United States
Germany
Japan
United Kingdom
China
ZSWIM8 (NM_015037) Human Tagged ORF Clone
CAT＃: RG223577
- TrueORF®
ZSWIM8 (tGFP-tagged) - Human KIAA0913 (KIAA0913). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_015037" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 20,140.00
货期*
3周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | KIAA0913 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223577 ORF sequence, codon optimized.
Due to the complexity of NM_015037, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGATGTTCGCAGAGTGGGAGGATGGCGAAAGGTTCAGTTTTGAAGACTCAGACAGATTCGAGG AAGACTCCCTGTGCAGCTTTATCAGTGAAGCCGAATCCCTGTGCCAAAATTGGAGGGGGTGGAGAAAGCA GAGCGCTGGACCTAACTCCCCCACAGGCGGGGGCGGGGGAGGTGGCTCAGGCGGTACCAGAATGAGGGAT GGGCTGGTGATCCCACTGGTGGAGTTGTCCGCCAAGCAGGTGGCCTTTCACATACCTTTCGAAGTAGTGG AGAAAGTCTATCCCCCCGTTCCTGAACAGCTCCAGCTGCGCATTGCGTTCTGGAGCTTCCCCGAAAACGA GGAAGATATACGCCTCTATAGCTGTTTGGCGAACGGGTCAGCTGATGAATTTCAGCGGGGGGACCAATTG TTTAGAATGAGGGCCGTGAAGGATCCACTGCAGATCGGCTTCCACCTCAGCGCCACAGTGGTCCCGCCAC AAATGGTCCCTCCAAAAGGAGCATACAATGTGGCTGTTATGTTCGATCGGTGCAGGGTAACCAGTTGCTC TTGCACCTGCGGTGCCGGGGCAAAATGGTGTACTCACGTGGTGGCATTGTGTCTGTTCCGCATTCACAAT GCATCTGCCGTGTGCCTGAGGGCACCCGTGAGTGAGAGCCTGAGCAGACTGCAAAGAGATCAGTTGCAGA AATTCGCCCAGTATCTGATTAGTGAGCTCCCTCAGCAGATACTCCCTACTGCACAGCGGCTGCTGGACGA ACTGTTGTCTTCACAATCAACCGCCATCAATACCGTATGCGGGGCCCCCGATCCGACGGCGGGGCCTAGT GCTAGCGACCAATCTACTTGGTACCTGGATGAGTCCACCCTTACAGATAATATTAAAAAGACCCTGCACA AATTTTGCGGACCCAGCCCTGTCGTTTTCAGTGATGTAAATTCAATGTATCTGAGTAGTACTGAGCCCCC CGCCGCTGCCGAGTGGGCATGCCTGCTGAGGCCCCTTCGAGGTCGCGAGCCTGAGGGCGTTTGGAATCTC CTTTCCATTGTAAGGGAGATGTTTAAGAGGAGGGACAGCAATGCTGCACCCCTGTTGGAGATTCTGACCG ATCAGTGTCTGACATACGAACAGATTACTGGATGGTGGTATAGCGTGAGGACTTCAGCAAGTCACTCTAG TGCCAGTGGACATACTGGAAGATCAAATGGGCAATCCGAGGTCGCCGCCCATGCGTGTGCTAGTATGTGC GATGAAATGGTGACCCTCTGGAGGTTGGCTGTGCTTGATCCAGCTCTCAGCCCACAGCGACGCAGGGAGC TGTGCACACAACTGCGCCAGTGGCAGCTCAAAGTGATAGAAAATGTAAAGCGAGGCCAGCATAAGAAGAC GCTTGAGCGCCTCTTTCCAGGCTTCAGGCCTGCAGTGGAGGCGTGCTATTTTAATTGGGAGGAGGCTTAC CCATTGCCCGGAGTTACATACTCTGGAACAGACAGAAAGCTGGCTCTCTGCTGGGCCAGGGCGCTTCCCT CCCGGCCTGGCGCCTCCCGCAGTGGGGGCCTCGAGGAGAGCCGGGACCGACCTCGCCCCCTGCCCACTGA GCCCGCGGTGCGACCCAAGGAACCCGGCACCAAGAGGAAAGGCCTGGGAGAGGGGGTGCCATCCTCCCAG CGCGGTCCCAGGAGGCTGAGCGCGGAGGGAGGGGACAAGGCATTGCACAAGATGGGCCCAGGGGGCGGGA AGGCCAAGGCCCTCGGCGGGGCCGGCTCAGGGAGTAAGGGCTCCGCTGGAGGAGGGTCTAAGAGAAGACT CTCCTCCGAAGATAGCTCTCTGGAACCCGATCTGGCCGAGATGTCTCTGGATGACAGTAGTCTCGCTCTG GGTGCTGAGGCATCCACCTTCGGGGGGTTCCCCGAGTCCCCTCCACCCTGTCCTCTCCATGGAGGATCTA GGGGACCAAGCACATTTCTGCCCGAACCACCGGACACCTACGAGGAGGACGGTGGAGTGTATTTTTCAGA AGGTCCCGAACCTCCCACCGCATCAGTTGGTCCCCCTGGACTGCTGCCTGGCGACGTCTGTACCCAAGAT GATCTGCCGTCAACCGACGAGAGCGGTAACGGCCTGCCAAAAACAAAGGAGGCGGCTCCCGCGGTCGGTG AGGAGGATGATGATTATCAGGCGTATTACCTTAATGCACAGGACGGAGCCGGAGGAGAGGAGGAGAAAGC AGAAGGCGGGGCTGGCGAGGAGCACGACCTCTTCGCCGGCCTGAAGCCGCTTGAACAAGAATCTAGGATG GAAGTTCTGTTCGCTTGCGCTGAGGCATTGCATGCCCATGGGTATTCATCTGAAGCCAGTCGGTTGACTG TGGAGCTCGCGCAGGATCTGCTCGCCAATCCCCCAGATCTGAAAGTGGAACCTCCTCCAGCCAAGGGCAA GAAAAACAAAGTCTCAACCTCCAGACAAACCTGGGTGGCGACAAACACACTCTCCAAAGCAGCCTTCCTG CTTACTGTGCTGTCTGAGCGGCCTGAACATCATAACTTGGCCTTCAGGGTTGGCATGTTCGCCCTTGAAC TGCAGAGGCCTCCAGCCAGCACCAAGGCATTGGAAGTCAAACTGGCGTATCAGGAGAGCGAGGTCGCTGC CTTGCTGAAGAAGATACCGCTGGGGCCATCAGAGATGTCCACCATGAGATGTCGGGCTGAAGAGCTCAGG GAGGGGACTTTGTGTGATTATCGACCTGTGCTGCCTCTGATGCTCGCATCTTTCATTTTCGACGTTCTGT GCGCTCCCGTGGTCAGCCCAACAGGAAGCCGACCTCCAAGCAGGAACTGGAACTCAGAGACTCCCGGCGA TGAGGAGCTTGGATTCGAAGCGGCCGTAGCCGCACTCGGTATGAAAACCACAGTCAGCGAGGCCGAGCAT CCTCTGTTGTGCGAGGGGACCCGGAGAGAGAAAGGGGATCTTGCCCTGGCTCTCATGATTACATATAAGG ATGACCAGGCGAAACTGAAAAAGATTCTGGATAAGCTGCTGGACAGGGAGTCCCAGACCCACAAACCCCA GACCCTCTCAAGCTTCTATTCCAGCAGTCGCCCCACAACCGCGAGCCAGCGGTCACCAAGCAAACACGGA GGCCCATCTGCTCCGGGAGCTTTGCAACCCTTGACGAGCGGAAGCGCTGGACCTGCCCAGCCCGGTAGCG TTGCTGGCGCTGGCCCTGGCCCCACAGAAGGCTTCACGGAGAAGAACGTGCCCGAGAGCAGCCCCCACAG TCCTTGCGAAGGCCTTCCAAGCGAGGCGGCCCTTACTCCTAGACCAGAGGGCAAGGTCCCCTCCCGGCTC GCACTGGGCTCCAGGGGCGGTTACAACGGGAGGGGGTGGGGGAGCCCCGGAAGACCAAAGAAGAAGCACA CAGGTATGGCATCAATTGACAGTAGTGCTCCTGAAACTACCAGTGATTCATCTCCCACCCTCTCCAGAAG ACCGCTCCGCGGCGGCTGGGCTCCTACTAGCTGGGGCAGAGGACAAGATTCAGATAGCATCTCCTCATCA TCTTCCGACAGCCTCGGCAGCTCCTCCAGTAGTGGGAGCAGGCGGGCCAGCGCATCTGGCGGGGCCAGAG CCAAGACTGTGGAGGTGGGTCGCTACAAGGGCCGCCGACCTGAGTCCCACGCACCCCACGTGCCCAACCA GCCTTCTGAAGCCGCCGCCCATTTCTATTTCGAACTTGCCAAGACCGTTCTGATCAAGGCCGGCGGTAAC TCTTCTACATCCATTTTCACCCATCCAAGCTCATCCGGTGGACACCAAGGGCCACACCGGAACCTGCACC TGTGTGCCTTTGAAATTGGTCTGTACGCCTTGGGCCTGCATAACTTTGTGAGCCCAAATTGGCTTAGCAG GACCTACTCTTCACACGTATCATGGATCACGGGCCAGGCTATGGAGATCGGAAGCGCTGCTCTCACAATC CTCGTCGAGTGCTGGGATGGCCACCTCACACCGCCAGAGGTGGCTTCACTCGCTGATCGGGCTTCTCGGG CACGAGACTCCAATATGGTGCGCGCAGCTGCCGAGCTCGCCCTTTCCTGTCTGCCGCACGCACATGCTCT CAATCCTAATGAGATCCAACGGGCATTGGTCCAGTGCAAAGAGCAGGACAACCTCATGCTCGAAAAAGCC TGTATGGCTGTTGAAGAAGCCGCGAAAGGCGGCGGTGTATACCCAGAGGTACTCTTCGAGGTGGCACATC AGTGGTTTTGGCTGTATGAACAGACTGCCGGAGGCAGCTCCACAGCCCGCGAGGGAGCTACATCATGTTC AGCTAGCGGGATCAGAGCTGGAGGAGAGGCCGGGCGGGGTATGCCAGAAGGAAGGGGTGGCCCAGGCACC GAACCCGTGACTGTCGCCGCTGCGGCTGTGACTGCCGCTGCTACAGTCGTGCCCGTGATCTCTGTGGGCT CTTCACTCTACCCTGGGCCCGGACTGGGGCACGGACACTCTCCGGGCCTGCATCCTTACACAGCCCTCCA GCCACACTTGCCCTGTAGTCCACAATATCTCACCCACCCTGCACACCCCGCCCACCCAATGCCCCACATG CCCCGGCCCGCTGTGTTTCCCGTGCCTTCATCCGCATACCCACAGGGCGTGCACCCAGCCTTTCTTGGAG CACAGTATCCATATTCAGTGACGCCCCCAAGTCTGGCGGCAACAGCCGTGTCCTTTCCAGTGCCTTCAAT GGCTCCAATTACCGTTCACCCTTACCACACTGAGCCTGGGTTGCCCCTGCCCACAAGTGTTGCTTGTGAG CTCTGGGGACAGGGTACAGTGAGCAGTGTTCATCCTGCATCTACCTTTCCCGCCATCCAAGGGGCCTCAC TCCCCGCCCTGACTACACAACCTAGTCCTCTTGTTAGCGGAGGTTTCCCCCCCCCAGAGGAGGAGACACA CAGTCAGCCAGTTAACCCCCATAGTCTTCACCACCTCCACGCCGCTTATCGAGTGGGGATGCTCGCCTTG GAAATGCTGGGACGAAGAGCTCACAACGACCACCCAAACAACTTCTCTCGGAGCCCTCCCTACACTGATG ACGTGAAGTGGCTGCTGGGCTTGGCTGCGAAGCTGGGCGTGAATTATGTACACCAGTTCTGTGTGGGCGC GGCAAAAGGCGTGTTGTCTCCTTTTGTTCTGCAGGAAATCGTCATGGAAACACTGCAGCGACTTTCACCG GCTCACGCCCATAACCACTTGAGAGCGCCGGCATTCCATCAGCTTGTGCAGCGATGCCAACAGGCGTATA TGCAGTACATACACCACAGACTCATCCATCTTACGCCCGCAGACTACGACGACTTCGTGAATGCCATTCG CAGCGCTCGCTCCGCATTCTGTTTGACACCCATGGGTATGATGCAGTTCAACGATATATTGCAGAACCTC AAGAGATCCAAACAGACTAAGGAGCTGTGGCAACGGGTAAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223577 representing NM_015037
Red=Cloning site Green=Tags(s) MELMFAEWEDGERFSFEDSDRFEEDSLCSFISEAESLCQNWRGWRKQSAGPNSPTGGGGGGGSGGTRMRD GLVIPLVELSAKQVAFHIPFEVVEKVYPPVPEQLQLRIAFWSFPENEEDIRLYSCLANGSADEFQRGDQL FRMRAVKDPLQIGFHLSATVVPPQMVPPKGAYNVAVMFDRCRVTSCSCTCGAGAKWCTHVVALCLFRIHN ASAVCLRAPVSESLSRLQRDQLQKFAQYLISELPQQILPTAQRLLDELLSSQSTAINTVCGAPDPTAGPS ASDQSTWYLDESTLTDNIKKTLHKFCGPSPVVFSDVNSMYLSSTEPPAAAEWACLLRPLRGREPEGVWNL LSIVREMFKRRDSNAAPLLEILTDQCLTYEQITGWWYSVRTSASHSSASGHTGRSNGQSEVAAHACASMC DEMVTLWRLAVLDPALSPQRRRELCTQLRQWQLKVIENVKRGQHKKTLERLFPGFRPAVEACYFNWEEAY PLPGVTYSGTDRKLALCWARALPSRPGASRSGGLEESRDRPRPLPTEPAVRPKEPGTKRKGLGEGVPSSQ RGPRRLSAEGGDKALHKMGPGGGKAKALGGAGSGSKGSAGGGSKRRLSSEDSSLEPDLAEMSLDDSSLAL GAEASTFGGFPESPPPCPLHGGSRGPSTFLPEPPDTYEEDGGVYFSEGPEPPTASVGPPGLLPGDVCTQD DLPSTDESGNGLPKTKEAAPAVGEEDDDYQAYYLNAQDGAGGEEEKAEGGAGEEHDLFAGLKPLEQESRM EVLFACAEALHAHGYSSEASRLTVELAQDLLANPPDLKVEPPPAKGKKNKVSTSRQTWVATNTLSKAAFL LTVLSERPEHHNLAFRVGMFALELQRPPASTKALEVKLAYQESEVAALLKKIPLGPSEMSTMRCRAEELR EGTLCDYRPVLPLMLASFIFDVLCAPVVSPTGSRPPSRNWNSETPGDEELGFEAAVAALGMKTTVSEAEH PLLCEGTRREKGDLALALMITYKDDQAKLKKILDKLLDRESQTHKPQTLSSFYSSSRPTTASQRSPSKHG GPSAPGALQPLTSGSAGPAQPGSVAGAGPGPTEGFTEKNVPESSPHSPCEGLPSEAALTPRPEGKVPSRL ALGSRGGYNGRGWGSPGRPKKKHTGMASIDSSAPETTSDSSPTLSRRPLRGGWAPTSWGRGQDSDSISSS SSDSLGSSSSSGSRRASASGGARAKTVEVGRYKGRRPESHAPHVPNQPSEAAAHFYFELAKTVLIKAGGN SSTSIFTHPSSSGGHQGPHRNLHLCAFEIGLYALGLHNFVSPNWLSRTYSSHVSWITGQAMEIGSAALTI LVECWDGHLTPPEVASLADRASRARDSNMVRAAAELALSCLPHAHALNPNEIQRALVQCKEQDNLMLEKA CMAVEEAAKGGGVYPEVLFEVAHQWFWLYEQTAGGSSTAREGATSCSASGIRAGGEAGRGMPEGRGGPGT EPVTVAAAAVTAAATVVPVISVGSSLYPGPGLGHGHSPGLHPYTALQPHLPCSPQYLTHPAHPAHPMPHM PRPAVFPVPSSAYPQGVHPAFLGAQYPYSVTPPSLAATAVSFPVPSMAPITVHPYHTEPGLPLPTSVACE LWGQGTVSSVHPASTFPAIQGASLPALTTQPSPLVSGGFPPPEEETHSQPVNPHSLHHLHAAYRVGMLAL EMLGRRAHNDHPNNFSRSPPYTDDVKWLLGLAAKLGVNYVHQFCVGAAKGVLSPFVLQEIVMETLQRLSP AHAHNHLRAPAFHQLVQRCQQAYMQYIHHRLIHLTPADYDDFVNAIRSARSAFCLTPMGMMQFNDILQNL KRSKQTKELWQRVS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015037 |
| ORF Size | 5502 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015037.2, NP_055852.2 |
| RefSeq Size | 6077 bp |
| RefSeq ORF | 5529 bp |
| Locus ID | 23053 |
| UniProt ID | A7E2V4 |
| Gene Summary | Substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex that promotes target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs) (PubMed:33184234, PubMed:33184237). The SCF-like E3 ubiquitin-protein ligase complex acts by catalyzing ubiquitination and subsequent degradation of AGO proteins (AGO1, AGO2, AGO3 and/or AGO4), thereby exposing miRNAs for degradation (PubMed:33184234, PubMed:33184237). Specifically recognizes and binds AGO proteins when they are engaged with a TDMD target (PubMed:33184234). May also acts as a regulator of axon guidance: specifically recognizes misfolded ROBO3 and promotes its ubiquitination and subsequent degradation (PubMed:24012004).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
You may also need
Customer
Reviews
Loading...
