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Rapsyn (RAPSN) (NM_005055) Human Tagged ORF Clone
CAT＃: RG222710
- TrueORF®
RAPSN (tGFP-tagged) - Human receptor-associated protein of the synapse (RAPSN), transcript variant 1
ORF Plasmid: DDK
"NM_005055" in other vectors (6)
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CNY 5,256.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CMS4C; CMS11; FADS; FADS2; RAPSYN; RNF205 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222710 representing NM_005055
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAGGACCAGACCAAGCAGCAGATCGAGAAGGGGCTCCAGCTGTACCAGTCCAACCAGACAGAGA AGGCATTGCAGGTGTGGACAAAGGTGCTGGAGAAGAGCTCGGACCTCATGGGGCGCTTCCGCGTGCTGGG CTGCCTGGTCACAGCCCACTCGGAGATGGGCCGCTACAAGGAGATGCTGAAGTTCGCTGTGGTCCAGATC GACACGGCCCGGGAGCTGGAGGATGCCGACTTCCTCCTGGAGAGCTACCTGAACCTGGCACGCAGCAACG AGAAGCTGTGCGAGTTTCACAAGACCATCTCCTACTGCAAGACCTGCCTTGGGCTGCCTGGTACCAGGGC AGGTGCCCAGCTCGGAGGCCAGGTCAGCCTGAGCATGGGCAATGCCTTCCTGGGCCTCAGCGTCTTCCAG AAGGCCCTGGAGAGCTTCGAGAAGGCCCTGCGCTATGCCCACAACAATGATGACGCCATGCTCGAGTGCC GCGTGTGCTGCAGCCTGGGCAGCTTCTATGCCCAGGTCAAGGACTACGAGAAAGCCCTGTTCTTCCCCTG CAAGGCGGCAGAGCTTGTCAACAACTATGGCAAAGGCTGGAGCCTGAAGTACCGGGCCATGAGCCAGTAC CACATGGCCGTGGCCTATCGCCTGCTGGGCCGCCTGGGCAGTGCCATGGAGTGTTGTGAGGAGTCTATGA AGATCGCGCTGCAGCACGGGGACCGGCCACTGCAGGCGCTCTGCCTGCTCTGCTTCGCTGACATCCACCG GAGCCGTGGGGACCTGGAGACAGCCTTCCCCAGGTACGACTCCGCCATGAGCATCATGACCGAGATCGGA AACCGCCTGGGGCAGGTGCAGGCGCTGCTGGGTGTGGCCAAGTGCTGGGTGGCCAGGAAGGCGCTGGACA AGGCTCTGGATGCCATCGAGAGAGCCCAGGATCTGGCCGAGGAGGTGGGGAACAAGCTGAGCCAGCTCAA GCTGCACTGTCTGAGCGAGAGCATTTACCGCAGCAAAGGGCTGCAGCGGGAACTGCGGGCGCACGTTGTG AGGTTCCACGAGTGCGTGGAGGAGACGGAGCTCTACTGCGGCCTGTGCGGCGAGTCCATAGGCGAGAAGA ACAGCCGGCTGCAGGCCCTACCTTGCTCCCACATCTTCCACCTCAGGTGCCTGCAGAACAACGGGACCCG GAGCTGTCCCAACTGCCGCCGCTCATCCATGAAGCCTGGCTTTGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222710 representing NM_005055
Red=Cloning site Green=Tags(s) MGQDQTKQQIEKGLQLYQSNQTEKALQVWTKVLEKSSDLMGRFRVLGCLVTAHSEMGRYKEMLKFAVVQI DTARELEDADFLLESYLNLARSNEKLCEFHKTISYCKTCLGLPGTRAGAQLGGQVSLSMGNAFLGLSVFQ KALESFEKALRYAHNNDDAMLECRVCCSLGSFYAQVKDYEKALFFPCKAAELVNNYGKGWSLKYRAMSQY HMAVAYRLLGRLGSAMECCEESMKIALQHGDRPLQALCLLCFADIHRSRGDLETAFPRYDSAMSIMTEIG NRLGQVQALLGVAKCWVARKALDKALDAIERAQDLAEEVGNKLSQLKLHCLSESIYRSKGLQRELRAHVV RFHECVEETELYCGLCGESIGEKNSRLQALPCSHIFHLRCLQNNGTRSCPNCRRSSMKPGFV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005055 |
| ORF Size | 1236 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005055.5 |
| RefSeq Size | 1664 bp |
| RefSeq ORF | 1239 bp |
| Locus ID | 5913 |
| UniProt ID | Q13702 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of a family of proteins that are receptor associated proteins of the synapse. The encoded protein contains a conserved cAMP-dependent protein kinase phosphorylation site, and plays a critical role in clustering and anchoring nicotinic acetylcholine receptors at synaptic sites by linking the receptors to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin. Mutations in this gene may play a role in postsynaptic congenital myasthenic syndromes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222710 | RAPSN (Myc-DDK-tagged)-Human receptor-associated protein of the synapse (RAPSN), transcript variant 1 |
CNY 3,656.00 |
|
| RC222710L1 | Lenti ORF clone of Human receptor-associated protein of the synapse (RAPSN), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222710L2 | Lenti ORF clone of Human receptor-associated protein of the synapse (RAPSN), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC222710L3 | Lenti ORF clone of Human receptor-associated protein of the synapse (RAPSN), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222710L4 | Lenti ORF clone of Human receptor-associated protein of the synapse (RAPSN), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC303569 | RAPSN (untagged)-Human receptor-associated protein of the synapse (RAPSN), transcript variant 1 |
CNY 6,650.00 |
