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TNK1 (NM_003985) Human Tagged ORF Clone
CAT＃: RG223073
- TrueORF®
TNK1 (tGFP-tagged) - Human tyrosine kinase, non-receptor, 1 (TNK1)
ORF Plasmid: DDK
"NM_003985" in other vectors (7)
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CNY 6,520.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | KOS1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223073 representing NM_003985
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCCTGAGGCTGGCTCTCTGTGGCTACTGAAGCTGCTCCGGGACATCCAGTTGGCCCAGTTTTACT GGCCCATCCTTGAGGAGCTTAATGTCACTCGGCCAGAGCACTTCGACTTTGTAAAGCCTGAGGACCTGGA CGGCATTGGCATGGGCCGGCCTGCCCAGCGCAGACTGTCCGAAGCTCTGAAAAGGCTACGTTCTGGGCCT AAGTCTAAGAACTGGGTCTACAAGATCCTTGGAGGTTTTGCCCCTGAGCACAAGGAGCCCACCCTGCCCT CGGACAGCCCACGGCACCTCCCTGAGCCAGAGGGGGGCCTCAAGTGTCTGATCCCAGAGGGTGCTGTTTG CAGAGGGGAGCTGCTGGGTTCAGGCTGCTTCGGTGTGGTGCACCGAGGGCTGTGGACGCTGCCCAGTGGC AAGAGTGTCCCAGTGGCTGTCAAGTCCCTCCGGGTAGGTCCCGAAGGCCCGATGGGCACAGAACTGGGGG ACTTCCTGCGAGAGGTATCGGTCATGATGAACTTGGAGCACCCACACGTGCTGCGTCTGCACGGCCTTGT ACTGGGCCAGCCTCTGCAGATGGTGATGGAGCTGGCGCCACTGGGCTCCCTGCACGCGCGCCTAACGGCC CCGGCCCCGACACCCCCGCTGCTCGTGGCCCTGCTCTGCCTCTTCCTGCGGCAGCTGGCGGGAGCCATGG CGTACCTGGGGGCCCGCGGGCTGGTGCACCGAGACCTCGCTACGCGCAACCTACTGCTGGCGTCGCCGCG CACCATCAAGGTGGCTGACTTCGGGCTGGTGCGGCCTCTGGGCGGTGCCCGGGGCCGCTACGTCATGGGC GGGCCCCGCCCTATCCCCTACGCCTGGTGTGCCCCAGAGAGCCTGCGCCACGGAGCCTTCTCGTCTGCCT CGGACGTGTGGATGTTTGGGGTGACGCTGTGGGAGATGTTCTCCGGGGGCGAGGAACCCTGGGCCGGGGT CCCACCGTACCTCATCCTGCAGCGGCTGGAGGACAGAGCCCGGCTGCCTAGGCCTCCCCTCTGCTCCAGG GCCCTCTACTCCCTCGCCTTGCGCTGCTGGGCCCCCCACCCTGCCGACCGGCCTAGCTTTTCCCACCTGG AGGGGCTGCTGCAAGAGGCCGGGCCTTCGGAAGCATGTTGTGTGAGGGATGTCACAGAACCAGGCGCCCT GAGGATGGAGACTGGTGACCCCATCACAGTCATCGAGGGCAGCCCCGACTCCACAATCTGGAAGGGCCAG AATGGTCGCACCTTCAAAGTGGGCAGCTTCCCAGCCTCGGCAGTGACGCTGGCAGATGCGGGGGGCTTGC CAGCCACCCGTCCAGTCCACAGAGGCACCCCTGCCCGGGGAGATCAACACCCAGGAAGCATAGATGGAGA CAGAAAGAAGGCAAATCTTTGGGATGCGCCCCCAGCACGGGGCCAGAGGAGGAACATGCCCCTGGAGAGG ATGAAAGGCATTTCCAGGAGTCTGGAGTCAGTTCTGTCCCTCGGTCCTCGTCCCACAGGGGGTGGTTCAA GCCCCCCTGAAATTCGACAAGCCAGAGCTGTGCCCCAGGGACCTCCAGGCCTGCCTCCACGCCCACCTTT ATCCTCTAGCTCTCCTCAGCCCAGCCAGCCCTCTAGGGAGAGGCTTCCCTGGCCCAAAAGAAAACCCCCA CACAATCACCCCATGGGAATGCCTGGAGCCCGTAAAGCCGCTGCCCTCTCTGGAGGCCTCTTGTCCGATC CTGAGTTGCAGAGGAAGATTATGGAGATGGAGCTGAGTGTGCATGGGGTCACCCACCAGGAGTGCCAGAC AGCACTAGGAGCCACTGGGGGAGATGTGGTTTCTGCCATCCGGAACCTCAAGGTAGATCAGCTCTTCCAC CTGAGTAGCCGGTCCAGAGCTGACTGCTGGCGCATCCTGGAGCATTACCAGTGGGACCTCTCAGCTGCCA GCCGCTATGTCCTGGCCAGGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223073 representing NM_003985
Red=Cloning site Green=Tags(s) MLPEAGSLWLLKLLRDIQLAQFYWPILEELNVTRPEHFDFVKPEDLDGIGMGRPAQRRLSEALKRLRSGP KSKNWVYKILGGFAPEHKEPTLPSDSPRHLPEPEGGLKCLIPEGAVCRGELLGSGCFGVVHRGLWTLPSG KSVPVAVKSLRVGPEGPMGTELGDFLREVSVMMNLEHPHVLRLHGLVLGQPLQMVMELAPLGSLHARLTA PAPTPPLLVALLCLFLRQLAGAMAYLGARGLVHRDLATRNLLLASPRTIKVADFGLVRPLGGARGRYVMG GPRPIPYAWCAPESLRHGAFSSASDVWMFGVTLWEMFSGGEEPWAGVPPYLILQRLEDRARLPRPPLCSR ALYSLALRCWAPHPADRPSFSHLEGLLQEAGPSEACCVRDVTEPGALRMETGDPITVIEGSPDSTIWKGQ NGRTFKVGSFPASAVTLADAGGLPATRPVHRGTPARGDQHPGSIDGDRKKANLWDAPPARGQRRNMPLER MKGISRSLESVLSLGPRPTGGGSSPPEIRQARAVPQGPPGLPPRPPLSSSSPQPSQPSRERLPWPKRKPP HNHPMGMPGARKAAALSGGLLSDPELQRKIMEMELSVHGVTHQECQTALGATGGDVVSAIRNLKVDQLFH LSSRSRADCWRILEHYQWDLSAASRYVLARP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003985 |
| ORF Size | 1983 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003985.1, NP_003976.1 |
| RefSeq Size | 2771 bp |
| RefSeq ORF | 1986 bp |
| Locus ID | 8711 |
| UniProt ID | Q13470 |
| Domains | pkinase, TyrKc, SH3, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene belongs to the tyrosine protein kinase family. Tyrosine protein kinases are important regulators of intracellular signal transduction pathways, mediating cellular proliferation, survival, and development. This gene is highly expressed in fetal tissues and at lower levels in few adult tissues, thus may function in signaling pathways utilized broadly during fetal development, and more selectively in adult tissues. It plays a negative regulatory role in the Ras-Raf1-MAPK pathway, and knockout mice have been shown to develop spontaneous tumors, suggesting a role as a tumor suppressor gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223073 | TNK1 (Myc-DDK-tagged)-Human tyrosine kinase, non-receptor, 1 (TNK1) |
CNY 4,920.00 |
|
| RC223073L1 | Lenti ORF clone of Human tyrosine kinase, non-receptor, 1 (TNK1), Myc-DDK-tagged |
CNY 7,320.00 |
|
| RC223073L2 | Lenti ORF clone of Human tyrosine kinase, non-receptor, 1 (TNK1), mGFP tagged |
CNY 5,890.00 |
|
| RC223073L3 | Lenti ORF clone of Human tyrosine kinase, non-receptor, 1 (TNK1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223073L4 | Lenti ORF clone of Human tyrosine kinase, non-receptor, 1 (TNK1), mGFP tagged |
CNY 7,320.00 |
|
| SC117643 | TNK1 (untagged)-Human tyrosine kinase, non-receptor, 1 (TNK1) |
CNY 4,888.00 |
|
| SC323530 | TNK1 (untagged)-Kinase deficient mutant (K148M) of Human tyrosine kinase, non-receptor, 1 (TNK1) |
CNY 4,888.00 |
