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SCAP (NM_012235) Human Tagged ORF Clone
CAT＃: RG223734
- TrueORF®
SCAP (tGFP-tagged) - Human SREBF chaperone (SCAP)
ORF Plasmid: DDK
"NM_012235" in other vectors (6)
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CNY 10,504.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223734 representing NM_012235
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTGACTGAAAGGCTGCGTGAGAAGATATCTCGGGCCTTCTACAACCATGGGCTCCTCTGTGCAT CCTATCCCATCCCCATCATCCTCTTCACAGGGTTCTGCATCTTAGCCTGCTGCTACCCACTGCTGAAACT CCCCTTGCCAGGAACAGGACCTGTGGAATTCACCACCCCTGTGAAGGATTACTCGCCCCCACCTGTGGAC TCTGACCGCAAACAAGGAGAGCCTACTGAGCAGCCTGAGTGGTATGTGGGTGCCCCGGTGGCTTATGTCC AGCAGATATTTGTGAAGTCCTCAGTGTTTCCCTGGCACAAGAACCTCCTGGCAGTAGATGTATTTCGTTC ACCTTTGTCCCGGGCATTCCAACTGGTGGAGGAGATCCGGAACCACGTGCTGAGAGACAGCTCTGGGATC AGGAGCTTGGAGGAGTTGTGTCTGCAAGTGACCGACCTGCTGCCAGGCCTTAGGAAGCTCAGGAACCTAC TCCCTGAGCATGGATGCCTGCTGCTGTCCCCTGGGAACTTCTGGCAGAATGACTGGGAACGCTTCCATGC TGATCCTGACATCATTGGGACCATCCACCAGCACGAGCCTAAAACCCTGCAGACTTCAGCCACACTCAAA GACTTGTTATTTGGTGTTCCTGGGAAGTACAGCGGGGTGAGCCTCTACACCAGGAAGAGGATGGTCTCCT ACACCATCACCCTGGTCTTCCAGCACTACCATGCCAAGTTCCTGGGCAGCCTGCGTGCCCGCCTGATGCT TCTGCACCCCAGCCCCAACTGCAGCCTTCGGGCGGAGAGCCTGGTCCACGTGCACTTCAAGGAGGAGATT GGTGTCGCTGAGCTCATCCCCCTTGTGACCACCTACATCATCTTGTTTGCCTACATCTACTTCTCCACGC GGAAGATCGACATGGTCAAGTCCAAGTGGGGGCTGGCCCTGGCTGCCGTGGTCACAGTGCTCAGCTCGCT GCTCATGTCTGTGGGACTCTGCACACTCTTCGGCCTGACGCCCACCCTCAATGGCGGCGAGATTTTCCCC TACCTTGTGGTGGTTATTGGGTTAGAGAATGTGTTGGTGCTCACCAAGTCTGTGGTCTCAACCCCGGTAG ACCTGGAGGTGAAGCTGCGGATCGCCCAAGGCCTAAGCAGCGAGAGCTGGTCCATCATGAAGAACATGGC CACGGAGCTGGGCATCATCCTCATCGGCTACTTCACCCTAGTGCCCGCCATCCAGGAGTTCTGTCTCTTT GCTGTCGTGGGGCTGGTGTCTGACTTCTTCCTTCAGATGCTGTTTTTCACCACTGTCCTGTCCATTGACA TTCGCCGGATGGAGCTAGCAGACCTGAACAAGCGACTGCCCCCTGAGGCCTGCCTGCCCTCAGCCAAGCC AGTGGGACAGCCAACGCGCTACGAGCGGCAGCTGGCTGTGAGGCCGTCCACACCCCACACCATCACGTTG CAGCCGTCTTCCTTCCGAAACCTGCGGCTCCCCAAGAGGCTGCGTGTTGTCTACTTCCTGGCCCGCACCC GCCTGGCACAGCGCCTCATCATGGCTGGCACCGTTGTCTGGATTGGCATCCTGGTATACACAGACCCAGC AGGGCTGCGCAACTACCTCGCTGCCCAGGTGACGGAACAGAGCCCATTGGGTGAGGGAGCCCTGGCTCCC ATGCCCGTGCCTAGTGGCATGCTGCCCCCCAGCCACCCGGACCCTGCCTTCTCCATCTTCCCACCTGATG CCCCTAAGCTACCTGAGAACCAGACGTCGCCAGGCGAGTCACCTGAGCGTGGAGGTCCAGCAGAGGTTGT CCATGACAGCCCAGTCCCAGAGGTAACCTGGGGGCCTGAGGATGAGGAACTTTGGAGGAAATTGTCCTTC CGCCACTGGCCGACGCTCTTCAGCTATTACAACATCACACTGGCCAAGAGGTACATCAGCCTGCTGCCCG TCATCCCAGTCACGCTCCGCCTGAACCCGAGGGAGGCTCTGGAGGGCCGGCACCCTCAGGACGGCCGCAG TGCCTGGCCCCCACCGGGGCCCATACCTGCTGGGCACTGGGAAGCAGGACCCAAGGGCCCAGGTGGGGTG CAGGCCCATGGAGACGTCACGCTGTACAAGGTGGCGGCGCTGGGCCTGGCCACCGGCATCGTCTTGGTGC TGCTGCTGCTCTGCCTCTACCGCGTGCTATGCCCGCGCAACTACGGGCAGCTGGGTGGTGGGCCCGGGCG GCGGAGGCGCGGGGAGCTGCCCTGCGACGACTACGGCTATGCGCCACCCGAGACGGAGATCGTGCCGCTT GTGCTGCGCGGCCACCTCATGGACATCGAGTGCCTGGCCAGCGACGGCATGCTGCTGGTGAGCTGCTGCC TGGCAGGCCACGTCTGCGTGTGGGACGCGCAGACCGGGGATTGCCTAACGCGCATTCCGCGCCCAGGCAG GCAGCGCCGGGACAGTGGCGTGGGCAGCGGGCTTGAGGCTCAGGAGAGCTGGGAACGACTTTCAGATGGT GGGAAGGCTGGTCCAGAGGAGCCTGGGGACAGCCCTCCCCTGAGACACCGCCCCCGGGGCCCTCCGCCGC CTTCCCTCTTCGGGGACCAGCCTGACCTCACCTGCTTAATTGACACCAACTTTTCAGCGCAGCCTCGGTC CTCACAGCCCACTCAGCCCGAGCCCCGGCACCGGGCGGTCTGTGGCCGCTCTCGGGACTCCCCAGGCTAT GACTTCAGCTGCCTGGTGCAGCGGGTGTACCAGGAGGAGGGGCTGGCGGCCGTCTGCACACCAGCCCTGC GCCCACCCTCGCCTGGGCCGGTGCTGTCCCAGGCCCCTGAGGACGAGGGTGGCTCCCCCGAGAAAGGCTC CCCTTCCCTCGCCTGGGCCCCCAGTGCCGAGGGTTCCATCTGGAGCTTGGAGCTGCAGGGCAACCTCATC GTGGTGGGGCGGAGCAGCGGCCGGCTGGAGGTGTGGGACGCCATTGAAGGGGTGCTGTGCTGCAGCAGCG AGGAGGTCTCCTCAGGCATTACCGCTCTGGTGTTCTTGGACAAAAGGATTGTGGCTGCACGGCTCAACGG TTCCCTTGATTTCTTCTCCTTGGAGACCCACACTGCCCTCAGCCCCCTGCAGTTTAGAGGGACCCCAGGG CGGGGCAGTTCCCCTGCCTCTCCAGTGTACAGCAGCAGCGACACAGTGGCCTGTCACCTGACCCACACAG TGCCCTGTGCACACCAAAAACCCATCACAGCCCTGAAAGCCGCTGCTGGGCGCTTGGTGACTGGGAGCCA AGACCACACACTGAGAGTGTTCCGTCTGGAGGACTCGTGCTGCCTCTTCACCCTTCAGGGCCACTCAGGG GCCATCACGACCGTGTACATTGACCAGACCATGGTGCTGGCCAGTGGAGGACAAGATGGGGCCATCTGCC TGTGGGATGTACTGACTGGCAGCCGGGTCAGCCATGTGTTTGCTCACCGTGGGGATGTCACCTCCCTTAC CTGTACCACCTCCTGTGTCATCAGCAGTGGCCTGGATGACCTCATCAGCATCTGGGACCGCAGCACAGGC ATCAAGTTCTACTCCATTCAGCAGGACCTGGGCTGTGGTGCAAGCTTGGGTGTCATCTCAGACAACCTGC TGGTGACTGGCGGCCAGGGCTGTGTCTCCTTTTGGGACCTAAACTACGGGGACCTGTTACAGACAGTCTA CCTGGGGAAGAACAGTGAGGCCCAGCCTGCCCGCCAGATCCTGGTGCTGGACAACGCTGCCATTGTCTGC AACTTTGGCAGTGAGCTCAGCCTGGTGTATGTGCCCTCTGTGCTGGAGAAGCTGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223734 representing NM_012235
Red=Cloning site Green=Tags(s) MTLTERLREKISRAFYNHGLLCASYPIPIILFTGFCILACCYPLLKLPLPGTGPVEFTTPVKDYSPPPVD SDRKQGEPTEQPEWYVGAPVAYVQQIFVKSSVFPWHKNLLAVDVFRSPLSRAFQLVEEIRNHVLRDSSGI RSLEELCLQVTDLLPGLRKLRNLLPEHGCLLLSPGNFWQNDWERFHADPDIIGTIHQHEPKTLQTSATLK DLLFGVPGKYSGVSLYTRKRMVSYTITLVFQHYHAKFLGSLRARLMLLHPSPNCSLRAESLVHVHFKEEI GVAELIPLVTTYIILFAYIYFSTRKIDMVKSKWGLALAAVVTVLSSLLMSVGLCTLFGLTPTLNGGEIFP YLVVVIGLENVLVLTKSVVSTPVDLEVKLRIAQGLSSESWSIMKNMATELGIILIGYFTLVPAIQEFCLF AVVGLVSDFFLQMLFFTTVLSIDIRRMELADLNKRLPPEACLPSAKPVGQPTRYERQLAVRPSTPHTITL QPSSFRNLRLPKRLRVVYFLARTRLAQRLIMAGTVVWIGILVYTDPAGLRNYLAAQVTEQSPLGEGALAP MPVPSGMLPPSHPDPAFSIFPPDAPKLPENQTSPGESPERGGPAEVVHDSPVPEVTWGPEDEELWRKLSF RHWPTLFSYYNITLAKRYISLLPVIPVTLRLNPREALEGRHPQDGRSAWPPPGPIPAGHWEAGPKGPGGV QAHGDVTLYKVAALGLATGIVLVLLLLCLYRVLCPRNYGQLGGGPGRRRRGELPCDDYGYAPPETEIVPL VLRGHLMDIECLASDGMLLVSCCLAGHVCVWDAQTGDCLTRIPRPGRQRRDSGVGSGLEAQESWERLSDG GKAGPEEPGDSPPLRHRPRGPPPPSLFGDQPDLTCLIDTNFSAQPRSSQPTQPEPRHRAVCGRSRDSPGY DFSCLVQRVYQEEGLAAVCTPALRPPSPGPVLSQAPEDEGGSPEKGSPSLAWAPSAEGSIWSLELQGNLI VVGRSSGRLEVWDAIEGVLCCSSEEVSSGITALVFLDKRIVAARLNGSLDFFSLETHTALSPLQFRGTPG RGSSPASPVYSSSDTVACHLTHTVPCAHQKPITALKAAAGRLVTGSQDHTLRVFRLEDSCCLFTLQGHSG AITTVYIDQTMVLASGGQDGAICLWDVLTGSRVSHVFAHRGDVTSLTCTTSCVISSGLDDLISIWDRSTG IKFYSIQQDLGCGASLGVISDNLLVTGGQGCVSFWDLNYGDLLQTVYLGKNSEAQPARQILVLDNAAIVC NFGSELSLVYVPSVLEKLD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012235 |
| ORF Size | 3837 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012235.4 |
| RefSeq Size | 4255 bp |
| RefSeq ORF | 3840 bp |
| Locus ID | 22937 |
| UniProt ID | Q12770 |
| Domains | WD40 |
| Protein Families | Druggable Genome, Transcription Factors, Transmembrane |
| Gene Summary | This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223734 | SCAP (Myc-DDK-tagged)-Human SREBF chaperone (SCAP) |
CNY 8,904.00 |
|
| RC223734L1 | Lenti ORF clone of Human SREBF chaperone (SCAP), Myc-DDK-tagged |
CNY 11,304.00 |
|
| RC223734L2 | Lenti ORF clone of Human SREBF chaperone (SCAP), mGFP tagged |
CNY 11,500.00 |
|
| RC223734L3 | Lenti ORF clone of Human SREBF chaperone (SCAP), Myc-DDK-tagged |
CNY 11,304.00 |
|
| RC223734L4 | Lenti ORF clone of Human SREBF chaperone (SCAP), mGFP tagged |
CNY 11,304.00 |
|
| SC315330 | SCAP (untagged)-Human SREBF chaperone (SCAP) |
CNY 8,912.00 |
