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SPG7 (NM_199367) Human Tagged ORF Clone
CAT＃: RG223633
- TrueORF®
SPG7 (tGFP-tagged) - Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 2. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_199367" in other vectors (4)
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CNY 4,750.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CAR; CMAR; PGN; SPG5C |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223633 ORF sequence, codon optimized.
Due to the complexity of NM_199367, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGTTCTTCTGCTCCTTCTGAGAGCATTGCGCAGGGGCCCCGGACCTGGTCCCCGACCATTGTGGG GACCAGGTCCAGCTTGGAGCCCTGGTTTTCCCGCTCGCCCTGGGAGGGGTAGACCCTACATGGCCTCCCG CCCCCCGGGAGACTTGGCAGAAGCCGGTGGCAGGGCATTGCAAAGTCTCCAGCTGAGACTGCTGACACCA ACATTCGAGGGAATCAATGGTCTTCTGCTGAAGCAGCACCTGGTCCAGAATCCGGTGCGGCTGTGGCAGC TCCTCGGGGGGACCTTTTATTTTAATACCAGCCGGCTGAAACAAAAGAATAAAGAGAAGGACAAGTCCAA AGGGAAGGCCCCAGAAGAGGACGAAGAAGAACGGCGCCGCAGAGAGAGGGATGATCAGATGTACAGGGAA AGACTGCGGACCCTGTTGGTAATTGCGGTGGTTATGTCACTCCTGAACGCCTTGTCCACCAGCGGCGGAT CTATCTCCTGGAACGATTTCGTGCATGAAATGCTTGCTAAAGGGGAGGTGCAGCGGGTGCAGGTAGTTCC TGAGTCCGACGTGGTGGAGGTTTATCTGCACCCCGGAGCGGTGGTGTTCGGAAGACCTCGGCTGGCCCTG ATGTACAGAATGCAAGTGGCTAACATTGATAAGTTCGAGGAGAAGCTGCGGGCTGCTGAGGACGAACTTA ATATTGAGGCCAAAGACAGGATACCCGTCAGTTACAAGAGGACCGGCTTCTTTGGCAATGCGCTGTACTC AGTGGGCATGACGGCCGTGGGACTGGCCATTTTGTGGTACGTGTTTCGGCTTGCTGGAATGACAGGAAGG GAAGGTGGCTTTTCAGCTTTCAATCAGCTGAAGATGGCACGATTCACCATCGTGGATGGCAAGATGGGCA AAGGAGTGAGCTTTAAGGACGTGGCTGGCATGCATGAAGCCAAGCTGGAAGTGCGGGAATTCGTGGACTA TCTGAAATCCCCGGAAAGATTTCTCCAGCTGGGTGCCAAGGTTCCTAAGGGCGCCCTGCTGCTCGGGCCA CCAGGATGTGGCAAAACGCTGCTGGCAAAAGCCGTGGCCACGGAGGCACAAGTGCCCTTTCTGGCAATGG CGGGCCCTGAATTTGTTGAAGTGATTGGCGGCCTCGGAGCAGCCCGGGTTAGAAGTTTGTTCAAGGAAGC ACGAGCTCGGGCTCCATGCATCGTCTACATCGACGAGATCGACGCAGTGGGAAAAAAACGAAGCACCACC ATGAGTGGCTTTTCTAATACCGAGGAGGAACAGACACTGAACCAGCTGCTGGTTGAGATGGACGGTGCCA GCCTTGATCAACTGCCCTCCCAAGGTACCATGAGGAAGCTCAGGGGAAAGACTCCAGCCTGTTCTTGCCT GACCGAACCCACCGGCTCAAGAAGAGCCATGGAGGGACACAGTCTGTGTTGGGGATGCCTCTTGCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223633 representing NM_199367
Red=Cloning site Green=Tags(s) MAVLLLLLRALRRGPGPGPRPLWGPGPAWSPGFPARPGRGRPYMASRPPGDLAEAGGRALQSLQLRLLTP TFEGINGLLLKQHLVQNPVRLWQLLGGTFYFNTSRLKQKNKEKDKSKGKAPEEDEEERRRRERDDQMYRE RLRTLLVIAVVMSLLNALSTSGGSISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLAL MYRMQVANIDKFEEKLRAAEDELNIEAKDRIPVSYKRTGFFGNALYSVGMTAVGLAILWYVFRLAGMTGR EGGFSAFNQLKMARFTIVDGKMGKGVSFKDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGP PGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTT MSGFSNTEEEQTLNQLLVEMDGASLDQLPSQGTMRKLRGKTPACSCLTEPTGSRRAMEGHSLCWGCLLH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_199367 |
| ORF Size | 1467 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_199367.1, NP_955399.1 |
| RefSeq Size | 2328 bp |
| RefSeq ORF | 1470 bp |
| Locus ID | 6687 |
| UniProt ID | Q9UQ90 |
| Protein Families | Protease, Transmembrane |
| Gene Summary | This gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223633 | SPG7 (Myc-DDK-tagged)-Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 2. Note: ORF is codon optimized |
CNY 3,656.00 |
|
| RC223633L3 | Lenti-ORF clone of SPG7 (Myc-DDK-tagged)-Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 2. Note: ORF is codon optimized |
CNY 6,180.00 |
|
| RC223633L4 | Lenti-ORF clone of SPG7 (mGFP-tagged)-Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 2. Note: ORF is codon optimized |
CNY 6,180.00 |
|
| SC307961 | SPG7 (untagged)-Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 7,890.00 |
