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ZNF451 (NM_001257273) Human Tagged ORF Clone
CAT＃: RG232971
- TrueORF®
ZNF451 (tGFP-tagged) - Homo sapiens zinc finger protein 451 (ZNF451), transcript variant 3
ORF Plasmid: DDK
"NM_001257273" in other vectors (2)
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CNY 5,040.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | COASTER; dJ417I1.1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232971 representing NM_001257273
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGACCCGGGGTCGGAGATAATAGAATCTGTCCCTCCAGCTGGCCCTGAGGCATCTGAGTCAACAA CGGATGAAAATGAAGACGACATTCAGTTTGTCAGTGAAGGACCATTACGACCTGTTCTTGAATACATTGA TCTGGTCAGCAGTGATGATGAAGAGCCTAGCACCTCTTATACTGATAGAATGCCTGAATCTAAGGTGCCA TCCTCTGAGAATCATCGCCCAGAAATGTGCTCTAGCTGCAATGTTCCTCTTCCCATTGGAGATAGCAGCT CCTTCTCTGGGAGTTGTTCCAGCAGTCCAGAAAGGATAGTTTCTCAAACTTCCTCTGTTGAGAACCCATT GGAGAACCAGAAAAATGATCAAAATAATTCAGATACTAAGATCTCTGAGACAGAGACCCTTAAATCATCA CAGAATTTTCAGACTCTGCCTTCATCTCCACTTCTGGTCCCCCAAGAATCTTTGGCCTCTTCTGAGGTCA AAGAGAATTTACGTATAGATTCTTCTTCAGCTTCACAGCATGGACGGGATGCCATCCTCTATCTCCAGAC ACAAGTAGCTGAAATGTCCCGAGTGATACGTGATCTGCAGTCCAGGAGCTGTTTTAGATTTCATCATTCT AGGCCAAGTGAGAACTCCTCAGTTCCTTGGGACATCTCCACCTCTAAAGAGGAAAATTTATCCACAGTTG AAGAAGAAACTGATTACAAATCACCATCAGCTGATGACAAAGGGCAGCCATCTGACCCCAGCCAATCTAG TTTCACAGGTCTTTTGAAGAGAATGGAACAAAGAGGTGTTATAAAAAGGGTGACATTACAATCTGAAGCG GAGTCATGTGAAGGGAAACCTGATTGTGTGACTTCTAAAAAACGTTTGGTTCCTCCATTGCATCCTCTTC TGAGAATTGCCACCACTGAGGTTTTTAAAGACCCTGCTGATTGCCATCCTTCTTCCTTCATGGGACACAG GGTATATCCTGTGGCCAAGGACACCTCTCCTTTCCAACCAAACCCACCAGCTGAAGGCCCCATTGTAGAA GCATTAGAACACAGCAAAAGAGGAAACACAACATCCCCTCTAGATTCTACCTCAAAAGAAATGGAGGTCA TGGGTTGTAGGTTTTACCACGCTGCCTCCATTGCAGCCCGAGCTGCTAGCTACATGGCCTATATGACTCA ATATCAGCGTAAACTCTGGGAAGACATGGAAGATCTGGTTCATGACCCAGAATTTGATCGTGGAAAAGCA AGATGCATAATATCTGATGGTATGGATGCAGGCCTTTGGCAACTTTGTACTACTAGGGACATAATGGACT CTGTAGTCAGAGTTATGGCCATGGCCATAGACTATAGACGGCAGGCCTGGCTTCGACTTACATCTCTCAC TAAGAAAACCCAGGAGAAGATCTCCCACTTGCCCTTTGATGGTACTTCCCTTTTTGGACAAGATGTGAAA GCTGTTGTTGCAGAAGACAACAATATAAAAGAAAATGACTATAAAGATCACAAATACTATAATCAGCATC GATACTTTTATAGTCATGATCAGAAAGCACATTATCACAATAGAGGATACTCCAAAGGGGATTGGTACAA ACCTCGAAACCACCCCTATAGATATAGAAAGAAGGGAGACTCTCCAGAACGCCATGGGTACAAGAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232971 representing NM_001257273
Red=Cloning site Green=Tags(s) MGDPGSEIIESVPPAGPEASESTTDENEDDIQFVSEGPLRPVLEYIDLVSSDDEEPSTSYTDRMPESKVP SSENHRPEMCSSCNVPLPIGDSSSFSGSCSSSPERIVSQTSSVENPLENQKNDQNNSDTKISETETLKSS QNFQTLPSSPLLVPQESLASSEVKENLRIDSSSASQHGRDAILYLQTQVAEMSRVIRDLQSRSCFRFHHS RPSENSSVPWDISTSKEENLSTVEEETDYKSPSADDKGQPSDPSQSSFTGLLKRMEQRGVIKRVTLQSEA ESCEGKPDCVTSKKRLVPPLHPLLRIATTEVFKDPADCHPSSFMGHRVYPVAKDTSPFQPNPPAEGPIVE ALEHSKRGNTTSPLDSTSKEMEVMGCRFYHAASIAARAASYMAYMTQYQRKLWEDMEDLVHDPEFDRGKA RCIISDGMDAGLWQLCTTRDIMDSVVRVMAMAIDYRRQAWLRLTSLTKKTQEKISHLPFDGTSLFGQDVK AVVAEDNNIKENDYKDHKYYNQHRYFYSHDQKAHYHNRGYSKGDWYKPRNHPYRYRKKGDSPERHGYKN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001257273 |
| ORF Size | 1677 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001257273.2 |
| RefSeq Size | 9558 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 26036 |
| Protein Families | Transcription Factors |
| Gene Summary | E3 SUMO-protein ligase; has a preference for SUMO2 and SUMO3 and facilitates UBE2I/UBC9-mediated sumoylation of target proteins (PubMed:26524493, PubMed:26524494). Plays a role in protein SUMO2 modification in response to stress caused by DNA damage and by proteasome inhibitors (in vitro). Required for MCM4 sumoylation (By similarity). Has no activity with SUMO1 (PubMed:26524493). Preferentially transfers an additional SUMO2 chain onto the SUMO2 consensus site 'Lys-11' (PubMed:26524493). Negatively regulates transcriptional activation mediated by the SMAD4 complex in response to TGF-beta signaling. Inhibits EP300-mediated acetylation of histone H3 at 'Lys-9' (PubMed:24324267). Plays a role in regulating the transcription of AR targets (PubMed:18656483).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
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