L1CAM (NM_001143963) Human Tagged ORF Clone
CAT#: RG227350
- TrueORF®
L1CAM (tGFP-tagged) - Human L1 cell adhesion molecule (L1CAM), transcript variant 3
ORF Plasmid: DDK
"NM_001143963" in other vectors (4)
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CNY 13,680.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227350 representing NM_001143963
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCGTGGCGCTGCGGTACGTGTGGCCTCTCCTCCTCTGCAGCCCCTGCCTGCTTATCCAGATCCCCG AGGAATTGATGGAGCCACCTGTCATCACGGAACAGTCTCCACGGCGCCTGGTTGTCTTCCCCACAGATGA CATCAGCCTCAAGTGTGAGGCCAGTGGCAAGCCCGAAGTGCAGTTCCGCTGGACGAGGGATGGTGTCCAC TTCAAACCCAAGGAAGAGCTGGGTGTGACCGTGTACCAGTCGCCCCACTCTGGCTCCTTCACCATCACGG GCAACAACAGCAACTTTGCTCAGAGGTTCCAGGGCATCTACCGCTGCTTTGCCAGCAATAAGCTGGGCAC CGCCATGTCCCATGAGATCCGGCTCATGGCCGAGGGTGCCCCCAAGTGGCCAAAGGAGACAGTGAAGCCC GTGGAGGTGGAGGAAGGGGAGTCAGTGGTTCTGCCTTGCAACCCTCCCCCAAGTGCAGAGCCTCTCCGGA TCTACTGGATGAACAGCAAGATCTTGCACATCAAGCAGGACGAGCGGGTGACGATGGGCCAGAACGGCAA CCTCTACTTTGCCAATGTGCTCACCTCCGACAACCACTCAGACTACATCTGCCACGCCCACTTCCCAGGC ACCAGGACCATCATTCAGAAGGAACCCATTGACCTCCGGGTCAAGGCCACCAACAGCATGATTGACAGGA AGCCGCGCCTGCTCTTCCCCACCAACTCCAGCAGCCACCTGGTGGCCTTGCAGGGGCAGCCATTGGTCCT GGAGTGCATCGCCGAGGGCTTTCCCACGCCCACCATCAAATGGCTGCGCCCCAGTGGCCCCATGCCAGCC GACCGTGTCACCTACCAGAACCACAACAAGACCCTGCAGCTGCTGAAAGTGGGCGAGGAGGATGATGGCG AGTACCGCTGCCTGGCCGAGAACTCACTGGGCAGTGCCCGGCATGCGTACTATGTCACCGTGGAGGCTGC CCCGTACTGGCTGCACAAGCCCCAGAGCCATCTATATGGGCCAGGAGAGACTGCCCGCCTGGACTGCCAA GTCCAGGGCAGGCCCCAACCAGAGGTCACCTGGAGAATCAACGGGATCCCTGTGGAGGAGCTGGCCAAAG ACCAGAAGTACCGGATTCAGCGTGGCGCCCTGATCCTGAGCAACGTGCAGCCCAGTGACACAATGGTGAC CCAATGTGAGGCCCGCAACCGGCACGGGCTCTTGCTGGCCAATGCCTACATCTACGTTGTCCAGCTGCCA GCCAAGATCCTGACTGCGGACAATCAGACGTACATGGCTGTCCAGGGCAGCACTGCCTACCTTCTGTGCA AGGCCTTCGGAGCGCCTGTGCCCAGTGTTCAGTGGCTGGACGAGGATGGGACAACAGTGCTTCAGGACGA ACGCTTCTTCCCCTATGCCAATGGGACCCTGGGCATTCGAGACCTCCAGGCCAATGACACCGGACGCTAC TTCTGCCTGGCTGCCAATGACCAAAACAATGTTACCATCATGGCTAACCTGAAGGTTAAAGATGCAACTC AGATCACTCAGGGGCCCCGCAGCACAATCGAGAAGAAAGGTTCCAGGGTGACCTTCACGTGCCAGGCCTC CTTTGACCCCTCCTTGCAGCCCAGCATCACCTGGCGTGGGGACGGTCGAGACCTCCAGGAGCTTGGGGAC AGTGACAAGTACTTCATAGAGGATGGGCGCCTGGTCATCCACAGCCTGGACTACAGCGACCAGGGCAACT ACAGCTGCGTGGCCAGTACCGAACTGGATGTGGTGGAGAGTAGGGCACAGCTCTTGGTGGTGGGGAGCCC TGGGCCGGTGCCACGGCTGGTGCTGTCCGACCTGCACCTGCTGACGCAGAGCCAGGTGCGCGTGTCCTGG AGTCCTGCAGAAGACCACAATGCCCCCATTGAGAAATATGACATTGAATTTGAGGACAAGGAAATGGCGC CTGAAAAATGGTACAGTCTGGGCAAGGTTCCAGGGAACCAGACCTCTACCACCCTCAAGCTGTCGCCCTA TGTCCACTACACCTTTAGGGTTACTGCCATAAACAAATATGGCCCCGGGGAGCCCAGCCCGGTCTCTGAG ACTGTGGTCACACCTGAGGCAGCCCCAGAGAAGAACCCTGTGGATGTGAAGGGGGAAGGAAATGAGACCA CCAATATGGTCATCACGTGGAAGCCGCTCCGGTGGATGGACTGGAACGCCCCCCAGGTTCAGTACCGCGT GCAGTGGCGCCCTCAGGGGACACGAGGGCCCTGGCAGGAGCAGATTGTCAGCGACCCCTTCCTGGTGGTG TCCAACACGTCCACCTTCGTGCCCTATGAGATCAAAGTCCAGGCCGTCAACAGCCAGGGCAAGGGACCAG AGCCCCAGGTCACTATCGGCTACTCTGGAGAGGACTACCCCCAGGCAATCCCTGAGCTGGAAGGCATTGA AATCCTCAACTCAAGTGCCGTGCTGGTCAAGTGGCGGCCGGTGGACCTGGCCCAGGTCAAGGGCCACCTC CGCGGATACAATGTGACGTACTGGAGGGAGGGCAGTCAGAGGAAGCACAGCAAGAGACATATCCACAAAG ACCATGTGGTGGTGCCCGCCAACACCACCAGTGTCATCCTCAGTGGCTTGCGGCCCTATAGCTCCTACCA CCTGGAGGTGCAGGCCTTTAACGGGCGAGGATCGGGGCCCGCCAGCGAGTTCACCTTCAGCACCCCAGAG GGAGTGCCTGGCCACCCCGAGGCGTTGCACCTGGAGTGCCAGTCGAACACCAGCCTGCTGCTGCGCTGGC AGCCCCCACTCAGCCACAACGGCGTGCTCACCGGCTACGTGCTCTCCTACCACCCCCTGGATGAGGGGGG CAAGGGGCAACTGTCCTTCAACCTTCGGGACCCCGAACTTCGGACACACAACCTGACCGATCTCAGCCCC CACCTGCGGTACCGCTTCCAGCTTCAGGCCACCACCAAAGAGGGCCCTGGTGAAGCCATCGTACGGGAAG GAGGCACTATGGCCTTGTCTGGGATCTCAGATTTTGGCAACATCTCAGCCACAGCGGGTGAAAACTACAG TGTCGTCTCCTGGGTCCCCAAGGAGGGCCAGTGCAACTTCAGGTTCCATATCTTGTTCAAAGCCTTGGGA GAAGAGAAGGGTGGGGCTTCCCTTTCGCCACAGTATGTCAGCTACAACCAGAGCTCCTACACGCAGTGGG ACCTGCAGCCTGACACTGACTACGAGATCCACTTGTTTAAGGAGAGGATGTTCCGGCACCAAATGGCTGT GAAGACCAATGGCACAGGCCGCGTGAGGCTCCCTCCTGCTGGCTTCGCCACTGAGGGCTGGTTCATCGGC TTTGTGAGTGCCATCATCCTCCTGCTCCTCGTCCTGCTCATCCTCTGCTTCATCAAGCGCAGCAAGGGCG GCAAATACTCAGTGAAGGATAAGGAGGACACCCAGGTGGACTCTGAGGCCCGACCGATGAAAGATGAGAC CTTCGGCGAGTACAGTGACAACGAGGAGAAGGCCTTTGGCAGCAGCCAGCCATCGCTCAACGGGGACATC AAGCCCCTGGGCAGTGACGACAGCCTGGCCGATTATGGGGGCAGCGTGGATGTTCAGTTCAACGAGGATG GTTCGTTCATTGGCCAGTACAGTGGCAAGAAGGAGAAGGAGGCGGCAGGGGGCAATGACAGCTCAGGGGC CACTTCCCCCATCAACCCTGCCGTGGCCCTAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227350 representing NM_001143963
Red=Cloning site Green=Tags(s) MVVALRYVWPLLLCSPCLLIQIPEELMEPPVITEQSPRRLVVFPTDDISLKCEASGKPEVQFRWTRDGVH FKPKEELGVTVYQSPHSGSFTITGNNSNFAQRFQGIYRCFASNKLGTAMSHEIRLMAEGAPKWPKETVKP VEVEEGESVVLPCNPPPSAEPLRIYWMNSKILHIKQDERVTMGQNGNLYFANVLTSDNHSDYICHAHFPG TRTIIQKEPIDLRVKATNSMIDRKPRLLFPTNSSSHLVALQGQPLVLECIAEGFPTPTIKWLRPSGPMPA DRVTYQNHNKTLQLLKVGEEDDGEYRCLAENSLGSARHAYYVTVEAAPYWLHKPQSHLYGPGETARLDCQ VQGRPQPEVTWRINGIPVEELAKDQKYRIQRGALILSNVQPSDTMVTQCEARNRHGLLLANAYIYVVQLP AKILTADNQTYMAVQGSTAYLLCKAFGAPVPSVQWLDEDGTTVLQDERFFPYANGTLGIRDLQANDTGRY FCLAANDQNNVTIMANLKVKDATQITQGPRSTIEKKGSRVTFTCQASFDPSLQPSITWRGDGRDLQELGD SDKYFIEDGRLVIHSLDYSDQGNYSCVASTELDVVESRAQLLVVGSPGPVPRLVLSDLHLLTQSQVRVSW SPAEDHNAPIEKYDIEFEDKEMAPEKWYSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPSPVSE TVVTPEAAPEKNPVDVKGEGNETTNMVITWKPLRWMDWNAPQVQYRVQWRPQGTRGPWQEQIVSDPFLVV SNTSTFVPYEIKVQAVNSQGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHL RGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPE GVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSP HLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALG EEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATEGWFIG FVSAIILLLLVLLILCFIKRSKGGKYSVKDKEDTQVDSEARPMKDETFGEYSDNEEKAFGSSQPSLNGDI KPLGSDDSLADYGGSVDVQFNEDGSFIGQYSGKKEKEAAGGNDSSGATSPINPAVALE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001143963 |
ORF Size | 3744 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001143963.2 |
RefSeq Size | 5019 bp |
RefSeq ORF | 3747 bp |
Locus ID | 3897 |
UniProt ID | P32004 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
Protein Pathways | Axon guidance, Cell adhesion molecules (CAMs) |
Gene Summary | The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227350 | L1CAM (Myc-DDK-tagged)-Human L1 cell adhesion molecule (L1CAM), transcript variant 3 |
CNY 12,450.00 |
|
RC227350L3 | Lenti-ORF clone of L1CAM (Myc-DDK-tagged)-Human L1 cell adhesion molecule (L1CAM), transcript variant 3 |
CNY 14,350.00 |
|
RC227350L4 | Lenti-ORF clone of L1CAM (mGFP-tagged)-Human L1 cell adhesion molecule (L1CAM), transcript variant 3 |
CNY 14,350.00 |
|
SC326363 | L1CAM (untagged)-Human L1 cell adhesion molecule (L1CAM), transcript variant 3 |
CNY 24,040.00 |