CSDE1 (NM_001242892) Human Tagged ORF Clone
CAT#: RG234895
- TrueORF®
CSDE1 (tGFP-tagged) - Homo sapiens cold shock domain containing E1, RNA-binding (CSDE1), transcript variant 5
ORF Plasmid: DDK
"NM_001242892" in other vectors (2)
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CNY 6,370.00
CNY 6,281.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | D1S155E; UNR |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG234895 representing NM_001242892
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTTTGATCCAAACCTTCTCCACAACAATGGACATAATGGGTACCCTAATGGTACTTCAGCAGCAC TGCGTGAAACTGGGGTTATTGAAAAACTGTTAACCTCTTACGGATTTATTCAGTGTTCAGAACGTCAAGC TAGACTTTTCTTCCACTGTTCACAGTATAATGGCAACCTGCAAGACTTAAAAGTAGGAGATGATGTTGAA TTTGAAGTATCATCGGACCGACGGACTGGGAAACCCATTGCTGTTAAACTGGTGAAGATAAAACAAGAAA TCCTCCCTGAAGAACGAATGAATGGACAAGTTGTGTGCGCTGTTCCTCACAACTTAGAGAGTAAATCTCC AGCTGCCCCGGGTCAGAGTCCAACAGGGAGTGTATGCTACGAACGTAATGGGGAAGTGTTTTATCTGACT TACACCCCTGAAGATGTCGAAGGGAACGTTCAGCTGGAAACTGGAGATAAAATAAACTTTGTAATTGATA ACAATAAACATACTGGTGCTGTAAGTGCTCGCAACATTATGCTGTTGAAAAAGAAACAAGCCCGCTGTCA GGGAGTAGTTTGTGCCATGAAGGAGGCATTTGGCTTTATTGAAAGAGGTGATGTTGTAAAAGAGATATTC TTTCACTATAGTGAATTTAAGGGTGACTTAGAAACCTTACAGCCTGGCGATGATGTGGAATTCACAATCA AGGACAGAAATGGTAAAGAAGTTGCAACAGATGTCAGACTATTGCCTCAAGGAACAGTCATTTTTGAAGA TATCAGCATTGAACATTTTGAAGGAACTGTAACCAAAGTTATCCCAAAAGTACCCAGTAAAAACCAGAAT GACCCATTGCCAGGACGCATCAAAGTTGACTTTGTGATCCCTAAAGAACTTCCCTTTGGAGACAAAGATA CGAAATCCAAGGTGACCCTGCTGGAAGGTGACCATGTTAGGTTTAATATTTCAACAGACCGACGTGACAA ATTAGAGCGAGCAACCAATATAGAAGTTCTGTCAAATACATTTCAGTTCACTAATGAAGCCCGAGAAATG GGTGTGATTGCTGCCATGAGAGATGGTTTTGGTTTCATCAAGTGTGTGGATCGTGATGTTCGTATGTTCT TCCACTTCAGTGAAATTCTGGATGGGAACCAGCTCCATATTGCAGATGAAGTAGAGTTTACTGTGGTTCC TGATATGCTCTCTGCTCAAAGAAATCATGCTATTAGGATTAAAAAACTTCCCAAGGGCACGGTTTCATTT CATTCCCATTCAGATCACCGTTTTCTGGGCACGGTAGAAAAAGAAGCCACTTTTTCCAATCCTAAAACCA CTAGCCCAAATAAAGGCAAAGAGAAGGAGGCTGAGGATGGCATTATTGCTTATGATGACTGTGGGGTGAA ACTGACTATTGCTTTTCAAGCCAAGGATGTGGAAGGATCTACTTCTCCTCAAATAGGAGATAAGGTTGAA TTTAGTATTAGTGACAAACAGAGGCCTGGACAGCAGGTTGCAACTTGTGTGCGACTTTTAGGTCGTAATT CTAACTCCAAGAGGCTCTTGGGTTATGTGGCAACTCTGAAGGATAATTTTGGATTTATTGAAACAGCCAA TCATGATAAGGAAATCTTTTTCCATTACAGTGAGTTCTCTGGTGATGTTGATAGCCTGGAACTGGGGGAC ATGGTCGAGTATAGCTTGTCCAAAGGCAAAGGCAACAAAGTCAGTGCAGAAAAAGTGAACAAAACACACT CAGTGAATGGCATTACTGAGGAAGCTGATCCCACCATTTACTCTGGCAAAGTAATTCGCCCCCTGAGGAG TGTTGATCCAACACAGACTGAGTACCAAGGAATGATTGAGATTGTGGAGGAGGGCGATATGAAAGGTGAG GTCTATCCATTTGGCATCGTTGGGATGGCCAACAAAGGGGATTGCCTGCAGAAAGGGGAGAGCGTCAAGT TCCAATTGTGTGTCCTGGGCCAAAATGCACAAACTATGGCTTACAACATCACACCCCTGCGCAGGGCCAC AGTGGAATGTGTGAAAGATCAGTTTGGCTTCATTAACTATGAAGTAGGAGATAGCAAGAAGCTCTTTTTC CATGTGAAAGAAGTTCAGGATGGCATTGAGCTACAGGCAGGAGATGAGGTGGAGTTCTCAGTGATTCTTA ATCAGCGCACTGGCAAGTGCAGCGCCTGTAATGTTTGGCGAGTCTGTGAGGGCCCCAAGGCTGTTGCAGC TCCTCGACCTGATCGGTTGGTCAATCGCTTGAAGAATATCACTCTGGATGATGCCAGTGCTCCTCGCCTA ATGGTTCTTCGTCAGCCAAGGGGACCAGATAACTCAATGGGGTTTGGTGCAGAAAGAAAGATCCGTCAAG CTGGTGTCATTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234895 representing NM_001242892
Red=Cloning site Green=Tags(s) MSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSERQARLFFHCSQYNGNLQDLKVGDDVE FEVSSDRRTGKPIAVKLVKIKQEILPEERMNGQVVCAVPHNLESKSPAAPGQSPTGSVCYERNGEVFYLT YTPEDVEGNVQLETGDKINFVIDNNKHTGAVSARNIMLLKKKQARCQGVVCAMKEAFGFIERGDVVKEIF FHYSEFKGDLETLQPGDDVEFTIKDRNGKEVATDVRLLPQGTVIFEDISIEHFEGTVTKVIPKVPSKNQN DPLPGRIKVDFVIPKELPFGDKDTKSKVTLLEGDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREM GVIAAMRDGFGFIKCVDRDVRMFFHFSEILDGNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSF HSHSDHRFLGTVEKEATFSNPKTTSPNKGKEKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVE FSISDKQRPGQQVATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGD MVEYSLSKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRSVDPTQTEYQGMIEIVEEGDMKGE VYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTMAYNITPLRRATVECVKDQFGFINYEVGDSKKLFF HVKEVQDGIELQAGDEVEFSVILNQRTGKCSACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRL MVLRQPRGPDNSMGFGAERKIRQAGVID TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001242892 |
ORF Size | 2394 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001242892.2 |
RefSeq Size | 3796 bp |
RefSeq ORF | 2397 bp |
Locus ID | 7812 |
UniProt ID | O75534 |
Protein Families | Transcription Factors |
Gene Summary | RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |