DEFB119 (NM_001271209) Human Tagged ORF Clone
CAT#: RG231596
- TrueORF®
DEFB119 (tGFP-tagged) - Homo sapiens defensin, beta 119 (DEFB119), transcript variant 4
ORF Plasmid: DDK
"NM_001271209" in other vectors (2)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DEFB-19; DEFB-20; DEFB20; DEFB120; ESC42-RELA; ESC42-RELB |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG231596 representing NM_001271209
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACTTCTTTACCTGTTTCTTGCCATCCTTCTGGCCATAGAAGAACCAGTGATATCAGAGTGTTGGA TGGATGGACACTGCCGGTTGTTGTGCAAAGATGGTGAAGACAGCATCATACGCTGCCGAAATCGTAAACG GTGCTGTGTTCCTAGTCGTTATTTAACAATCCAACCAGTAACAATTCATGGAATCCTTGGCTGGACCACT CCTCAGATGTCCACAACAGCTCCAAAAATGAAGACAAATATAACTAATAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231596 representing NM_001271209
Red=Cloning site Green=Tags(s) MKLLYLFLAILLAIEEPVISECWMDGHCRLLCKDGEDSIIRCRNRKRCCVPSRYLTIQPVTIHGILGWTT PQMSTTAPKMKTNITNR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001271209 |
ORF Size | 261 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001271209.2 |
RefSeq Size | 510 bp |
RefSeq ORF | 264 bp |
Locus ID | 245932 |
UniProt ID | Q8N690 |
Protein Families | Secreted Protein |
Gene Summary | This gene encodes a member of the beta subfamily of defensins. Beta-defensins are antimicrobial peptides that protect tissues and organs from infection by a variety of microorganisms. This gene is found in a cluster with other beta-defensin genes on the long arm of chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014] |
Documents
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Resources
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