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DGCR8 (NM_001190326) Human Tagged ORF Clone
CAT＃: RG230872
- TrueORF®
DGCR8 (tGFP-tagged) - Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 2
ORF Plasmid: DDK
"NM_001190326" in other vectors (3)
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CNY 8,170.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C22orf12; DGCRK6; Gy1; pasha |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230872 representing NM_001190326
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACAGATGAGAGCCCCTCTCCGCTCCCGTGTGGGCCCGCAGGAGAAGCGGTGATGGAGAGCCGAG CTCGCCCCTTCCAAGCGCTGCCCCGTGAGCAGTCTCCACCACCTCCCCTGCAAACGTCCAGTGGTGCAGA GGTAATGGACGTTGGCTCTGGTGGTGATGGACAGTCCGAACTCCCTGCTGAGGACCCCTTCAACTTCTAC GGAGCTTCTCTTCTCTCCAAAGGATCCTTCTCTAAGGGCCGCCTCCTCATAGACCCGAACTGTAGTGGCC ACAGCCCGCGCACCGCCCGGCACGCACCTGCGGTCCGGAAGTTCTCCCCTGACCTTAAGTTGCTTAAGGA TGTAAAGATTAGCGTGAGCTTTACCGAGAGCTGCAGGAGTAAGGACAGGAAGGTGCTGTACACAGGAGCA GAGCGCGACGTGCGGGCGGAGTGCGGTCTGCTCCTTAGCCCTGTCAGTGGGGACGTGCATGCTTGTCCCT TTGGCGGGAGTGTTGGTGACGGGGTAGGCATAGGGGGTGAGAGTGCTGATAAGAAGGATGAGGAGAATGA GCTGGATCAGGAAAAGAGAGTGGAGTATGCAGTGCTCGATGAGTTAGAAGATTTTACTGACAATTTGGAG CTAGATGAAGAAGGAGCAGGCGGGTTCACGGCTAAAGCAATCGTTCAGAGAGACAGAGTGGATGAAGAGG CCTTGAATTTCCCCTACGAGGATGACTTTGACAACGATGTGGATGCTCTGCTGGAAGAAGGCCTTTGTGC CCCCAAAAAGAGGCGAACAGAGGAAAAATATGGCGGAGACAGCGACCATCCGTCCGATGGAGAGACAAGT GTGCAGCCGATGATGACCAAGATTAAAACAGTGCTCAAAAGTCGTGGCCGCCCACCTACAGAGCCGCTGC CCGACGGGTGGATCATGACATTCCATAACTCTGGAGTCCCGGTGTACCTACACAGAGAGTCTCGGGTGGT CACCTGGTCCAGGCCATACTTCTTGGGAACGGGAAGCATACGGAAACACGACCCTCCTCTGAGTAGCATC CCTTGTCTGCATTATAAGAAAATGAAGGACAACGAGGAACGGGAGCAAAGCAGTGACCTCACCCCTAGTG GGGATGTGTCCCCCGTCAAGCCCCTGAGCCGATCTGCAGAGCTGGAGTTTCCCCTGGATGAGCCTGACTC TATGGGTGCTGACCCGGGGCCCCCGGACGAGAAAGACCCACTAGGGGCTGAGGCAGCCCCTGGGGCCCTG GGGCAGGTGAAGGCCAAAGTCGAGGTGTGCAAAGATGAATCCGTTGATCTCGAGGAATTTCGAAGCTACC TGGAGAAGCGTTTTGACTTTGAGCAAGTTACTGTGAAAAAATTCAGGACTTGGGCTGAGCGGCGGCAATT CAATCGGGAAATGAAGCGGAAGCAGGCGGAGTCCGAGAGGCCCATCTTGCCAGCCAATCAGAAGCTCATT ACTTTATCAGTGCAAGATGCACCCACAAAGAAAGAGTTTGTTATTAACCCCAACGGGAAATCCGAGGTCT GCATCCTGCACGAGTACATGCAGCGTGTCCTCAAGGTCCGCCCTGTCTATAATTTCTTTGAATGTGCCCG AGCTACACTGGAAATCCTCATCCCTGACTTTGTTAAACAGACCTCTGAAGAGAAGCCCAAAGACAGTGAA GAACTCGAGTATTTTAACCACATCAGCATCGAGGACTCGCGGGTCTACGAGCTGACCAGCAAGGCTGGGC TGTTGTCTCCATATCAGATCCTCCACGAGTGCCTTAAAAGAAACCATGGGATGGGTGACACGTCTATCAA GTTTGAAGTGGTTCCTGGGAAAAACCAGAAGAGTGAATACGTCATGGCGTGTGGCAAGCACACAGTGCGC GGGTGGTGTAAGAACAAGAGAGTTGGAAAGCAGTTAGCCTCACAGAAGATCCTTCAGCTGCTGCACCCAC ATGTCAAGAACTGGGGGTCTTTACTGCGCATGTATGGCCGTGAGAGCAGCAAGATGGTCAAGCAGGAGAC ATCGGACAAGAGTGTGATTGAGCTGCAGCAGTATGCCAAGAAGAACAAGCCCAACCTGCACATCCTCAGC AAGCTCCAAGAGGAGATGAAGAGGCTAGCTGAGGAAAGGGAGGAGACTCGAAAGAAGCCCAAGATGTCCA TTGTGGCGTCCGCCCAGCCTGGCGGTGAGCCCCTGTGCACCGTGGACGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230872 representing NM_001190326
Red=Cloning site Green=Tags(s) METDESPSPLPCGPAGEAVMESRARPFQALPREQSPPPPLQTSSGAEVMDVGSGGDGQSELPAEDPFNFY GASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGA ERDVRAECGLLLSPVSGDVHACPFGGSVGDGVGIGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLE LDEEGAGGFTAKAIVQRDRVDEEALNFPYEDDFDNDVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETS VQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSI PCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMGADPGPPDEKDPLGAEAAPGAL GQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLI TLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECARATLEILIPDFVKQTSEEKPKDSE ELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVR GWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILS KLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001190326 |
| ORF Size | 2220 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001190326.1, NP_001177255.1 |
| RefSeq Size | 4437 bp |
| RefSeq ORF | 2223 bp |
| Locus ID | 54487 |
| UniProt ID | Q8WYQ5 |
| Gene Summary | This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230872 | DGCR8 (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 2 |
CNY 7,410.00 |
|
| RC230872L3 | Lenti-ORF clone of DGCR8 (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 2 |
CNY 9,310.00 |
|
| RC230872L4 | Lenti-ORF clone of DGCR8 (mGFP-tagged)-Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 2 |
CNY 9,310.00 |
