ACSF3 (NM_001243279) Human Tagged ORF Clone
CAT#: RG234471
- TrueORF®
ACSF3 (tGFP-tagged) - Homo sapiens acyl-CoA synthetase family member 3 (ACSF3), transcript variant 4
ORF Plasmid: DDK
"NM_001243279" in other vectors (2)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG234471 representing NM_001243279
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCCCATGTGGTGCTCACCTTCCGGCGCCTGGGCTGCGCCTTGGCGTCCTGCCGGCTGGCGCCTG CGAGACACAGAGGAAGTGGTCTTCTGCACACAGCCCCAGTGGCCCGCTCGGACAGGAGCGCCCCGGTGTT CACCCGTGCCCTGGCCTTTGGGGACAGAATCGCCCTGGTTGACCAGCACGGCCGCCACACGTACAGGGAG CTTTATTCCCGCAGCCTTCGCCTGTCCCAGGAGATCTGCAGGCTCTGCGGGTGTGTCGGCGGGGACCTCC GGGAGGAGAGGGTCTCCTTCCTATGCGCTAACGATGCCTCCTACGTCGTGGCCCAGTGGGCGTCATGGAT GAGTGGCGGTGTGGCAGTCCCCCTCTACAGGAAGCATCCCGCGGCCCAGCTGGAGTATGTCATCTGCGAC TCCCAGAGCTCTGTGGTCCTTGCCAGCCAGGAGTACCTGGAGCTCCTGAGCCCGGTGGTCAGGAAGCTGG GGGTCCCGCTGCTGCCGCTCACACCAGCCATCTACACTGGAGCAGTAGAGGAACCGGCAGAGGTCCCGGT CCCAGAGCAGGGATGGAGGAACAAGGGCGCCATGATCATCTACACCAGTGGGACCACGGGGAGGCCCAAG GGCGTGCTGAGCACGCACCAAAACATCAGGGCTGTGGTGACCGGGCTGGTCCACAAGTGGGCATGGACCA AAGACGACGTGATCCTCCACGTGCTCCCGCTGCACCACGTCCATGGTGTGGTCAACGCGCTGCTCTGTCC TCTCTGGGTGGGAGCCACCTGTGTGATGATGCCTGAGTTCAGCCCTCAGCAGGTTTGGGAAAAGTTCTTA AGTTCTGAAACGCCGCGGATCAATGTCTTTATGGCAGTGCCTACAATATACACCAAGCTGATGGAGTACT ACGACAGGCATTTTACCCAGCCGCACGCCCAGGATTTCTTGCGTGCAGTTTGTGAAGAAAAAATTAGGCT GATGGTCTCAGGCTCAGCTGCCCTGCCCCTCCCAGTGCTGGAGAAGTGGAAGAACATCACGGGCCACACC CTGCTGGAGCGGTATGGCATGACCGAGATCGGCATGGCTCTGTCCGGGCCCCTGACCACTGCCGTGCGCC TGCCAGGTTCCGTGGGGACCCCACTGCCTGGAGTACAGGTGCGCATTGTCTCAGAAAACCCACAGAGGGA AGCCTGCTCCTACACCATCCACGCAGAGGGAGACGAGAGGGGGACCAAGGTGACCCCAGGGTTTGAAGAA AAGGAGGGGGAGCTGCTGGTGAGGGGACCCTCCGTGTTTCGAGAATACTGGAATAAACCAGAAGAAACTA AGAGTGCATTCACCCTGGATGGCTGGTTTAAGACAGGGGACACCGTGGTGTTTAAGGATGGCCAGTACTG GATCCGAGGCCGGACCTCAGTGGACATCATCAAGACTGGAGGCTACAAGGTCAGCGCCCTGGAGGTGGAG TGGCACCTGCTGGCCCACCCCAGCATCACAGATGTGGCTGTGATTGGAGTTCCGGATATGACATGGGGCC AGCGGGTCACTGCTGTGGTGACCCTCCGAGAAGGACACTCACTGTCCCACAGGGAGCTCAAAGAGTGGGC CAGAAATGTCCTGGCCCCGTACGCGGTGCCCTCGGAGCTGGTGCTGGTGGAGGAGATCCCGCGGAACCAG ATGGGCAAGATTGACAAGAAGGCGCTCATCAGGCACTTCCACCCCTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234471 representing NM_001243279
Red=Cloning site Green=Tags(s) MLPHVVLTFRRLGCALASCRLAPARHRGSGLLHTAPVARSDRSAPVFTRALAFGDRIALVDQHGRHTYRE LYSRSLRLSQEICRLCGCVGGDLREERVSFLCANDASYVVAQWASWMSGGVAVPLYRKHPAAQLEYVICD SQSSVVLASQEYLELLSPVVRKLGVPLLPLTPAIYTGAVEEPAEVPVPEQGWRNKGAMIIYTSGTTGRPK GVLSTHQNIRAVVTGLVHKWAWTKDDVILHVLPLHHVHGVVNALLCPLWVGATCVMMPEFSPQQVWEKFL SSETPRINVFMAVPTIYTKLMEYYDRHFTQPHAQDFLRAVCEEKIRLMVSGSAALPLPVLEKWKNITGHT LLERYGMTEIGMALSGPLTTAVRLPGSVGTPLPGVQVRIVSENPQREACSYTIHAEGDERGTKVTPGFEE KEGELLVRGPSVFREYWNKPEETKSAFTLDGWFKTGDTVVFKDGQYWIRGRTSVDIIKTGGYKVSALEVE WHLLAHPSITDVAVIGVPDMTWGQRVTAVVTLREGHSLSHRELKEWARNVLAPYAVPSELVLVEEIPRNQ MGKIDKKALIRHFHPS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001243279 |
ORF Size | 1728 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001243279.3 |
RefSeq Size | 3751 bp |
RefSeq ORF | 1731 bp |
Locus ID | 197322 |
UniProt ID | Q4G176 |
Gene Summary | This gene encodes a member of the acyl-CoA synthetase family of enzymes that activate fatty acids by catalyzing the formation of a thioester linkage between fatty acids and coenzyme A. The encoded protein is localized to mitochondria, has high specificity for malonate and methylmalonate and possesses malonyl-CoA synthetase activity. Mutations in this gene are a cause of combined malonic and methylmalonic aciduria. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Sep 2013] |
Documents
Product Manuals |
FAQs |
SDS |