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C20orf31 (EDEM2) (NM_001145025) Human Tagged ORF Clone
CAT＃: RG227309
- TrueORF®
EDEM2 (tGFP-tagged) - Human ER degradation enhancer, mannosidase alpha-like 2 (EDEM2), transcript variant 2
ORF Plasmid: DDK
"NM_001145025" in other vectors (4)
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CNY 5,230.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | bA4204.1; C20orf31; C20orf49 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG227309 representing NM_001145025
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTTCCGGCTGCTCATCCCGCTCGGCCTCCTGTGCGCGCTGCTGCCTCAGCACCATGGTGCGCCAG GTCCCGACGGCTCCGCGCCAGATCCCGCCCACTACAGTTTTTCTCTGACTCTAATTGATGCACTGGACAC CTTGCTGATTTTGGGGAATGTCTCAGAATTCCAAAGAGTGGTTGAAGTGCTCCAGGACAGCGTGGACTTT GATATTGATGTGAACGCCTCTGTGTTTGAAACAAACATTCGAGTGGTAGGAGGACTCCTGTCTGCTCATC TGCTCTCCAAGAAGGCTGGGGTGGAAGTAGAGGCTGGATGGCCCTGTTCCGGGCCTCTCCTGAGAATGGC TGAGGAGGCGGCCCGAAAACTCCTCCCAGCCTTTCAGACCCCCACTGGCATGCCATATGGAACAGTGAAC TTACTTCATGGCGTGAACCCAGGAGAGACCCCTGTCACCTGTACGGCAGGGATTGGGACCTTCATTGTTG AATTTGCCACCCTGAGCAGCCTCACTGGTGACCCGGTGTTCGAAGATGTGGCCAGAGTGGCTTTGATGCG CCTCTGGGAGAGCCGGTCAGATATCGGGCTGGTCGGCAACCACATTGATGTGCTCACTGGCAAGTGGGTG GCCCAGGACGCAGGCATCGGGGCTGGCGTGGACTCCTACTTTGAGTACTTGGTGAAAGGAGCCATCCTGC TTCAGGATAAGAAGCTCATGGCCATGTTCCTAGAGTATAACAAAGCCATCCGGAACTACACCCGCTTCGA TGACTGGTACCTGTGGGTTCAGATGTACAAGGGGACTGTGTCCATGCCAGTCTTCCAGTCCTTGGAGGCC TACTGGCCTGGTCTTCAGAGCCTCATTGGAGACATTGACAATGCCATGAGGACCTTCCTCAACTACTACA CTGTATGGAAGCAGTTTGGGGGGCTCCCGGAATTCTACAACATTCCTCAGGGATACACAGTGGAGAAGCG AGAGGGCTACCCACTTCGGCCAGAACTTATTGAAAGCGCAATGTACCTCTACCGTGCCACGGGGGATCCC ACCCTCCTAGAACTCGGAAGAGATGCTGTGGAATCCATTGAAAAAATCAGCAAGGTGGAGTGCGGATTTG CAACAATCAAAGATCTGCGAGACCACAAGCTGGACAACCGCATGGAGTCGTTCTTCCTGGCCGAGACTGT GAAATACCTCTACCTCCTGTTTGACCCAACCAACTTCATCCACAACAATGGGTCCACCTTCGACGCGGTG ATCACCCCCTATGGGGAGTGCATCCTGGGGGCTGGGGGGTACATCTTCAACACAGAAGCTCACCCCATCG ACCCTGCCGCCCTGCACTGCTGCCAGAGGCTGAAGGAAGAGCAGTGGGAGGTGGAGGACTTGATGAGGGA ATTCTACTCTCTCAAACGGAGCAGGTCGAAATTTCAGAAAAACACTGTTAGTTCGGGGCCATGGGAACCT CCAGCAAGGCCAGGAACACTCTTCTCACCAGAAAACCATGACCAGGCAAGGGAGAGGAAGCCTGCCAAAC AGAAGGTCCCACTTCTCAGCTGCCCCAGTCAGCCCTTCACCTCCAAGTTGGCATTACTGGGACAGGTTTT CCTAGACTCCTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227309 representing NM_001145025
Red=Cloning site Green=Tags(s) MPFRLLIPLGLLCALLPQHHGAPGPDGSAPDPAHYSFSLTLIDALDTLLILGNVSEFQRVVEVLQDSVDF DIDVNASVFETNIRVVGGLLSAHLLSKKAGVEVEAGWPCSGPLLRMAEEAARKLLPAFQTPTGMPYGTVN LLHGVNPGETPVTCTAGIGTFIVEFATLSSLTGDPVFEDVARVALMRLWESRSDIGLVGNHIDVLTGKWV AQDAGIGAGVDSYFEYLVKGAILLQDKKLMAMFLEYNKAIRNYTRFDDWYLWVQMYKGTVSMPVFQSLEA YWPGLQSLIGDIDNAMRTFLNYYTVWKQFGGLPEFYNIPQGYTVEKREGYPLRPELIESAMYLYRATGDP TLLELGRDAVESIEKISKVECGFATIKDLRDHKLDNRMESFFLAETVKYLYLLFDPTNFIHNNGSTFDAV ITPYGECILGAGGYIFNTEAHPIDPAALHCCQRLKEEQWEVEDLMREFYSLKRSRSKFQKNTVSSGPWEP PARPGTLFSPENHDQARERKPAKQKVPLLSCPSQPFTSKLALLGQVFLDSS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145025 |
| ORF Size | 1623 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145025.2 |
| RefSeq Size | 1807 bp |
| RefSeq ORF | 1626 bp |
| Locus ID | 55741 |
| UniProt ID | Q9BV94 |
| Protein Families | Secreted Protein |
| Gene Summary | In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed 15537790]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227309 | EDEM2 (Myc-DDK-tagged)-Human ER degradation enhancer, mannosidase alpha-like 2 (EDEM2), transcript variant 2 |
CNY 4,032.00 |
|
| RC227309L3 | Lenti-ORF clone of EDEM2 (Myc-DDK-tagged)-Human ER degradation enhancer, mannosidase alpha-like 2 (EDEM2), transcript variant 2 |
CNY 6,650.00 |
|
| RC227309L4 | Lenti-ORF clone of EDEM2 (mGFP-tagged)-Human ER degradation enhancer, mannosidase alpha-like 2 (EDEM2), transcript variant 2 |
CNY 6,650.00 |
|
| SC326050 | EDEM2 (untagged)-Human ER degradation enhancer, mannosidase alpha-like 2 (EDEM2), transcript variant 2 |
CNY 8,650.00 |
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