Frizzled 8 (FZD8) (NM_031866) Human Tagged ORF Clone
CAT#: RG222311
- TrueORF®
FZD8 (tGFP-tagged) - Human frizzled family receptor 8 (FZD8)
ORF Plasmid: DDK
"NM_031866" in other vectors (6)
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CNY 9,224.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | FZ-8; hFZ8 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG222311 representing NM_031866
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGGGGTTACCTGTTGGAAGTGACCTCGCTGCTGGCCGCCTTGGCGCTGCTGCAGCGCTCTAGCG GCGCTGCGGCCGCCTCGGCCAAGGAGCTGGCATGCCAAGAGATCACCGTGCCGCTGTGTAAGGGCATCGG CTACAACTACACCTACATGCCCAATCAGTTCAACCACGACACGCAAGACGAGGCGGGCCTGGAGGTGCAC CAGTTCTGGCCGCTGGTGGAGATCCAGTGCTCGCCCGATCTCAAGTTCTTCCTGTGCAGCATGTACACGC CCATCTGCCTAGAGGACTACAAGAAGCCGCTGCCGCCCTGCCGCTCGGTGTGCGAGCGCGCCAAGGCCGG CTGCGCGCCGCTCATGCGCCAGTACGGCTTCGCCTGGCCCGACCGCATGCGCTGCGACCGGCTGCCCGAG CAAGGCAACCCTGACACGCTGTGCATGGACTACAACCGCACCGACCTAACCACCGCCGCGCCCAGCCCGC CGCGCCGCCTGCCGCCGCCGCCGCCCGGCGAGCAGCCGCCTTCGGGCAGCGGCCACGGCCGCCCGCCGGG GGCCAGGCCCCCGCACCGCGGAGGCGGCAGGGGCGGTGGCGGCGGGGACGCGGCGGCGCCCCCAGCTCGC GGCGGCGGCGGTGGCGGGAAGGCGCGGCCCCCTGGCGGCGGCGCGGCTCCCTGCGAGCCCGGGTGCCAGT GCCGCGCGCCTATGGTGAGCGTGTCCAGCGAGCGCCACCCGCTCTACAACCGCGTCAAGACAGGCCAGAT CGCTAACTGCGCGCTGCCCTGCCACAACCCCTTTTTCAGCCAGGACGAGCGCGCCTTCACCGTCTTCTGG ATCGGCCTGTGGTCGGTGCTCTGCTTCGTGTCCACCTTCGCCACCGTCTCCACCTTCCTTATCGACATGG AGCGCTTCAAGTACCCGGAGCGGCCCATTATCTTCCTCTCGGCCTGCTACCTCTTCGTGTCGGTGGGCTA CCTAGTGCGCCTGGTGGCGGGCCACGAGAAGGTGGCGTGCAGCGGTGGCGCGCCGGGCGCGGGGGGCGCT GGGGGCGCGGGCGGCGCGGCGGCGGGCGCGGGCGCGGCGGGCGCGGGCGCGGGCGGCCCGGGCGGGCGCG GCGAGTACGAGGAGCTGGGCGCGGTGGAGCAGCACGTGCGCTACGAGACCACCGGCCCCGCGCTGTGCAC CGTGGTCTTCTTGCTGGTCTACTTCTTCGGCATGGCCAGCTCCATCTGGTGGGTGATCTTGTCGCTCACA TGGTTCCTGGCGGCCGGTATGAAGTGGGGCAACGAAGCCATCGCCGGCTACTCGCAGTACTTCCACCTGG CCGCGTGGCTTGTGCCCAGCGTCAAGTCCATCGCGGTGCTGGCGCTCAGCTCGGTGGACGGCGACCCGGT GGCGGGCATCTGCTACGTGGGCAACCAGAGCCTGGACAACCTGCGCGGCTTCGTGCTGGCGCCGCTGGTC ATCTACCTCTTCATCGGCACCATGTTCCTGCTGGCCGGCTTCGTGTCCCTGTTCCGCATCCGCTCGGTCA TCAAGCAACAGGACGGCCCCACCAAGACGCACAAGCTGGAGAAGCTGATGATCCGCCTGGGCCTGTTCAC CGTGCTCTACACCGTGCCCGCCGCGGTGGTGGTCGCCTGCCTCTTCTACGAGCAGCACAACCGCCCGCGC TGGGAGGCCACGCACAACTGCCCGTGCCTGCGGGACCTGCAGCCCGACCAGGCACGCAGGCCCGACTACG CCGTCTTCATGCTCAAGTACTTCATGTGCCTAGTGGTGGGCATCACCTCGGGCGTGTGGGTCTGGTCCGG CAAGACGCTGGAGTCCTGGCGCTCCCTGTGCACCCGCTGCTGCTGGGCCAGCAAGGGCGCCGCGGTGGGC GGGGGCGCGGGCGCCACGGCCGCGGGGGGTGGCGGCGGGCCGGGGGGCGGCGGCGGCGGGGGACCCGGCG GCGGCGGGGGGCCGGGCGGCGGCGGGGGCTCCCTCTACAGCGACGTCAGCACTGGCCTGACGTGGCGGTC GGGCACGGCGAGCTCCGTGTCTTATCCAAAGCAGATGCCATTGTCCCAGGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222311 representing NM_031866
Red=Cloning site Green=Tags(s) MEWGYLLEVTSLLAALALLQRSSGAAAASAKELACQEITVPLCKGIGYNYTYMPNQFNHDTQDEAGLEVH QFWPLVEIQCSPDLKFFLCSMYTPICLEDYKKPLPPCRSVCERAKAGCAPLMRQYGFAWPDRMRCDRLPE QGNPDTLCMDYNRTDLTTAAPSPPRRLPPPPPGEQPPSGSGHGRPPGARPPHRGGGRGGGGGDAAAPPAR GGGGGGKARPPGGGAAPCEPGCQCRAPMVSVSSERHPLYNRVKTGQIANCALPCHNPFFSQDERAFTVFW IGLWSVLCFVSTFATVSTFLIDMERFKYPERPIIFLSACYLFVSVGYLVRLVAGHEKVACSGGAPGAGGA GGAGGAAAGAGAAGAGAGGPGGRGEYEELGAVEQHVRYETTGPALCTVVFLLVYFFGMASSIWWVILSLT WFLAAGMKWGNEAIAGYSQYFHLAAWLVPSVKSIAVLALSSVDGDPVAGICYVGNQSLDNLRGFVLAPLV IYLFIGTMFLLAGFVSLFRIRSVIKQQDGPTKTHKLEKLMIRLGLFTVLYTVPAAVVVACLFYEQHNRPR WEATHNCPCLRDLQPDQARRPDYAVFMLKYFMCLVVGITSGVWVWSGKTLESWRSLCTRCCWASKGAAVG GGAGATAAGGGGGPGGGGGGGPGGGGGPGGGGGSLYSDVSTGLTWRSGTASSVSYPKQMPLSQV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_031866 |
ORF Size | 2082 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_031866.1, NP_114072.1 |
RefSeq Size | 3195 bp |
RefSeq ORF | 2085 bp |
Locus ID | 8325 |
UniProt ID | Q9H461 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway |
Gene Summary | This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222311 | FZD8 (Myc-DDK-tagged)-Human frizzled family receptor 8 (FZD8) |
CNY 7,624.00 |
|
RC222311L1 | Lenti ORF clone of Human frizzled family receptor 8 (FZD8), Myc-DDK-tagged |
CNY 10,024.00 |
|
RC222311L2 | Lenti ORF clone of Human frizzled family receptor 8 (FZD8), mGFP tagged |
CNY 10,024.00 |
|
RC222311L3 | Lenti ORF clone of Human frizzled family receptor 8 (FZD8), Myc-DDK-tagged |
CNY 7,700.00 |
|
RC222311L4 | Lenti ORF clone of Human frizzled family receptor 8 (FZD8), mGFP tagged |
CNY 7,700.00 |
|
SC305350 | FZD8 (untagged)-Human frizzled family receptor 8 (FZD8) |
CNY 7,632.00 |
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