SPHKAP (NM_001142644) Human Tagged ORF Clone
CAT#: RG227959
- TrueORF®
SPHKAP (tGFP-tagged) - Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1
ORF Plasmid: DDK
"NM_001142644" in other vectors (4)
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CNY 15,200.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | SKIP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227959 representing NM_001142644
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGCAACTCCCTGCTCTCGGTACCAAGCAACTTGGAGTCATCACGGATGTATGACGTTTTGGAAC CGCAGCAGGGCAGAGGCTGTGGCAGCTCAGGAAGCGGCCCGGGGAACTCCATCACAGCCTGTAAGAAGGT TCTTCGCAGCAATAGCCTGCTGGAGTCAACAGACTACTGGTTGCAGAATCAGAGGATGCCCTGCCAAATT GGTTTTGTAGAAGACAAGTCTGAAAACTGTGCTTCTGTCTGCTTTGTGAATCTTGATGTGAACAAGGATG AATGCAGCACAGAGCACCTGCAACAGAAACTGGTCAACGTTTCACCAGATCTTCCAAAACTTATCAGTTC CATGAATGTCCAACAACCAAAAGAAAATGAAATTGTTGTCCTAAGTGGGTTAGCCTCTGGAAATCTCCAG GCAGATTTTGAAGTTTCACAGTGCCCTTGGCTGCCAGATATCTGCTTGGTCCAATGTGCAAGAGGGAACA GACCAAACAGTACCAACTGCATCATCTTTGAAATCAACAAATTTCTGATTGGTCTGGAACTGGTGCAGGA GCGACAGCTCCACCTGGAAACAAACATCTTGAAACTGGAGGATGACACGAACTGTTCCTTATCTTCAATC GAGGAAGACTTTCTCACCGCTTCTGAGCACTTGGAGGAGGAAAGCGAGGTGGATGAATCTAGGAACGATT ATGAAAATATAAATGTCTCAGCCAATGTTTTGGAAAGTAAACAGCTAAAGGGAGCCACCCAGGTGGAATG GAATTGCAACAAGGAAAAGTGGCTTTATGCTTTGGAAGACAAATACATCAACAAATATCCCACACCATTG ATTAAAACAGAACGATCTCCAGAAAACCTAACAAAGAACACAGCCTTGCAGAGTCTAGATCCCTCAGCCA AGCCATCACAGTGGAAAAGAGAAGCTGTGGGGAATGGGAGACAAGCCACACATTATTATCATTCAGAAGC TTTTAAAGGTCAAATGGAAAAATCACAGGCACTGTATATTCCAAAAGATGCTTATTTCTCCATGATGGAT AAAGATGTACCTTCTGCATGTGCTGTGGCAGAGCAGAGAAGCAACCTAAACCCAGGAGACCATGAAGACA CAAGAAACGCTCTCCCTCCTAGACAAGATGGAGAAGTCACCACTGGCAAGTATGCTACAAATTTAGCAGA ATCCGTGCTGCAGGATGCATTTATTAGATTATCTCAATCTCAGTCCACATTACCCCAGGAATCTGCAGTC AGTGTTTCTGTAGGAAGTTCTCTGCTTCCCAGTTGCTATTCCACAAAAGATACAGTGGTTTCTCGGTCAT GGAATGAGCTCCCCAAAATCGTCGTTGTTCAGAGTCCAGATGGCAGTGATGCTGCCCCACAGCCAGGCAT CTCCTCCTGGCCTGAGATGGAAGTCTCTGTTGAAACCTCAAGCATCCTCTCTGGAGAGAACTCCAGCAGA CAACCCCAGAGTGCTCTAGAAGTGGCGTTAGCTTGTGCAGCCACTGTGATTGGAACTATTTCCAGTCCAC AGGCCACAGAAAGACTCAAAATGGAGCAAGTGGTCTCGAACTTTCCCCCAGGGAGCAGTGGTGCACTGCA AACTCAAGCACCCCAAGGACTCAAGGAACCTTCCATCAATGAGTACTCCTTTCCATCTGCTTTGTGTGGC ATGACTCAGGTGGCCAGTGCCGTGGCTGTCTGTGGTCTGGGTGAAAGAGAAGAGGTGACATGCTCAGTGG CTCCAAGTGGTAGCCTCCCGCCTGCAGCTGAGGCTTCTGAAGCCATGCCCCCACTTTGTGGTTTAGCAAG CATGGAGCTTGGCAAGGAAGCCATTGCCAAGGGATTGCTCAAGGAGGCTGCTCTGGTTTTAACAAGGCCT AATACCTACAGCAGCATTGGAGACTTTCTGGACTCCATGAACAGGAGAATCATGGAAACTGCTTCAAAGT CTCAGACCCTGTGCTCAGAAAATGTCGTCAGGAATGAACTGGCACATACCCTGTCCAATGTTATCCTGAG GCATTCCATTGATGAAGTTCACCACAAAAATATGATAATCGACCCCAATGACAACAGGCATTCATCTGAA ATTCTGGACACCTTAATGGAAAGTACAAATCAACTGCTTTTAGATGTGATATGCTTCACGTTCAAGAAGA TGAGTCATATTGTACGGCTTGGTGAATGTCCTGCTGTCCTTTCTAAGGAGACCATCAGAAGGAGGGAGAC AGAACCAAGCTGCCAGCCATCTGATCCGGGTGCTAGTCAAGCTTGGACAAAAGCCACTGAATCCTCCAGC AGCTCTCCACTTAGCAATTCACACAACACGAGTCTTGTCATCAACAATCTTGTGGATGGCATGTATTCAA AACAAGACAAGGGTGGAGTGAGGCCAGGCCTCTTCAAGAACCCCACGCTGCAGTCACAATTATCACGTAG TCACAGAGTGCCCGATTCTTCAACTGCTACAACATCCTCCAAGGAAATATATCTGAAAGGAATAGCAGGA GAGGATACAAAAAGCCCTCATCACAGTGAGAATGAATGCAGAGCCTCTTCCGAAGGACAAAGGTCCCCAA CGGTCAGCCAGTCCAGAAGTGGTTCCCAGGAGGCTGAGGAGAGTATCCACCCAAACACCCAAGAAAAGTA CAACTGTGCCACATCTCGCATCAACGAAGTTCAAGTCAACCTGTCCTTGTTAGGGGATGACCTGCTGCTT CCTGCTCAATCCACGCTTCAAACAAAGCATCCAGACATCTACTGCATTACAGACTTTGCGGAAGAATTAG CAGACACGGTCGTCTCCATGGCAACTGAAATTGCAGCGATTTGCCTTGACAACTCCAGTGGAAAACAACC CTGGTTTTGTGCATGGAAAAGAGGGAGTGAGTTTCTGATGACACCCAACGTACCCTGCCGATCCTTGAAG AGGAAGAAAGAGAGCCAGGGGAGCGGGACCGCTGTGAGGAAACACAAGCCTCCCCGGCTCAGTGAGATCA AGAGGAAGACGGACGAGCACCCTGAGCTTAAAGAAAAGCTGATGAACAGGGTTGTGGATGAGTCCATGAA CCTTGAAGATGTCCCAGATTCTGTCAATCTTTTTGCCAATGAAGTGGCAGCCAAGATCATGAACCTAACG GAGTTCTCTATGGTGGACGGCATGTGGCAGGCGCAGGGCTATCCCCGGAATCGGTTACTGAGTGGCGACA GGTGGAGCCGGCTGAAGGCCTCCAGCTGCGAAAGCATTCCTGAGGAAGACTCCGAGGCCAGGGCCTATGT CAACAGCCTGGGCTTAATGAGCACGCTGAGCCAGCCGGTCAGCAGGGCCAGCTCTGTCTCCAAGCAGTCG AGCTGTGAGAGCATCACCGATGAGTTTTCCAGGTTCATGGTGAACCAGATGGAAAATGAAGGGAGAGGAT TTGAGTTACTGCTGGATTACTATGCTGGCAAGAACGCCAGCAGCATTCTGAACTCAGCCATGCAACAGGC GTGCCGGAAAAGTGACCACCTCAGTGTGAGGCCTAGCTGTCCCTCTAAGCAGTCCAGCACAGAGAGCATC ACTGAGGAGTTCTACAGGTACATGCTGAGGGACATCGAAAGAGACAGCAGAGAAAGTGCCTCCTCCAGAC GGAGCAGCCAGGATTGGACAGCCGGCCTGCTGTCTCCTTCTCTGCGATCCCCAGTGTGCCACAGACAGTC GTCCATGCCAGACAGCAGATCCCCATGCTCCAGGCTGACAGTGAATGTGCCCATCAAAGCCAACTCTTTA GATGGCTTTGCTCAGAACTGCCCACAAGATTTCCTAAGCGTGCAGCCGGTCAGTAGCGCGTCCTCATCCG GTCTCTGCAAATCTGACTCTTGCTTGTATCGGAGAGGTGGGACTGACCACATCACCAACATGTTAATTCA TGAAACGTGGGCTAGCTCCATTGAGGCTCTCATGCGCAAGAACAAAATCATTGTGGATGATGCAGAGGAA GCTGACACTGAGCCTGTTTCTGGTGGCTCTCCCTCGCAAGCAGAGAAGTGTGCAAATAGATTAGCTGCGA GCAGGATGTGCAGTGGGCCAACTCTGCTTGTTCAGGAGTCTCTCGATTGCCCGAGGAAAGACTCTGTTAC CGAATGTAAACAGCCCCCAGTGTCATCTTTGAGCAAAACTGCTTCTCTTACAAACCACAGCCCTTTAGAT TCTAAAAAAGAAACTTCCTCGTGCCAGGACCCTGTACCAATAAACCACAAAAGGCGATCACTTTGCTCGA GGGAAGTGCCTTTGATTCAGATTGAAACAGATCAGAGAGAAGCCTGTGCTGGGGAACCTGAACCCTTCCT TTCCAAAAGCAGCCTCCTAGAGGAAGCAGAAGGGCATTCGAATGACAAAAACATCCCAGATGTGGTGAGA GGTGGAGACACAGCCGTGAGCGCTTGTCAAATCCATAGTGACAGCCTTGATACCAGAGATGTACCAGAGG CTGAAGCCTCCACAGAAGCCAGAGCCCCCGATGAGGCCCCCAACCCTCCAAGCAGCAGCGAGGAGAGCAC AGGCAGCTGGACCCAGCTTGCCAATGAGGAAGACAACCCAGATGACACAAGTAGCTTTCTCCAGCTCAGT GAGCGATCCATGAGCAATGGCAACAGTAGTGCCACTAGCAGTCTTGGCATTATGGATCTGGACATTTATC AGGAAAGCATGCCATCTTCTCCCATGATTAATGAATTAGTAGAAGAAAAGAAGATTCTTAAAGGACAGTC AGAAAGCACAGAGGCACCTGCATCTGGACCGCCTACGGGAACAGCCAGCCCCCAGAGGAGCCTGCTGGTG ATCAACTTTGACCTGGAGCCAGAGTGTCCAGATGCCGAGCTCCGAGCCACTCTGCAGTGGATAGCTGCCT CTGAACTGGGGATTCCCACCATCTACTTTAAGAAATCTCAGGAAAACAGAATTGAAAAGTTTCTAGATGT CGTGCAGCTGGTTCATCGGAAGTCCTGGAAAGTGGGTGATATCTTCCATGCAGTTGTCCAGTACTGCAAA ATGCATGAGGAGCAGAAGGATGGGAGACTGAGTCTCTTTGACTGGCTCTTGGAACTGGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227959 representing NM_001142644
Red=Cloning site Green=Tags(s) MDGNSLLSVPSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYWLQNQRMPCQI GFVEDKSENCASVCFVNLDVNKDECSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQ ADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSI EEDFLTASEHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPL IKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMD KDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAV SVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETSSILSGENSSR QPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCG MTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAALVLTRP NTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSE ILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSS SSPLSNSHNTSLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAG EDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLL PAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLK RKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNLFANEVAAKIMNLT EFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQS SCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESI TEEFYRYMLRDIERDSRESASSRRSSQDWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSL DGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRKNKIIVDDAEE ADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECKQPPVSSLSKTASLTNHSPLD SKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVR GGDTAVSACQIHSDSLDTRDVPEAEASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLS ERSMSNGNSSATSSLGIMDLDIYQESMPSSPMINELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLV INFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCK MHEEQKDGRLSLFDWLLELG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001142644 |
ORF Size | 5100 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001142644.2 |
RefSeq Size | 6913 bp |
RefSeq ORF | 5103 bp |
Locus ID | 80309 |
UniProt ID | Q2M3C7 |
Protein Families | Druggable Genome |
Gene Summary | Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227959 | SPHKAP (Myc-DDK-tagged)-Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1 |
CNY 18,280.00 |
|
RC227959L1 | Lenti ORF clone of Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1, Myc-DDK-tagged |
CNY 20,680.00 |
|
RC227959L3 | Lenti ORF clone of Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1, Myc-DDK-tagged |
CNY 15,680.00 |
|
SC325420 | SPHKAP (untagged)-Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 1 |
CNY 26,790.00 |