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Reticulon 2 (RTN2) (NM_206900) Human Tagged ORF Clone
CAT＃: RG224942
- TrueORF®
RTN2 (tGFP-tagged) - Human reticulon 2 (RTN2), transcript variant 2
ORF Plasmid: DDK
"NM_206900" in other vectors (4)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | NSP2; NSPL1; NSPLI; SPG12 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224942 representing NM_206900
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAGGTCCTGCCGGTCTTCGCCCACTGCAAAGAAGCTCCGTCTACAGCCTCCTCAACTCCTGATT CCACAGAAGGAGGGAACGACGACTCTGATTTTCGAGAGCTGCACACAGCCCGGGAATTCTCAGAGGAGGA CGAGGAGGAGACCACGTCGCAGGACTGGGGCACCCCCCGGGAGCTGACCTTCTCCTACATCGCCTTTGAT GGTGTAGTGGGCTCCGGGGGCCGCAGGGATTCAACTGCCCGCCGCCCCCGCCCCCAGGGCCGCTCAGTCT CGGAACCACGAGACCAGCACCCTCAGCCCAGCCTGGGCGACAGCTTGGAGAGCATCCCCAGCCTGAGCCA ATCCCCGGAGCCTGGACGACGGGGTGATCCTGACACCGCGCCTCCATCCGAGCGCCCTCTGGAAGACCTG AGGCTTCGGTTGGACCATCTGGGCTGGGTGGCCCGGGGAACGGGATCCGGGGAGGACTCTTCCACCAGCA GCTCCACCCCGCTGGAAGACGAAGAACCCCAAGAACCCAACAGATTGGAGACAGGAGAAGCTGGGGAAGA ACTGGACCTACGACTCCGACTTGCTCAGCCCTCATCGCCCGAGGTCTTGACTCCCCAGCTCAGTCCGGGC TCTGGGACACCCCAGGCCGGTACTCCGTCCCCATCCCGATCGCGAGATTCGAACTCTGGGCCCGAAGAGC CATTGCTGGAAGAGGAAGAAAAGCAGTGGGGGCCACTGGAGCGAGAGCCAGTAAGGGGACAGTGCCTCGA TAGCACGGACCAATTAGAATTCACGGTGGAGCCACGCCTTCTAGTGGCGGACCTGCTGTACTGGAAGGAC ACGAGGACGTCAGGAGTGGTCTTCACAGGCCTGATGGTCTCCCTCCTCTGCCTCCTGCACTTTAGCATCG TGTCCGTGGCCGCGCACTTGGCTCTGTTGCTGCTCTGCGGCACCATCTCTCTCAGGGTTTACCGCAAAGT GCTGCAGGCCGTGCACCGGGGGGATGGAGCCAACCCTTTCCAGGCCTACCTGGATGTGGACCTCACCCTG ACTCGGGAGCAGACGGAACGTTTGTCCCACCAGATCACCTCCCGCGTGGTCTCGGCGGCCACGCAGCTGC GGCACTTCTTCCTGGTAGAAGACCTCGTGGATTCCCTCAAGCTGGCCCTCCTCTTCTACATCTTGACCTT CGTGGGTGCCATCTTCAATGGTTTGACTCTTCTCATTCTGGGAGTGATTGGTCTATTCACCATCCCCCTG CTGTACCGGCAGCACCAGGCTCAGATCGACCAATATGTGGGGTTGGTGACCAATCAGTTGAGCCACATCA AAGCTAAGATCCGAGCTAAAATCCCAGGGACCGGAGCCCTGGCCTCTGCAGCAGCCGCAGTCTCCGGATC CAAAGCCAAAGCCGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224942 representing NM_206900
Red=Cloning site Green=Tags(s) MGQVLPVFAHCKEAPSTASSTPDSTEGGNDDSDFRELHTAREFSEEDEEETTSQDWGTPRELTFSYIAFD GVVGSGGRRDSTARRPRPQGRSVSEPRDQHPQPSLGDSLESIPSLSQSPEPGRRGDPDTAPPSERPLEDL RLRLDHLGWVARGTGSGEDSSTSSSTPLEDEEPQEPNRLETGEAGEELDLRLRLAQPSSPEVLTPQLSPG SGTPQAGTPSPSRSRDSNSGPEEPLLEEEEKQWGPLEREPVRGQCLDSTDQLEFTVEPRLLVADLLYWKD TRTSGVVFTGLMVSLLCLLHFSIVSVAAHLALLLLCGTISLRVYRKVLQAVHRGDGANPFQAYLDVDLTL TREQTERLSHQITSRVVSAATQLRHFFLVEDLVDSLKLALLFYILTFVGAIFNGLTLLILGVIGLFTIPL LYRQHQAQIDQYVGLVTNQLSHIKAKIRAKIPGTGALASAAAAVSGSKAKAE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_206900 |
| ORF Size | 1416 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_206900.3 |
| RefSeq Size | 2100 bp |
| RefSeq ORF | 1419 bp |
| Locus ID | 6253 |
| UniProt ID | O75298 |
| Protein Families | Transmembrane |
| Gene Summary | This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. Reticulon proteins also play an important role in the replication of positive-strand RNA (ssRNA) viruses. Mutations at this locus have been associated with autosomal dominant spastic paraplegia-12. [provided by RefSeq, Aug 2020] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224942 | RTN2 (Myc-DDK-tagged)-Human reticulon 2 (RTN2), transcript variant 2 |
CNY 5,488.00 |
|
| RC224942L3 | Lenti-ORF clone of RTN2 (Myc-DDK-tagged)-Human reticulon 2 (RTN2), transcript variant 2 |
CNY 6,080.00 |
|
| RC224942L4 | Lenti-ORF clone of RTN2 (mGFP-tagged)-Human reticulon 2 (RTN2), transcript variant 2 |
CNY 6,080.00 |
|
| SC309827 | RTN2 (untagged)-Human reticulon 2 (RTN2), transcript variant 2 |
CNY 7,510.00 |
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