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MFI2 (MELTF) (NM_005929) Human Tagged ORF Clone
CAT＃: RG224600
- TrueORF®
MFI2 (tGFP-tagged) - Human antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 (MFI2), transcript variant 1
ORF Plasmid: DDK
"NM_005929" in other vectors (6)
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CNY 7,008.00
CNY 8,460.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CD228; MAP97; MFI2; MTf; MTF1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224600 representing NM_005929
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGTCCGAGCGGGGCTCTGTGGCTGCTCCTGGCTCTGCGCACCGTGCTCGGAGGCATGGAGGTGC GGTGGTGCGCCACCTCGGACCCAGAGCAGCACAAGTGCGGCAACATGAGCGAGGCCTTCCGGGAAGCGGG CATCCAGCCCTCCCTCCTCTGCGTCCGGGGCACCTCCGCCGACCACTGCGTCCAGCTCATCGCGGCCCAG GAGGCTGACGCCATCACTCTGGATGGAGGAGCCATCTATGAGGCGGGAAAGGAGCACGGCCTGAAGCCGG TGGTGGGCGAAGTGTACGATCAAGAGGTCGGTACCTCCTATTACGCCGTGGCTGTGGTCAGGAGGAGCTC CCATGTGACCATTGACACCCTGAAAGGCGTGAAGTCCTGCCACACGGGCATCAATCGCACAGTGGGCTGG AACGTGCCCGTGGGCTACCTGGTGGAGAGCGGCCGCCTCTCGGTGATGGGCTGCGATGTACTCAAAGCTG TCAGCGACTATTTTGGGGGCAGCTGCGTCCCGGGGGCAGGAGAGACCAGTTACTCTGAGTCCCTCTGTCG CCTCTGCAGGGGTGACAGCTCTGGGGAAGGGGTGTGTGACAAGAGCCCCCTGGAGAGATACTACGACTAC AGCGGGGCCTTCCGGTGCCTGGCGGAAGGGGCAGGGGACGTGGCTTTTGTGAAGCACAGCACGGTACTGG AGAACACGGATGGGAAGACGCTTCCCTCCTGGGGCCAGGCCCTGCTGTCACAGGACTTCGAGCTGCTGTG CCGGGATGGTAGCCGGGCCGATGTCACCGAGTGGAGGCAGTGCCATCTGGCCCGGGTGCCTGCTCACGCC GTGGTGGTCCGGGCCGACACAGATGGGGGCCTCATCTTCCGGCTGCTCAACGAAGGCCAGCGTCTGTTCA GCCACGAGGGCAGCAGCTTCCAGATGTTCAGCTCTGAGGCCTATGGCCAGAAGGATCTACTCTTCAAAGA CTCTACCTCGGAGCTTGTGCCCATCGCCACACAGACCTATGAGGCGTGGCTGGGCCATGAGTACCTGCAC GCCATGAAGGGTCTGCTCTGTGACCCCAACCGGCTGCCCCCCTACCTGCGCTGGTGTGTGCTCTCCACTC CCGAGATCCAGAAGTGTGGAGACATGGCCGTGGCCTTCCGCCGGCAGCGCCTCAAGCCAGAGATCCAGTG CGTGTCAGCCAAGTCCCCCCAACACTGCATGGAGCGGATCCAGGCTGAGCAGGTCGACGCTGTGACCCTA AGTGGCGAGGACATTTACACGGCGGGGAAGAAGTACGGCCTGGTTCCCGCAGCCGGCGAGCACTATGCCC CGGAAGACAGCAGCAACTCGTACTACGTGGTGGCCGTGGTGAGACGGGACAGCTCCCACGCCTTCACCTT GGATGAGCTTCGGGGCAAGCGCTCCTGCCACGCCGGTTTCGGCAGCCCTGCAGGCTGGGATGTCCCCGTG GGTGCCCTTATTCAGAGAGGCTTCATCCGGCCCAAGGACTGTGACGTCCTCACAGCAGTGAGCGAGTTCT TCAATGCCAGCTGCGTGCCCGTGAACAACCCCAAGAACTACCCCTCCTCGCTGTGTGCACTGTGCGTGGG GGACGAGCAGGGCCGCAACAAGTGTGTGGGCAACAGCCAGGAGCGGTATTACGGCTACCGCGGCGCCTTC AGGTGCCTGGTGGAGAATGCGGGTGACGTTGCCTTCGTCAGGCACACAACCGTCTTTGACAACACAAACG GCCACAATTCCGAGCCCTGGGCTGCTGAGCTCAGGTCAGAGGACTATGAACTGCTGTGCCCCAACGGGGC CCGAGCCGAGGTGTCCCAGTTTGCAGCCTGCAACCTGGCACAGATACCACCCCACGCCGTGATGGTCCGG CCCGACACCAACATCTTCACCGTGTATGGACTGCTGGACAAGGCCCAGGACCTGTTTGGAGACGACCACA ATAAGAACGGGTTCAAAATGTTCGACTCCTCCAACTATCATGGCCAAGACCTGCTTTTCAAGGATGCCAC CGTCCGGGCGGTGCCTGTCGGAGAGAAAACCACCTACCGCGGCTGGCTGGGGCTGGACTACGTGGCGGCG CTGGAAGGGATGTCGTCTCAGCAGTGCTCGGGCGCAGCGGCCCCGGCGCCCGGGGCGCCCCTGCTCCCGC TGCTGCTGCCCGCCCTCGCCGCCCGCCTGCTCCCGCCCGCCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224600 representing NM_005929
Red=Cloning site Green=Tags(s) MRGPSGALWLLLALRTVLGGMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQ EADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGW NVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDY SGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHA VVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLH AMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTL SGEDIYTAGKKYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPV GALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAF RCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVR PDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAA LEGMSSQQCSGAAAPAPGAPLLPLLLPALAARLLPPAL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005929 |
| ORF Size | 2214 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005929.3, NP_005920.1 |
| RefSeq Size | 2377 bp |
| RefSeq ORF | 2217 bp |
| Locus ID | 4241 |
| UniProt ID | P08582 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224600 | MFI2 (Myc-DDK-tagged)-Human antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 (MFI2), transcript variant 1 |
CNY 5,408.00 |
|
| RC224600L1 | Lenti ORF clone of Human antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 (MFI2), transcript variant 1, Myc-DDK-tagged |
CNY 7,808.00 |
|
| RC224600L2 | Lenti ORF clone of Human antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 (MFI2), transcript variant 1, mGFP tagged |
CNY 9,600.00 |
|
| RC224600L3 | Lenti ORF clone of Human antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 (MFI2), transcript variant 1, Myc-DDK-tagged |
CNY 9,600.00 |
|
| RC224600L4 | Lenti ORF clone of Human antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 (MFI2), transcript variant 1, mGFP tagged |
CNY 9,600.00 |
|
| SC303705 | MFI2 (untagged)-Human antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 (MFI2), transcript variant 1 |
CNY 5,952.00 |
