTGAGAAGTCAACTTCTGCACAGCGTGACG CAGAATTGCCTTCTGAAGAAGTACTGAAGCAAACTTTCACATTTGCTCCAGAATCTTGGCCACAGAGATC ATATGACATCCTAGAACGTAATGTCAAGAATGGATCTGATCTTGGGATTTCCCAGAAGCCCATCACTATC AGAGAAACTACTAGGGTAGATGCTGTTTCCAGCCTTAGCAAGACTGAATTGGTAAAAAAGCATGTCCTAG CAAGACTTCTGACAGACTTCTCAGTGCACGATCTGATTTTCTGGAGAGATGTGAAGAAGACTGGGTTTGT CTTTGGCACCACGCTGATCATGCTGCTTTCCCTGGCAGCTTTCAGTGTCATCAGTGTGGTTTCTTACCTC ATCCTGGCTCTTCTCTCTGTCACCATCAGCTTCAGGATCTACAAGTCCGTCATCCAAGCTGTACAGAAGT CAGAAGAAGGCCATCCATTCAAAGCCTACCTGGACGTAGACATTACTCTGTCCTCAGAAGCTTTCCATAA TTACATGAATGCTGCCATGGTGCACATCAACAGGGCCCTGAAACTCATTATTCGTCTCTTTCTGGTAGAA GATCTGGTTGACTCCTTGAAGCTGGCTGTCTTCATGTGGCTGATGACCTATGTTGGTGCTGTTTTTAACG GAATCACCCTTCTAATTCTTGCTGAACTGCTCATTTTCAGTGTCCCGATTGTCTATGAGAAGTACAAGAC CCAGATTGATCACTATGTTGGCATCGCCCGAGATCAGACCAAGTCAATTGTTGAAAAGATCCAAGCAAAA CTCCCTGGAATCGCCAAAAAAAAGGCAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235038 representing NM_001265590
Red=Cloning site Green=Tags(s) MAEPSAATQSHSISSSSFGAEPSAPGGGGSPGACPALGTKSCSSSCAASFPEHPAFLSKKIGQVEEQIDK ETKNPNGVSSREAKTALDADDRFTLLTAQKPPTEYSKVEGIYTYSLSPSKVSGDDVIEKDSPESPFEVII DKAAFDKEFKDSYKESTDDFGSWSVHTDKESSEDISETNDKLFPLRNKEAGRYPMSALLSRQFSHTNAAL EEVSRCVNDMHNFTNEILTWDLVPQVKQQTDKSSDCITKTTGLDMSEYNSEIPVVNLKTSTHQKTPVCSI DGSTPITKSTGDWAEASLQQENAITGKPVPDSLNSTKEFSIKGVQGNMQKQDDTLAELPGSPPEKCDSLG SGVATVKVVLPDDHLKDEMDWQSSALGEITEADSSGESDDTVIEDITADTSFENNKIQAEKPVSIPSAVV KTGEREIKEIPSCEREEKTSKNFEELVSDSELHQDQPDILGRSPASEAACSKVPDTNVSLEDVSEVAPEK PITTENPKLPSTVSPNVFNETEFSLNVTTSAYLESLHGKNVKHIDDSSPEDLIAAFTETRDKGIVDSERN AFKAISEKMTDFKTTPPVEVLHENESGGSEIKDIGSKYSEQSKETNGSEPLGVFPTQGTPVASLDLEQEQ LTIKALKELGERQVEKSTSAQRDAELPSEEVLKQTFTFAPESWPQRSYDILERNVKNGSDLGISQKPITI RETTRVDAVSSLSKTELVKKHVLARLLTDFSVHDLIFWRDVKKTGFVFGTTLIMLLSLAAFSVISVVSYL ILALLSVTISFRIYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHNYMNAAMVHINRALKLIIRLFLVE DLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLIFSVPIVYEKYKTQIDHYVGIARDQTKSIVEKIQAK LPGIAKKKAE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001265590 |
| ORF Size | 2760 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001265590.2 |
| RefSeq Size | 4640 bp |
| RefSeq ORF | 2763 bp |
| Locus ID | 10313 |
| UniProt ID | O95197 |
| Protein Families | Transmembrane |
| Gene Summary | This gene belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1 (BACE1), and the production of amyloid-beta. An increase in the expression of any reticulon protein substantially reduces the production of amyloid-beta, suggesting that reticulon proteins are negative modulators of BACE1 in cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, and pseudogenes of this gene are located on chromosomes 4 and 12. [provided by RefSeq, May 2012] |
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