SP140 (NM_007237) Human Tagged ORF Clone
CAT#: RG222828
- TrueORF®
SP140 (tGFP-tagged) - Human SP140 nuclear body protein (SP140), transcript variant 1
ORF Plasmid: DDK
"NM_007237" in other vectors (4)
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CNY 7,890.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | LYSP100; LYSP100-A; LYSP100-B |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG222828 representing NM_007237
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGCAGGGCCAGCAGGGGCAGATGGCAAGTGGAGACAGCAATCTCAACTTCAGGATGGTCGCAG AGATCCAGAACGTAGAGGGTCAGAACCTGCAGGAGCAGGTTTGCCCTGAGCCCATTTTCAGGTTCTTCAG AGAAAACAAGGTGGAGATTGCAAGTGCAATAACAAGGCCATTTCCTTTCCTTATGGGCCTCCGAGACCGC TCCTTCATCTCCGAGCAGATGTATGAACATTTTCAAGAAGCTTTTAGAAACCTGGTCCCAGTGACAAGAG TGATGTATTGTGTACTCAGTGAACTGGAGAAGACATTTGGCTGGTCACATCTGGAAGCATTGTTCAGCAG GATTAACCTGATGGCCTATCCTGATTTAAACGAGATTTACAGAAGCTTCCAGAATGTATGCTATGAACAC TCACCTCTCCAAATGAATAATGTAAACGATTTAGAAGATAGACCCAGATTACTACCATATGGTAAACAAG AGAACAGCAATGCCTGTCATGAAATGGATGATATAGCAGTGCCTCAGGAAGCCTTGAGCTCCTCGCCAAG GTGTGAGCCAGGTTTCTCTTCAGAGTCTTGTGAGCAGTTAGCTCTCCCAAAGGCTGGTGGAGGAGATGCT GAAGATGCACCCAGCCTACTACCAGGTGGGGGAGTGTCCTGTAAACTTGCTATACAAATAGATGAAGGAG AATCAGAAGAAATGCCCAAGTTACTGCCTTATGATACAGAAGTTCTAGAAAGCAACGGGATGATAGATGC GGCAAGGACATACAGCACAGCACCAGGGGAGAAACAGGGAGAGGAGGAAGGCAGGAACAGTCCCAGAAAA AGAAACCAAGACAAGGAGAAGTACCAAGAGAGTCCAGAGGGAAGAGACAAAGAGACCTTTGATCTAAAAA CTCCCCAAGTCACTAATGAAGGAGAACCAGAGAAGGGGCTCTGTCTACTACCAGGTGAAGGAGAAGAGGG CAGTGATGACTGTTCAGAAATGTGTGATGGAGAAGAGCCCCAGGAAGCCTCTAGCTCCCTAGCAAGATGT GGGTCAGTTTCTTGTTTCTCTACAGAGACCTTTGATCTAAAGACTCCCCAAGTCACTAATGAAGGAGAAC CAGAGAAGGAGCTCAGTCTACTACCAGGTGAAGGAGAAGAGGGCAGTGATGACTGTTCGGAAATGTGTGA TGGAGAAGAGCGCCAGGAAGCCTCTAGCTCCCTAGCAAGACGTGGGTCAGTGTCTAGTGAACTAGAAAAT CACCCAATGAATGAAGAAGGAGAATCAGAAGAGCTTGCTTCTAGCCTGCTATATGATAATGTACCAGGAG CGGAGCAATCAGCATATGAAAATGAGAAGTGTTCCTGTGTCATGTGTTTCTCAGAAGAGGTGCCAGGAAG CCCAGAAGCAAGGACGGAAAGTGATCAAGCGTGTGGCACAATGGATACTGTGGATATTGCAAACAACTCC ACTTTGGGAAAACCCAAGAGGAAAAGAAGAAAAAAGAGGGGGCATGGCTGGAGCAGAATGAGAATGAGAA GGCAGAAAAACAGCCAACAAAATGATAATAGCAAAGCCGACGGCCAGGTGGTCTCCAGTGAAAAGAAGGC GAACGTGAATCTGAAAGACCTTTCCAAGATTAGGGGGAGAAAGAGAGGCAAACCTGGAACCCGCTTCACT CAGAGTGACAGAGCTGCACAGAAAAGAGTCCGATCAAGAGCTTCAAGAAAGCACAAAGATGAAACTGTGG ATTTTAAGGCTCCTTTGCTTCCAGTGACCTGTGGTGGGGTGAAGGGAATTTTACATAAGAAGAAATTGCA GCAAGGAATCTTGGTGAAGTGTATACAGACTGAGGATGGAAAATGGTTCACCCCCACGGAATTTGAAATC AAAGGAGGCCATGCAAGATCAAAGAACTGGAGGCTGAGTGTGCGCTGTGGCGGGTGGCCCCTACGATGGC TGATGGAGAATGGATTTCTGCCTGATCCTCCAAGAATACGTTACAGGAAAAAAAAGAGAATACTGAAGTC TCAAAACAATAGCTCAGTTGACCCTTGTATGAGAAACCTGGATGAGTGTGAGGTGTGCCGGGACGGAGGG GAGCTGTTCTGTTGCGACACTTGTTCAAGAGTCTTCCATGAGGACTGTCACATCCCGCCTGTGGAAGCTG AGAGGACCCCGTGGAATTGCATCTTCTGCAGGATGAAGGAGTCTCCGGGAAGCCAACAGTGTTGTCAGGA ATCTGAGGTCCTGGAGAGGCAGATGTGTCCTGAGGAACAGTTGAAATGTGAGTTCCTCCTCTTGAAAGTC TATTGCTGTTCTGAGAGCTCCTTTTTTGCCAAGATTCCATACTATTATTATATTAGAGAGGCGTGTCAAG GCCTGAAGGAGCCCATGTGGTTGGATAAAATCAAGAAAAGGCTGAATGAGCACGGTTACCCCCAAGTGGA GGGGTTTGTACAAGACATGCGCCTCATCTTCCAGAACCACAGGGCCTCTTACAAGTACAAGGATTTTGGC CAAATGGGATTTAGACTGGAGGCTGAGTTTGAGAAGAATTTCAAGGAAGTGTTTGCTATTCAGGAAACAA ATGGGAACAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222828 representing NM_007237
Red=Cloning site Green=Tags(s) MAQQGQQGQMASGDSNLNFRMVAEIQNVEGQNLQEQVCPEPIFRFFRENKVEIASAITRPFPFLMGLRDR SFISEQMYEHFQEAFRNLVPVTRVMYCVLSELEKTFGWSHLEALFSRINLMAYPDLNEIYRSFQNVCYEH SPLQMNNVNDLEDRPRLLPYGKQENSNACHEMDDIAVPQEALSSSPRCEPGFSSESCEQLALPKAGGGDA EDAPSLLPGGGVSCKLAIQIDEGESEEMPKLLPYDTEVLESNGMIDAARTYSTAPGEKQGEEEGRNSPRK RNQDKEKYQESPEGRDKETFDLKTPQVTNEGEPEKGLCLLPGEGEEGSDDCSEMCDGEEPQEASSSLARC GSVSCFSTETFDLKTPQVTNEGEPEKELSLLPGEGEEGSDDCSEMCDGEERQEASSSLARRGSVSSELEN HPMNEEGESEELASSLLYDNVPGAEQSAYENEKCSCVMCFSEEVPGSPEARTESDQACGTMDTVDIANNS TLGKPKRKRRKKRGHGWSRMRMRRQKNSQQNDNSKADGQVVSSEKKANVNLKDLSKIRGRKRGKPGTRFT QSDRAAQKRVRSRASRKHKDETVDFKAPLLPVTCGGVKGILHKKKLQQGILVKCIQTEDGKWFTPTEFEI KGGHARSKNWRLSVRCGGWPLRWLMENGFLPDPPRIRYRKKKRILKSQNNSSVDPCMRNLDECEVCRDGG ELFCCDTCSRVFHEDCHIPPVEAERTPWNCIFCRMKESPGSQQCCQESEVLERQMCPEEQLKCEFLLLKV YCCSESSFFAKIPYYYYIREACQGLKEPMWLDKIKKRLNEHGYPQVEGFVQDMRLIFQNHRASYKYKDFG QMGFRLEAEFEKNFKEVFAIQETNGNN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007237 |
ORF Size | 2601 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007237.3, NP_009168.3 |
RefSeq Size | 3269 bp |
RefSeq ORF | 2604 bp |
Locus ID | 11262 |
UniProt ID | Q13342 |
Domains | SAND, BROMO, PHD, Sp100 |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | This gene encodes a member of the SP100 family of proteins, which are share common domains including an N-terminal homogeneously staining region domain followed by a SP100/autoimmune regulator/NucP41/P75/deformed epidermal autoregulatory factor domain, a plant homeobox zinc finger, and a bromodomain. The encoded protein is interferon-inducible and is expressed at high levels in the nuclei of leukocytes. Variants of this gene have been associated with multiple sclerosis, Crohn's disease, and chronic lymphocytic leukemia. Alternative splicing results in multiple variants. [provided by RefSeq, Aug 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222828 | SP140 (Myc-DDK-tagged)-Human SP140 nuclear body protein (SP140), transcript variant 1 |
CNY 6,352.00 |
|
RC222828L3 | Lenti-ORF clone of SP140 (Myc-DDK-tagged)-Human SP140 nuclear body protein (SP140), transcript variant 1 |
CNY 9,030.00 |
|
RC222828L4 | Lenti-ORF clone of SP140 (mGFP-tagged)-Human SP140 nuclear body protein (SP140), transcript variant 1 |
CNY 9,030.00 |
|
SC318996 | SP140 (untagged)-Human SP140 nuclear body protein (SP140), transcript variant 1 |
CNY 16,720.00 |