ACAD10 (NM_001136538) Human Tagged ORF Clone
CAT#: RG227025
- TrueORF®
ACAD10 (tGFP-tagged) - Human acyl-CoA dehydrogenase family, member 10 (ACAD10), transcript variant 1
ORF Plasmid: DDK
"NM_001136538" in other vectors (4)
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CNY 11,880.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227025 representing NM_001136538
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGTCAGGAGCTGTTTCCAGTCCCCCCGTCTCCAGTGGGTGTGGAGAACAGCCTTCCTGAAACACA CCCAGCGCAGGCACCAGGGGTCCCACCGATGGACACACCTTGGAGGCAGCACCTACAGAGCGGTGATTTT CGACATGGGCGGAGTTCTCATTCCTTCTCCAGGGAGAGTCGCTGCAGAATGGGAGGTACAGAATCGTATC CCTTCTGGAACTATATTAAAGGCCTTGATGGAAGGTGGTGAAAATGGGCCCTGGATGAGATTTATGAGAG CAGAAATAACAGCAGAGGGTTTTTTACGAGAATTTGGGAGACTTTGCTCTGAAATGTTAAAGACCTCCGT GCCTGTGGACTCATTTTTCTCTCTGTTGACCAGTGAGCGAGTGGCAAAGCAGTTCCCAGTGATGACTGAG GCCATAACTCAAATTCGGGCAAAAGGTCTTCAGACTGCAGTCTTGAGCAATAATTTTTATCTTCCCAACC AGAAAAGCTTTTTGCCCCTGGACCGGAAACAGTTTGATGTGATTGTGGAGTCCTGCATGGAAGGGATCTG TAAGCCAGACCCTAGGATCTACAAGCTGTGCTTGGAGCAGCTCGGCCTGCAGCCCTCTGAGTCCATCTTT CTTGATGACCTTGGAACAAATCTAAAAGAAGCTGCCAGACTTGGTATTCACACCATTAAGAGACAGGGTT TTGCCGTGTTGCCCAAGCTGGTGTCGAACTCCTGGGCTCAAGCGATCTACCCACCTTACCCTCCCAAAGT GGTGAGATTACAGGTTAATGACCCAGAGACTGCAGTAAAGGAATTAGAAGCTCTCTTGGGTTTTACATTG AGAGTAGGTGTTCCAAACACTCGGCCTGTGAAAAAGACGATGGAAATTCCGAAAGATTCCTTGCAGAAGT ACCTCAAAGACTTACTGGGTATCCAGACCACAGGCCCATTGGAACTACTTCAGTTTGATCACGGGCAGTC AAATCCAACTTACTACATCAGGCTGGCTAATCGTGATCTAGTTCTGAGGAAGAAGCCCCCAGGGACACTC CTTCCATCTGCCCATGCCATAGAGAGGGAGTTCAGGATTATGAAAGCCCTTGCAAATGCTGGAGTACCTG TCCCTAACGTTCTTGATCTCTGTGAAGATTCAAGTGTCATTGGCACCCCCTTCTATGTGATGGAGTACTG CCCAGGTCTCATCTACAAAGACCCTTCCCTGCCAGGCTTGGAGCCCAGCCACAGACGAGCCATATACACT GCCATGAACACAGTCCTGTGCAAAATTCACAGTGTGGATCTGCAGGCTGTGGGACTTGAAGACTATGGGA AGCAAGGGGACTATATTCCACGCCAGGTACGAACCTGGGTTAAGCAGTATCGAGCTTCCGAAACTAGCAC CATCCCAGCCATGGAGAGGCTGATCGAATGGCTGCCCCTCCATCTTCCCCGTCAGCAGAGGACCACAGTG GTGCACGGGGACTTCAGGCTCGACAACCTGGTGTTTCATCCAGAAGAGCCAGAGGTGCTTGCTGTCCTTG ACTGGGAACTTTCTACCTTGGGCGACCCCCTTGCTGATGTGGCCTACAGCTGCCTGGCTCATTACCTGCC ATCCAGTTTTCCCGTGCTGAGAGGTATTAATGACTGTGACTTGACACAGCTGGGAATCCCTGCTGCAGAG GAGTATTTCAGGATGTACTGTCTCCAAATGGGGCTCCCTCCCACTGAGAACTGGAACTTCTATATGGCTT TTTCCTTTTTCCGTGTGGCTGCAATCCTACAGGGAGTCTACAAGCGATCACTCACAGGGCAAGCAAGCTC CACATATGCGGAACAAACTGGAAAGCTGACCGAATTTGTGTCTAACCTGGCGTGGGATTTCGCAGTCAAA GAAGGGTTCCGGGTTTTCAAAGAGATGCCCTTCACAAATCCGTTAACAAGGTCCTACCACACGTGGGCCA GGCCCCAGTCCCAGTGGTGCCCCACAGGCAGCAGGAGTTATAGCTCCGTTCCAGAAGCTTCCCCAGCTCA TACCTCAAGGGGAGGTCTGGTTATCTCTCCAGAGAGCCTCTCTCCACCTGTCAGAGAGCTGTATCACCGG CTGAAGCACTTCATGGAGCAACGTGTGTACCCTGCAGAGCCAGAGCTGCAGAGTCACCAGGCCTCAGCAG CCAGGTGGAGCCCCTCCCCACTGATCGAAGACCTCAAGGAGAAAGCCAAAGCTGAAGGACTTTGGAACCT TTTCCTACCCTTAGAGGCTGATCCCGAGAAAAAATACGGAGCAGGACTGACCAATGTGGAATATGCACAT CTGTGTGAGCTCATGGGCACGTCCCTGTATGCCCCCGAGGTATGTAACTGCTCTGCGCCTGACACGGGCA ACATGGAGCTGCTGGTGAGGTATGGCACCGAAGCGCAGAAGGCTCGCTGGCTGATTCCTCTGCTGGAGGG GAAAGCCCGCTCCTGTTTTGCTATGACCGAGCCCCAGGTTGCCTCTTCAGATGCCACCAACATTGAGGCT TCCATCAGAGAGGAGGACAGCTTCTATGTCATAAACGGTCACAAATGGTGGATCACAGGCATCCTGGATC CTCGTTGCCAACTCTGTGTGTTTATGGGAAAAACAGACCCACATGCACCAAGACACCGGCAGCAGTCTGT GCTCTTGGTTCCCATGGATACCCCAGGGATAAAAATCATCCGGCCTCTGACGGTGTATGGACTGGAAGAT GCACCAGGTGGCCATGGTGAAGTCCGATTTGAGCACGTGCGTGTGCCCAAAGAGAACATGGTCCTGGGCC CTGGCCGAGGCTTTGAGATCGCCCAGGGCAGACTGGGCCCCGGCAGGATCCATCACTGCATGAGGCTGAT CGGGTTCTCAGAGAGGGCCCTGGCACTCATGAAGGCCCGCGTGAAGTCCCGCTTGGCTTTTGGGAAGCCC CTGGTGGAGCAGGGCACAGTGCTGGCGGACATCGCGCAGTCGCGCGTGGAGATTGAGCAGGCACGGCTGC TGGTGCTGAGAGCTGCCCACCTCATGGACCTGGCAGGAAACAAGGCTGCAGCCTTGGATATAGCCATGAT TAAAATGGTCGCCCCGTCCATGGCCTCCCGAGTGATTGATCGTGCGATTCAGGCCTTTGGAGCAGCAGGC CTGAGCAGCGACTACCCACTGGCTCAGTTCTTCACCTGGGCCCGAGCCCTGCGCTTTGCCGACGGCCCTG ACGAGGTGCACCGGGCCACGGTGGCCAAGCTAGAGCTGAAGCACCGCATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227025 representing NM_001136538
Red=Cloning site Green=Tags(s) MCVRSCFQSPRLQWVWRTAFLKHTQRRHQGSHRWTHLGGSTYRAVIFDMGGVLIPSPGRVAAEWEVQNRI PSGTILKALMEGGENGPWMRFMRAEITAEGFLREFGRLCSEMLKTSVPVDSFFSLLTSERVAKQFPVMTE AITQIRAKGLQTAVLSNNFYLPNQKSFLPLDRKQFDVIVESCMEGICKPDPRIYKLCLEQLGLQPSESIF LDDLGTNLKEAARLGIHTIKRQGFAVLPKLVSNSWAQAIYPPYPPKVVRLQVNDPETAVKELEALLGFTL RVGVPNTRPVKKTMEIPKDSLQKYLKDLLGIQTTGPLELLQFDHGQSNPTYYIRLANRDLVLRKKPPGTL LPSAHAIEREFRIMKALANAGVPVPNVLDLCEDSSVIGTPFYVMEYCPGLIYKDPSLPGLEPSHRRAIYT AMNTVLCKIHSVDLQAVGLEDYGKQGDYIPRQVRTWVKQYRASETSTIPAMERLIEWLPLHLPRQQRTTV VHGDFRLDNLVFHPEEPEVLAVLDWELSTLGDPLADVAYSCLAHYLPSSFPVLRGINDCDLTQLGIPAAE EYFRMYCLQMGLPPTENWNFYMAFSFFRVAAILQGVYKRSLTGQASSTYAEQTGKLTEFVSNLAWDFAVK EGFRVFKEMPFTNPLTRSYHTWARPQSQWCPTGSRSYSSVPEASPAHTSRGGLVISPESLSPPVRELYHR LKHFMEQRVYPAEPELQSHQASAARWSPSPLIEDLKEKAKAEGLWNLFLPLEADPEKKYGAGLTNVEYAH LCELMGTSLYAPEVCNCSAPDTGNMELLVRYGTEAQKARWLIPLLEGKARSCFAMTEPQVASSDATNIEA SIREEDSFYVINGHKWWITGILDPRCQLCVFMGKTDPHAPRHRQQSVLLVPMDTPGIKIIRPLTVYGLED APGGHGEVRFEHVRVPKENMVLGPGRGFEIAQGRLGPGRIHHCMRLIGFSERALALMKARVKSRLAFGKP LVEQGTVLADIAQSRVEIEQARLLVLRAAHLMDLAGNKAAALDIAMIKMVAPSMASRVIDRAIQAFGAAG LSSDYPLAQFFTWARALRFADGPDEVHRATVAKLELKHRI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001136538 |
ORF Size | 3270 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001136538.2 |
RefSeq Size | 4124 bp |
RefSeq ORF | 3273 bp |
Locus ID | 80724 |
UniProt ID | Q6JQN1 |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes a member of the acyl-CoA dehydrogenase family of enzymes (ACADs), which participate in the beta-oxidation of fatty acids in mitochondria. The encoded enzyme contains a hydrolase domain at the N-terminal portion, a serine/threonine protein kinase catlytic domain in the central region, and a conserved ACAD domain at the C-terminus. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Nov 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227025 | ACAD10 (Myc-DDK-tagged)-Human acyl-CoA dehydrogenase family, member 10 (ACAD10), transcript variant 1 |
CNY 10,830.00 |
|
RC227025L3 | Lenti-ORF clone of ACAD10 (Myc-DDK-tagged)-Human acyl-CoA dehydrogenase family, member 10 (ACAD10), transcript variant 1 |
CNY 12,730.00 |
|
RC227025L4 | Lenti-ORF clone of ACAD10 (mGFP-tagged)-Human acyl-CoA dehydrogenase family, member 10 (ACAD10), transcript variant 1 |
CNY 12,730.00 |
|
SC326336 | ACAD10 (untagged)-Human acyl-CoA dehydrogenase family, member 10 (ACAD10), transcript variant 1 |
CNY 20,900.00 |