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ANO6 (NM_001142678) Human Tagged ORF Clone
CAT＃: RG226624
- TrueORF®
ANO6 (tGFP-tagged) - Human anoctamin 6 (ANO6), transcript variant 2
ORF Plasmid: DDK
"NM_001142678" in other vectors (4)
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CNY 10,360.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BDPLT7; SCTS; TMEM16F |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226624 representing NM_001142678
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTTGTGCTGCTGTGTTGGAAAACCTTGGACAGACAATTGTCCCCGATTTGGGATCACTGGAAAGTC AGCATGATTTTCGAACCCCGGAGTTTGAAGAATTTAATGGAAAACCTGACTCCCTCTTTTTTAATGATGG CCAGCGAAGAATTGACTTTGTTCTAGTATATGAGGATGAAAGCAGAAAAGAGACCAATAAAAAGGGTACA AATGAAAAACAAAGGAGGAAAAGACAAGCATACGAATCTAACCTTATCTGTCATGGCCTGCAGTTAGAAG CAACAAGATCAGTATTGGATGACAAGCTTGTATTTGTAAAAGTACACGCACCATGGGAGGTGTTATGTAC GTATGCTGAGATAATGCACATCAAATTGCCTCTGAAACCCAATGATCTGAAAAACCGGTCCTCAGCCTTT GGTACACTCAACTGGTTTACCAAAGTCCTCAGTGTAGACGAAAGCATCATCAAGCCAGAGCAAGAGTTTT TCACTGCCCCATTTGAGAAGAACCGGATGAATGATTTTTACATAGTTGATAGAGATGCTTTCTTCAATCC AGCCACCAGAAGCCGCATTGTTTACTTCATCCTCTCTCGGGTCAAGTATCAAGTGATAAACAATGTTAGC AAGTTTGGGATCAACAGACTTGTAAACTCTGGGATCTACAAGGCAGCTTTCCCACTCCATGATTGCAAAT TCCGCCGTCAGTCAGAGGATCCCAGCTGCCCTAATGAACGGTACCTTCTGTACAGAGAATGGGCTCATCC TCGAAGCATATACAAAAAGCAGCCCTTGGATCTTATCAGGAAATACTATGGAGAGAAGATTGGAATCTAC TTTGCTTGGCTGGGCTATTACACTCAGATGCTTCTCCTGGCCGCAGTTGTAGGAGTGGCTTGCTTTCTCT ATGGATATCTTAATCAAGATAACTGTACATGGAGCAAAGAAGTTTGTCATCCTGATATTGGTGGCAAGAT CATAATGTGTCCTCAGTGTGATAGGCTTTGTCCATTCTGGAAACTCAATATTACTTGCGAGTCCTCAAAG AAATTGTGCATCTTCGACAGTTTTGGAACCCTGGTCTTTGCAGTATTTATGGGAGTATGGGTTACCTTGT TTTTGGAGTTTTGGAAGCGACGCCAGGCAGAACTTGAGTATGAATGGGATACTGTTGAGTTACAGCAGGA AGAACAAGCCCGACCAGAATACGAAGCACGATGTACTCACGTAGTGATAAATGAGATTACTCAGGAAGAA GAACGCATTCCCTTTACTGCCTGGGGAAAATGTATACGGATAACCCTCTGTGCCAGTGCTGTCTTTTTCT GGATCCTATTGATCATCGCTTCAGTTATTGGGATCATTGTCTATAGGCTCTCGGTGTTCATTGTATTTTC TGCAAAACTTCCCAAGAACATTAATGGAACAGACCCAATCCAGAAATACCTGACTCCACAGACAGCCACG TCCATCACGGCCTCCATCATCAGCTTTATAATTATCATGATTCTGAACACCATATATGAAAAAGTGGCAA TTATGATTACTAACTTCGAACTCCCAAGGACCCAGACTGATTATGAGAACAGCCTCACCATGAAGATGTT CTTATTCCAGTTTGTCAACTACTACTCTTCATGCTTCTACATAGCATTCTTTAAGGGCAAATTTGTAGGC TATCCAGGAGACCCAGTTTATTGGTTGGGAAAATACAGAAATGAAGAGTGTGACCCAGGTGGCTGTCTTC TTGAACTGACAACTCAGCTGACAATAATCATGGGAGGAAAAGCAATCTGGAATAACATACAAGAAGTATT ATTGCCCTGGATCATGAATCTAATTGGGCGATTTCACAGAGTTTCTGGATCAGAAAAGATAACCCCACGA TGGGAACAGGACTACCATCTGCAGCCTATGGGCAAACTGGGATTATTTTATGAATATCTTGAAATGATTA TTCAGTTTGGGTTCGTCACCTTATTTGTGGCCTCTTTTCCACTGGCCCCTCTGTTGGCTCTCGTGAACAA TATATTGGAAATAAGAGTGGACGCATGGAAACTGACCACCCAGTTTAGACGCCTGGTACCAGAGAAAGCC CAAGACATTGGAGCATGGCAGCCCATCATGCAAGGAATAGCAATTCTGGCTGTGGTGACCAATGCCATGA TCATAGCTTTCACGTCGGACATGATCCCCCGCCTAGTGTACTACTGGTCCTTCTCCGTCCCTCCCTACGG GGACCACACTTCCTACACCATGGAAGGGTACATCAACAACACTCTCTCCATCTTCAAAGTCGCAGACTTC AAAAACAAAAGCAAGGGAAACCCGTACTCTGACCTGGGTAACCATACCACATGCAGGTATCGTGATTTCC GATACCCACCTGGACACCCCCAGGAGTATAAACACAACATCTACTATTGGCATGTGATTGCAGCCAAGCT GGCTTTTATCATTGTCATGGAGCACGTCATCTACTCTGTGAAATTTTTCATTTCATATGCAATTCCCGAT GTATCAAAACGCACAAAGAGCAAGATCCAGAGAGAAAAATACCTAACCCAAAAGCTTCTTCATGAGAATC ACCTCAAAGATATGACGAAAAATATGGGGGTGATAGCTGAGCGGATGATAGAAGCAGTAGATAACAATTT ACGGCCAAAATCAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226624 representing NM_001142678
Red=Cloning site Green=Tags(s) MFCAAVLENLGQTIVPDLGSLESQHDFRTPEFEEFNGKPDSLFFNDGQRRIDFVLVYEDESRKETNKKGT NEKQRRKRQAYESNLICHGLQLEATRSVLDDKLVFVKVHAPWEVLCTYAEIMHIKLPLKPNDLKNRSSAF GTLNWFTKVLSVDESIIKPEQEFFTAPFEKNRMNDFYIVDRDAFFNPATRSRIVYFILSRVKYQVINNVS KFGINRLVNSGIYKAAFPLHDCKFRRQSEDPSCPNERYLLYREWAHPRSIYKKQPLDLIRKYYGEKIGIY FAWLGYYTQMLLLAAVVGVACFLYGYLNQDNCTWSKEVCHPDIGGKIIMCPQCDRLCPFWKLNITCESSK KLCIFDSFGTLVFAVFMGVWVTLFLEFWKRRQAELEYEWDTVELQQEEQARPEYEARCTHVVINEITQEE ERIPFTAWGKCIRITLCASAVFFWILLIIASVIGIIVYRLSVFIVFSAKLPKNINGTDPIQKYLTPQTAT SITASIISFIIIMILNTIYEKVAIMITNFELPRTQTDYENSLTMKMFLFQFVNYYSSCFYIAFFKGKFVG YPGDPVYWLGKYRNEECDPGGCLLELTTQLTIIMGGKAIWNNIQEVLLPWIMNLIGRFHRVSGSEKITPR WEQDYHLQPMGKLGLFYEYLEMIIQFGFVTLFVASFPLAPLLALVNNILEIRVDAWKLTTQFRRLVPEKA QDIGAWQPIMQGIAILAVVTNAMIIAFTSDMIPRLVYYWSFSVPPYGDHTSYTMEGYINNTLSIFKVADF KNKSKGNPYSDLGNHTTCRYRDFRYPPGHPQEYKHNIYYWHVIAAKLAFIIVMEHVIYSVKFFISYAIPD VSKRTKSKIQREKYLTQKLLHENHLKDMTKNMGVIAERMIEAVDNNLRPKSE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001142678 |
| ORF Size | 2676 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001142678.2 |
| RefSeq Size | 5980 bp |
| RefSeq ORF | 2679 bp |
| Locus ID | 196527 |
| UniProt ID | Q4KMQ2 |
| Protein Families | Transmembrane |
| Gene Summary | This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226624 | ANO6 (Myc-DDK-tagged)-Human anoctamin 6 (ANO6), transcript variant 2 |
CNY 9,410.00 |
|
| RC226624L3 | Lenti-ORF clone of ANO6 (Myc-DDK-tagged)-Human anoctamin 6 (ANO6), transcript variant 2 |
CNY 11,310.00 |
|
| RC226624L4 | Lenti-ORF clone of ANO6 (mGFP-tagged)-Human anoctamin 6 (ANO6), transcript variant 2 |
CNY 11,310.00 |
|
| SC326274 | ANO6 (untagged)-Human anoctamin 6 (ANO6), transcript variant 2 |
CNY 9,808.00 |
