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DDX19B (NM_001257172) Human Tagged ORF Clone
CAT＃: RG234146
- TrueORF®
DDX19B (tGFP-tagged) - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B (DDX19B), transcript variant 4
ORF Plasmid: DDK
"NM_001257172" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DBP5; DDX19; RNAh |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234146 representing NM_001257172
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGGCTGCCGGGCGCGTCCAAGATCGCGCCCTGCGGCGGTTTCCCATCACCCTGCCTGTGGGCG ACTTGAGCAACTTGCATCTTAAGGAAGAGAAAATCAAACCAGATACCAATGGTGCTGTTGTCAAGACCAA TGCCAATGCAGAGAAGACAGATGAAGAAGAGAAAGAGGACAGAGCTGCCCAGTCCTTACTCAACAAGCTG ATCAGAAGCAACCTTGTTGATAACACAAACCAAGTGGAAGTCCTGCAGCGGGATCCAAACTCCCCTCTGT ACTCGGTGAAGTCTTTTGAAGAGCTTCGGCTCCCACAGAACTTAATTGCCCAATCTCAGTCTGGTACTGG TAAAACAGCTGCCTTCGTGCTGGCCATGCTTAGCCAAGTAGAACCTGCAAACAAATACCCCCAGTGTCTA TGTCTCTCCCCAACGTATGAGCTCGCCCTCCAAACAGGAAAAGTGATTGAACAAATGGGCAAATTTTACC CTGAACTGAAGCTAGCTTATGCTGTTCGAGGCAATAAATTGGAAAGAGGCCAGAAGATCAGTGAGCAGAT TGTCATTGGCACCCCTGGGACTGTGCTGGACTGGTGCTCCAAGCTCAAGTTCATTGATCCCAAGAAAATC AAGGTGTTTGTTCTGGATGAGGCTGATGTCATGATAGCCACTCAGGGCCACCAAGATCAGAGCATCCGCA TCCAGAGGATGCTGCCCAGGAACTGCCAGATGCTGCTTTTCTCCGCCACCTTTGAAGACTCTGTGTGGAA GTTTGCCCAGAAAGTGGTCCCAGACCCAAACGTTATCAAACTGAAGCGTGAGGAAGAGACCCTGGACACC ATCAAGCAGTACTATGTCCTGTGCAGCAGCAGAGACGAGAAGTTCCAGGCCTTGTGTAACCTCTACGGGG CCATCACCATTGCTCAAGCCATGATCTTCTGCCATACTCGCAAAACAGCTAGTTGGCTGGCAGCAGAGCT CTCAAAAGAAGGCCACCAGGTGGCTCTGCTGAGTGGGGAGATGATGGTGGAACAGAGGGCTGCAGTGATT GAGCGCTTCCGAGAGGGCAAAGAGAAGGTTTTGGTGACCACCAACGTGTGTGCCCGCGGCATTGATGTTG AACAAGTGTCTGTCGTCATCAACTTTGATCTTCCCGTGGACAAGGACGGGAATCCTGACAATGAGACCTA CCTGCACCGGATCGGGCGCACGGGCCGCTTTGGCAAGAGGGGCCTGGCAGTGAACATGGTGGACAGCAAG CACAGCATGAACATCCTGAACAGAATCCAGGAGCATTTTAATAAGAAGATAGAAAGATTGGACACAGATG ATTTGGACGAGATTGAGAAAATAGCCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234146 representing NM_001257172
Red=Cloning site Green=Tags(s) MAGAAGRVQDRALRRFPITLPVGDLSNLHLKEEKIKPDTNGAVVKTNANAEKTDEEEKEDRAAQSLLNKL IRSNLVDNTNQVEVLQRDPNSPLYSVKSFEELRLPQNLIAQSQSGTGKTAAFVLAMLSQVEPANKYPQCL CLSPTYELALQTGKVIEQMGKFYPELKLAYAVRGNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKKI KVFVLDEADVMIATQGHQDQSIRIQRMLPRNCQMLLFSATFEDSVWKFAQKVVPDPNVIKLKREEETLDT IKQYYVLCSSRDEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLSGEMMVEQRAAVI ERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFGKRGLAVNMVDSK HSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001257172 |
| ORF Size | 1359 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001257172.2 |
| RefSeq Size | 1661 bp |
| RefSeq ORF | 1362 bp |
| Locus ID | 11269 |
| UniProt ID | Q9UMR2 |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
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