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CCDC51 (NM_001256964) Human Tagged ORF Clone
CAT＃: RG232677
- TrueORF®
CCDC51 (tGFP-tagged) - Homo sapiens coiled-coil domain containing 51 (CCDC51), transcript variant 1
ORF Plasmid: DDK
"NM_001256964" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MITOK |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232677 representing NM_001256964
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGGGCGCAGCCCTGGGTTTGCCATGCAGCACATCGTGGGTGTGCCCCACGTACTGGTTCGGAGGG GCCTCCTTGGAAGGGACCTCTTCATGACCAGGACTCTCTGCAGCCCAGGCCCAAGCCAGCCCGGAGAGAA AAGACCTGAGGAGGTGGCCCTGGGGCTGCACCACCGCCTCCCAGCACTGGGAAGAGCCCTGGGGCACAGC ATTCAGCAACGAGCGACCTCCACAGCCAAGACTTGGTGGGACAGATATGAAGAGTTTGTTGGACTCAACG AGGTTCGAGAGGCCCAGGGAAAGGTGACAGAGGCTGAGAAAGTGTTCATGGTGGCTCGAGGGCTTGTCCG AGAGGCTCGGGAGGACTTGGAAGTTCACCAGGCCAAGCTGAAGGAGGTGAGGGACCGCTTGGACCGTGTC TCCAGGGAGGACAGTCAGTACTTGGAACTGGCTACTCTCGAGCACAGGATGCTGCAGGAGGAGAAGAGGC TTCGCACAGCCTATCTGCGTGCAGAAGACTCTGAGCGAGAGAAGTTCTCCCTCTTCTCTGCAGCTGTGCG GGAAAGTCATGAGAAGGAGCGCACAAGGGCTGAGAGGACCAAGAACTGGTCCCTCATTGGCTCAGTCCTG GGGGCCCTGATTGGTGTGGCTGGCTCCACCTATGTGAACCGTGTGCGACTACAGGAGCTGAAGGCTTTAC TCCTGGAGGCGCAGAAGGGGCCTGTGAGTCTCCAAGAGGCCATTCGAGAACAGGCGTCTAGCTACTCCCG CCAGCAGAGGGACCTCCACAATCTCATGGTGGACTTGAGGGGCCTGGTACATGCTGCTGGGCCAGGGCAG GACTCTGGGTCACAGGCAGGTAGTCCCCCGACCAGAGACAGAGATGTAGATGTCCTTTCAGCTGCCTTGA AAGAGCAGCTTAGTCATTCCAGGCAAGTCCATTCATGTCTAGAAGGCTTACGAGAGCAGCTTGATGGCCT AGAAAAGACTTGTAGCCAAATGGCTGGGGTGGTTCAGCTTGTAAAGTCTGCAGCACACCCAGGCCTGGTG GAACCAGCAGACGGGGCTATGCCCAGCTTCTTGCTGGAGCAGGGGAGCATGATCTTGGCACTGTCAGACA CGGAGCAGAGACTAGAAGCCCAAGTCAACAGGAACACCATCTATAGCACCCTGGTCACCTGTGTGACATT TGTGGCCACACTGCCTGTGCTCTACATGCTATTCAAAGCCAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232677 representing NM_001256964
Red=Cloning site Green=Tags(s) MMGRSPGFAMQHIVGVPHVLVRRGLLGRDLFMTRTLCSPGPSQPGEKRPEEVALGLHHRLPALGRALGHS IQQRATSTAKTWWDRYEEFVGLNEVREAQGKVTEAEKVFMVARGLVREAREDLEVHQAKLKEVRDRLDRV SREDSQYLELATLEHRMLQEEKRLRTAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVL GALIGVAGSTYVNRVRLQELKALLLEAQKGPVSLQEAIREQASSYSRQQRDLHNLMVDLRGLVHAAGPGQ DSGSQAGSPPTRDRDVDVLSAALKEQLSHSRQVHSCLEGLREQLDGLEKTCSQMAGVVQLVKSAAHPGLV EPADGAMPSFLLEQGSMILALSDTEQRLEAQVNRNTIYSTLVTCVTFVATLPVLYMLFKAS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001256964 |
| ORF Size | 1233 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001256964.1, NP_001243893.1 |
| RefSeq Size | 1627 bp |
| RefSeq ORF | 1236 bp |
| Locus ID | 79714 |
| UniProt ID | Q96ER9 |
| Protein Families | Transmembrane |
| Gene Summary | Mitochondrial potassium channel located in the mitochondrial inner membrane (PubMed:31435016). Together with ABCB8/MITOSUR, forms a protein complex localized in the mitochondria that mediates ATP-dependent potassium currents across the inner membrane (that is, mitoK(ATP) channel) (PubMed:31435016). May contribute to the homeostatic control of cellular metabolism under stress conditions by regulating the mitochondrial matrix volume (PubMed:31435016).[UniProtKB/Swiss-Prot Function] |
Documents
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