WDR79 (WRAP53) (NM_001143992) Human Tagged ORF Clone
CAT#: RG227786
- TrueORF®
WRAP53 (tGFP-tagged) - Human WD repeat containing, antisense to TP53 (WRAP53), transcript variant 4
ORF Plasmid: DDK
"NM_001143992" in other vectors (4)
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CNY 5,040.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DKCB3; TCAB1; WDR79 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227786 representing NM_001143992
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACTTTGGAGACTCAACCGTTAGCTCCGGACTGCTGTCCTTCAGACCAGGACCCAGCTCCAGCCC ATCCTTCTCCCCACGCTTCCCCGATGAATAAAAATGCGGACTCTGAACTGATGCCACCGCCTCCCGAAAG GGGGGATCCGCCCCGGTTGTCCCCAGATCCTGTGGCTGGCTCAGCTGTGTCCCAGGAGCTACGGGAGGGG GACCCAGTTTCTCTCTCCACTCCCCTGGAAACAGAGTTTGGTTCCCCTAGTGAGTTGAGTCCTCGAATCG AGGAGCAAGAACTTTCTGAAAATACAAGCCTTCCTGCAGAAGAAGCAAACGGGAGCCTTTCTGAAGAAGA AGCGAACGGGCCAGAGTTGGGGTCTGGAAAAGCCATGGAAGATACCTCTGGGGAACCCGCTGCAGAGGAC GAGGGAGACACCGCTTGGAACTACAGCTTCTCCCAGCTGCCTCGATTTCTCAGTGGTTCCTGGTCAGAGT TCAGCACCCAACCTGAGAACTTCTTGAAAGGCTGTAAGTGGGCTCCTGACGGTTCCTGCATCTTGACCAA TAGTGCTGATAACATCTTGCGAATTTATAACCTGCCCCCAGAGCTGTACCATGAGGGGGAGCAGGTGGAA TATGCAGAAATGGTCCCTGTCCTTCGAATGGTGGAAGGTGATACCATCTATGATTACTGCTGGTATTCTC TGATGTCCTCAGCCCAGCCAGACACCTCCTACGTGGCCAGCAGCAGCCGGGAGAACCCGATTCATATCTG GGACGCATTCACTGGAGAGCTCCGGGCTTCCTTTCGCGCCTACAACCACCTGGATGAGCTGACGGCAGCC CATTCGCTCTGCTTCTCCCCGGATGGCTCCCAGCTCTTCTGTGGCTTCAACCGGACTGTGCGTGTTTTTT CCACGGCCCGGCCTGGCCGAGACTGCGAGGTCCGAGCCACATTTGCAAAAAAGCAGGGCCAGAGCGGCAT CATCTCCTGCATAGCCTTCAGCCCAGCCCAGCCCCTCTATGCCTGTGGCTCCTACGGCCGCTCCCTGGGT CTGTATGCCTGGGATGATGGCTCCCCTCTCGCCTTGCTGGGAGGGCACCAAGGGGGCATCACCCACCTCT GCTTTCATCCCGATGGCAACCGCTTCTTCTCAGGAGCCCGCAAGGATGCTGAGCTCCTGTGCTGGGATCT CCGGCAGTCTGGTTACCCACTGTGGTCCCTGGGTCGAGAGGTGACCACCAATCAGCGCATCTACTTCGAT CTGGACCCGACCGGGCAGTTCCTAGTGAGTGGCAGCACGAGCGGGGCTGTCTCTGTGTGGGACACGGACG GGCCTGGCAATGATGGGAAGCCGGAGCCCGTGTTGAGTTTTCTGCCCCAGAAGGACTGCACCAATGGCGT GAGCCTGCACCCTAGCCTGCCTCTCCTGGCCACTGCCTCCGGTCAGCGTGTGTTTCCTGAGCCCACAGAG AGTGGGGACGAAGGAGAGGAGCTGGGCCTTCCCTTGCTCTCCACGCGCCACGTCCACCTTGAATGTCGGC TTCAGCTCTGGTGGTGTGGGGGGGCGCCAGACTCCAGCATCCCTGATGATCACCAGGGCGAGAAAGGGCA GGGAGGAACGGAGGGAGGTGTGGGTGAGCTGATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227786 representing NM_001143992
Red=Cloning site Green=Tags(s) MKTLETQPLAPDCCPSDQDPAPAHPSPHASPMNKNADSELMPPPPERGDPPRLSPDPVAGSAVSQELREG DPVSLSTPLETEFGSPSELSPRIEEQELSENTSLPAEEANGSLSEEEANGPELGSGKAMEDTSGEPAAED EGDTAWNYSFSQLPRFLSGSWSEFSTQPENFLKGCKWAPDGSCILTNSADNILRIYNLPPELYHEGEQVE YAEMVPVLRMVEGDTIYDYCWYSLMSSAQPDTSYVASSSRENPIHIWDAFTGELRASFRAYNHLDELTAA HSLCFSPDGSQLFCGFNRTVRVFSTARPGRDCEVRATFAKKQGQSGIISCIAFSPAQPLYACGSYGRSLG LYAWDDGSPLALLGGHQGGITHLCFHPDGNRFFSGARKDAELLCWDLRQSGYPLWSLGREVTTNQRIYFD LDPTGQFLVSGSTSGAVSVWDTDGPGNDGKPEPVLSFLPQKDCTNGVSLHPSLPLLATASGQRVFPEPTE SGDEGEELGLPLLSTRHVHLECRLQLWWCGGAPDSSIPDDHQGEKGQGGTEGGVGELI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001143992 |
ORF Size | 1644 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001143992.2 |
RefSeq Size | 1896 bp |
RefSeq ORF | 1647 bp |
Locus ID | 55135 |
UniProt ID | Q9BUR4 |
Gene Summary | This gene encodes an essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex required for telomere synthesis. This protein is enriched in Cajal bodies, nuclear sites of RNP processing that are important for telomerase function. It interacts with dyskerin, TERT and TERC, other components of active telomerase, and with small Cajal body RNAs (scaRNAs), which are involved in modifying splicing RNAs. This mRNA also functions as a p53 antisense transcript, that regulates endogenous p53 mRNA levels and further induction of p53 protein by targeting the 5' untranslated region of p53 mRNA. Alternatively spliced transcript variants which differ only in the 5' UTR have been found for this gene. [provided by RefSeq, Mar 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227786 | WRAP53 (Myc-DDK-tagged)-Human WD repeat containing, antisense to TP53 (WRAP53), transcript variant 4 |
CNY 4,080.00 |
|
RC227786L3 | Lenti-ORF clone of WRAP53 (Myc-DDK-tagged)-Human WD repeat containing, antisense to TP53 (WRAP53), transcript variant 4 |
CNY 6,460.00 |
|
RC227786L4 | Lenti-ORF clone of WRAP53 (mGFP-tagged)-Human WD repeat containing, antisense to TP53 (WRAP53), transcript variant 4 |
CNY 6,460.00 |
|
SC325188 | WRAP53 (untagged)-Human WD repeat containing, antisense to TP53 (WRAP53), transcript variant 4 |
CNY 6,270.00 |