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C10orf90 (NM_001004298) Human Tagged ORF Clone
CAT＃: RG223255
- TrueORF®
C10orf90 (tGFP-tagged) - Human chromosome 10 open reading frame 90 (C10orf90)
ORF Plasmid: DDK
"NM_001004298" in other vectors (4)
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CNY 4,370.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | bA422P15.2; FATS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223255 representing NM_001004298
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAAACTGTCTGGAGAAGGATTACGGGACAGCTACCACAGTAGAAGAGATCAAATTGCCCTTAAAA ATCTCCAGTCTGATGTCACAGAGGCAAAATCTGACTTCACCAAGGAGACCCTGGCATCACAAAACACAAA AATGATTTCATCCATAGTCATCTCCCAGATGATTGATGAGAATAAGTCAAGAGAAAACAGGGCCTCCTTG CCCCTACCGTGTGCCATTGCTCAGTCTCGTGCACATCACGCCAAGCAATCTCTGGCTAACCGCAGCGGAG TCAACATTCACAGAGCATTTGCGTTACTTCCGGGCAGATTAGGAATCCCGGCACCGTCAGATGAGCGAGG ACCTGAGGCAGAGCTGCCGCCCAAAGAGGAGAGACCCTGTGGGGGCCCCCGCAGAGGGTTTGCATCCATC ACCATCACGGCCAGACGCGTGGGCCCCCCAGCCCGCGCCCTGGTGTGGGGGACTGCTGGGGACTCTCTGT GCCCCAAGTGCAGGGCTGAGGACACACTGTTCCAGGCGCCCCCGGCTCTGGCCAATGGCGCCCATCCAGG TCGGCATCAGAGATCTTTTGCCTGCACAGAGTTCTCCAGAAACAGCTCCGTGGTGCGGCTGAAGGTTCCC GAGGCCCACACTGGGTTGTGTGAGAGACGCAAGTACTGGGTCACCCATGCAGACGACAAAGAGACCAGTT TTTCTCCAGACACCCCACTGTCAGGAAAGAGCCCGCTGGTGTTCAGTTCCTGTGTCCACCTCAGGGTGTC TCAGCAGTGTCCAGATTCAATCTATTACGTAGACAAGTCTCTCTCCGTCCCCATTGAGCCACCTCAAATT GCCAGCCCCAAAATGCACAGATCCGTCCTGTCGCTCAACCTCAATTGTAGTTCCCACAGATTAACAGCAG ATGGAGTAGATGGCCTAGTGAACAGAGAGCCAATAAGCGAAGCCCTGAAGCAGGAGCTCCTGGAGGGAGA CCAGGACCTCGTAGGCCAGCGCTGGAACCCAGGTTTACAAGAAAGTCACTTGAAGGAAACCCCATCGTTG AGGCGGGTGCATTTGGGGACCGGCGCTTGTCCTTGGAGTGGTTCTTTTCCATTGGAAAACACAGAATTGG CAAATGTGGGAGCTAACCAAGTCACTGTAAGAAAAGGGGAAAAGGACCATACAACTCATTGTCACGCCAG CGATCATGCCAACCAACTGTCCATTCACATTCCTGGCTGGAGTTACAGGGCAGTACACACAAAAGTATTT TCTGGAAGCAGCAAGAGGCAACAAGGAGAAGTATGTATGACTGTGTCTGCTCCTCCAGTGGAACAGAAGC CCACTAGACATTTCCTTCCCATTGGGGATAGCTCTCCAAGCGATGACTGTCTGTCTAGAGACCTTTCTGA GCCCACAGAGAGACGACATCAAAGCTTCCTGAAACCCAGAATCCTTTTTCCTGGGTTTCTTTGCCCCTTA CAAGATGTATGTGCATCTCTGCAGGAAGACAATGGTGTTCAGATAGAAAGCAAGTTCCCCAAAGGAGATT ACACATGCTGTGACTTGGTTGTAAAAATAAAGGAATGTAAGAAGAGTGAGGACCCCACCACACCTGAGCC CTCCCCAGCAGCACCCTCGCCAGCACCCCGCGATGGAGCAGGGAGCCCTGGCCTGTCCGAAGACTGTTCT GAGTCTCAGCAAACGCCTGCAAGATCTTTGACCTTGCAAGAAGCACTGGAAGTTCGTAAGCCTCAGTTCA TTTCTCGCTCACAAGAACGGCTGAAGAAGCTTGAACACATGGTCCAGCAGAGGAAAGCCCAGCGGAAGGA GGACCTGAGGCAGAAGCAGAGCCTCCTTCCCATCCGCACCAGCAAGAAGCAGTTCACGATTCCCCATCCT CTGAGTGATAACTTGTTCAAACCCAAAGAACGGTGCATTTCAGAGAAGGAGATGCATATGCGATCTAAGA GAATCTATGATAACTTGCCGGAAGTTAAAAAGAAAAAAGAAGAACAAAGGAAAAGGGTGATCTTACAAAG TAACAGACTCCGTGCCGAAGTCTTCAAAAAGCAATTACTGGACCAGCTCCTTCAAAGGAATGCGGTC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223255 representing NM_001004298
Red=Cloning site Green=Tags(s) MLKLSGEGLRDSYHSRRDQIALKNLQSDVTEAKSDFTKETLASQNTKMISSIVISQMIDENKSRENRASL PLPCAIAQSRAHHAKQSLANRSGVNIHRAFALLPGRLGIPAPSDERGPEAELPPKEERPCGGPRRGFASI TITARRVGPPARALVWGTAGDSLCPKCRAEDTLFQAPPALANGAHPGRHQRSFACTEFSRNSSVVRLKVP EAHTGLCERRKYWVTHADDKETSFSPDTPLSGKSPLVFSSCVHLRVSQQCPDSIYYVDKSLSVPIEPPQI ASPKMHRSVLSLNLNCSSHRLTADGVDGLVNREPISEALKQELLEGDQDLVGQRWNPGLQESHLKETPSL RRVHLGTGACPWSGSFPLENTELANVGANQVTVRKGEKDHTTHCHASDHANQLSIHIPGWSYRAVHTKVF SGSSKRQQGEVCMTVSAPPVEQKPTRHFLPIGDSSPSDDCLSRDLSEPTERRHQSFLKPRILFPGFLCPL QDVCASLQEDNGVQIESKFPKGDYTCCDLVVKIKECKKSEDPTTPEPSPAAPSPAPRDGAGSPGLSEDCS ESQQTPARSLTLQEALEVRKPQFISRSQERLKKLEHMVQQRKAQRKEDLRQKQSLLPIRTSKKQFTIPHP LSDNLFKPKERCISEKEMHMRSKRIYDNLPEVKKKKEEQRKRVILQSNRLRAEVFKKQLLDQLLQRNAV SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001004298 |
| ORF Size | 2097 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001004298.3 |
| RefSeq Size | 3097 bp |
| RefSeq ORF | 2100 bp |
| Locus ID | 118611 |
| UniProt ID | Q96M02 |
| Gene Summary | Tumor suppressor that is required to sustain G2/M checkpoint after DNA damage. Acts as a p53/TP53 activator by inhibiting MDM2 binding to p53/TP53 and stimulating non-proteolytic polyubiquitination of p53/TP53. Exhibits ubiquitin ligase (E3) activity and assemble ubiquitin polymers through 'Lys-11'- (K11-), 'Lys-29'- (K29-) and 'Lys-63'- (K63)-linkages, independently of the ubiquitin-conjugating enzyme (E2). Promotes p53/TP53-dependent transcription of CDKN1A/p21, leading to robust checkpoint response. Mediates CDKN1A/p21 protein stability in a ubiquitin-independent manner. Interacts with HDAC1 and prevents binding of HDAC1 to CDKN1A/p21 and facilitates the acetylation and stabilization of CDKN1A/p21 (By similarity). May have a role in the assembly of primary cilia (Probable).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A functional genetic variant in fragile-site gene FATS modulates the risk of breast cancer in triparous women
,null,
BMC Cancer
,PubMed ID 26223354
[C10orf90]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223255 | C10orf90 (Myc-DDK-tagged)-Human chromosome 10 open reading frame 90 (C10orf90) |
CNY 3,990.00 |
|
| RC223255L3 | Lenti ORF clone of Human chromosome 10 open reading frame 90 (C10orf90), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223255L4 | Lenti ORF clone of Human chromosome 10 open reading frame 90 (C10orf90), mGFP tagged |
CNY 5,890.00 |
|
| SC300618 | C10orf90 (untagged)-Human chromosome 10 open reading frame 90 (C10orf90) |
CNY 5,128.00 |
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