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FREM1 (NM_001177704) Human Tagged ORF Clone
CAT＃: RG230453
- TrueORF®
FREM1 (tGFP-tagged) - Human FRAS1 related extracellular matrix 1 (FREM1), transcript variant 2
ORF Plasmid: DDK
"NM_001177704" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BNAR; C9orf143; C9orf145; C9orf154; MOTA; TILRR; TRIGNO2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230453 representing NM_001177704
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGACACAAGAATCCATGCTGAAGGCTGCCTTGCCTCTCTTTACCAGATTCATCATCAGCAATGGAC TGCGGACCGAGCACGGGGTGTTTGAGATCACACTGGAGACTGTGGACAGAGCCCTGCCTGTGGTAACCAG GAACAAGGGGTTGAGACTGGCCCAAGGGGCCGTGGGCCTGCTTTCCCCTGACCTCCTTCAGCTGACCGAC CCTGATACACCTGCGGAGAACCTCACCTTCCTCTTGGTTCAGCTCCCCCAGCATGGCCAGCTCTACCTGT GGGGGACAGGGCTACTTCAACACAATTTCACCCAGCAGGATGTGGACAGCAAGAATGTGGCCTATCGGCA CTCAGGAGGGGACTCCCAGACTGACTGCTTTACTTTCATGGCCACAGATGGGACAAACCAAGGCTTTATT GTGAATGGGAGAGTGTGGGAAGAACCTGTTTTATTCACCATTCAGGTAGACCAATTGGACAAAACAGCTC CTCGTATCACACTCTTGCATTCCCCTTCTCAAGTGGGGCTCCTGAAAAATGGCTGCTACGGGATTTACAT CACTTCCCGCGTGTTGAAGGCATCAGACCCTGACACTGAGGACGATCAGATCATCTTTAAAATTCTACAA GGCCCAAAACATGGACATCTGGAGAACACAACAACAGGTGAATTTATCCATGAGAAATTTAGCCAAAAGG ACTTAAACAGTAAGACTATTCTTTACATCATAAACCCATCTTTGGAAGTAAATTCAGATACCGTGGAATT TCAAATCATGGACCCCACAGGGAACTCGGCCACTCCTCAAATTTTGGAACTGAAGTGGTCTCATATTGAA TGGTCACAGACCGAATATGAAGTCTGTGAGAATGTGGGTTTGTTGCCCTTGGAAATTATCAGAAGGGGAT ATTCCATGGACTCGGCCTTTGTGGGTATAAAGGTCAACCAAGTGTCAGCTGCAGTTGGAAAAGATTTCAC CGTGATTCCATCTAAACTGATTCAGTTTGACCCAGGAATGTCAACTAAGATGTGGAATATAGCAATTACC TATGACGGATTAGAGGAAGATGATGAGGTCTTTGAAGTAATTCTGAACTCCCCTGTGAATGCAGTTCTTG GCACAAAGACAAAAGCTGCAGTGAAAATTTTGGACTCAAAAGGAGGACAATGCCATCCTTCATATTCCTC CAACCAAAGCAAGCACAGCACATGGGAGAAGGGCATTTGGCATCTGCTGCCCCCAGGGTCTTCCTCATCC ACCACTTCTGGTTCCTTTCATCTGGAAAGAAGACCTCTTCCATCTTCCATGCAGCTAGCAGTCATCAGGG GAGACACCCTGCGGGGCTTTGATTCTACAGATCTTTCTCAAAGGAAGCTTAGGACCCGTGGGAATGGCAA AACAGTTCGTCCATCCTCTGTTTATAGAAATGGAACAGACATCATCTATAATTATCATGGGATAGTTTCC TTGAAACTGGAGGATGACAGTTTCCCAACTCACAAAAGGAAGGCCAAAGTATCCATCATTAGTCAGCCAC AAAAGACAATCAAAGTGGCAGAACTGCCTCAAGCAGATAAGGTGGAATCCACAACTGACTCACACTTCCC CAGACAGGACCAGTTGCCCTCATTTCCAAAGAACTGCACTCTGGAATTAAAGGGACTCTTCCATTTTGAA GAAGGCATCCAGAAGCTGTATCAGTGCAATGGGATCGCCTGGAAAGCCTGGAGTCCCCAAACCAAGGATG TGGAAGACAAATCCTGTCCAGCCGGGTGGCACCAGCACTCAGGCTACTGTCACATCTTGATCACAGAGCA GAAAGGCACCTGGAATGCGGCTGCCCAAGCTTGCAGGGAACAATACCTGGGCAACCTTGTAACTGTATTC TCCAGGCAGCACATGCGGTGGCTCTGGGACATTGGTGGGAGAAAGTCCTTTTGGATAGGTTTGAACGACC AAGTGCATGCTGGCCACTGGGAGTGGATCGGTGGTGAACCTGTTGCCTTCACCAATGGGAGAAGAGGGCC CTCTCAACGCTCCAAGCTTGGAAAGAGCTGTGTTTTGGTTCAAAGACAAGGGAAATGGCAAACAAAAGAC TGTAGGAGAGCCAAACCTCATAATTATGTGTGTTCCAGAAAACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230453 representing NM_001177704
Red=Cloning site Green=Tags(s) MVTQESMLKAALPLFTRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTD PDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFI VNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQ GPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIE WSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAIT YDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSS TTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVS LKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFE EGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVF SRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKD CRRAKPHNYVCSRKL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001177704 |
| ORF Size | 2145 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001177704.3 |
| RefSeq Size | 4967 bp |
| RefSeq ORF | 2148 bp |
| Locus ID | 158326 |
| UniProt ID | Q5H8C1 |
| Protein Families | Protease, Transmembrane |
| Gene Summary | This gene encodes a basement membrane protein that may play a role in craniofacial and renal development. Mutations in this gene have been associated with bifid nose with or without anorectal and renal anomalies. Alternatively spliced transcript variants encoding different isoforms have been described. PubMed ID 19940113 describes one such variant that initiates transcription within a distinct, internal exon; the resulting shorter isoform (named Toll-like/interleukin-1 receptor regulator, TILRR) is suggested to be a co-receptor of the interleukin 1 receptor family and may regulate receptor function and Toll-like receptor/interleukin 1 receptor signal transduction, contributing to the control of inflammatory response activation. [provided by RefSeq, Apr 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230453 | FREM1 (Myc-DDK-tagged)-Human FRAS1 related extracellular matrix 1 (FREM1), transcript variant 2 |
CNY 5,800.00 |
|
| RC230453L3 | Lenti-ORF clone of FREM1 (Myc-DDK-tagged)-Human FRAS1 related extracellular matrix 1 (FREM1), transcript variant 2 |
CNY 7,700.00 |
|
| RC230453L4 | Lenti-ORF clone of FREM1 (mGFP-tagged)-Human FRAS1 related extracellular matrix 1 (FREM1), transcript variant 2 |
CNY 7,700.00 |
|
| SC329091 | FREM1 (untagged)-Human FRAS1 related extracellular matrix 1 (FREM1) transcript variant 2 |
CNY 13,780.00 |
