IKBKE (NM_001193321) Human Tagged ORF Clone
CAT#: RG230859
- TrueORF®
IKBKE (tGFP-tagged) - Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE), transcript variant 3
ORF Plasmid: DDK
"NM_001193321" in other vectors (3)
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CNY 6,940.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | IKK-E; IKK-i; IKKE; IKKI |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG230859 representing NM_001193321
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTACTGCTCCAGTGGGAGCCTGCTGAGTGTGCTGGAGAGCCCTGAGAATGCCTTTGGGCTGCCTG AGGATGAGTTCCTGGTGGTGCTGCGCTGTGTGGTGGCCGGCATGAACCACCTGCGGGAGAACGGCATTGT GCATCGCGACATCAAGCCGGGGAACATCATGCGCCTCGTAGGGGAGGAGGGGCAGAGCATCTACAAGCTG ACAGACTTCGGCGCTGCCCGGGAGCTGGATGATGATGAGAAGTTCGTCTCGGTCTATGGGACTGAGGAGT ACCTGCATCCCGACATGTATGAGCGGGCGGTGCTTCGAAAGCCCCAGCAAAAAGCGTTCGGGGTGACTGT GGATCTCTGGAGCATTGGAGTGACCTTGTACCATGCAGCCACTGGCAGCCTGCCCTTCATCCCCTTTGGT GGGCCACGGCGGAACAAGGAGATCATGTACCGGATCACCACGGAGAAGCCGGCTGGGGCCATTGCAGGTG CCCAGAGGCGGGAGAACGGGCCCCTGGAGTGGAGCTACACCCTCCCCATCACCTGCCAGCTGTCACTGGG GCTGCAGAGCCAGCTGGTGCCCATCCTGGCCAACATCCTGGAGGTGGAGCAGGCCAAGTGCTGGGGCTTC GACCAGTTCTTTGCGGAGACCAGTGACATCCTGCAGCGAGTTGTCGTCCATGTCTTCTCCCTGTCCCAGG CAGTCCTGCACCACATCTATATCCATGCCCACAACACGATAGCCATTTTCCAGGAGGCCGTGCACAAGCA GACCAGTGTGGCCCCCCGACACCAGGAGTACCTCTTTGAGGGTCACCTCTGTGTCCTCGAGCCCAGCGTC TCAGCACAGCACATCGCCCACACGACGGCAAGCAGCCCCCTGACCCTCTTCAGCACAGCCATCCCTAAGG GGCTGGCCTTCAGGGACCCTGCTCTGGACGTCCCCAAGTTCGTCCCCAAAGTGGACCTGCAGGCGGATTA CAACACTGCCAAGGGCGTGTTGGGCGCCGGCTACCAGGCCCTGCGGCTGGCACGGGCCCTGCTGGATGGG CAGGAGCTAATGTTTCGGGGGCTGCACTGGGTCATGGAGGTGCTCCAGGCCACATGCAGACGGACTCTGG AAGTGGCAAGGACATCCCTCCTCTACCTCAGCAGCAGCCTGGGAACTGAGAGGTTCAGCAGCGTGGCTGG AACGCCTGAGATCCAGGAACTGAAGGCGGCTGCAGAACTGAGGTCCAGGCTGCGGACTCTAGCGGAGGTC CTCTCCAGATGCTCCCAAAATATCACGGAGACCCAGGAGAGCCTGAGCAGCCTGAACCGGGAGCTGGTGA AGAGCCGGGATCAGGTACATGAGGACAGAAGCATCCAGCAGATTCAGTGCTGTTTGGACAAGATGAACTT CATCTACAAACAGTTCAAGAAGTCTAGGATGAGGCCAGGGCTTGGCTACAACGAGGAGCAGATTCACAAG CTGGATAAGGTGAATTTCAGTCATTTAGCCAAAAGACTCCTGCAGGTGTTCCAGGAGGAGTGCGTGCAGA AGTATCAAGCGTCCTTAGTCACACACGGCAAGAGGATGAGGGTGGTGCACGAGACCAGGAACCACCTGCG CCTGGTTGGCTGTTCTGTGGCTGCCTGTAACACAGAAGCCCAGGGGGTCCAGGAGAGTCTCAGCAAGCTC CTGGAAGAGCTATCTCACCAGCTCCTTCAGGACCGAGCAAAGGGGGCTCAGGCCTCGCCGCCTCCCATAG CTCCTTACCCCAGCCCTACACGAAAGGACCTGCTTCTCCACATGCAAGAGCTCTGCGAGGGGATGAAGCT GCTGGCATCTGACCTCCTGGACAACAACCGCATCATCGAACGGCTAAATAGAGTCCCAGCACCTCCTGAT GTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230859 representing NM_001193321
Red=Cloning site Green=Tags(s) MEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKL TDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFG GPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPILANILEVEQAKCWGF DQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSV SAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDG QELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQELKAAAELRSRLRTLAEV LSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHK LDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGVQESLSKL LEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCEGMKLLASDLLDNNRIIERLNRVPAPPD V TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001193321 |
ORF Size | 1893 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001193321.2 |
RefSeq Size | 3373 bp |
RefSeq ORF | 1896 bp |
Locus ID | 9641 |
UniProt ID | Q14164 |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway |
Gene Summary | IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC230859 | IKBKE (Myc-DDK-tagged)-Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE), transcript variant 3 |
CNY 6,270.00 |
|
RC230859L3 | Lenti-ORF clone of IKBKE (Myc-DDK-tagged)-Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE), transcript variant 3 |
CNY 8,170.00 |
|
RC230859L4 | Lenti-ORF clone of IKBKE (mGFP-tagged)-Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE), transcript variant 3 |
CNY 8,170.00 |