PLAAT5 (NM_001146728) Human Tagged ORF Clone
CAT#: RG228184
- TrueORF®
HRASLS5 (tGFP-tagged) - Human HRAS-like suppressor family, member 5 (HRASLS5), transcript variant 3
ORF Plasmid: DDK
"NM_001146728" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | HRASLS5; HRLP5; HRSL5; iNAT; PLAAT-5; RLP1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG228184 representing NM_001146728
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCTGAGCCCGGGCGCCGAGGGGGAGTACGCGCTCCGCCTCCCTAGGATTCCCCCACCCCTCCCCA AACCCGCCTCGCGAACCGCCGGTACCGGGCCCAAGGACCAGCCGCCTGCGCTCAGACGTTCAGCTGTGCC CCACTCAGGTCTAAATAGCATTTCCCCCCTTGAATTAGAAGAATCCGTGGGATTCGCAGCGTTGGTCCAG CTCCCAGCCAAGCAGCCTCCGCCGGGCACATTAGAACAGGGCAGAAGCATCCAGCAAGGGGAGAAGGCTG TAGTTAGCTTGGAGACCACACCCAGCCAGAAAGCAGACTGGAGTTCAATTCCAAAGCCTGAGAATGAAGG CAAGTTAATAAAGCAAGCAGCTGAGGGAAAACCAAGACCCAGACCTGGAGACCTGATTGAGATTTTTCGA ATTGGCTATGAGCACTGGGCCATCTATGTAGAAGATGATTGCGTGGTCCATCTGGCTCCCCCAAGTGAGG AGTTTGAGGTGGGCAGCATTACTTCCATCTTTAGCAATCGGGCCGTGGTGAAATACAGTCGTCTGGAGGA TGTGCTGCATGGCTGCTCCTGGAAGGTCAATAACAAGCTAGATGGGACGTACCTGCCCTTGCCGGTGGAC AAGATCATCCAGCGTACAAAAAAGATGGTCAACAAGATCGTGCAGTACAGCCTGATTGAAGGGAACTGTA GAGCACGCCCTGATGGAAGGAGCGAAGGCTGCTGGAGCAGTTATTTCAGCTGTAGTGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228184 representing NM_001146728
Red=Cloning site Green=Tags(s) MGLSPGAEGEYALRLPRIPPPLPKPASRTAGTGPKDQPPALRRSAVPHSGLNSISPLELEESVGFAALVQ LPAKQPPPGTLEQGRSIQQGEKAVVSLETTPSQKADWSSIPKPENEGKLIKQAAEGKPRPRPGDLIEIFR IGYEHWAIYVEDDCVVHLAPPSEEFEVGSITSIFSNRAVVKYSRLEDVLHGCSWKVNNKLDGTYLPLPVD KIIQRTKKMVNKIVQYSLIEGNCRARPDGRSEGCWSSYFSCSG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001146728 |
ORF Size | 759 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001146728.1, NP_001140200.1 |
RefSeq Size | 3077 bp |
RefSeq ORF | 762 bp |
Locus ID | 117245 |
Gene Summary | Exhibits both phospholipase A1/2 and acyltransferase activities (PubMed:22825852, PubMed:26503625). Shows phospholipase A1 (PLA1) and A2 (PLA2) activity, catalyzing the calcium-independent release of fatty acids from the sn-1 or sn-2 position of glycerophospholipids (PubMed:22825852). Shows N-acyltransferase activity, catalyzing the calcium-independent transfer of a fatty acyl group at the sn-1 position of phosphatidylcholine (PC) and other glycerophospholipids to the primary amine of phosphatidylethanolamine (PE), forming N-acylphosphatidylethanolamine (NAPE), which serves as precursor for N-acylethanolamines (NAEs) (PubMed:19000777, PubMed:22825852).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC228184 | HRASLS5 (Myc-DDK-tagged)-Human HRAS-like suppressor family, member 5 (HRASLS5), transcript variant 3 |
CNY 2,400.00 |
|
RC228184L3 | Lenti ORF clone of Human HRAS-like suppressor family, member 5 (HRASLS5), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC228184L4 | Lenti ORF clone of Human HRAS-like suppressor family, member 5 (HRASLS5), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
SC326819 | HRASLS5 (untagged)-Human HRAS-like suppressor family member 5 (HRASLS5) transcript variant 3 |
CNY 3,990.00 |