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AFF2 (NM_001169122) Human Tagged ORF Clone
CAT＃: RG230691
- TrueORF®
AFF2 (tGFP-tagged) - Human AF4/FMR2 family, member 2 (AFF2), transcript variant 2
ORF Plasmid: DDK
"NM_001169122" in other vectors (4)
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CNY 13,970.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230691 representing NM_001169122
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTATTCGACTTTTTCAGAGACTGGGACTTGGAGCAGCAGTGTCACTATGAACAAGACCGTAGTG CACTTAAAAAAAGGGAATGGGAGCGGAGGAATCAAGAAGTCCAGCAAGAAGACGATCTCTTTTCTTCAGG CTTTGATCTTTTTGGGGAGCCATACAAGACAAACAAAGGTGATGCACTTGCCAACCGAGTCCAGAACACG CTTGGAAACTATGATGAAATGAAGAATTTGCTAACTAACCATTCTAATCAGAATCACCTAGTGGGAATTC CAAAGAATTCTGTGCCCCAGAATCCCAACAACAAAAATGAACCAAGCTTTTTTCCAGAACAAAAGAACAG AATAATTCCACCTCACCAGGATAATACCCATCCTTCAGCACCAATGCCTCCACCTTCTGTTGTGATACTG AATTCAACTCTAATACACAGCAACAGAAAATCAAAACCTGAGTGGTCACGTGATAGTCATAACCCTAGCA CTGTACTGGCAAGCCAGGCCAGTGGTCAGCCAAACAAGATGCAGACTTTGACACAGGACCAGTCTCAAGC CAAACTGGAAGACTTCTTTGTCTACCCAGCTGAACAGCCCCAGATTGGAGAAGTTGAAGAGTCAAACCCA TCTGCAAAGGAAGACAGTAACCCTAATTCTAGTGGAGAAGATGCTTTCAAAGAAATCTTTCAATCCAATT CACCGGAAGAATCTGAATTCGCCGTGCAAGCGCCTGGGTCTCCCCTAGTGGCTTCCTCTTTATTAGCTCC TAGCAGTGGCCTTTCAGTTCAAAACTTCCCACCAGGGCTTTACTGCAAAACAAGCATGGGGCAGCAAAAG CCAACTGCATACGTCAGACCCATGGATGGCCAGGACCAGGCACCGGACATCTCACCAACACTGAAACCTT CAATTGAATTTGAGAACAGCTTTGGGAATCTGTCATTTGGAACACTCTTGGATGGAAAACCCAGTGCAGC CAGTTCAAAGACTAAACTGCCAAAGTTCACCATCCTCCAAACAAGTGAAGTAAGCCTTCCCAGTGATCCA AGCTGTGTTGAAGAAATCTTGCGGGAATCGCAGCATCTGACCCCAGGATTCACCTTACAAAAGTGGAATG ACCCAACCACCAGAGCTTCTACAAAGTCAGTGTCTTTCAAATCGATGCTTGAGGATGACCTGAAGCTGAG CAGTGATGAAGATGACCTTGAGCCTGTGAAGACCTTGACCACTCAGTGCACTGCCACTGAGCTCTACCAG GCTGTTGAAAAGGCAAAACCTAGGAATAATCCTGTGAACCCACCCTTGGCCACTCCCCAGCCCCCACCTG CAGTGCAAGCCAGCGGGGGTTCTGGCAGCTCCAGCGAATCGGAGAGCAGCTCTGAGTCGGATTCAGACAC TGAAAGTAGCACCACTGACAGCGAATCTAATGAGGCACCTCGTGTGGCAACTCCAGAGCCTGAGCCACCC TCAACCAACAAGTGGCAACTGGATAAATGGCTTAACAAAGTGACATCCCAGAACAAGTCTTTTATTTGTG GCCAAAATGAAACACCCATGGAGACTATTTCTCTGCCTCCTCCAATCATCCAACCAATGGAAGTCCAGAT GAAAGTGAAGACGAATGCCAGTCAGGTCCCAGCTGAACCCAAAGAAAGGCCTCTCCTCAGTCTCATTAGG GAGAAAGCCCGTCCACGGCCCACTCAGAAAATTCCAGAAACAAAGGCTTTGAAGCATAAGTTGTCAACAA CTAGTGAGACAGTGTCTCAAAGGACAATTGGGAAAAAACAGCCCAAAAAAGTTGAGAAGAACACCAGCAC TGACGAGTTTACCTGGCCCAAACCAAATATTACCAGCAGCACTCCCAAAGAAAAAGAAAGTGTGGAGCTT CATGACCCACCAAGAGGCCGCAACAAAGCCACTGCCCACAAACCAGCCCCTAGGAAAGAACCAAGACCTA ACATCCCTTTGGCTCCCGAGAAGAAGAAGTACAGAGGGCCTGGCAAGATTGTGCCAAAGTCTCGGGAATT CATTGAAACAGATTCATCTACATCTGACTCCAACACAGATCAGGAAGAGACCCTGCAAATCAAAGTCCTG CCTCCGTGCATTATTTCTGGAGGTAATACTGCCAAATCCAAGGAAATCTGTGGTGCCAGCCTGACCCTCA GCACCTTAATGAGTAGCAGTGGCAGCAACAACAACTTATCCATCAGTAATGAAGAGCCAACATTTTCACC TATTCCTGTCATGCAAACTGAAATCCTGTCCCCTCTGCGAGATCATGAGAACCTGAAAAACCTCTGGGTG AAGATTGACCTTGACTTACTCTCTAGAGTACCTGGCCACAGCTCACTCCATGCAGCACCTGCCAAGCCAG ACCACAAGGAGACTGCCACAAAACCCAAGCGTCAGACAGCTGTCACAGCTGTGGAGAAACCAGCCCCTAA GGGCAAACGTAAGCACAAGCCAATAGAAGTTGCAGAGAAGATCCCTGAGAAGAAGCAGCGCCTGGAGGAG GCCACAACTATCTGCTTGCTCCCTCCTTGCATCTCACCAGCCCCACCCCACAAGCCTCCCAACACTAGAG AAAATAATTCATCCAGGAGAGCAAATAGAAGAAAGGAAGAAAAACTATTTCCTCCTCCACTTTCCCCACT GCCAGAGGACCCTCCACGCCGCAGAAATGTCAGTGGCAATAATGGTCCCTTTGGTCAAGACAAAAACATC GCCATGACTGGACAAATCACATCTACCAAACCTAAGAGAACTGAAGGCAAATTCTGTGCTACTTTCAAAG GGATATCGGAGGGAGACACTCCAAAAAAGGCATCCTCTGCCACCATCACTGTCACCAATACTGCTATTGC CACTGCTACTGTCACTGCTACTGCCATTGTCACCACCACTGTCACAGCTACTGCCACCGCCACGGCCACC ACCACAACTACTACCACTACCATTTCCACCATCACCTCTACCATCACTACTGGCCTCATGGATAGCAGTC ACCTGGAGATGACGTCCTGGGCGGCTCTGCCCCTTCTATCCAGCAGCAGCACTAATGTCCGGAGACCCAA GCTCACTTTTGATGACTCGGTTCACAATGCTGATTATTACATGCAAGAAGCTAAGAAGCTGAAGCACAAA GCTGATGCACTGTTCGAGAAATTTGGCAAAGCTGTGAATTATGCTGATGCCGCCCTCTCCTTCACTGAAT GTGGCAATGCCATGGAACGCGACCCTCTGGAAGCAAAGTCCCCATACACCATGTACTCTGAGACTGTGGA GCTCCTCAGGTATGCAATGAGGCTGAAGAACTTTGCAAGTCCCTTGGCTTCGGATGGGGACAAAAAGCTA GCAGTACTATGCTACCGATGTTTATCACTCCTCTATTTGAGAATGTTTAAGCTGAAGAAGGACCATGCTA TGAAGTACTCCAGATCACTGATGGAATATTTTAAGCAAAATGCTTCAAAAGTCGCACAGATACCCTCTCC ATGGGTAAGCAATGGAAAGAACACTCCATCCCCAGTGTCTCTCAACAACGTCTCCCCCATCAACGCAATG GGGAACTGTAACAATGGCCCAGTCACCATTCCCCAGCGCATTCACCACATGGCTGCCAGCCACGTCAACA TCACTAGCAATGTGTTACGGGGCTATGAACACTGGGATATGGCCGACAAACTGACAAGAGAAAACAAAGA ATTCTTTGGTGATCTGGACACGCTGATGGGGCCTCTGACCCAGCACAGCAGCATGACCAATCTTGTCCGC TACGTTCGCCAAGGACTGTGTTGGCTGCGCATCGATGCCCACTTGTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230691 representing NM_001169122
Red=Cloning site Green=Tags(s) MDLFDFFRDWDLEQQCHYEQDRSALKKREWERRNQEVQQEDDLFSSGFDLFGEPYKTNKGDALANRVQNT LGNYDEMKNLLTNHSNQNHLVGIPKNSVPQNPNNKNEPSFFPEQKNRIIPPHQDNTHPSAPMPPPSVVIL NSTLIHSNRKSKPEWSRDSHNPSTVLASQASGQPNKMQTLTQDQSQAKLEDFFVYPAEQPQIGEVEESNP SAKEDSNPNSSGEDAFKEIFQSNSPEESEFAVQAPGSPLVASSLLAPSSGLSVQNFPPGLYCKTSMGQQK PTAYVRPMDGQDQAPDISPTLKPSIEFENSFGNLSFGTLLDGKPSAASSKTKLPKFTILQTSEVSLPSDP SCVEEILRESQHLTPGFTLQKWNDPTTRASTKSVSFKSMLEDDLKLSSDEDDLEPVKTLTTQCTATELYQ AVEKAKPRNNPVNPPLATPQPPPAVQASGGSGSSSESESSSESDSDTESSTTDSESNEAPRVATPEPEPP STNKWQLDKWLNKVTSQNKSFICGQNETPMETISLPPPIIQPMEVQMKVKTNASQVPAEPKERPLLSLIR EKARPRPTQKIPETKALKHKLSTTSETVSQRTIGKKQPKKVEKNTSTDEFTWPKPNITSSTPKEKESVEL HDPPRGRNKATAHKPAPRKEPRPNIPLAPEKKKYRGPGKIVPKSREFIETDSSTSDSNTDQEETLQIKVL PPCIISGGNTAKSKEICGASLTLSTLMSSSGSNNNLSISNEEPTFSPIPVMQTEILSPLRDHENLKNLWV KIDLDLLSRVPGHSSLHAAPAKPDHKETATKPKRQTAVTAVEKPAPKGKRKHKPIEVAEKIPEKKQRLEE ATTICLLPPCISPAPPHKPPNTRENNSSRRANRRKEEKLFPPPLSPLPEDPPRRRNVSGNNGPFGQDKNI AMTGQITSTKPKRTEGKFCATFKGISEGDTPKKASSATITVTNTAIATATVTATAIVTTTVTATATATAT TTTTTTTISTITSTITTGLMDSSHLEMTSWAALPLLSSSSTNVRRPKLTFDDSVHNADYYMQEAKKLKHK ADALFEKFGKAVNYADAALSFTECGNAMERDPLEAKSPYTMYSETVELLRYAMRLKNFASPLASDGDKKL AVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKQNASKVAQIPSPWVSNGKNTPSPVSLNNVSPINAM GNCNNGPVTIPQRIHHMAASHVNITSNVLRGYEHWDMADKLTRENKEFFGDLDTLMGPLTQHSSMTNLVR YVRQGLCWLRIDAHLL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001169122 |
| ORF Size | 3828 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001169122.2 |
| RefSeq Size | 13641 bp |
| RefSeq ORF | 3831 bp |
| Locus ID | 2334 |
| UniProt ID | P51816 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a putative transcriptional activator that is a member of the AF4\FMR2 gene family. This gene is associated with the folate-sensitive fragile X E locus on chromosome X. A repeat polymorphism in the fragile X E locus results in silencing of this gene causing Fragile X E syndrome. Fragile X E syndrome is a form of nonsyndromic X-linked cognitive disability. In addition, this gene contains 6-25 GCC repeats that are expanded to >200 repeats in the disease state. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230691 | AFF2 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 2 |
CNY 12,730.00 |
|
| RC230691L3 | Lenti-ORF clone of AFF2 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 2 |
CNY 14,630.00 |
|
| RC230691L4 | Lenti-ORF clone of AFF2 (mGFP-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 2 |
CNY 14,630.00 |
|
| SC329329 | AFF2 (untagged)-Human AF4/FMR2 family member 2 (AFF2) transcript variant 2 |
CNY 24,700.00 |
