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KRIT1 (NM_194454) Human Tagged ORF Clone
CAT＃: RG224624
- TrueORF®
KRIT1 (tGFP-tagged) - Human KRIT1, ankyrin repeat containing (KRIT1), transcript variant 3
ORF Plasmid: DDK
"NM_194454" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CAM; CCM1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224624 representing NM_194454
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAATCCAGAAAACATAGAAGATGCATATGTTGCTGTTATTCGTCCAAAGAATACTGCCAGTCTCA ATTCTCGGGAATACAGAGCTAAGTCATATGAAATTTTGTTGCATGAAGTTCCCATTGAAGGACAGAAAAA AAAGAGAAAGAAAGTTTTATTGGAAACGAAACTTCAAGGCAACAGTGAAATAACACAAGGCATATTGGAT TACGTAGTAGAAACCACCAAACCAATTTCTCCTGCAAACCAGGGTATCAGAGGAAAACGAGTTGTACTAA TGAAAAAATTTCCTCTGGATGGAGAGAAGATGGGCAGAGAAGCATCATTATTTATTGTTCCATCAGTTGT CAAAGATAATACTAAATACACATATACCCCAGGATGCCCAATTTTTTACTGCTTACAAGATATTATGCGA GTCTGTAGTGAATCCAGTACTCATTTTGCTACACTTACAGCAAGGATGTTAATAGCCTTGGATAAGTGGT TAGATGAACGTCATGCACAATCTCACTTTATTCCAGCTTTATTCCGACCTTCTCCTCTTGAGCGGATAAA AACTAATGTCATAAATCCTGCATATGCTACTGAATCAGGTCAGACAGAAAACTCACTACATATGGGCTAT AGTGCACTAGAAATAAAGAGTAAAATGTTAGCCCTAGAGAAAGCAGATACCTGTATTTACAACCCTTTGT TTGGATCAGATCTTCAGTATACAAATCGGGTAGATAAAGTGGTAATAAATCCATACTTTGGTCTAGGAGC TCCAGACTACTCAAAAATCCAAATACCTAAACAGGAAAAATGGCAGAGAAGCATGAGCAGTGTCACAGAA GACAAGGAACGACAGTGGGTAGATGATTTTCCTCTCCACCGAAGCGCCTGTGAAGGAGATTCAGAATTAC TAAGCCGTCTTCTCAGTGAAAGATTTTCAGTCAACCAGTTAGATAGTGACCACTGGGCACCCATTCATTA TGCATGCTGGTATGGAAAAGTTGAGGCCACTCGCATATTGTTAGAGAAAGGAAAGTGCAATCCAAACCTT TTAAATGGACAACTTAGTTCTCCTCTTCATTTTGCTGCTGGAGGAGGACATGCTGAAATAGTACAGATTC TCCTAAACCACCCAGAAACGGATAGACATATAACAGACCAACAAGGAAGATCTCCATTAAATATTTGTGA AGAAAACAAACAAAACAACTGGGAAGAAGCTGCAAAATTGTTGAAGGAAGCAATTAACAAACCATATGAA AAAGTTCGAATATACAGAATGGATGGGTCATATCGTTCTGTTGAATTGAAGCATGGAAATAATACCACAG TGCAGCAGATAATGGAAGGAATGCGTCTCTCTCAAGAAACTCAGCAATATTTCACTATATGGATTTGTTC AGAAAACCTCAGCCTTCAACTCAAACCATATCATAAACCCTTGCAACATGTTCGTGACTGGCCAGAAATA CTTGCTGAATTGACTAATCTGGATCCTCAAAGGGAAACACCTCAGCTTTTTCTAAGAAGAGATGTGAGAC TTCCCTTGGAAGTTGAAAAACAGATTGAAGACCCACTAGCTATTCTTATTCTCTTTGATGAAGCCAGATA TAATTTATTGAAGGGCTTTTATACAGCTCCTGATGCTAAGCTGATAACATTGGCAAGTCTGCTTTTGCAA ATAGTCTATGGAAATTATGAGAGTAAAAAACACAAGCAAGGTTTCCTAAATGAAGAAAATCTAAAATCCA TCGTACCTGTTACCAAACTGAAAAGTAAGGCACCTCACTGGACAAATCGCATACTTCATGAATACAAGAA TCTCAGTACAAGTGAAGGTGTCAGTAAAGAAATGCATCACCTTCAGCGCATGTTCTTACAGAATTGCTGG GAAATTCCTACTTATGGAGCAGCATTTTTCACAGGACAGATATTTACAAAGGCAAGCCCCAGCAATCATA AAGTCATCCCTGTGTATGTAGGAGTGAATATAAAAGGACTTCATCTCCTCAACATGGAAACTAAGGCTTT ACTCATCAGTCTTAAGTATGGTTGTTTTATGTGGCAATTGGGAGATACTGATACTTGTTTTCAGATCCAT AGCATGGAAAATAAAATGAGCTTTATAGTACATACAAAACAGGCTGGTCTCGTGGTAAAACTGTTAATGA AGCTAAATGGACAGTTAATGCCCACTGAAAGAAATTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224624 representing NM_194454
Red=Cloning site Green=Tags(s) MGNPENIEDAYVAVIRPKNTASLNSREYRAKSYEILLHEVPIEGQKKKRKKVLLETKLQGNSEITQGILD YVVETTKPISPANQGIRGKRVVLMKKFPLDGEKMGREASLFIVPSVVKDNTKYTYTPGCPIFYCLQDIMR VCSESSTHFATLTARMLIALDKWLDERHAQSHFIPALFRPSPLERIKTNVINPAYATESGQTENSLHMGY SALEIKSKMLALEKADTCIYNPLFGSDLQYTNRVDKVVINPYFGLGAPDYSKIQIPKQEKWQRSMSSVTE DKERQWVDDFPLHRSACEGDSELLSRLLSERFSVNQLDSDHWAPIHYACWYGKVEATRILLEKGKCNPNL LNGQLSSPLHFAAGGGHAEIVQILLNHPETDRHITDQQGRSPLNICEENKQNNWEEAAKLLKEAINKPYE KVRIYRMDGSYRSVELKHGNNTTVQQIMEGMRLSQETQQYFTIWICSENLSLQLKPYHKPLQHVRDWPEI LAELTNLDPQRETPQLFLRRDVRLPLEVEKQIEDPLAILILFDEARYNLLKGFYTAPDAKLITLASLLLQ IVYGNYESKKHKQGFLNEENLKSIVPVTKLKSKAPHWTNRILHEYKNLSTSEGVSKEMHHLQRMFLQNCW EIPTYGAAFFTGQIFTKASPSNHKVIPVYVGVNIKGLHLLNMETKALLISLKYGCFMWQLGDTDTCFQIH SMENKMSFIVHTKQAGLVVKLLMKLNGQLMPTERNS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_194454 |
| ORF Size | 2208 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_194454.3 |
| RefSeq Size | 4523 bp |
| RefSeq ORF | 2211 bp |
| Locus ID | 889 |
| UniProt ID | O00522 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a protein containing four ankyrin repeats, a band 4.1/ezrin/radixin/moesin (FERM) domain, and multiple NPXY sequences. The encoded protein is localized in the nucleus and cytoplasm. It binds to integrin cytoplasmic domain-associated protein-1 alpha (ICAP1alpha), and plays a critical role in beta1-integrin-mediated cell proliferation. It associates with junction proteins and RAS-related protein 1A (Rap1A), which requires the encoded protein for maintaining the integrity of endothelial junctions. It is also a microtubule-associated protein and may play a role in microtubule targeting. Mutations in this gene result in cerebral cavernous malformations. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224624 | KRIT1 (Myc-DDK-tagged)-Human KRIT1, ankyrin repeat containing (KRIT1), transcript variant 3 |
CNY 5,392.00 |
|
| RC224624L3 | Lenti-ORF clone of KRIT1 (Myc-DDK-tagged)-Human KRIT1, ankyrin repeat containing (KRIT1), transcript variant 3 |
CNY 5,890.00 |
|
| RC224624L4 | Lenti-ORF clone of KRIT1 (mGFP-tagged)-Human KRIT1, ankyrin repeat containing (KRIT1), transcript variant 3 |
CNY 5,890.00 |
|
| SC107667 | KRIT1 (untagged)-Human KRIT1, ankyrin repeat containing (KRIT1), transcript variant 3 |
CNY 5,400.00 |
